BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q04712 (507 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5O7X-C nrPDB 1051 0.0 6AZH-A nrPDB 30 3.6 1K1Y-A nrPDB 28 9.2 >5O7X-C nrPDB Length = 507 Score = 1051 bits (2718), Expect = 0.0, Method: Compositional matrix adjust. Identities = 507/507 (100%), Positives = 507/507 (100%) Query: 1 MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEG 60 MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEG Sbjct: 1 MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEG 60 Query: 61 QNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKED 120 QNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKED Sbjct: 61 QNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKED 120 Query: 121 SDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHC 180 SDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHC Sbjct: 121 SDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHC 180 Query: 181 TKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLI 240 TKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLI Sbjct: 181 TKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLI 240 Query: 241 RIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTG 300 RIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTG Sbjct: 241 RIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTG 300 Query: 301 SRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFME 360 SRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFME Sbjct: 301 SRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFME 360 Query: 361 DAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLD 420 DAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLD Sbjct: 361 DAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLD 420 Query: 421 KGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEF 480 KGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEF Sbjct: 421 KGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEF 480 Query: 481 LDTQLMDLSPEDNGLDEMHYSDEDSSE 507 LDTQLMDLSPEDNGLDEMHYSDEDSSE Sbjct: 481 LDTQLMDLSPEDNGLDEMHYSDEDSSE 507 >6AZH-A nrPDB Length = 192 Score = 29.6 bits (65), Expect = 3.6, Method: Compositional matrix adjust. Identities = 30/115 (26%), Positives = 49/115 (42%), Gaps = 25/115 (21%) Query: 136 KSFEIWRT----VSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHCTKENKLYFQSI 191 K EIW + + +PI K K L KM+ LL+ EN+ +F+ + Sbjct: 60 KGLEIWHEKLTDIENLEDEPIEKLK------------KLFKMQFELLY---ENRAFFKMV 104 Query: 192 SRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWS------LAYKIFATLI 240 L +T E+RN T +I + +L +S+ LA+ +F +LI Sbjct: 105 MSQLWGKETRQDELRNKITEYIEGIERILKEAISKKQIRECDISLLAHSLFGSLI 159 >1K1Y-A nrPDB Length = 659 Score = 28.1 bits (61), Expect = 9.2, Method: Compositional matrix adjust. Identities = 9/32 (28%), Positives = 20/32 (62%) Query: 313 LWSSLINCQKSMEPSSNIIDKPFDTENDLLQE 344 +W ++I Q+ ++P + I+D FD +++ E Sbjct: 373 VWENIIKAQRYLKPENKILDVDFDGRAEIMVE 404 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5O7X-C nrPDB 1077 0.0 Sequences not found previously or not previously below threshold: 6GMH-U nrPDB 37 0.018 6AZH-A nrPDB 33 0.28 3JCM-G nrPDB 32 0.71 5A5T-C nrPDB 32 0.79 6HIX-Ag nrPDB 31 1.2 6C95-D nrPDB 29 3.6 CONVERGED! >5O7X-C nrPDB Length = 507 Score = 1077 bits (2785), Expect = 0.0, Method: Composition-based stats. Identities = 507/507 (100%), Positives = 507/507 (100%) Query: 1 MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEG 60 MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEG Sbjct: 1 MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSLPTPENSAAENNDEEEG 60 Query: 61 QNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKED 120 QNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKED Sbjct: 61 QNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKED 120 Query: 121 SDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHC 180 SDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHC Sbjct: 121 SDEERKFWKKYEKPEKSFEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHC 180 Query: 181 TKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLI 240 TKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLI Sbjct: 181 TKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLI 240 Query: 241 RIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTG 300 RIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTG Sbjct: 241 RIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTG 300 Query: 301 SRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFME 360 SRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFME Sbjct: 301 SRTHTAKFAITYLWSSLINCQKSMEPSSNIIDKPFDTENDLLQELIDKISEWVLTPPFME 360 Query: 361 DAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLD 420 DAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLD Sbjct: 361 DAEVWFIYASCHLLKADTLSRQFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLD 420 Query: 421 KGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEF 480 KGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEF Sbjct: 421 KGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEF 480 Query: 481 LDTQLMDLSPEDNGLDEMHYSDEDSSE 507 LDTQLMDLSPEDNGLDEMHYSDEDSSE Sbjct: 481 LDTQLMDLSPEDNGLDEMHYSDEDSSE 507 >6GMH-U nrPDB Length = 776 Score = 37.0 bits (84), Expect = 0.018, Method: Composition-based stats. Identities = 30/120 (25%), Positives = 54/120 (45%), Gaps = 6/120 (5%) Query: 35 SKKSTTTDSLPTP----ENSAAENNDEEEGQNSEAGTYRRSVL-QQKKRRRERHWRSVVG 89 S++S D+ P+ S A N+DE+EG S+ G++ +K + W Sbjct: 201 SERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHSDDEKWGREDK 260 Query: 90 EIYSTTESETDSQEEETEEGGEHDTGIDKEDSDEERKFWKKYEKPEKSFEIWRTVSSQNK 149 S E +S +EE +G + D + D DE+ + E+P+ S + + +S + K Sbjct: 261 SDQSDDEKIQNSDDEERAQGSDEDK-LQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEK 319 >6AZH-A nrPDB Length = 192 Score = 33.1 bits (74), Expect = 0.28, Method: Composition-based stats. Identities = 29/115 (25%), Positives = 49/115 (42%), Gaps = 25/115 (21%) Query: 136 KSFEIWRT----VSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHCTKENKLYFQSI 191 K EIW + + +PI K K + KM+ LL+ EN+ +F+ + Sbjct: 60 KGLEIWHEKLTDIENLEDEPIEKLKKLF------------KMQFELLY---ENRAFFKMV 104 Query: 192 SRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRHNWS------LAYKIFATLI 240 L +T E+RN T +I + +L +S+ LA+ +F +LI Sbjct: 105 MSQLWGKETRQDELRNKITEYIEGIERILKEAISKKQIRECDISLLAHSLFGSLI 159 >3JCM-G nrPDB Length = 899 Score = 32.0 bits (71), Expect = 0.71, Method: Composition-based stats. Identities = 21/90 (23%), Positives = 40/90 (44%), Gaps = 25/90 (27%) Query: 420 DKGGFAVPSRLIENQLKSFESRLYGEAQDIQERDVANVY--------------------- 458 D G +P R EN + L + +D ++ + ANV+ Sbjct: 37 DDKGRRIPKRYREN----LNNHLQSQPKDDEDDEAANVFKTLELKLAQKKKKRANEKDDD 92 Query: 459 DSIDNSSVENSFGDVYETNAEFLDTQLMDL 488 +S+D+S+V+ F D+ E+ A +++ MD+ Sbjct: 93 NSVDSSNVKRQFADLKESLAAVTESEWMDI 122 >5A5T-C nrPDB Length = 843 Score = 31.6 bits (70), Expect = 0.79, Method: Composition-based stats. Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 3/54 (5%) Query: 71 SVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKEDSDEE 124 S L+QK R+ R + S + E D E+ EE E G ED DE+ Sbjct: 69 STLRQKIRKYNRDFESHITNYKQNPEQSADEDAEKNEEDSE---GSSDEDEDED 119 >6HIX-Ag nrPDB Length = 260 Score = 31.2 bits (69), Expect = 1.2, Method: Composition-based stats. Identities = 22/96 (22%), Positives = 45/96 (46%), Gaps = 11/96 (11%) Query: 55 NDEEEGQNSEAGTYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDT 114 N E E + +EA +RR + E+ W VG+ YS ++ +E+E + T Sbjct: 119 NYETERRRTEAPQWRRKL-------SEKEWDYYVGQ-YS---AQMKQEEDEIQRRVADRT 167 Query: 115 GIDKEDSDEERKFWKKYEKPEKSFEIWRTVSSQNKQ 150 + ++ + E ++ WK++ P ++ +S +Q Sbjct: 168 CVQEQSTREVQERWKRHVVPRMQTDLEFNLSHFKRQ 203 >6C95-D nrPDB Length = 129 Score = 29.3 bits (64), Expect = 3.6, Method: Composition-based stats. Identities = 26/106 (24%), Positives = 47/106 (44%), Gaps = 4/106 (3%) Query: 78 RRRERHWRSVVGEIYSTTESETDSQEEETEEGGEHDTGIDKEDSDEERKFWKKYEKPEKS 137 RRR + G++ E+ET E E+ +HD+G +D ER EK +S Sbjct: 2 RRRGEIDMATEGDVELELETETSGPERPPEKPRKHDSGA----ADLERVTDYAEEKEIQS 57 Query: 138 FEIWRTVSSQNKQPINKQKMTYHNFKKIEKIPLRKMEIPLLHCTKE 183 + +S + +QK K++ K+ ++K ++ L+ E Sbjct: 58 SNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEME 103 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.131 0.381 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,287,597 Number of Sequences: 36641 Number of extensions: 845866 Number of successful extensions: 2637 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 38 Number of HSP's that attempted gapping in prelim test: 2515 Number of HSP's gapped (non-prelim): 123 length of query: 507 length of database: 10,336,785 effective HSP length: 101 effective length of query: 406 effective length of database: 6,636,044 effective search space: 2694233864 effective search space used: 2694233864 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 61 (28.1 bits)