BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q05900 (231 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6G90-C nrPDB 480 e-136 6QX9-1C nrPDB 53 1e-07 2VRD-A nrPDB 52 2e-07 3CW1-L nrPDB 51 4e-07 2CBF-A nrPDB 29 1.5 1CBF-A nrPDB 29 1.7 3GIX-A nrPDB 29 1.7 3VM5-A nrPDB 28 2.6 4DGW-A nrPDB 28 3.0 5NRL-T nrPDB 28 3.2 >6G90-C nrPDB Length = 231 Score = 480 bits (1236), Expect = e-136, Method: Compositional matrix adjust. Identities = 231/231 (100%), Positives = 231/231 (100%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRG 60 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRG Sbjct: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRG 60 Query: 61 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYDGSPGYSKVFV 120 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYDGSPGYSKVFV Sbjct: 61 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYDGSPGYSKVFV 120 Query: 121 DANRFDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKIL 180 DANRFDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKIL Sbjct: 121 DANRFDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKIL 180 Query: 181 SQWSNTIPKTSIFYSVDILQTTIKESKKRMHSDGIRKPSSANGYKRRRYGN 231 SQWSNTIPKTSIFYSVDILQTTIKESKKRMHSDGIRKPSSANGYKRRRYGN Sbjct: 181 SQWSNTIPKTSIFYSVDILQTTIKESKKRMHSDGIRKPSSANGYKRRRYGN 231 >6QX9-1C nrPDB Length = 159 Score = 53.1 bits (126), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 23/52 (44%), Positives = 35/52 (67%), Gaps = 4/52 (7%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYR----NKARDIINK 48 M ++YC+YC +YLTHD+ SVRK+H G+ H DYY+ +A+ +I+K Sbjct: 1 MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYQKWMEEQAQSLIDK 52 >2VRD-A nrPDB Length = 61 Score = 52.0 bits (123), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 23/52 (44%), Positives = 35/52 (67%), Gaps = 4/52 (7%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYR----NKARDIINK 48 M ++YC+YC +YLTHD+ SVRK+H G+ H DYY+ +A+ +I+K Sbjct: 1 MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYQKWMEEQAQSLIDK 52 >3CW1-L nrPDB Length = 77 Score = 51.2 bits (121), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 23/52 (44%), Positives = 34/52 (65%), Gaps = 4/52 (7%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYY----RNKARDIINK 48 M ++YC+YC +YLTHD+ SVRK+H G+ H DYY +A+ +I+K Sbjct: 1 MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYCKWMEEQAQSLIDK 52 >2CBF-A nrPDB Length = 234 Score = 29.3 bits (64), Expect = 1.5, Method: Compositional matrix adjust. Identities = 29/113 (25%), Positives = 47/113 (41%), Gaps = 9/113 (7%) Query: 113 PGYSKVFVDANR----FDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRL 168 PG + VF A I DL + L RA ++ F++ + C F Sbjct: 111 PGVTSVFAAAAAAEAELTIPDLTQTVIL-TRAEGRTPVPEFEKLTDLAKHKCTIALFLSS 169 Query: 169 NNPKKLEPPKILSQWSNTIPKTSIFYSV----DILQTTIKESKKRMHSDGIRK 217 KK+ I + WS P ++ + I++TT+K+ M ++GIRK Sbjct: 170 TLTKKVMKEFINAGWSEDTPVVVVYKATWPDEKIVRTTVKDLDDAMRTNGIRK 222 >1CBF-A nrPDB Length = 285 Score = 29.3 bits (64), Expect = 1.7, Method: Compositional matrix adjust. Identities = 29/113 (25%), Positives = 47/113 (41%), Gaps = 9/113 (7%) Query: 113 PGYSKVFVDANR----FDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRL 168 PG + VF A I DL + L RA ++ F++ + C F Sbjct: 128 PGVTSVFAAAAAAEAELTIPDLTQTVIL-TRAEGRTPVPEFEKLTDLAKHKCTIALFLSS 186 Query: 169 NNPKKLEPPKILSQWSNTIPKTSIFYSV----DILQTTIKESKKRMHSDGIRK 217 KK+ I + WS P ++ + I++TT+K+ M ++GIRK Sbjct: 187 TLTKKVMKEFINAGWSEDTPVVVVYKATWPDEKIVRTTVKDLDDAMRTNGIRK 239 >3GIX-A nrPDB Length = 149 Score = 28.9 bits (63), Expect = 1.7, Method: Compositional matrix adjust. Identities = 20/68 (29%), Positives = 33/68 (48%), Gaps = 4/68 (5%) Query: 136 LPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKILSQWSNTIPKTSIFYS 195 LP+ ++K + K T+ E F R +P L+ ILS+ S+ + K + Y Sbjct: 5 LPKLTSKKEVDQAIKSTA----EKVLVLRFGRDEDPVCLQLDDILSKTSSDLSKMAAIYL 60 Query: 196 VDILQTTI 203 VD+ QT + Sbjct: 61 VDVDQTAV 68 >3VM5-A nrPDB Length = 505 Score = 28.5 bits (62), Expect = 2.6, Method: Compositional matrix adjust. Identities = 14/33 (42%), Positives = 16/33 (48%) Query: 16 DTLSVRKSHLVGKNHLRITADYYRNKARDIINK 48 D VR LVG L + DY R K D +NK Sbjct: 153 DPYQVRDCRLVGLLDLALEKDYVRGKVADFMNK 185 >4DGW-A nrPDB Length = 402 Score = 28.1 bits (61), Expect = 3.0, Method: Compositional matrix adjust. Identities = 13/26 (50%), Positives = 16/26 (61%), Gaps = 2/26 (7%) Query: 5 YCEYCHSYLTHDTLSVRKSHLVGKNH 30 YC +C + T SV +SHLVGK H Sbjct: 294 YCPFCSRWFK--TSSVFESHLVGKIH 317 >5NRL-T nrPDB Length = 530 Score = 28.1 bits (61), Expect = 3.2, Method: Compositional matrix adjust. Identities = 13/26 (50%), Positives = 16/26 (61%), Gaps = 2/26 (7%) Query: 5 YCEYCHSYLTHDTLSVRKSHLVGKNH 30 YC +C + T SV +SHLVGK H Sbjct: 281 YCPFCSRWFK--TSSVFESHLVGKIH 304 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6G90-C nrPDB 422 e-119 6QX9-1C nrPDB 91 4e-19 3CW1-L nrPDB 91 6e-19 2VRD-A nrPDB 86 1e-17 Sequences not found previously or not previously below threshold: 6AHD-X nrPDB 39 0.003 3B6U-A nrPDB 31 0.38 2CBF-A nrPDB 30 0.66 1CBF-A nrPDB 30 0.67 4QTS-C nrPDB 30 0.72 3GIX-A nrPDB 30 1.2 3JCT-I nrPDB 29 2.4 5NRL-T nrPDB 29 2.8 4DGW-A nrPDB 28 3.3 4A9A-A nrPDB 28 4.4 CONVERGED! >6G90-C nrPDB Length = 231 Score = 422 bits (1086), Expect = e-119, Method: Composition-based stats. Identities = 231/231 (100%), Positives = 231/231 (100%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRG 60 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRG Sbjct: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRG 60 Query: 61 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYDGSPGYSKVFV 120 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYDGSPGYSKVFV Sbjct: 61 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYDGSPGYSKVFV 120 Query: 121 DANRFDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKIL 180 DANRFDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKIL Sbjct: 121 DANRFDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKIL 180 Query: 181 SQWSNTIPKTSIFYSVDILQTTIKESKKRMHSDGIRKPSSANGYKRRRYGN 231 SQWSNTIPKTSIFYSVDILQTTIKESKKRMHSDGIRKPSSANGYKRRRYGN Sbjct: 181 SQWSNTIPKTSIFYSVDILQTTIKESKKRMHSDGIRKPSSANGYKRRRYGN 231 >6QX9-1C nrPDB Length = 159 Score = 90.9 bits (224), Expect = 4e-19, Method: Composition-based stats. Identities = 23/52 (44%), Positives = 35/52 (67%), Gaps = 4/52 (7%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYR----NKARDIINK 48 M ++YC+YC +YLTHD+ SVRK+H G+ H DYY+ +A+ +I+K Sbjct: 1 MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYQKWMEEQAQSLIDK 52 >3CW1-L nrPDB Length = 77 Score = 90.5 bits (223), Expect = 6e-19, Method: Composition-based stats. Identities = 23/52 (44%), Positives = 34/52 (65%), Gaps = 4/52 (7%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYY----RNKARDIINK 48 M ++YC+YC +YLTHD+ SVRK+H G+ H DYY +A+ +I+K Sbjct: 1 MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYCKWMEEQAQSLIDK 52 >2VRD-A nrPDB Length = 61 Score = 85.9 bits (211), Expect = 1e-17, Method: Composition-based stats. Identities = 23/52 (44%), Positives = 35/52 (67%), Gaps = 4/52 (7%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYR----NKARDIINK 48 M ++YC+YC +YLTHD+ SVRK+H G+ H DYY+ +A+ +I+K Sbjct: 1 MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVKDYYQKWMEEQAQSLIDK 52 >6AHD-X nrPDB Length = 376 Score = 38.5 bits (88), Expect = 0.003, Method: Composition-based stats. Identities = 12/53 (22%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Query: 2 TRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRR 54 + +C+YC ++ + SV + H GKNH A + ++K + + Sbjct: 9 PKKFCDYCKCWIADNRPSV-EFHERGKNHKENVAKRISEIKQKSLDKAKEEEK 60 >3B6U-A nrPDB Length = 372 Score = 31.2 bits (69), Expect = 0.38, Method: Composition-based stats. Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 11/87 (12%) Query: 10 HSYLTHDTLSVRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIGKRGRKERENSS- 68 TH + S + +LV ++L I Y+ + RD+++K KR + KER ++ Sbjct: 137 DHIFTHISRSQNQQYLVRASYLEI----YQEEIRDLLSKDQTKRLEL-----KERPDTGV 187 Query: 69 -QNETLKVTCLSNKEKRHIMHVKKMNQ 94 + S KE H+M+V N+ Sbjct: 188 YVKDLSSFVTKSVKEIEHVMNVGNQNR 214 >2CBF-A nrPDB Length = 234 Score = 30.5 bits (67), Expect = 0.66, Method: Composition-based stats. Identities = 29/113 (25%), Positives = 47/113 (41%), Gaps = 9/113 (7%) Query: 113 PGYSKVFVDANR----FDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRL 168 PG + VF A I DL + L RA ++ F++ + C F Sbjct: 111 PGVTSVFAAAAAAEAELTIPDLTQTVIL-TRAEGRTPVPEFEKLTDLAKHKCTIALFLSS 169 Query: 169 NNPKKLEPPKILSQWSNTIPKTSIFYSV----DILQTTIKESKKRMHSDGIRK 217 KK+ I + WS P ++ + I++TT+K+ M ++GIRK Sbjct: 170 TLTKKVMKEFINAGWSEDTPVVVVYKATWPDEKIVRTTVKDLDDAMRTNGIRK 222 >1CBF-A nrPDB Length = 285 Score = 30.5 bits (67), Expect = 0.67, Method: Composition-based stats. Identities = 29/113 (25%), Positives = 47/113 (41%), Gaps = 9/113 (7%) Query: 113 PGYSKVFVDANR----FDIGDLVKASKLPQRANEKSAHHSFKQTSRSRDETCESNPFPRL 168 PG + VF A I DL + L RA ++ F++ + C F Sbjct: 128 PGVTSVFAAAAAAEAELTIPDLTQTVIL-TRAEGRTPVPEFEKLTDLAKHKCTIALFLSS 186 Query: 169 NNPKKLEPPKILSQWSNTIPKTSIFYSV----DILQTTIKESKKRMHSDGIRK 217 KK+ I + WS P ++ + I++TT+K+ M ++GIRK Sbjct: 187 TLTKKVMKEFINAGWSEDTPVVVVYKATWPDEKIVRTTVKDLDDAMRTNGIRK 239 >4QTS-C nrPDB Length = 268 Score = 30.5 bits (67), Expect = 0.72, Method: Composition-based stats. Identities = 37/157 (23%), Positives = 61/157 (38%), Gaps = 31/157 (19%) Query: 6 CEYCHSYLTHDTLS--------VRKSHLVGKNHLRITADYYRNKARDIINKHNHKRRHIG 57 CE C + HD+ + VR ++L + + + DY K + I++ G Sbjct: 106 CEICKIFGPHDSKNIKEPVRVIVRDAYLQPEENKKDY-DYLEIKVENTIDRLKGTTIKGG 164 Query: 58 KRGRKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELAQTSIDTLKLLYD------G 111 R + +V S + + ++ K + KEL + I+ +KLL D G Sbjct: 165 IRNME-----------RVVAGSKFKFEVVFNIYKESDKELIKKFIEGMKLLEDDYLGGSG 213 Query: 112 SPGYSKVFVDANRFDIGDLVKASKLPQRANEKSAHHS 148 S GY K+ +F L+ K NE S S Sbjct: 214 SRGYGKI-----KFRDIKLICKPKEYYEGNENSKKES 245 >3GIX-A nrPDB Length = 149 Score = 29.7 bits (65), Expect = 1.2, Method: Composition-based stats. Identities = 20/68 (29%), Positives = 33/68 (48%), Gaps = 4/68 (5%) Query: 136 LPQRANEKSAHHSFKQTSRSRDETCESNPFPRLNNPKKLEPPKILSQWSNTIPKTSIFYS 195 LP+ ++K + K T+ E F R +P L+ ILS+ S+ + K + Y Sbjct: 5 LPKLTSKKEVDQAIKSTA----EKVLVLRFGRDEDPVCLQLDDILSKTSSDLSKMAAIYL 60 Query: 196 VDILQTTI 203 VD+ QT + Sbjct: 61 VDVDQTAV 68 >3JCT-I nrPDB Length = 166 Score = 28.5 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 2/36 (5%) Query: 1 MTRYYCEYCHSYLTHDTLSVRKSHLVGKNHLRITAD 36 + ++YC +C Y+ +T K+HL GK H R + Sbjct: 46 LGQHYCIHCAKYM--ETAIALKTHLKGKVHKRRVKE 79 >5NRL-T nrPDB Length = 530 Score = 28.5 bits (62), Expect = 2.8, Method: Composition-based stats. Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%) Query: 5 YCEYCHSYLTHDTLSVRKSHLVGKNHLRI 33 YC +C + T SV +SHLVGK H + Sbjct: 281 YCPFCSRWFK--TSSVFESHLVGKIHKKN 307 >4DGW-A nrPDB Length = 402 Score = 28.1 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%) Query: 5 YCEYCHSYLTHDTLSVRKSHLVGKNHLRI 33 YC +C + T SV +SHLVGK H + Sbjct: 294 YCPFCSRWFK--TSSVFESHLVGKIHKKN 320 >4A9A-A nrPDB Length = 376 Score = 27.8 bits (60), Expect = 4.4, Method: Composition-based stats. Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 4/70 (5%) Query: 61 RKERENSSQNETLKVTCLSNKEKRHIMHVKKMNQKELA----QTSIDTLKLLYDGSPGYS 116 +KE+ S T+ +T L N E R +M ++N E+A T D + +L S Y Sbjct: 191 KKEKGGISITNTVPLTHLGNDEIRAVMSEYRINSAEIAFRCDATVDDLIDVLEASSRRYM 250 Query: 117 KVFVDANRFD 126 N+ D Sbjct: 251 PAIYVLNKID 260 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.130 0.375 Lambda K H 0.267 0.0405 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,281,703 Number of Sequences: 36641 Number of extensions: 343202 Number of successful extensions: 831 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 813 Number of HSP's gapped (non-prelim): 38 length of query: 231 length of database: 10,336,785 effective HSP length: 94 effective length of query: 137 effective length of database: 6,892,531 effective search space: 944276747 effective search space used: 944276747 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 57 (26.6 bits)