BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q03433 (280 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6GEJ-S nrPDB 578 e-165 4AXH-A nrPDB 29 1.9 6I9E-A nrPDB 29 2.3 3QJ4-A nrPDB 27 8.8 >6GEJ-S nrPDB Length = 280 Score = 578 bits (1489), Expect = e-165, Method: Compositional matrix adjust. Identities = 280/280 (100%), Positives = 280/280 (100%) Query: 1 MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARL 60 MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARL Sbjct: 1 MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARL 60 Query: 61 YTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLL 120 YTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLL Sbjct: 61 YTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLL 120 Query: 121 SDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYK 180 SDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYK Sbjct: 121 SDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYK 180 Query: 181 STKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPL 240 STKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPL Sbjct: 181 STKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPL 240 Query: 241 ITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR 280 ITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR Sbjct: 241 ITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR 280 >4AXH-A nrPDB Length = 668 Score = 29.3 bits (64), Expect = 1.9, Method: Compositional matrix adjust. Identities = 10/32 (31%), Positives = 23/32 (71%) Query: 198 LKKVLSSKRNLHSFLDSALLNLMDKNVIYHNV 229 ++ L+ +R++++FL++ LNLM+K + H + Sbjct: 365 VRTYLADQRDMYAFLNNRALNLMNKGLTLHEI 396 >6I9E-A nrPDB Length = 409 Score = 28.9 bits (63), Expect = 2.3, Method: Compositional matrix adjust. Identities = 18/79 (22%), Positives = 38/79 (48%), Gaps = 6/79 (7%) Query: 128 RDRIESTT-----KPISQ-TSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYKS 181 RD I++ T +P++ G ++ ++FV+++ +Y GWV+ T++ + Y+ Sbjct: 218 RDLIKAATYFPDRRPVAIIVGPGFDEVLADNTFVQKYVEYEKGWVVGQNTVQPPREVYRQ 277 Query: 182 TKLPKPKRKNTNRIVALKK 200 L KR ++ K Sbjct: 278 AALDIFKRYTGLEVMVYDK 296 >3QJ4-A nrPDB Length = 342 Score = 26.9 bits (58), Expect = 8.8, Method: Compositional matrix adjust. Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 2/52 (3%) Query: 12 TYNPDIYFTSLDPQARRYTSKKINKQGTISTSRP--VKRINYSLADLEARLY 61 T NP I F S+D + R S +I I T+ P V + +S+ D++ ++ Sbjct: 216 TSNPCIRFVSIDNKKRNIESSEIGPSLVIHTTVPFGVTYLEHSIEDVQELVF 267 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6GEJ-S nrPDB 582 e-167 Sequences not found previously or not previously below threshold: 4AXH-A nrPDB 30 1.0 6EHL-A nrPDB 29 2.5 3QJ4-A nrPDB 29 2.9 4KNS-A nrPDB 28 4.4 3QML-C nrPDB 28 4.5 4F52-A nrPDB 28 5.4 3RTR-A nrPDB 28 5.4 1U6G-A nrPDB 28 5.4 CONVERGED! >6GEJ-S nrPDB Length = 280 Score = 582 bits (1500), Expect = e-167, Method: Composition-based stats. Identities = 280/280 (100%), Positives = 280/280 (100%) Query: 1 MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARL 60 MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARL Sbjct: 1 MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARL 60 Query: 61 YTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLL 120 YTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLL Sbjct: 61 YTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLL 120 Query: 121 SDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYK 180 SDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYK Sbjct: 121 SDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYK 180 Query: 181 STKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPL 240 STKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPL Sbjct: 181 STKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPL 240 Query: 241 ITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR 280 ITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR Sbjct: 241 ITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR 280 >4AXH-A nrPDB Length = 668 Score = 30.1 bits (66), Expect = 1.0, Method: Composition-based stats. Identities = 10/32 (31%), Positives = 23/32 (71%) Query: 198 LKKVLSSKRNLHSFLDSALLNLMDKNVIYHNV 229 ++ L+ +R++++FL++ LNLM+K + H + Sbjct: 365 VRTYLADQRDMYAFLNNRALNLMNKGLTLHEI 396 >6EHL-A nrPDB Length = 739 Score = 28.9 bits (63), Expect = 2.5, Method: Composition-based stats. Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 9/81 (11%) Query: 23 DPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARLYTSRSEGDGNSISR-QDDRNSK 81 + Q R TS N+ T++ PV R + +L + + D +R QD N++ Sbjct: 590 EEQDRDGTS---NRTPTVAPPAPVYRDHSEKKELPQ---DEQQDQDHTQEARNQDSDNTQ 643 Query: 82 NSHSFEERYTQQEILQSDRRF 102 + HSFEE Y + IL+S F Sbjct: 644 SEHSFEEMY--RHILRSQGPF 662 >3QJ4-A nrPDB Length = 342 Score = 28.5 bits (62), Expect = 2.9, Method: Composition-based stats. Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 2/52 (3%) Query: 12 TYNPDIYFTSLDPQARRYTSKKINKQGTISTSRP--VKRINYSLADLEARLY 61 T NP I F S+D + R S +I I T+ P V + +S+ D++ ++ Sbjct: 216 TSNPCIRFVSIDNKKRNIESSEIGPSLVIHTTVPFGVTYLEHSIEDVQELVF 267 >4KNS-A nrPDB Length = 285 Score = 28.1 bits (61), Expect = 4.4, Method: Composition-based stats. Identities = 11/33 (33%), Positives = 21/33 (63%) Query: 65 SEGDGNSISRQDDRNSKNSHSFEERYTQQEILQ 97 +EGD + +D+NSKNSHS + + ++++ Sbjct: 40 TEGDTVEFTLINDKNSKNSHSMDFHAARLDVVE 72 >3QML-C nrPDB Length = 315 Score = 28.1 bits (61), Expect = 4.5, Method: Composition-based stats. Identities = 23/70 (32%), Positives = 33/70 (47%), Gaps = 7/70 (10%) Query: 169 PETLREIQLSYKSTKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHN 228 P TLRE+ ST++ +N +V + S N S + +AL NL D N N Sbjct: 83 PLTLREL-----STRVITSCLRNNPPVVEF--INESFPNFKSKIMAALSNLNDSNHRSSN 135 Query: 229 VYNKRYFKVL 238 + KRY +L Sbjct: 136 ILIKRYLSIL 145 >4F52-A nrPDB Length = 282 Score = 27.8 bits (60), Expect = 5.4, Method: Composition-based stats. Identities = 34/134 (25%), Positives = 56/134 (41%), Gaps = 17/134 (12%) Query: 60 LYTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTL 119 Y SR G + Q + ++ F+ RYT Q ++ NTE+ + Sbjct: 161 FYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQ---- 216 Query: 120 LSDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSY 179 L+D T + D + + + ++ L L ++ V E LKP+TL ++ L Y Sbjct: 217 LTDSTQIKMDILAQVLQILLKSK--LLVLEDENANVDEVE-------LKPDTLIKLYLGY 267 Query: 180 KSTKLPKPKRKNTN 193 K+ KL R N N Sbjct: 268 KNKKL----RVNIN 277 >3RTR-A nrPDB Length = 368 Score = 27.8 bits (60), Expect = 5.4, Method: Composition-based stats. Identities = 34/134 (25%), Positives = 56/134 (41%), Gaps = 17/134 (12%) Query: 60 LYTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTL 119 Y SR G + Q + ++ F+ RYT Q ++ NTE+ + Sbjct: 161 FYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQ---- 216 Query: 120 LSDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSY 179 L+D T + D + + + ++ L L ++ V E LKP+TL ++ L Y Sbjct: 217 LTDSTQIKMDILAQVLQILLKSK--LLVLEDENANVDEVE-------LKPDTLIKLYLGY 267 Query: 180 KSTKLPKPKRKNTN 193 K+ KL R N N Sbjct: 268 KNKKL----RVNIN 277 >1U6G-A nrPDB Length = 776 Score = 27.8 bits (60), Expect = 5.4, Method: Composition-based stats. Identities = 34/134 (25%), Positives = 56/134 (41%), Gaps = 17/134 (12%) Query: 60 LYTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTL 119 Y SR G + Q + ++ F+ RYT Q ++ NTE+ + Sbjct: 569 FYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQ---- 624 Query: 120 LSDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSY 179 L+D T + D + + + ++ L L ++ V E LKP+TL ++ L Y Sbjct: 625 LTDSTQIKMDILAQVLQILLKSK--LLVLEDENANVDEVE-------LKPDTLIKLYLGY 675 Query: 180 KSTKLPKPKRKNTN 193 K+ KL R N N Sbjct: 676 KNKKL----RVNIN 685 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.131 0.375 Lambda K H 0.267 0.0405 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,896,964 Number of Sequences: 36641 Number of extensions: 423582 Number of successful extensions: 1019 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 1012 Number of HSP's gapped (non-prelim): 25 length of query: 280 length of database: 10,336,785 effective HSP length: 96 effective length of query: 184 effective length of database: 6,819,249 effective search space: 1254741816 effective search space used: 1254741816 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 58 (27.0 bits)