BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q05776 (175 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5JQM-A nrPDB 364 e-101 4XHR-A nrPDB 363 e-101 6I3V-F nrPDB 100 3e-22 6I4Y-B nrPDB 80 4e-16 4XZV-B nrPDB 74 4e-14 6AXF-A nrPDB 28 2.0 5ZQM-A nrPDB 28 2.1 6B5C-A nrPDB 28 2.6 4ZEN-A nrPDB 27 3.4 5IMW-A nrPDB 27 3.7 6AI8-A nrPDB 27 6.8 >5JQM-A nrPDB Length = 283 Score = 364 bits (935), Expect = e-101, Method: Compositional matrix adjust. Identities = 175/175 (100%), Positives = 175/175 (100%) Query: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG Sbjct: 15 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 74 Query: 61 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS Sbjct: 75 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 134 Query: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 175 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF Sbjct: 135 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 189 >4XHR-A nrPDB Length = 189 Score = 363 bits (932), Expect = e-101, Method: Compositional matrix adjust. Identities = 175/175 (100%), Positives = 175/175 (100%) Query: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG Sbjct: 15 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 74 Query: 61 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS Sbjct: 75 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 134 Query: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 175 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF Sbjct: 135 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 189 >6I3V-F nrPDB Length = 182 Score = 100 bits (250), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 54/170 (31%), Positives = 88/170 (51%), Gaps = 2/170 (1%) Query: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60 MV + + + V AF+ RYPNPYS HVL+ D + R V + L + RLL K+ Sbjct: 10 MVKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLLSRRLLTKTN 69 Query: 61 KLPTWVKPFLRG--ITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSAT 118 ++P W + +++E S+V+P N TM T+T N++H +M VEE + Y +S Sbjct: 70 RMPRWAERLFPANVAHSVYVLEDSIVDPQNQTMTTFTWNINHARLMVVEERSVYSVNSDN 129 Query: 119 SSTIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLE 168 S R + S G+ V+++ +F NV K+ G +++ KL+ Sbjct: 130 SGWTEIRREAWVSSSLFGVSRAVQEFGLARFKSNVTKTMKGFEYILAKLQ 179 >6I4Y-B nrPDB Length = 207 Score = 80.5 bits (197), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 47/162 (29%), Positives = 80/162 (49%), Gaps = 1/162 (0%) Query: 7 STHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSGKLPTWV 66 S H+F + +V+ A +YPNP +P V+ +D + R++D G L + RLL LP+ V Sbjct: 19 SEHVFDHPWETVTTAAMQKYPNPMNPSVVGVDVLDRHIDPSGKLHSHRLLSTEWGLPSIV 78 Query: 67 KPFLRGI-TETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSSTIADS 125 K + T+T++ E SVV+P TM+ + N+ T ++ V+E Y+ + Sbjct: 79 KSLIGAARTKTYVQEHSVVDPVEKTMELKSTNISFTNMVSVDERLIYKPHPQDPEKTVLT 138 Query: 126 RVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKL 167 + + + + S +E + N K R M +VI KL Sbjct: 139 QEAIITVKGVSLSSYLEGLMASTISSNASKGREAMEWVIHKL 180 >4XZV-B nrPDB Length = 186 Score = 73.9 bits (180), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 49/167 (29%), Positives = 79/167 (47%), Gaps = 5/167 (2%) Query: 7 STHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSGKLPTWV 66 S H+F + +V +A +YPNP +P VL +D + R VD G L + RLL LP+ V Sbjct: 20 SEHVFGHPWDTVIQAAMRKYPNPMNPSVLGVDVLQRRVDGRGRLHSLRLLSTEWGLPSLV 79 Query: 67 KPFL-RGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTY--QFDSATSSTIA 123 + L T T+I E SVV+P M+ + N+ T ++ V E Y ++ + + Sbjct: 80 RAILGTSRTLTYIREHSVVDPVEKKMELCSTNITLTNLVSVNERLVYTPHPENPEMTVLT 139 Query: 124 DSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEA 170 + G ++G S +E N KK + ++I+ E A Sbjct: 140 QEAIITVKGISLG--SYLESLMANTISSNAKKGWAAIEWIIEHSESA 184 >6AXF-A nrPDB Length = 397 Score = 28.1 bits (61), Expect = 2.0, Method: Compositional matrix adjust. Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 11/69 (15%) Query: 44 VDQEGNLRTTRLLKKSGKLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGI 103 +DQEGN R + L+ +PT+ W +V+ NP + + DH Sbjct: 110 LDQEGNRRHSSLIDIDS-VPTY----------KWKRQVTQRNPVGQKKRKMSLLFDHLEP 158 Query: 104 MKVEEYTTY 112 M++ E+ TY Sbjct: 159 MELAEHLTY 167 >5ZQM-A nrPDB Length = 309 Score = 28.1 bits (61), Expect = 2.1, Method: Compositional matrix adjust. Identities = 16/50 (32%), Positives = 23/50 (46%), Gaps = 5/50 (10%) Query: 33 HVLSIDTISRNV-----DQEGNLRTTRLLKKSGKLPTWVKPFLRGITETW 77 L D IS+N D + +LLK++ LP W+ F +GI W Sbjct: 13 EALERDIISQNPNVRWDDIADLVEAKKLLKEAVVLPMWMPEFFKGIRRPW 62 >6B5C-A nrPDB Length = 307 Score = 27.7 bits (60), Expect = 2.6, Method: Compositional matrix adjust. Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 5/50 (10%) Query: 33 HVLSIDTISRNV----DQEGNLRTTR-LLKKSGKLPTWVKPFLRGITETW 77 L D +SRN D +L + LL+++ LP W+ F +GI W Sbjct: 9 EALERDIVSRNPSIHWDDIADLEEAKKLLREAVVLPMWMPDFFKGIRRPW 58 >4ZEN-A nrPDB Length = 485 Score = 27.3 bits (59), Expect = 3.4, Method: Compositional matrix adjust. Identities = 20/71 (28%), Positives = 29/71 (40%), Gaps = 11/71 (15%) Query: 102 GIMKVEEYTTYQFDSATSSTIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMA 161 G + V+ Y YQ D A + + +FS WSR D N + G+ Sbjct: 61 GDVAVDHYHRYQEDVALMAEMGLKAYRFSV-----------SWSRVFPDGNGAVNEKGLD 109 Query: 162 FVIQKLEEARN 172 F + +EE RN Sbjct: 110 FYDRLIEELRN 120 >5IMW-A nrPDB Length = 471 Score = 27.3 bits (59), Expect = 3.7, Method: Compositional matrix adjust. Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 4/57 (7%) Query: 81 VSVVNPANSTMKTYTRNLDHTGIMKVEEY----TTYQFDSATSSTIADSRVKFSSGF 133 ++V NP+NST++T NL I K + Q++S ++ +++ + KF + F Sbjct: 115 LTVENPSNSTVRTAVNNLVEKWIQKYSKTHAVPARMQYESISAQSMSQLQAKFGADF 171 >6AI8-A nrPDB Length = 371 Score = 26.6 bits (57), Expect = 6.8, Method: Compositional matrix adjust. Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 7/49 (14%) Query: 40 ISRNVDQEGNLRTTRLLKKSGKLPTWVKP---FLRGITETWIIEVSVVN 85 + N D EG RTT GKL + P FLR + E+W + +V+ Sbjct: 241 MGANNDTEGTTRTT----PDGKLIVNLDPVPKFLRRLVESWATQAMIVS 285 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5JQM-A nrPDB 269 4e-73 4XHR-A nrPDB 258 1e-69 6I3V-F nrPDB 242 9e-65 6I4Y-B nrPDB 237 2e-63 4XZV-B nrPDB 235 9e-63 Sequences not found previously or not previously below threshold: 1PQ3-A nrPDB 29 1.1 3ZVX-A nrPDB 28 1.9 6D81-A nrPDB 27 3.9 CONVERGED! >5JQM-A nrPDB Length = 283 Score = 269 bits (688), Expect = 4e-73, Method: Composition-based stats. Identities = 175/175 (100%), Positives = 175/175 (100%) Query: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG Sbjct: 15 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 74 Query: 61 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS Sbjct: 75 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 134 Query: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 175 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF Sbjct: 135 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 189 >4XHR-A nrPDB Length = 189 Score = 258 bits (658), Expect = 1e-69, Method: Composition-based stats. Identities = 175/175 (100%), Positives = 175/175 (100%) Query: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG Sbjct: 15 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 74 Query: 61 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS Sbjct: 75 KLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 134 Query: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 175 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF Sbjct: 135 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEARNPQF 189 >6I3V-F nrPDB Length = 182 Score = 242 bits (616), Expect = 9e-65, Method: Composition-based stats. Identities = 54/170 (31%), Positives = 88/170 (51%), Gaps = 2/170 (1%) Query: 1 MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSG 60 MV + + + V AF+ RYPNPYS HVL+ D + R V + L + RLL K+ Sbjct: 10 MVKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLLSRRLLTKTN 69 Query: 61 KLPTWVKPFLRG--ITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSAT 118 ++P W + +++E S+V+P N TM T+T N++H +M VEE + Y +S Sbjct: 70 RMPRWAERLFPANVAHSVYVLEDSIVDPQNQTMTTFTWNINHARLMVVEERSVYSVNSDN 129 Query: 119 SSTIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLE 168 S R + S G+ V+++ +F NV K+ G +++ KL+ Sbjct: 130 SGWTEIRREAWVSSSLFGVSRAVQEFGLARFKSNVTKTMKGFEYILAKLQ 179 >6I4Y-B nrPDB Length = 207 Score = 237 bits (605), Expect = 2e-63, Method: Composition-based stats. Identities = 47/169 (27%), Positives = 82/169 (48%), Gaps = 1/169 (0%) Query: 2 VLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSGK 61 + + S H+F + +V+ A +YPNP +P V+ +D + R++D G L + RLL Sbjct: 14 MKIWTSEHVFDHPWETVTTAAMQKYPNPMNPSVVGVDVLDRHIDPSGKLHSHRLLSTEWG 73 Query: 62 LPTWVKPFLRGI-TETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120 LP+ VK + T+T++ E SVV+P TM+ + N+ T ++ V+E Y+ Sbjct: 74 LPSIVKSLIGAARTKTYVQEHSVVDPVEKTMELKSTNISFTNMVSVDERLIYKPHPQDPE 133 Query: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEE 169 ++ + + + S +E + N K R M +VI KL Sbjct: 134 KTVLTQEAIITVKGVSLSSYLEGLMASTISSNASKGREAMEWVIHKLNA 182 >4XZV-B nrPDB Length = 186 Score = 235 bits (598), Expect = 9e-63, Method: Composition-based stats. Identities = 47/170 (27%), Positives = 77/170 (45%), Gaps = 1/170 (0%) Query: 2 VLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSGK 61 + + S H+F + +V +A +YPNP +P VL +D + R VD G L + RLL Sbjct: 15 MKIWSSEHVFGHPWDTVIQAAMRKYPNPMNPSVLGVDVLQRRVDGRGRLHSLRLLSTEWG 74 Query: 62 LPTWVKPFLR-GITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTYQFDSATSS 120 LP+ V+ L T T+I E SVV+P M+ + N+ T ++ V E Y Sbjct: 75 LPSLVRAILGTSRTLTYIREHSVVDPVEKKMELCSTNITLTNLVSVNERLVYTPHPENPE 134 Query: 121 TIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEA 170 ++ + + + S +E N KK + ++I+ E A Sbjct: 135 MTVLTQEAIITVKGISLGSYLESLMANTISSNAKKGWAAIEWIIEHSESA 184 >1PQ3-A nrPDB Length = 306 Score = 29.0 bits (63), Expect = 1.1, Method: Composition-based stats. Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 15/81 (18%) Query: 45 DQEGNLRT-------TRLLKKSGKLP--TWVKPFLRGITETWIIEVSVVNPANSTMKTYT 95 GNL L K +LP +W+KP + + +I V P + +K Y Sbjct: 131 TSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYD 190 Query: 96 ------RNLDHTGIMKVEEYT 110 R++D GI KV E T Sbjct: 191 IQYFSMRDIDRLGIQKVMERT 211 >3ZVX-A nrPDB Length = 261 Score = 28.2 bits (61), Expect = 1.9, Method: Composition-based stats. Identities = 9/54 (16%), Positives = 22/54 (40%), Gaps = 1/54 (1%) Query: 30 YSPHVLSIDTISRNVDQEGNLRTTRLLKKSGKLPTWVKPFLRGITETWIIEVSV 83 Y+P +ID ++ D + + ++ + LP WV+ + ++ Sbjct: 185 YNPSTRTIDVVATYPDGQRYQLSH-VVDLTTILPEWVRVGFSAASGEQFQTHNL 237 >6D81-A nrPDB Length = 193 Score = 27.1 bits (58), Expect = 3.9, Method: Composition-based stats. Identities = 23/111 (20%), Positives = 48/111 (43%), Gaps = 12/111 (10%) Query: 18 VSRAFFNRY----PNPYSPHVLSIDTISRNVDQEGNLRTTRLLKKSGKLPTWVKPFLRGI 73 V F RY PNP P +S + IS + + + +LLKK + P+ + + Sbjct: 72 VLADAFKRYLLDLPNPVIPVAVSSELISLAPEVQSSEEYIQLLKKLIRSPSIPHQYW--L 129 Query: 74 TETWIIEVSVVNPANSTMKTYTRNLDHTGIMK-VEEYTTYQFDSATSSTIA 123 T ++++ +T ++NL + ++ + ++F +A+S Sbjct: 130 TLQYLLKHFF-----KLSQTSSKNLLNARVLSELFSPLLFRFPAASSENTE 175 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.303 0.124 0.317 Lambda K H 0.267 0.0385 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,824,165 Number of Sequences: 36641 Number of extensions: 194293 Number of successful extensions: 540 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 521 Number of HSP's gapped (non-prelim): 22 length of query: 175 length of database: 10,336,785 effective HSP length: 90 effective length of query: 85 effective length of database: 7,039,095 effective search space: 598323075 effective search space used: 598323075 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (20.9 bits) S2: 55 (25.9 bits)