BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= Q05789
(226 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5KC1-C nrPDB 451 e-128
4H03-A nrPDB 29 1.4
5X2G-A nrPDB 28 3.5
4B3K-A nrPDB 27 5.1
4O8Y-B nrPDB 27 6.1
5MPD-V nrPDB 27 6.3
6DXW-A nrPDB 27 9.1
1QVR-A nrPDB 27 9.6
>5KC1-C nrPDB
Length = 226
Score = 451 bits (1161), Expect = e-128, Method: Compositional matrix adjust.
Identities = 226/226 (100%), Positives = 226/226 (100%)
Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60
MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL
Sbjct: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60
Query: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120
KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ
Sbjct: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120
Query: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180
DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK
Sbjct: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180
Query: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226
KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN
Sbjct: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226
>4H03-A nrPDB
Length = 418
Score = 29.3 bits (64), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 44/184 (23%), Positives = 84/184 (45%), Gaps = 22/184 (11%)
Query: 39 PQNENLSQNKLSSDVTQAIDLLKQDITAKIQEL-ELLIEKQSSEENNIGMVNNNMLIGSV 97
P+N + S+DV L++QD + KI ++ ++IE + + +VN+ V
Sbjct: 166 PKNTGMLPYINSNDVKT---LIEQDYSIKIDKIVRIVIEGKQYIKAEASIVNSLDFKDDV 222
Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157
+K + G N +W N + L+ +ND + R + N+ L + G N+
Sbjct: 223 ---SKGDLWGKENYSDWSNKLTPNELADVNDYM------RGGYTAINNY-LISNGPLNNP 272
Query: 158 NSEM--KIN-----LRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERW 210
N E+ K+N L+L L++Y + +E+G+ + + K+ N I +E+W
Sbjct: 273 NPELDSKVNNIENALKLTPIPSNLIVYRRSGPQEFGLTLTSPEYDFNKIEN-IDAFKEKW 331
Query: 211 DSLV 214
+ V
Sbjct: 332 EGKV 335
>5X2G-A nrPDB
Length = 835
Score = 28.1 bits (61), Expect = 3.5, Method: Compositional matrix adjust.
Identities = 20/56 (35%), Positives = 32/56 (57%), Gaps = 3/56 (5%)
Query: 12 EDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLLKQDITAK 67
+D E + KG + KY+E I+ LG NLSQ+ L +++ + I L+K +I K
Sbjct: 323 DDYEFKGEKGTYFIEFKKYKEFIKALGEH--NLSQDDL-NEIAKDITLIKDEIKLK 375
>4B3K-A nrPDB
Length = 479
Score = 27.3 bits (59), Expect = 5.1, Method: Compositional matrix adjust.
Identities = 15/71 (21%), Positives = 32/71 (45%)
Query: 35 EVLGPQNENLSQNKLSSDVTQAIDLLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLI 94
+ +GP + + +++ SD+T L IQ L+ + + + N G+ N +I
Sbjct: 43 DYVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRVI 102
Query: 95 GSVILNNKSPI 105
+ + N P+
Sbjct: 103 DACLANGIRPV 113
>4O8Y-B nrPDB
Length = 240
Score = 27.3 bits (59), Expect = 6.1, Method: Compositional matrix adjust.
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 6/48 (12%)
Query: 131 LMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYE 178
L+ LNR ++LN D K +K ++M +NL EQ++ L +Y+
Sbjct: 189 LIHGLNRHYYSLNIDYH------KTAKETKMLMNLHKEQWQSGLKMYD 230
>5MPD-V nrPDB
Length = 306
Score = 26.9 bits (58), Expect = 6.3, Method: Compositional matrix adjust.
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 6/48 (12%)
Query: 131 LMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYE 178
L+ LNR ++LN D K +K ++M +NL EQ++ L +Y+
Sbjct: 188 LIHGLNRHYYSLNIDYH------KTAKETKMLMNLHKEQWQSGLKMYD 229
>6DXW-A nrPDB
Length = 341
Score = 26.6 bits (57), Expect = 9.1, Method: Compositional matrix adjust.
Identities = 21/64 (32%), Positives = 27/64 (42%), Gaps = 6/64 (9%)
Query: 70 ELELLIEKQSSEENN----IGMVNNNMLIGSV--ILNNKSPINGISNARNWDNPAYQDTL 123
+ LI SE N +G + LI V I+ SP G+ RN D PA L
Sbjct: 197 PVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPL 256
Query: 124 SPIN 127
P+N
Sbjct: 257 DPLN 260
>1QVR-A nrPDB
Length = 854
Score = 26.6 bits (57), Expect = 9.6, Method: Composition-based stats.
Identities = 18/68 (26%), Positives = 31/68 (45%), Gaps = 7/68 (10%)
Query: 157 KNSEMKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERWDSLVES 216
+ ++++ + E KKE Q++ K I E KL EI +LR W+ E
Sbjct: 407 ERKKLQLEIEREALKKEKDPDSQERLKA-------IEAEIAKLTEEIAKLRAEWEREREI 459
Query: 217 AKQRRDKQ 224
++ R+ Q
Sbjct: 460 LRKLREAQ 467
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5KC1-C nrPDB 405 e-114
Sequences not found previously or not previously below threshold:
4H03-A nrPDB 37 0.008
5MPD-V nrPDB 33 0.13
5WJ7-A nrPDB 33 0.13
4O8Y-B nrPDB 32 0.23
2QF4-A nrPDB 32 0.25
3HH0-A nrPDB 31 0.45
5OQP-A nrPDB 31 0.53
6B3O-A nrPDB 30 0.65
4L6H-A nrPDB 30 0.81
5O09-1C nrPDB 29 1.7
4NX9-A nrPDB 29 2.0
6DXW-A nrPDB 28 3.1
2AO9-A nrPDB 27 5.9
5NGM-Af nrPDB 27 6.7
CONVERGED!
>5KC1-C nrPDB
Length = 226
Score = 405 bits (1041), Expect = e-114, Method: Composition-based stats.
Identities = 226/226 (100%), Positives = 226/226 (100%)
Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60
MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL
Sbjct: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60
Query: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120
KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ
Sbjct: 61 KQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSPINGISNARNWDNPAYQ 120
Query: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180
DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK
Sbjct: 121 DTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEMKINLRLEQFKKELVLYEQK 180
Query: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226
KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN
Sbjct: 181 KFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQKN 226
>4H03-A nrPDB
Length = 418
Score = 36.6 bits (83), Expect = 0.008, Method: Composition-based stats.
Identities = 44/184 (23%), Positives = 84/184 (45%), Gaps = 22/184 (11%)
Query: 39 PQNENLSQNKLSSDVTQAIDLLKQDITAKIQEL-ELLIEKQSSEENNIGMVNNNMLIGSV 97
P+N + S+DV L++QD + KI ++ ++IE + + +VN+ V
Sbjct: 166 PKNTGMLPYINSNDVKT---LIEQDYSIKIDKIVRIVIEGKQYIKAEASIVNSLDFKDDV 222
Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157
+K + G N +W N + L+ +ND + R + N+ L + G N+
Sbjct: 223 ---SKGDLWGKENYSDWSNKLTPNELADVNDYM------RGGYTAINNY-LISNGPLNNP 272
Query: 158 NSEM--KIN-----LRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERW 210
N E+ K+N L+L L++Y + +E+G+ + + K+ N I +E+W
Sbjct: 273 NPELDSKVNNIENALKLTPIPSNLIVYRRSGPQEFGLTLTSPEYDFNKIEN-IDAFKEKW 331
Query: 211 DSLV 214
+ V
Sbjct: 332 EGKV 335
>5MPD-V nrPDB
Length = 306
Score = 32.8 bits (73), Expect = 0.13, Method: Composition-based stats.
Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 18/108 (16%)
Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157
++NN P SN L+ N L+ LNR ++LN D K +K
Sbjct: 164 LINNLEPRQTTSNT---------GLLNKANIQALIHGLNRHYYSLNIDYH------KTAK 208
Query: 158 NSEMKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGR 205
++M +NL EQ++ L +Y+ ++ +E + TK K+A + +
Sbjct: 209 ETKMLMNLHKEQWQSGLKMYDYEEKEESNLA---ATKSMVKIAEQYSK 253
>5WJ7-A nrPDB
Length = 199
Score = 32.8 bits (73), Expect = 0.13, Method: Composition-based stats.
Identities = 19/72 (26%), Positives = 40/72 (55%), Gaps = 8/72 (11%)
Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENLSQNKLSSDVTQAIDLL 60
+ L+++ T +E+A QECR NA+ K ++AI E L + + D + ++ +
Sbjct: 133 LDDLSQLQTEVEEAVQECR-----NAEEKAKKAITDAAMMAEELKKEQ---DTSAHLERM 184
Query: 61 KQDITAKIQELE 72
K+++ I++L+
Sbjct: 185 KKNMEQTIKDLQ 196
>4O8Y-B nrPDB
Length = 240
Score = 32.0 bits (71), Expect = 0.23, Method: Composition-based stats.
Identities = 24/87 (27%), Positives = 41/87 (47%), Gaps = 15/87 (17%)
Query: 98 ILNNKSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSK 157
++NN P SN L+ N L+ LNR ++LN D K +K
Sbjct: 165 LINNLEPRQTTSNT---------GLLNKANIQALIHGLNRHYYSLNIDYH------KTAK 209
Query: 158 NSEMKINLRLEQFKKELVLYEQKKFKE 184
++M +NL EQ++ L +Y+ ++ +E
Sbjct: 210 ETKMLMNLHKEQWQSGLKMYDYEEKEE 236
>2QF4-A nrPDB
Length = 172
Score = 31.6 bits (70), Expect = 0.25, Method: Composition-based stats.
Identities = 21/71 (29%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
Query: 47 NKLSSDVTQAIDLLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSV--ILNNKSP 104
+KL + T A D++ + + QEL L + N+ + N LIGSV + N +
Sbjct: 1 SKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTI 60
Query: 105 INGISNARNWD 115
+N ++N N D
Sbjct: 61 VNLLTNTENAD 71
>3HH0-A nrPDB
Length = 146
Score = 30.9 bits (68), Expect = 0.45, Method: Composition-based stats.
Identities = 13/42 (30%), Positives = 28/42 (66%)
Query: 157 KNSEMKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKK 198
++ E ++ LR F++E++L EQ++ + +DE+TK+ +K
Sbjct: 74 RDIETEVFLRQMHFQREVLLAEQERIAKVLSHMDEMTKKFQK 115
>5OQP-A nrPDB
Length = 869
Score = 30.9 bits (68), Expect = 0.53, Method: Composition-based stats.
Identities = 23/98 (23%), Positives = 46/98 (46%), Gaps = 9/98 (9%)
Query: 6 EVYTIIEDAEQECRKGDFTNAKA-------KYQEAIEVLGPQNENLSQNKLSSDVTQAID 58
++Y IE ++E +K TN A Y E+L ++ + L + A+D
Sbjct: 774 KIYAQIE--KKEIKKMIITNINAIFLSSEQDYSTLKELLEYSDDIAENDNLDNVSKNALD 831
Query: 59 LLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGS 96
L+ ++ + I+E+ E Q+ +ENN + ++G+
Sbjct: 832 KLRNNLNSLIEEINERSETQTKDENNTANDQYSSILGN 869
>6B3O-A nrPDB
Length = 605
Score = 30.5 bits (67), Expect = 0.65, Method: Composition-based stats.
Identities = 12/29 (41%), Positives = 20/29 (68%)
Query: 180 KKFKEYGMKIDEITKENKKLANEIGRLRE 208
K+ K+ KI+EI + KK+ NEI R+++
Sbjct: 559 KRMKQIEDKIEEIESKQKKIENEIARIKK 587
>4L6H-A nrPDB
Length = 789
Score = 30.1 bits (66), Expect = 0.81, Method: Composition-based stats.
Identities = 32/146 (21%), Positives = 61/146 (41%), Gaps = 30/146 (20%)
Query: 4 LAEVYTIIEDAE---------QECRKGDFTNAKAKYQEAIEVLGPQ------NENLSQNK 48
L EV +I++D + + K DF A A Q+AIE +G + +L
Sbjct: 306 LDEVASILKDGQTLSAGVVDGRNIWKTDFAKASAVVQKAIEKVGKDKVVVATSSSLLHTP 365
Query: 49 LSSDVTQAIDLLKQD----ITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNKSP 104
+ + +D + +D T K+ E+ ++ + S E+ + + N +
Sbjct: 366 VDLESETKLDAVIKDWFSFATQKLDEVVVIAKNVSGED-----------VSKQLEANAAS 414
Query: 105 INGISNARNWDNPAYQDTLSPINDPL 130
I S + ++P Q+ L+ IN+ L
Sbjct: 415 IKARSESSITNDPKVQERLTTINEAL 440
>5O09-1C nrPDB
Length = 238
Score = 28.9 bits (63), Expect = 1.7, Method: Composition-based stats.
Identities = 25/98 (25%), Positives = 48/98 (48%), Gaps = 9/98 (9%)
Query: 1 MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAI---EVLGPQNENLSQNKLSSDVTQAI 57
+ L E +++ D Q ++GDF A++ Y+EA+ + L Q+ +L + S +
Sbjct: 29 VGALVEQISVLADLRQ--KEGDFRKAESLYREALFRAQELRKQDPDLLTG-IYSLLAHLY 85
Query: 58 DLL-KQDITAKIQELELLIEKQS--SEENNIGMVNNNM 92
D + D A+ EL L I ++ E + + + NN+
Sbjct: 86 DRWGRMDKAAEFYELALKISAENGLEESDKVATIKNNL 123
>4NX9-A nrPDB
Length = 293
Score = 28.9 bits (63), Expect = 2.0, Method: Composition-based stats.
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 15/73 (20%)
Query: 102 KSPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSEM 161
K+ +GIS A+ + Q T +IL R++ D+ L++ G NS +
Sbjct: 17 KNANDGISLAQTAEGALQQST----------NILQRMR-----DLSLQSANGSNSDSERT 61
Query: 162 KINLRLEQFKKEL 174
+N ++Q +KEL
Sbjct: 62 ALNGEVKQLQKEL 74
>6DXW-A nrPDB
Length = 341
Score = 28.2 bits (61), Expect = 3.1, Method: Composition-based stats.
Identities = 21/60 (35%), Positives = 26/60 (43%), Gaps = 6/60 (10%)
Query: 74 LIEKQSSEENN----IGMVNNNMLIGSV--ILNNKSPINGISNARNWDNPAYQDTLSPIN 127
LI SE N +G + LI V I+ SP G+ RN D PA L P+N
Sbjct: 201 LIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLN 260
>2AO9-A nrPDB
Length = 155
Score = 27.0 bits (58), Expect = 5.9, Method: Composition-based stats.
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Query: 51 SDVTQAIDLLKQDITAK-IQELELLIEKQSSEENN 84
S++ +D LKQ +TAK IQ LL+E + E NN
Sbjct: 11 SEMMAKLDELKQKLTAKQIQAAYLLVENELMESNN 45
>5NGM-Af nrPDB
Length = 98
Score = 27.0 bits (58), Expect = 6.7, Method: Composition-based stats.
Identities = 16/61 (26%), Positives = 27/61 (44%)
Query: 165 LRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAKQRRDKQ 224
L + + K + YE FK+ I + +N K +E RL + D ++ R D+
Sbjct: 38 LEAKDWGKRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDED 97
Query: 225 K 225
K
Sbjct: 98 K 98
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.309 0.128 0.341
Lambda K H
0.267 0.0400 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,816,721
Number of Sequences: 36641
Number of extensions: 294943
Number of successful extensions: 889
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 36
Number of HSP's that attempted gapping in prelim test: 857
Number of HSP's gapped (non-prelim): 63
length of query: 226
length of database: 10,336,785
effective HSP length: 94
effective length of query: 132
effective length of database: 6,892,531
effective search space: 909814092
effective search space used: 909814092
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 57 (26.6 bits)