BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q03782 (492 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5ZWN-W nrPDB 1027 0.0 6B4R-B nrPDB 32 0.49 3DTP-E nrPDB 32 0.76 3I81-A nrPDB 28 6.8 >5ZWN-W nrPDB Length = 492 Score = 1027 bits (2655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 492/492 (100%), Positives = 492/492 (100%) Query: 1 MRPRRRGLAYHHTKPKGQLSQGHYPTTSNDGQRRKVGNSEAFQSFDIWKNLDRIRSTKKN 60 MRPRRRGLAYHHTKPKGQLSQGHYPTTSNDGQRRKVGNSEAFQSFDIWKNLDRIRSTKKN Sbjct: 1 MRPRRRGLAYHHTKPKGQLSQGHYPTTSNDGQRRKVGNSEAFQSFDIWKNLDRIRSTKKN 60 Query: 61 AGQFIKGSLLILPMRTEDKQQFDECMDELHKYISKDILRCYPQKEQKDEGMLFYIVLKDF 120 AGQFIKGSLLILPMRTEDKQQFDECMDELHKYISKDILRCYPQKEQKDEGMLFYIVLKDF Sbjct: 61 AGQFIKGSLLILPMRTEDKQQFDECMDELHKYISKDILRCYPQKEQKDEGMLFYIVLKDF 120 Query: 121 NILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRR 180 NILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRR Sbjct: 121 NILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRR 180 Query: 181 EQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFSQVEFGKCEANVINYFKREYN 240 EQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFSQVEFGKCEANVINYFKREYN Sbjct: 181 EQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFSQVEFGKCEANVINYFKREYN 240 Query: 241 RTYSQISLALYELPLIGDGLFDIKSYISKTRPIIETSKAQMIKHISEMKAYNEISGLQGD 300 RTYSQISLALYELPLIGDGLFDIKSYISKTRPIIETSKAQMIKHISEMKAYNEISGLQGD Sbjct: 241 RTYSQISLALYELPLIGDGLFDIKSYISKTRPIIETSKAQMIKHISEMKAYNEISGLQGD 300 Query: 301 QFPRQQRPLSNSPSSNSISSSQTIEAGATSYQTQPQRHAVNKPSNVLNSSNRHSGPKTFE 360 QFPRQQRPLSNSPSSNSISSSQTIEAGATSYQTQPQRHAVNKPSNVLNSSNRHSGPKTFE Sbjct: 301 QFPRQQRPLSNSPSSNSISSSQTIEAGATSYQTQPQRHAVNKPSNVLNSSNRHSGPKTFE 360 Query: 361 DGRYSEGNKPGFMTQDEIKQHCIGTIKASMDAVKKKSSYQILKTYVRCPRQNYIDIVYQN 420 DGRYSEGNKPGFMTQDEIKQHCIGTIKASMDAVKKKSSYQILKTYVRCPRQNYIDIVYQN Sbjct: 361 DGRYSEGNKPGFMTQDEIKQHCIGTIKASMDAVKKKSSYQILKTYVRCPRQNYIDIVYQN 420 Query: 421 LNDLRSKTNCNIVVLNLNNLHESQMWLESLNTTNYTIFAQAPHPSTIRVISIGGVGEYIV 480 LNDLRSKTNCNIVVLNLNNLHESQMWLESLNTTNYTIFAQAPHPSTIRVISIGGVGEYIV Sbjct: 421 LNDLRSKTNCNIVVLNLNNLHESQMWLESLNTTNYTIFAQAPHPSTIRVISIGGVGEYIV 480 Query: 481 KALELILNILEH 492 KALELILNILEH Sbjct: 481 KALELILNILEH 492 >6B4R-B nrPDB Length = 497 Score = 32.3 bits (72), Expect = 0.49, Method: Compositional matrix adjust. Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 3/69 (4%) Query: 110 GMLFYIVLKDFNILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPK---N 166 G++ IV +F +L SC+ L LA + + PSEKS + L +PK N Sbjct: 159 GVVAAIVPWNFPLLMSCWKLGPALATGNSVILKPSEKSPLTAIRIAQLAVEAGIPKGVFN 218 Query: 167 IETGRGHII 175 + G GH + Sbjct: 219 VLPGYGHTV 227 >3DTP-E nrPDB Length = 196 Score = 31.6 bits (70), Expect = 0.76, Method: Compositional matrix adjust. Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 3/52 (5%) Query: 217 NFFSQVEFGKCEANVINYFKREYNRTYSQ--ISLALYELPLIGDGLFDIKSY 266 N F + + GKC+ + + +SQ + AL E P+ G+GL DIK + Sbjct: 133 NLFDEGD-GKCKEETLKRSLTTWGEKFSQDEVDQALSEAPIDGNGLIDIKKF 183 >3I81-A nrPDB Length = 315 Score = 28.5 bits (62), Expect = 6.8, Method: Compositional matrix adjust. Identities = 37/139 (26%), Positives = 58/139 (41%), Gaps = 17/139 (12%) Query: 158 TGSFYLPKNIETGRGHIITSYRREQPSSSIVEVGF--NVVPDFQQFQVKACHVSKFMNEL 215 Y+P E R I S Q S +V G VV D + +V K +NE Sbjct: 4 AADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAI----KTVNEA 59 Query: 216 SNFFSQVEFGKCEANVINYFKREYNR------TYSQISLALYELPLIGDGLFDIKSYISK 269 ++ ++EF EA+V+ F + + Q +L + EL GD +KSY+ Sbjct: 60 ASMRERIEFLN-EASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGD----LKSYLRS 114 Query: 270 TRPIIETSKAQMIKHISEM 288 RP +E + +S+M Sbjct: 115 LRPEMENNPVLAPPSLSKM 133 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5ZWN-W nrPDB 1057 0.0 Sequences not found previously or not previously below threshold: 3DTP-E nrPDB 30 1.6 1KBP-A nrPDB 30 2.1 3I81-A nrPDB 30 2.2 6B4R-B nrPDB 30 2.6 4X51-A nrPDB 28 7.4 CONVERGED! >5ZWN-W nrPDB Length = 492 Score = 1057 bits (2734), Expect = 0.0, Method: Composition-based stats. Identities = 492/492 (100%), Positives = 492/492 (100%) Query: 1 MRPRRRGLAYHHTKPKGQLSQGHYPTTSNDGQRRKVGNSEAFQSFDIWKNLDRIRSTKKN 60 MRPRRRGLAYHHTKPKGQLSQGHYPTTSNDGQRRKVGNSEAFQSFDIWKNLDRIRSTKKN Sbjct: 1 MRPRRRGLAYHHTKPKGQLSQGHYPTTSNDGQRRKVGNSEAFQSFDIWKNLDRIRSTKKN 60 Query: 61 AGQFIKGSLLILPMRTEDKQQFDECMDELHKYISKDILRCYPQKEQKDEGMLFYIVLKDF 120 AGQFIKGSLLILPMRTEDKQQFDECMDELHKYISKDILRCYPQKEQKDEGMLFYIVLKDF Sbjct: 61 AGQFIKGSLLILPMRTEDKQQFDECMDELHKYISKDILRCYPQKEQKDEGMLFYIVLKDF 120 Query: 121 NILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRR 180 NILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRR Sbjct: 121 NILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRR 180 Query: 181 EQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFSQVEFGKCEANVINYFKREYN 240 EQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFSQVEFGKCEANVINYFKREYN Sbjct: 181 EQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFSQVEFGKCEANVINYFKREYN 240 Query: 241 RTYSQISLALYELPLIGDGLFDIKSYISKTRPIIETSKAQMIKHISEMKAYNEISGLQGD 300 RTYSQISLALYELPLIGDGLFDIKSYISKTRPIIETSKAQMIKHISEMKAYNEISGLQGD Sbjct: 241 RTYSQISLALYELPLIGDGLFDIKSYISKTRPIIETSKAQMIKHISEMKAYNEISGLQGD 300 Query: 301 QFPRQQRPLSNSPSSNSISSSQTIEAGATSYQTQPQRHAVNKPSNVLNSSNRHSGPKTFE 360 QFPRQQRPLSNSPSSNSISSSQTIEAGATSYQTQPQRHAVNKPSNVLNSSNRHSGPKTFE Sbjct: 301 QFPRQQRPLSNSPSSNSISSSQTIEAGATSYQTQPQRHAVNKPSNVLNSSNRHSGPKTFE 360 Query: 361 DGRYSEGNKPGFMTQDEIKQHCIGTIKASMDAVKKKSSYQILKTYVRCPRQNYIDIVYQN 420 DGRYSEGNKPGFMTQDEIKQHCIGTIKASMDAVKKKSSYQILKTYVRCPRQNYIDIVYQN Sbjct: 361 DGRYSEGNKPGFMTQDEIKQHCIGTIKASMDAVKKKSSYQILKTYVRCPRQNYIDIVYQN 420 Query: 421 LNDLRSKTNCNIVVLNLNNLHESQMWLESLNTTNYTIFAQAPHPSTIRVISIGGVGEYIV 480 LNDLRSKTNCNIVVLNLNNLHESQMWLESLNTTNYTIFAQAPHPSTIRVISIGGVGEYIV Sbjct: 421 LNDLRSKTNCNIVVLNLNNLHESQMWLESLNTTNYTIFAQAPHPSTIRVISIGGVGEYIV 480 Query: 481 KALELILNILEH 492 KALELILNILEH Sbjct: 481 KALELILNILEH 492 >3DTP-E nrPDB Length = 196 Score = 30.4 bits (67), Expect = 1.6, Method: Composition-based stats. Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 3/52 (5%) Query: 217 NFFSQVEFGKCEANVINYFKREYNRTYSQ--ISLALYELPLIGDGLFDIKSY 266 N F + + GKC+ + + +SQ + AL E P+ G+GL DIK + Sbjct: 133 NLFDEGD-GKCKEETLKRSLTTWGEKFSQDEVDQALSEAPIDGNGLIDIKKF 183 >1KBP-A nrPDB Length = 432 Score = 30.0 bits (66), Expect = 2.1, Method: Composition-based stats. Identities = 31/125 (24%), Positives = 48/125 (38%), Gaps = 10/125 (8%) Query: 143 PSEKSYFRVPKNINLTGSFYLPKNIETGRGHIITSYRREQPSSSIVEVGFNVVPDFQQFQ 202 P + FRVP N ++ + GR II+ ++P SS V ++ ++ Sbjct: 12 PLDSDVFRVPPGYNAPQQVHITQGDLVGRAMIISWVTMDEPGSSAVRY-WSEKNGRKRIA 70 Query: 203 VKACHVSKFMNELSNFFSQVEFGKCEANVINYFK---REYNRTYS-----QISLAL-YEL 253 +F N S F K + N Y++ R R +S Q L + Y Sbjct: 71 KGKMSTYRFFNYSSGFIHHTTIRKLKYNTKYYYEVGLRNTTRRFSFITPPQTGLDVPYTF 130 Query: 254 PLIGD 258 LIGD Sbjct: 131 GLIGD 135 >3I81-A nrPDB Length = 315 Score = 30.0 bits (66), Expect = 2.2, Method: Composition-based stats. Identities = 37/135 (27%), Positives = 58/135 (42%), Gaps = 17/135 (12%) Query: 162 YLPKNIETGRGHIITSYRREQPSSSIVEVGF--NVVPDFQQFQVKACHVSKFMNELSNFF 219 Y+P E R I S Q S +V G VV D + +V K +NE ++ Sbjct: 8 YVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAI----KTVNEAASMR 63 Query: 220 SQVEFGKCEANVINYFKREYNR------TYSQISLALYELPLIGDGLFDIKSYISKTRPI 273 ++EF EA+V+ F + + Q +L + EL GD +KSY+ RP Sbjct: 64 ERIEFLN-EASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGD----LKSYLRSLRPE 118 Query: 274 IETSKAQMIKHISEM 288 +E + +S+M Sbjct: 119 MENNPVLAPPSLSKM 133 >6B4R-B nrPDB Length = 497 Score = 30.0 bits (66), Expect = 2.6, Method: Composition-based stats. Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 3/69 (4%) Query: 110 GMLFYIVLKDFNILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGSFYLPK---N 166 G++ IV +F +L SC+ L LA + + PSEKS + L +PK N Sbjct: 159 GVVAAIVPWNFPLLMSCWKLGPALATGNSVILKPSEKSPLTAIRIAQLAVEAGIPKGVFN 218 Query: 167 IETGRGHII 175 + G GH + Sbjct: 219 VLPGYGHTV 227 >4X51-A nrPDB Length = 162 Score = 28.5 bits (62), Expect = 7.4, Method: Composition-based stats. Identities = 30/123 (24%), Positives = 50/123 (40%), Gaps = 8/123 (6%) Query: 101 YPQKEQKDEGMLFYIVLKDFNILDSCFVLSVLLAFQKRLWMAPSEKSYFRVPKNINLTGS 160 + K+Q D +L +++DF C LS ++ F M +EK + +++N G Sbjct: 29 FQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGE 88 Query: 161 FYLPKNIETGRGHIITSYRREQPSSSIVEVGFNVVPDFQQFQVKACHVSKFMNELSNFFS 220 + R H R P + + V DF + Q + V K MNE F + Sbjct: 89 KLKTLRMRLRRCH------RFLPCENKSKAVEQVKSDFNKLQDQG--VYKAMNEFDIFIN 140 Query: 221 QVE 223 +E Sbjct: 141 YIE 143 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.133 0.389 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 19,639,272 Number of Sequences: 36641 Number of extensions: 807812 Number of successful extensions: 1950 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 1946 Number of HSP's gapped (non-prelim): 14 length of query: 492 length of database: 10,336,785 effective HSP length: 101 effective length of query: 391 effective length of database: 6,636,044 effective search space: 2594693204 effective search space used: 2594693204 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 61 (28.1 bits)