BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q05789 (226 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5KC1-C nrPDB 451 e-128 4H03-A nrPDB 29 1.4 5X2G-A nrPDB 28 3.5 4B3K-A nrPDB 27 5.1 4O8Y-B nrPDB 27 6.1 5MPD-V nrPDB 27 6.3 6DXW-A nrPDB 27 9.1 1QVR-A nrPDB 27 9.6 >5KC1-C nrPDB Length = 226 Score = 451 bits (1161), Expect = e-128, Method: Compositional matrix adjust. Identities = 226/226 (100%), Positives = 226/226 (100%) Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL Sbjct: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60 Query: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ Sbjct: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120 Query: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK Sbjct: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180 Query: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN Sbjct: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226 >4H03-A nrPDB Length = 418 Score = 29.3 bits (64), Expect = 1.4, Method: Compositional matrix adjust. Identities = 44/184 (23%), Positives = 84/184 (45%), Gaps = 22/184 (11%) Query: 39 PQNENLSQNKLSSDVTQAIDLLKQDITAKIQEL-ELLIEKQSSEENNIGMVNNNMLIGSV 97 P+N + S+DV L++QD + KI ++ ++IE + + +VN+ V Sbjct: 166 PKNTGMLPYINSNDVKT---LIEQDYSIKIDKIVRIVIEGKQYIKAEASIVNSLDFKDDV 222 Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157 +K + G N +W N + L+ +ND + R + N+ L + G N+ Sbjct: 223 ---SKGDLWGKENYSDWSNKLTPNELADVNDYM------RGGYTAINNY-LISNGPLNNP 272 Query: 158 NSEM--KIN-----LRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERW 210 N E+ K+N L+L L++Y + +E+G+ + + K+ N I +E+W Sbjct: 273 NPELDSKVNNIENALKLTPIPSNLIVYRRSGPQEFGLTLTSPEYDFNKIEN-IDAFKEKW 331 Query: 211 DSLV 214 + V Sbjct: 332 EGKV 335 >5X2G-A nrPDB Length = 835 Score = 28.1 bits (61), Expect = 3.5, Method: Compositional matrix adjust. Identities = 20/56 (35%), Positives = 32/56 (57%), Gaps = 3/56 (5%) Query: 12 EDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLLKQDITAK 67 +D E + KG + KY+E I+ LG NLSQ+ L +++ + I L+K +I K Sbjct: 323 DDYEFKGEKGTYFIEFKKYKEFIKALGEH--NLSQDDL-NEIAKDITLIKDEIKLK 375 >4B3K-A nrPDB Length = 479 Score = 27.3 bits (59), Expect = 5.1, Method: Compositional matrix adjust. Identities = 15/71 (21%), Positives = 32/71 (45%) Query: 35 EVLGPQNENLSQNKLSSDVTQAIDLLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLI 94 + +GP + + +++ SD+T L IQ L+ + + + N G+ N +I Sbjct: 43 DYVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRVI 102 Query: 95 GSVILNNKSPI 105 + + N P+ Sbjct: 103 DACLANGIRPV 113 >4O8Y-B nrPDB Length = 240 Score = 27.3 bits (59), Expect = 6.1, Method: Compositional matrix adjust. Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 6/48 (12%) Query: 131 LMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYE 178 L+ LNR ++LN D K +K ++M +NL EQ++ L +Y+ Sbjct: 189 LIHGLNRHYYSLNIDYH------KTAKETKMLMNLHKEQWQSGLKMYD 230 >5MPD-V nrPDB Length = 306 Score = 26.9 bits (58), Expect = 6.3, Method: Compositional matrix adjust. Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 6/48 (12%) Query: 131 LMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYE 178 L+ LNR ++LN D K +K ++M +NL EQ++ L +Y+ Sbjct: 188 LIHGLNRHYYSLNIDYH------KTAKETKMLMNLHKEQWQSGLKMYD 229 >6DXW-A nrPDB Length = 341 Score = 26.6 bits (57), Expect = 9.1, Method: Compositional matrix adjust. Identities = 21/64 (32%), Positives = 27/64 (42%), Gaps = 6/64 (9%) Query: 70 ELELLIEKQSSEENN----IGMVNNNMLIGSV--ILNNKSPINGISNARNWDNPAYQDTL 123 + LI SE N +G + LI V I+ SP G+ RN D PA L Sbjct: 197 PVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPL 256 Query: 124 SPIN 127 P+N Sbjct: 257 DPLN 260 >1QVR-A nrPDB Length = 854 Score = 26.6 bits (57), Expect = 9.6, Method: Composition-based stats. Identities = 18/68 (26%), Positives = 31/68 (45%), Gaps = 7/68 (10%) Query: 157 KNSEMKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERWDSLVES 216 + ++++ + E KKE Q++ K I E KL EI +LR W+ E Sbjct: 407 ERKKLQLEIEREALKKEKDPDSQERLKA-------IEAEIAKLTEEIAKLRAEWEREREI 459 Query: 217 AKQRRDKQ 224 ++ R+ Q Sbjct: 460 LRKLREAQ 467 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5KC1-C nrPDB 405 e-114 Sequences not found previously or not previously below threshold: 4H03-A nrPDB 37 0.008 5MPD-V nrPDB 33 0.13 5WJ7-A nrPDB 33 0.13 4O8Y-B nrPDB 32 0.23 2QF4-A nrPDB 32 0.25 3HH0-A nrPDB 31 0.45 5OQP-A nrPDB 31 0.53 6B3O-A nrPDB 30 0.65 4L6H-A nrPDB 30 0.81 5O09-1C nrPDB 29 1.7 4NX9-A nrPDB 29 2.0 6DXW-A nrPDB 28 3.1 2AO9-A nrPDB 27 5.9 5NGM-Af nrPDB 27 6.7 CONVERGED! >5KC1-C nrPDB Length = 226 Score = 405 bits (1041), Expect = e-114, Method: Composition-based stats. Identities = 226/226 (100%), Positives = 226/226 (100%) Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL Sbjct: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60 Query: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ Sbjct: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120 Query: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK Sbjct: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180 Query: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN Sbjct: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226 >4H03-A nrPDB Length = 418 Score = 36.6 bits (83), Expect = 0.008, Method: Composition-based stats. Identities = 44/184 (23%), Positives = 84/184 (45%), Gaps = 22/184 (11%) Query: 39 PQNENLSQNKLSSDVTQAIDLLKQDITAKIQEL-ELLIEKQSSEENNIGMVNNNMLIGSV 97 P+N + S+DV L++QD + KI ++ ++IE + + +VN+ V Sbjct: 166 PKNTGMLPYINSNDVKT---LIEQDYSIKIDKIVRIVIEGKQYIKAEASIVNSLDFKDDV 222 Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157 +K + G N +W N + L+ +ND + R + N+ L + G N+ Sbjct: 223 ---SKGDLWGKENYSDWSNKLTPNELADVNDYM------RGGYTAINNY-LISNGPLNNP 272 Query: 158 NSEM--KIN-----LRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERW 210 N E+ K+N L+L L++Y + +E+G+ + + K+ N I +E+W Sbjct: 273 NPELDSKVNNIENALKLTPIPSNLIVYRRSGPQEFGLTLTSPEYDFNKIEN-IDAFKEKW 331 Query: 211 DSLV 214 + V Sbjct: 332 EGKV 335 >5MPD-V nrPDB Length = 306 Score = 32.8 bits (73), Expect = 0.13, Method: Composition-based stats. Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 18/108 (16%) Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157 ++NN P SN L+ N L+ LNR ++LN D K +K Sbjct: 164 LINNLEPRQTTSNT---------GLLNKANIQALIHGLNRHYYSLNIDYH------KTAK 208 Query: 158 NSEMKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGR 205 ++M +NL EQ++ L +Y+ ++ +E + TK K+A + + Sbjct: 209 ETKMLMNLHKEQWQSGLKMYDYEEKEESNLA---ATKSMVKIAEQYSK 253 >5WJ7-A nrPDB Length = 199 Score = 32.8 bits (73), Expect = 0.13, Method: Composition-based stats. Identities = 19/72 (26%), Positives = 40/72 (55%), Gaps = 8/72 (11%) Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60 + L+++ T +E+A QECR NA+ K ++AI E L + + D + ++ + Sbjct: 133 LDDLSQLQTEVEEAVQECR-----NAEEKAKKAITDAAMMAEELKKEQ---DTSAHLERM 184 Query: 61 KQDITAKIQELE 72 K+++ I++L+ Sbjct: 185 KKNMEQTIKDLQ 196 >4O8Y-B nrPDB Length = 240 Score = 32.0 bits (71), Expect = 0.23, Method: Composition-based stats. Identities = 24/87 (27%), Positives = 41/87 (47%), Gaps = 15/87 (17%) Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157 ++NN P SN L+ N L+ LNR ++LN D K +K Sbjct: 165 LINNLEPRQTTSNT---------GLLNKANIQALIHGLNRHYYSLNIDYH------KTAK 209 Query: 158 NSEMKINLRLEQFKKELVLYEQKKFKE 184 ++M +NL EQ++ L +Y+ ++ +E Sbjct: 210 ETKMLMNLHKEQWQSGLKMYDYEEKEE 236 >2QF4-A nrPDB Length = 172 Score = 31.6 bits (70), Expect = 0.25, Method: Composition-based stats. Identities = 21/71 (29%), Positives = 35/71 (49%), Gaps = 2/71 (2%) Query: 47 NKLSSDVTQAIDLLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSV--ILNNKSP 104 +KL + T A D++ + + QEL L + N+ + N LIGSV + N + Sbjct: 1 SKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTI 60 Query: 105 INGISNARNWD 115 +N ++N N D Sbjct: 61 VNLLTNTENAD 71 >3HH0-A nrPDB Length = 146 Score = 30.9 bits (68), Expect = 0.45, Method: Composition-based stats. Identities = 13/42 (30%), Positives = 28/42 (66%) Query: 157 KNSEMKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKK 198 ++ E ++ LR F++E++L EQ++ + +DE+TK+ +K Sbjct: 74 RDIETEVFLRQMHFQREVLLAEQERIAKVLSHMDEMTKKFQK 115 >5OQP-A nrPDB Length = 869 Score = 30.9 bits (68), Expect = 0.53, Method: Composition-based stats. Identities = 23/98 (23%), Positives = 46/98 (46%), Gaps = 9/98 (9%) Query: 6 EVYTIIEDAEQECRKGDFTNAKA-------KYQEAIEVLGPQNENLSQNKLSSDVTQAID 58 ++Y IE ++E +K TN A Y E+L ++ + L + A+D Sbjct: 774 KIYAQIE--KKEIKKMIITNINAIFLSSEQDYSTLKELLEYSDDIAENDNLDNVSKNALD 831 Query: 59 LLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGS 96 L+ ++ + I+E+ E Q+ +ENN + ++G+ Sbjct: 832 KLRNNLNSLIEEINERSETQTKDENNTANDQYSSILGN 869 >6B3O-A nrPDB Length = 605 Score = 30.5 bits (67), Expect = 0.65, Method: Composition-based stats. Identities = 12/29 (41%), Positives = 20/29 (68%) Query: 180 KKFKEYGMKIDEITKENKKLANEIGRLRE 208 K+ K+ KI+EI + KK+ NEI R+++ Sbjct: 559 KRMKQIEDKIEEIESKQKKIENEIARIKK 587 >4L6H-A nrPDB Length = 789 Score = 30.1 bits (66), Expect = 0.81, Method: Composition-based stats. Identities = 32/146 (21%), Positives = 61/146 (41%), Gaps = 30/146 (20%) Query: 4 LAEVYTIIEDAE---------QECRKGDFTNAKAKYQEAIEVLGPQ------NENLSQNK 48 L EV +I++D + + K DF A A Q+AIE +G + +L Sbjct: 306 LDEVASILKDGQTLSAGVVDGRNIWKTDFAKASAVVQKAIEKVGKDKVVVATSSSLLHTP 365 Query: 49 LSSDVTQAIDLLKQD----ITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSP 104 + + +D + +D T K+ E+ ++ + S E+ + + N + Sbjct: 366 VDLESETKLDAVIKDWFSFATQKLDEVVVIAKNVSGED-----------VSKQLEANAAS 414 Query: 105 INGISNARNWDNPAYQDTLSPINDPL 130 I S + ++P Q+ L+ IN+ L Sbjct: 415 IKARSESSITNDPKVQERLTTINEAL 440 >5O09-1C nrPDB Length = 238 Score = 28.9 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 25/98 (25%), Positives = 48/98 (48%), Gaps = 9/98 (9%) Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAI---EVLGPQNENLSQNKLSSDVTQAI 57 + L E +++ D Q ++GDF A++ Y+EA+ + L Q+ +L + S + Sbjct: 29 VGALVEQISVLADLRQ--KEGDFRKAESLYREALFRAQELRKQDPDLLTG-IYSLLAHLY 85 Query: 58 DLL-KQDITAKIQELELLIEKQS--SEENNIGMVNNNM 92 D + D A+ EL L I ++ E + + + NN+ Sbjct: 86 DRWGRMDKAAEFYELALKISAENGLEESDKVATIKNNL 123 >4NX9-A nrPDB Length = 293 Score = 28.9 bits (63), Expect = 2.0, Method: Composition-based stats. Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 15/73 (20%) Query: 102 KSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEM 161 K+ +GIS A+ + Q T +IL R++ D+ L++ G NS + Sbjct: 17 KNANDGISLAQTAEGALQQST----------NILQRMR-----DLSLQSANGSNSDSERT 61 Query: 162 KINLRLEQFKKEL 174 +N ++Q +KEL Sbjct: 62 ALNGEVKQLQKEL 74 >6DXW-A nrPDB Length = 341 Score = 28.2 bits (61), Expect = 3.1, Method: Composition-based stats. Identities = 21/60 (35%), Positives = 26/60 (43%), Gaps = 6/60 (10%) Query: 74 LIEKQSSEENN----IGMVNNNMLIGSV--ILNNKSPINGISNARNWDNPAYQDTLSPIN 127 LI SE N +G + LI V I+ SP G+ RN D PA L P+N Sbjct: 201 LIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLN 260 >2AO9-A nrPDB Length = 155 Score = 27.0 bits (58), Expect = 5.9, Method: Composition-based stats. Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 1/35 (2%) Query: 51 SDVTQAIDLLKQDITAK-IQELELLIEKQSSEENN 84 S++ +D LKQ +TAK IQ LL+E + E NN Sbjct: 11 SEMMAKLDELKQKLTAKQIQAAYLLVENELMESNN 45 >5NGM-Af nrPDB Length = 98 Score = 27.0 bits (58), Expect = 6.7, Method: Composition-based stats. Identities = 16/61 (26%), Positives = 27/61 (44%) Query: 165 LRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQ 224 L + + K + YE FK+ I + +N K +E RL + D ++ R D+ Sbjct: 38 LEAKDWGKRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDED 97 Query: 225 K 225 K Sbjct: 98 K 98 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.128 0.341 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,816,721 Number of Sequences: 36641 Number of extensions: 294943 Number of successful extensions: 889 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 36 Number of HSP's that attempted gapping in prelim test: 857 Number of HSP's gapped (non-prelim): 63 length of query: 226 length of database: 10,336,785 effective HSP length: 94 effective length of query: 132 effective length of database: 6,892,531 effective search space: 909814092 effective search space used: 909814092 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 57 (26.6 bits)