BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q03630 (159 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3CUE-C nrPDB 332 4e-92 2J3T-C nrPDB 80 3e-16 2J3T-D nrPDB 42 2e-04 5HB2-C nrPDB 28 1.9 3WDH-A nrPDB 27 3.2 1UHV-A nrPDB 27 4.1 4UEE-A nrPDB 26 6.3 5OM9-A nrPDB 26 6.7 2QTK-A nrPDB 26 8.2 >3CUE-C nrPDB Length = 159 Score = 332 bits (852), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 159/159 (100%), Positives = 159/159 (100%) Query: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK Sbjct: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 Query: 61 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL 120 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL Sbjct: 61 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL 120 Query: 121 SPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ 159 SPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ Sbjct: 121 SPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ 159 >2J3T-C nrPDB Length = 145 Score = 80.5 bits (197), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 41/122 (33%), Positives = 67/122 (54%), Gaps = 6/122 (4%) Query: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 M +++ ++FDR+ C+ EW + EE+ KL+YGM+FS+RS K+S Sbjct: 1 MTVHNLYLFDRNGVCLHYSEWHRKKQAGI------PKEEEYKLMYGMLFSIRSFVSKMSP 54 Query: 61 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL 120 +K+ S T +Y++H Y T +G+ V+ +D VL +IYS +YV++V N L Sbjct: 55 LDMKDGFLSFQTSRYKLHYYETPTGIKVVMNTDLGVGPIRDVLHHIYSALYVEFVVKNPL 114 Query: 121 SP 122 P Sbjct: 115 CP 116 >2J3T-D nrPDB Length = 219 Score = 41.6 bits (96), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 1/76 (1%) Query: 43 LLYGMIFSLRSITQKLSKGSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQV 102 +L M SL +I +LS + I + T +++H Y T +G+ FV+L+D +Q + Sbjct: 110 MLASMFHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADPRQAGIDSL 169 Query: 103 LQYIYSHIYVKYVSNN 118 L+ IY IY + N Sbjct: 170 LRKIY-EIYSDFALKN 184 >5HB2-C nrPDB Length = 723 Score = 28.1 bits (61), Expect = 1.9, Method: Composition-based stats. Identities = 18/58 (31%), Positives = 24/58 (41%), Gaps = 7/58 (12%) Query: 99 YTQVLQYIYSHIYVKYVS-------NNLLSPYDFAENENEMRGQGTRKITNRNFISVL 149 + Q + Y+Y YV V LL P D A NE+ TR + NF +L Sbjct: 333 FEQAIAYLYPFSYVDAVHFAIALTYYGLLRPVDAASAGNELLSHNTRSMPQINFGRML 390 >3WDH-A nrPDB Length = 710 Score = 27.3 bits (59), Expect = 3.2, Method: Composition-based stats. Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 2/46 (4%) Query: 94 FKQQSYTQVLQYIYSHIYVKYVSN--NLLSPYDFAENENEMRGQGT 137 F+Q+ +L +Y+H+YV+ S+ +L+ Y F N GT Sbjct: 332 FQQEGIRVILDVVYNHVYVRETSSFEHLVPGYYFRYERNGYPSNGT 377 >1UHV-A nrPDB Length = 500 Score = 26.9 bits (58), Expect = 4.1, Method: Composition-based stats. Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 5/75 (6%) Query: 80 YCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLLSPYDFAENENEMRGQGTRK 139 YC +G + L Q+ Y + L+Y+ +I KY+ + L D ++ G + Sbjct: 20 YCVGTGRLGLAL----QKEYIETLKYVKENIDFKYIRGHGLLCDDVGIYREDVVGDEVKP 75 Query: 140 ITNRNFIS-VLESFL 153 N +I + +SFL Sbjct: 76 FYNFTYIDRIFDSFL 90 >4UEE-A nrPDB Length = 309 Score = 26.2 bits (56), Expect = 6.3, Method: Compositional matrix adjust. Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 8/96 (8%) Query: 41 AKLLYGMIFSLRSI-TQKLSKGSVKNDIRSISTGKYRVHTYCTASGLWFV--LLSDFKQQ 97 +K+ G + R I K S G K I TG + ASG+WF + D+ Q Sbjct: 34 SKIQIGNTYEGRPIYVLKFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQD 93 Query: 98 -SYTQVLQYIYSHIYVKYVSNNLLSPYDFAENENEM 132 ++T +L + I+++ V+N + F + N M Sbjct: 94 AAFTAILDTL--DIFLEIVTNP--DGFAFTHSTNRM 125 >5OM9-A nrPDB Length = 419 Score = 26.2 bits (56), Expect = 6.7, Method: Compositional matrix adjust. Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 8/96 (8%) Query: 41 AKLLYGMIFSLRSI-TQKLSKGSVKNDIRSISTGKYRVHTYCTASGLWFV--LLSDFKQQ 97 +K+ G + R I K S G K I TG + ASG+WF + D+ Q Sbjct: 144 SKIQIGNTYEGRPIYVLKFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQD 203 Query: 98 -SYTQVLQYIYSHIYVKYVSNNLLSPYDFAENENEM 132 ++T +L + I+++ V+N + F + N M Sbjct: 204 AAFTAILDTL--DIFLEIVTNP--DGFAFTHSTNRM 235 >2QTK-A nrPDB Length = 390 Score = 25.8 bits (55), Expect = 8.2, Method: Compositional matrix adjust. Identities = 14/41 (34%), Positives = 21/41 (51%) Query: 64 KNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQ 104 K+D + + +YR + T GLW L D +QSY +L Sbjct: 175 KSDGFNYAGAEYRFNRERTQLGLWHGQLEDVYRQSYANLLH 215 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3CUE-C nrPDB 266 4e-72 2J3T-C nrPDB 187 2e-48 2J3T-D nrPDB 116 4e-27 Sequences not found previously or not previously below threshold: 3CUE-A nrPDB 37 0.005 2QTK-A nrPDB 27 5.1 3W43-A nrPDB 26 8.3 1T47-A nrPDB 26 8.5 CONVERGED! >3CUE-C nrPDB Length = 159 Score = 266 bits (679), Expect = 4e-72, Method: Composition-based stats. Identities = 159/159 (100%), Positives = 159/159 (100%) Query: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK Sbjct: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 Query: 61 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL 120 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL Sbjct: 61 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL 120 Query: 121 SPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ 159 SPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ Sbjct: 121 SPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ 159 >2J3T-C nrPDB Length = 145 Score = 187 bits (475), Expect = 2e-48, Method: Composition-based stats. Identities = 41/122 (33%), Positives = 67/122 (54%), Gaps = 6/122 (4%) Query: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 M +++ ++FDR+ C+ EW + EE+ KL+YGM+FS+RS K+S Sbjct: 1 MTVHNLYLFDRNGVCLHYSEWHRKKQAGI------PKEEEYKLMYGMLFSIRSFVSKMSP 54 Query: 61 GSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLL 120 +K+ S T +Y++H Y T +G+ V+ +D VL +IYS +YV++V N L Sbjct: 55 LDMKDGFLSFQTSRYKLHYYETPTGIKVVMNTDLGVGPIRDVLHHIYSALYVEFVVKNPL 114 Query: 121 SP 122 P Sbjct: 115 CP 116 >2J3T-D nrPDB Length = 219 Score = 116 bits (290), Expect = 4e-27, Method: Composition-based stats. Identities = 24/78 (30%), Positives = 40/78 (51%), Gaps = 1/78 (1%) Query: 43 LLYGMIFSLRSITQKLSKGSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQV 102 +L M SL +I +LS + I + T +++H Y T +G+ FV+L+D +Q + Sbjct: 110 MLASMFHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADPRQAGIDSL 169 Query: 103 LQYIYSHIYVKYVSNNLL 120 L+ IY IY + N Sbjct: 170 LRKIY-EIYSDFALKNPF 186 >3CUE-A nrPDB Length = 219 Score = 36.6 bits (83), Expect = 0.005, Method: Composition-based stats. Identities = 16/80 (20%), Positives = 29/80 (36%), Gaps = 25/80 (31%) Query: 64 KNDIRSISTGKYRVHTYCTASGLWFVLL------------------------SDFKQQSY 99 K+ +R + T ++ + Y T +GL FV + S+ Q Sbjct: 116 KSGLRQLCTDQFTMFIYQTLTGLKFVAISSSVMPQRQPTIATTDKPDRPKSTSNLAIQIA 175 Query: 100 TQVLQYIYSHIYVKYVSNNL 119 L+ +Y +Y YV + Sbjct: 176 DNFLRKVYC-LYSDYVMKDP 194 Score = 29.7 bits (65), Expect = 0.58, Method: Composition-based stats. Identities = 10/64 (15%), Positives = 26/64 (40%), Gaps = 8/64 (12%) Query: 1 MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLLYGMIFSLRSITQKLSK 60 M I + + ++ I+ R +T K N + +L + + +I +L+ Sbjct: 1 MAIETILVINKSGGLIYQRNFTNDEQ--------KLNSNEYLILASTLHGVFAIASQLTP 52 Query: 61 GSVK 64 +++ Sbjct: 53 KALQ 56 >2QTK-A nrPDB Length = 390 Score = 26.6 bits (57), Expect = 5.1, Method: Composition-based stats. Identities = 12/47 (25%), Positives = 20/47 (42%), Gaps = 1/47 (2%) Query: 59 SKGSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQVLQY 105 + + + + +YR + T GLW L D +QSY +L Sbjct: 171 APTQKSDGF-NYAGAEYRFNRERTQLGLWHGQLEDVYRQSYANLLHK 216 >3W43-A nrPDB Length = 199 Score = 25.8 bits (55), Expect = 8.3, Method: Composition-based stats. Identities = 15/85 (17%), Positives = 30/85 (35%), Gaps = 3/85 (3%) Query: 23 LASNSASGTINSKQNEEDAKLLYGMIFSLRS-ITQKLSKGSVKNDIRSISTGKYRVHTYC 81 + ++ IN +Q + + + F L S + + + S KY+ HT Sbjct: 86 RGATASILKINFEQRQFTYCSVGNVRFILHSPSGESFYPLPI-SGYLSGKPQKYKTHTAT 144 Query: 82 TASGLWFVLLSD-FKQQSYTQVLQY 105 G F++ +D L+ Sbjct: 145 YEKGSKFIIHTDGLNVPDIRSHLKK 169 >1T47-A nrPDB Length = 381 Score = 25.8 bits (55), Expect = 8.5, Method: Composition-based stats. Identities = 11/45 (24%), Positives = 22/45 (48%), Gaps = 1/45 (2%) Query: 78 HTYCTASGLWFVLLSDFKQQSYTQVLQYIYSHIYVKYVSNNLLSP 122 H Y TA G+ V S + S + Y+ ++ ++V +++ P Sbjct: 38 HYYSTAFGMQLVAYSGPENGS-RETASYVLTNGSARFVLTSVIKP 81 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.132 0.357 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,560,956 Number of Sequences: 36641 Number of extensions: 189812 Number of successful extensions: 432 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 415 Number of HSP's gapped (non-prelim): 25 length of query: 159 length of database: 10,336,785 effective HSP length: 89 effective length of query: 70 effective length of database: 7,075,736 effective search space: 495301520 effective search space used: 495301520 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)