BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q03784 (219 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3CUE-A nrPDB 456 e-129 2J3T-D nrPDB 80 7e-16 1H3Q-A nrPDB 32 0.21 2J3T-C nrPDB 28 3.7 >3CUE-A nrPDB Length = 219 Score = 456 bits (1172), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/219 (100%), Positives = 219/219 (100%) Query: 1 MAIETILVINKSGGLIYQRNFTNDEQKLNSNEYLILASTLHGVFAIASQLTPKALQLTQQ 60 MAIETILVINKSGGLIYQRNFTNDEQKLNSNEYLILASTLHGVFAIASQLTPKALQLTQQ Sbjct: 1 MAIETILVINKSGGLIYQRNFTNDEQKLNSNEYLILASTLHGVFAIASQLTPKALQLTQQ 60 Query: 61 TNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKSGLR 120 TNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKSGLR Sbjct: 61 TNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKSGLR 120 Query: 121 QLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNFLR 180 QLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNFLR Sbjct: 121 QLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNFLR 180 Query: 181 KVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENLQ 219 KVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENLQ Sbjct: 181 KVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENLQ 219 >2J3T-D nrPDB Length = 219 Score = 80.1 bits (196), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 69/237 (29%), Positives = 105/237 (44%), Gaps = 51/237 (21%) Query: 1 MAIETILVINKSGGLIYQ-----------RNFT---NDEQKLNSNEYLILASTLHGVFAI 46 MAI ++ V+NK+GGLIYQ + F+ + KL+ L+ G+ Sbjct: 1 MAIFSVYVVNKAGGLIYQLDSYAPRAEAEKTFSYPLDLLLKLHDERVLVAFGQRDGIRVG 60 Query: 47 ASQLTPKALQLTQQTNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDF 106 + L + + + + + Y+G +N + G + T+NEKL Sbjct: 61 HAVLAINGMDVNGRYTADGK-EVLEYLGNPANYPVSIRFG--RPRLTSNEKL---MLASM 114 Query: 107 FKEPFT-------NWNKSGLRQLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTD 159 F F SG+ L TD F + YQTLTG+KFV ++ RQ I Sbjct: 115 FHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADP----RQAGI---- 166 Query: 160 KPDRPKSTSNLAIQIADNFLRKVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVE 216 D+ LRK+Y +YSD+ +K+P YS+EMPIR LFD+ +K +E Sbjct: 167 ----------------DSLLRKIYEIYSDFALKNPFYSLEMPIRCELFDQNLKLALE 207 >1H3Q-A nrPDB Length = 140 Score = 32.0 bits (71), Expect = 0.21, Method: Compositional matrix adjust. Identities = 17/36 (47%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Query: 177 NFLRKVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVK 212 NF VY LY + M +P Y PIRS+ FD KV+ Sbjct: 97 NFFTDVYDLYIKFAM-NPFYEPNSPIRSSAFDRKVQ 131 >2J3T-C nrPDB Length = 145 Score = 27.7 bits (60), Expect = 3.7, Method: Compositional matrix adjust. Identities = 22/104 (21%), Positives = 42/104 (40%), Gaps = 25/104 (24%) Query: 116 KSGLRQLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIA 175 K G T ++ + Y+T TG+K V ++L + Sbjct: 58 KDGFLSFQTSRYKLHYYETPTGIKVVM------------------------NTDLGVGPI 93 Query: 176 DNFLRKVY-CLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENL 218 + L +Y LY ++V+K+P + ++S LF ++ V +L Sbjct: 94 RDVLHHIYSALYVEFVVKNPLCPLGQTVQSELFRSRLDSYVRSL 137 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3CUE-A nrPDB 354 2e-98 2J3T-D nrPDB 266 6e-72 Sequences not found previously or not previously below threshold: 2J3T-C nrPDB 38 0.004 1H3Q-A nrPDB 37 0.005 1YHU-D nrPDB 31 0.39 1JQG-A nrPDB 30 0.56 3WCU-C nrPDB 30 0.85 1ZB7-A nrPDB 29 1.8 5DF0-A nrPDB 27 4.4 CONVERGED! >3CUE-A nrPDB Length = 219 Score = 354 bits (907), Expect = 2e-98, Method: Composition-based stats. Identities = 219/219 (100%), Positives = 219/219 (100%) Query: 1 MAIETILVINKSGGLIYQRNFTNDEQKLNSNEYLILASTLHGVFAIASQLTPKALQLTQQ 60 MAIETILVINKSGGLIYQRNFTNDEQKLNSNEYLILASTLHGVFAIASQLTPKALQLTQQ Sbjct: 1 MAIETILVINKSGGLIYQRNFTNDEQKLNSNEYLILASTLHGVFAIASQLTPKALQLTQQ 60 Query: 61 TNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKSGLR 120 TNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKSGLR Sbjct: 61 TNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKSGLR 120 Query: 121 QLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNFLR 180 QLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNFLR Sbjct: 121 QLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNFLR 180 Query: 181 KVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENLQ 219 KVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENLQ Sbjct: 181 KVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENLQ 219 >2J3T-D nrPDB Length = 219 Score = 266 bits (679), Expect = 6e-72, Method: Composition-based stats. Identities = 69/237 (29%), Positives = 105/237 (44%), Gaps = 51/237 (21%) Query: 1 MAIETILVINKSGGLIYQ-----------RNFT---NDEQKLNSNEYLILASTLHGVFAI 46 MAI ++ V+NK+GGLIYQ + F+ + KL+ L+ G+ Sbjct: 1 MAIFSVYVVNKAGGLIYQLDSYAPRAEAEKTFSYPLDLLLKLHDERVLVAFGQRDGIRVG 60 Query: 47 ASQLTPKALQLTQQTNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDF 106 + L + + + + + Y+G +N + G + T+NEKL Sbjct: 61 HAVLAINGMDVNGRYTADGK-EVLEYLGNPANYPVSIRFG--RPRLTSNEKL---MLASM 114 Query: 107 FKEPFT-------NWNKSGLRQLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTD 159 F F SG+ L TD F + YQTLTG+KFV ++ RQ I Sbjct: 115 FHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADP----RQAGI---- 166 Query: 160 KPDRPKSTSNLAIQIADNFLRKVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVE 216 D+ LRK+Y +YSD+ +K+P YS+EMPIR LFD+ +K +E Sbjct: 167 ----------------DSLLRKIYEIYSDFALKNPFYSLEMPIRCELFDQNLKLALE 207 >2J3T-C nrPDB Length = 145 Score = 37.8 bits (86), Expect = 0.004, Method: Composition-based stats. Identities = 22/104 (21%), Positives = 41/104 (39%), Gaps = 25/104 (24%) Query: 116 KSGLRQLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIA 175 K G T ++ + Y+T TG+K V + L + Sbjct: 58 KDGFLSFQTSRYKLHYYETPTGIKVVMNTD------------------------LGVGPI 93 Query: 176 DNFLRKVYC-LYSDYVMKDPSYSMEMPIRSNLFDEKVKKMVENL 218 + L +Y LY ++V+K+P + ++S LF ++ V +L Sbjct: 94 RDVLHHIYSALYVEFVVKNPLCPLGQTVQSELFRSRLDSYVRSL 137 >1H3Q-A nrPDB Length = 140 Score = 37.4 bits (85), Expect = 0.005, Method: Composition-based stats. Identities = 17/36 (47%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Query: 177 NFLRKVYCLYSDYVMKDPSYSMEMPIRSNLFDEKVK 212 NF VY LY + M +P Y PIRS+ FD KV+ Sbjct: 97 NFFTDVYDLYIKFAM-NPFYEPNSPIRSSAFDRKVQ 131 >1YHU-D nrPDB Length = 149 Score = 30.9 bits (68), Expect = 0.39, Method: Composition-based stats. Identities = 18/92 (19%), Positives = 33/92 (35%), Gaps = 9/92 (9%) Query: 107 FKEPFTN-------WNKSGLRQLCTDQFTMFIYQTLTGLKFVA--ISSSVMPQRQPTIAT 157 FK+ F N + ++ +++F + + GL +S Q + Sbjct: 36 FKDLFANVPDAVGLFGAVKGDEVNSNEFKAHCIRVVNGLDSSIGLLSDPATLNEQLSHLA 95 Query: 158 TDKPDRPKSTSNLAIQIADNFLRKVYCLYSDY 189 T R T IA +FLR + + S + Sbjct: 96 TQHKARSGVTKGGFSAIAQSFLRVMPQVASCF 127 >1JQG-A nrPDB Length = 433 Score = 30.5 bits (67), Expect = 0.56, Method: Composition-based stats. Identities = 24/104 (23%), Positives = 39/104 (37%), Gaps = 13/104 (12%) Query: 28 LNSNEYLILASTLHGVFAIASQLTPKALQLTQQTNIENTIPYIPYVGMSSNRSDTRNGGG 87 L+ E++ L +TL +AI + + +T+ I N I V T GG Sbjct: 180 LHCREWVTLPATL---YAIHKLV----IDVTESDLINNIDWIILPVANPDGYVHT--FGG 230 Query: 88 NN----NKHTNNEKLGSFKGDDFFKEPFTNWNKSGLRQLCTDQF 127 + N+ T G D + NW + +C+D F Sbjct: 231 DRYWRKNRATGYMAGNLCMGVDLNRNFGMNWGTASSSSVCSDTF 274 >3WCU-C nrPDB Length = 150 Score = 30.1 bits (66), Expect = 0.85, Method: Composition-based stats. Identities = 14/71 (19%), Positives = 27/71 (38%), Gaps = 2/71 (2%) Query: 121 QLCTDQFTMFIYQTLTGLKFVA--ISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIADNF 178 ++ + +F + + GL +S Q + T +R T IA +F Sbjct: 58 EIDSSEFKAHCIRVVNGLDSAIGLLSDPSTLNEQLSHLATQHQERAGVTKGGFSAIAQSF 117 Query: 179 LRKVYCLYSDY 189 LR + + S + Sbjct: 118 LRVMPQVASCF 128 >1ZB7-A nrPDB Length = 455 Score = 29.0 bits (63), Expect = 1.8, Method: Composition-based stats. Identities = 18/71 (25%), Positives = 30/71 (42%), Gaps = 2/71 (2%) Query: 58 TQQTNIENTIPYIPYVGMSSNRSDTRNGGGNNNKHTNNEKLGSFKGDDFFKEPFTNWNKS 117 + Q ++ + IPY+G +S D N + N+K+ +D K TN Sbjct: 104 SGQRLLDMIVDAIPYLGNASTPPD--KFAANVANVSINKKIIQPGAEDQIKGLMTNLIIF 161 Query: 118 GLRQLCTDQFT 128 G + +D FT Sbjct: 162 GPGPVLSDNFT 172 >5DF0-A nrPDB Length = 545 Score = 27.4 bits (59), Expect = 4.4, Method: Composition-based stats. Identities = 17/78 (21%), Positives = 38/78 (48%), Gaps = 3/78 (3%) Query: 141 VAISSSVMPQRQP--TIATTDKPDRPKSTSNLAIQIADNFLRKVYCLYSDYVMKDPSYSM 198 VAI ++P +++ ++P + +A ++A+ L K+ + + DP Y M Sbjct: 216 VAIHDPWAAVQKPQKSVSAWNEPYKGNFGQLMAAKLANPHL-KILPSIGGWTLSDPFYFM 274 Query: 199 EMPIRSNLFDEKVKKMVE 216 + N+F + VK+ ++ Sbjct: 275 HDVEKRNVFVDSVKEFLQ 292 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.127 0.329 Lambda K H 0.267 0.0386 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,224,006 Number of Sequences: 36641 Number of extensions: 251059 Number of successful extensions: 737 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 707 Number of HSP's gapped (non-prelim): 26 length of query: 219 length of database: 10,336,785 effective HSP length: 93 effective length of query: 126 effective length of database: 6,929,172 effective search space: 873075672 effective search space used: 873075672 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.7 bits)