BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q03096 (181 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2M9V-A nrPDB 349 4e-97 1JNF-A nrPDB 27 3.3 3V0J-A nrPDB 27 3.5 4F2A-A nrPDB 27 6.6 >2M9V-A nrPDB Length = 170 Score = 349 bits (895), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 168/169 (99%), Positives = 168/169 (99%) Query: 13 TDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKF 72 TDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKF Sbjct: 2 TDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKF 61 Query: 73 YNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTN 132 YNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTN Sbjct: 62 YNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTN 121 Query: 133 SRAMSHFKICLSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL 181 SRAMSHFKI LSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL Sbjct: 122 SRAMSHFKISLSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL 170 >1JNF-A nrPDB Length = 676 Score = 27.3 bits (59), Expect = 3.3, Method: Composition-based stats. Identities = 13/55 (23%), Positives = 27/55 (49%) Query: 85 RDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTNSRAMSHF 139 RD + ++ +++ V +E+ H R+ S + G DL++ ++A HF Sbjct: 220 RDQYELLCLDNTRKPVDEYEQCHLARVPSHAVVARSVDGKEDLIWELLNQAQEHF 274 >3V0J-A nrPDB Length = 334 Score = 27.3 bits (59), Expect = 3.5, Method: Compositional matrix adjust. Identities = 12/48 (25%), Positives = 29/48 (60%), Gaps = 3/48 (6%) Query: 91 ILVEFSQ--ECVSNFERTHNCRITSETTNCLMIIGDADLVYVTNSRAM 136 +L +F++ C ++ T +C +T E NC ++ GD + +++ S+++ Sbjct: 230 LLCKFAEGYNCALQYDATDDC-VTCEVKNCPVLAGDIKVRFMSTSKSL 276 >4F2A-A nrPDB Length = 476 Score = 26.6 bits (57), Expect = 6.6, Method: Compositional matrix adjust. Identities = 12/27 (44%), Positives = 15/27 (55%) Query: 36 HPSGHVIPSLTKQDLALPHMSPTILTN 62 H GH I +DL LP SPT+L + Sbjct: 231 HHKGHFIYKDVSEDLPLPTFSPTLLGD 257 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2M9V-A nrPDB 331 1e-91 Sequences not found previously or not previously below threshold: 3V0J-A nrPDB 29 1.2 1JNF-A nrPDB 28 2.8 4F2A-A nrPDB 28 3.4 CONVERGED! >2M9V-A nrPDB Length = 170 Score = 331 bits (848), Expect = 1e-91, Method: Composition-based stats. Identities = 168/169 (99%), Positives = 168/169 (99%) Query: 13 TDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKF 72 TDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKF Sbjct: 2 TDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKF 61 Query: 73 YNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTN 132 YNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTN Sbjct: 62 YNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTN 121 Query: 133 SRAMSHFKICLSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL 181 SRAMSHFKI LSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL Sbjct: 122 SRAMSHFKISLSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL 170 >3V0J-A nrPDB Length = 334 Score = 28.8 bits (63), Expect = 1.2, Method: Composition-based stats. Identities = 15/60 (25%), Positives = 29/60 (48%), Gaps = 9/60 (15%) Query: 86 DSSHQILVEFSQECVSNFERTHNCR---------ITSETTNCLMIIGDADLVYVTNSRAM 136 D S QI+ E + + F +NC +T E NC ++ GD + +++ S+++ Sbjct: 217 DLSMQIVSERQEVLLCKFAEGYNCALQYDATDDCVTCEVKNCPVLAGDIKVRFMSTSKSL 276 >1JNF-A nrPDB Length = 676 Score = 27.7 bits (60), Expect = 2.8, Method: Composition-based stats. Identities = 13/55 (23%), Positives = 27/55 (49%) Query: 85 RDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTNSRAMSHF 139 RD + ++ +++ V +E+ H R+ S + G DL++ ++A HF Sbjct: 220 RDQYELLCLDNTRKPVDEYEQCHLARVPSHAVVARSVDGKEDLIWELLNQAQEHF 274 >4F2A-A nrPDB Length = 476 Score = 27.7 bits (60), Expect = 3.4, Method: Composition-based stats. Identities = 12/27 (44%), Positives = 15/27 (55%) Query: 34 QYHPSGHVIPSLTKQDLALPHMSPTIL 60 + H GH I +DL LP SPT+L Sbjct: 229 ESHHKGHFIYKDVSEDLPLPTFSPTLL 255 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.139 0.436 Lambda K H 0.267 0.0429 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,662,851 Number of Sequences: 36641 Number of extensions: 307163 Number of successful extensions: 603 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 597 Number of HSP's gapped (non-prelim): 8 length of query: 181 length of database: 10,336,785 effective HSP length: 91 effective length of query: 90 effective length of database: 7,002,454 effective search space: 630220860 effective search space used: 630220860 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 56 (26.1 bits)