BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= Q06091
         (175 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

5LJ5-s nrPDB                                                          358   e-100
3WXM-B nrPDB                                                           28   1.5  
1E3H-A nrPDB                                                           28   3.0  
5NNP-A nrPDB                                                           28   3.1  
2Z87-A nrPDB                                                           27   3.2  
5JCP-B nrPDB                                                           27   4.0  
5C2K-A nrPDB                                                           27   4.8  
5ZHX-e nrPDB                                                           27   5.1  

>5LJ5-s nrPDB
          Length = 175

 Score =  358 bits (918), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 175/175 (100%), Positives = 175/175 (100%)

Query: 1   MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNA 60
           MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNA
Sbjct: 1   MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNA 60

Query: 61  LYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSY 120
           LYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSY
Sbjct: 61  LYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSY 120

Query: 121 LKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL 175
           LKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL
Sbjct: 121 LKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL 175


>3WXM-B nrPDB
          Length = 376

 Score = 28.5 bits (62), Expect = 1.5,   Method: Compositional matrix adjust.
 Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 6/47 (12%)

Query: 87  FLEEYRRKYPRI-DTSRYIPNESSEVSLLGIVDSYLKHQEIVLDTLL 132
            LEE+ R+  +  DT  Y P E   V+ +G VD+ L     ++DTLL
Sbjct: 276 VLEEFLRRIAKSRDTVAYTPGEVLAVARMGAVDTVL-----LVDTLL 317


>1E3H-A nrPDB
          Length = 757

 Score = 27.7 bits (60), Expect = 3.0,   Method: Composition-based stats.
 Identities = 23/93 (24%), Positives = 38/93 (40%), Gaps = 2/93 (2%)

Query: 43  ILAKRKGADNSVSTLTNALYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSR 102
           +L + +G +  +S    AL    +++R   +++  D     D   L       PR+  S 
Sbjct: 323 LLPEFEGREKEISAAYRALTKSLVRERVIAEKKRIDGRGVTDIRTLAAEVEAIPRVHGSA 382

Query: 103 YIPNESSEVSLLGIVDSYLKHQEIVLDTLLPQT 135
               E  E  +LG+    +   E  LDTL P T
Sbjct: 383 LF--ERGETQILGVTTLNMLRMEQQLDTLSPVT 413


>5NNP-A nrPDB
          Length = 745

 Score = 27.7 bits (60), Expect = 3.1,   Method: Compositional matrix adjust.
 Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 6/71 (8%)

Query: 89  EEYRRKYPRIDTSRYIP-NESSEVSLLGIVDSYLKHQEIVLDTLLPQTVSNQWRINNDYI 147
           +EY  KYPR D ++ +P N  S         +YL    ++ D  +P T +N   + +D  
Sbjct: 281 DEYAAKYPRSDAAKRLPLNFLSGERFRTTAKAYLT---LMFDKGVPSTFANLKHLYSDSF 337

Query: 148 RQ--TCTIVEE 156
           ++    ++ EE
Sbjct: 338 KKETLASLAEE 348


>2Z87-A nrPDB
          Length = 624

 Score = 27.3 bits (59), Expect = 3.2,   Method: Compositional matrix adjust.
 Identities = 20/80 (25%), Positives = 39/80 (48%), Gaps = 10/80 (12%)

Query: 14  DGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNALYTEYLKQRNNKK 73
           DG  ++  +++++ +AN      HP +R I  K KG  ++ +T        Y+ Q ++  
Sbjct: 411 DGSTDDTLRILQEHYAN------HPRVRFISQKNKGIGSASNTAVRLCRGFYIGQLDSDD 464

Query: 74  RRTPDFNDDDDTLFLEEYRR 93
              PD  +    L L+E+R+
Sbjct: 465 FLEPDAVE----LCLDEFRK 480


>5JCP-B nrPDB
          Length = 419

 Score = 27.3 bits (59), Expect = 4.0,   Method: Compositional matrix adjust.
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 21  DQLVKKEFANIKREPVHPEIRGILAKRKGA 50
           D+  ++E A +K+EPV PE    +A R GA
Sbjct: 362 DEHTRRELAKMKQEPVKPEEGRDMANRIGA 391


>5C2K-A nrPDB
          Length = 415

 Score = 26.9 bits (58), Expect = 4.8,   Method: Compositional matrix adjust.
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 21  DQLVKKEFANIKREPVHPEIRGILAKRKGA 50
           D+  ++E A +K+EPV PE    +A R GA
Sbjct: 131 DEHTRRELAKMKQEPVKPEEGRDMANRIGA 160


>5ZHX-e nrPDB
          Length = 198

 Score = 26.9 bits (58), Expect = 5.1,   Method: Compositional matrix adjust.
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 21  DQLVKKEFANIKREPVHPEIRGILAKRKGA 50
           D+  ++E A +K+EPV PE    +A R GA
Sbjct: 129 DEHTRRELAKMKQEPVKPEEGRDMANRIGA 158


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

5LJ5-s nrPDB                                                          347   2e-96

Sequences not found previously or not previously below threshold:

5ZHX-e nrPDB                                                           30   0.70 
5JCP-B nrPDB                                                           30   0.70 
5C2K-A nrPDB                                                           30   0.70 
3WXM-B nrPDB                                                           28   1.8  
1E3H-A nrPDB                                                           28   2.0  
4F7K-A nrPDB                                                           28   2.2  


CONVERGED!
>5LJ5-s nrPDB
          Length = 175

 Score =  347 bits (889), Expect = 2e-96,   Method: Composition-based stats.
 Identities = 175/175 (100%), Positives = 175/175 (100%)

Query: 1   MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNA 60
           MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNA
Sbjct: 1   MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNA 60

Query: 61  LYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSY 120
           LYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSY
Sbjct: 61  LYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSY 120

Query: 121 LKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL 175
           LKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL
Sbjct: 121 LKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL 175


>5ZHX-e nrPDB
          Length = 198

 Score = 29.6 bits (65), Expect = 0.70,   Method: Composition-based stats.
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 21  DQLVKKEFANIKREPVHPEIRGILAKRKGA 50
           D+  ++E A +K+EPV PE    +A R GA
Sbjct: 129 DEHTRRELAKMKQEPVKPEEGRDMANRIGA 158


>5JCP-B nrPDB
          Length = 419

 Score = 29.6 bits (65), Expect = 0.70,   Method: Composition-based stats.
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 21  DQLVKKEFANIKREPVHPEIRGILAKRKGA 50
           D+  ++E A +K+EPV PE    +A R GA
Sbjct: 362 DEHTRRELAKMKQEPVKPEEGRDMANRIGA 391


>5C2K-A nrPDB
          Length = 415

 Score = 29.6 bits (65), Expect = 0.70,   Method: Composition-based stats.
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 21  DQLVKKEFANIKREPVHPEIRGILAKRKGA 50
           D+  ++E A +K+EPV PE    +A R GA
Sbjct: 131 DEHTRRELAKMKQEPVKPEEGRDMANRIGA 160


>3WXM-B nrPDB
          Length = 376

 Score = 28.5 bits (62), Expect = 1.8,   Method: Composition-based stats.
 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 6/46 (13%)

Query: 88  LEEYRRKYPRI-DTSRYIPNESSEVSLLGIVDSYLKHQEIVLDTLL 132
           LEE+ R+  +  DT  Y P E   V+ +G VD+ L     ++DTLL
Sbjct: 277 LEEFLRRIAKSRDTVAYTPGEVLAVARMGAVDTVL-----LVDTLL 317


>1E3H-A nrPDB
          Length = 757

 Score = 28.1 bits (61), Expect = 2.0,   Method: Composition-based stats.
 Identities = 23/93 (24%), Positives = 38/93 (40%), Gaps = 2/93 (2%)

Query: 43  ILAKRKGADNSVSTLTNALYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSR 102
           +L + +G +  +S    AL    +++R   +++  D     D   L       PR+  S 
Sbjct: 323 LLPEFEGREKEISAAYRALTKSLVRERVIAEKKRIDGRGVTDIRTLAAEVEAIPRVHGSA 382

Query: 103 YIPNESSEVSLLGIVDSYLKHQEIVLDTLLPQT 135
               E  E  +LG+    +   E  LDTL P T
Sbjct: 383 LF--ERGETQILGVTTLNMLRMEQQLDTLSPVT 413


>4F7K-A nrPDB
          Length = 425

 Score = 28.1 bits (61), Expect = 2.2,   Method: Composition-based stats.
 Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 3/45 (6%)

Query: 1   MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKR---EPVHPEIRG 42
           +D     + G + DG++N  DQ  +    N  R   EPV P  RG
Sbjct: 137 IDDWRITENGVLADGFENMRDQAHQGRLGNFARALVEPVTPVRRG 181


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.316    0.136    0.383 

Lambda     K      H
   0.267   0.0421    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,056,175
Number of Sequences: 36641
Number of extensions: 281562
Number of successful extensions: 786
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 778
Number of HSP's gapped (non-prelim): 20
length of query: 175
length of database: 10,336,785
effective HSP length: 90
effective length of query: 85
effective length of database: 7,039,095
effective search space: 598323075
effective search space used: 598323075
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.1 bits)