BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q03067 (99 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3MHH-C nrPDB 204 1e-53 4ZUX-Y nrPDB 30 0.30 3THY-A nrPDB 27 1.6 6MKG-A nrPDB 25 6.0 3ZVH-B nrPDB 25 6.7 >3MHH-C nrPDB Length = 99 Score = 204 bits (518), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 99/99 (100%), Positives = 99/99 (100%) Query: 1 MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDING 60 MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDING Sbjct: 1 MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDING 60 Query: 61 LQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR 99 LQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR Sbjct: 61 LQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR 99 >4ZUX-Y nrPDB Length = 104 Score = 29.6 bits (65), Expect = 0.30, Method: Compositional matrix adjust. Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 3/42 (7%) Query: 49 YFDPNGSLDINGLQKQQESSQYIHCENCGRDVSANRLAAHLQ 90 YF+PN L + L K QY CE CG+ ++ + HL+ Sbjct: 57 YFNPNAQLIEDPLDK---PIQYRVCEKCGKPLALTAIVDHLE 95 >3THY-A nrPDB Length = 934 Score = 27.3 bits (59), Expect = 1.6, Method: Composition-based stats. Identities = 12/28 (42%), Positives = 19/28 (67%), Gaps = 1/28 (3%) Query: 18 NLLTTLIQDIVARETTQQQLLKTRYPDL 45 NL+ ++D R+T Q+ LL+ R+PDL Sbjct: 361 NLVEAFVEDAELRQTLQEDLLR-RFPDL 387 >6MKG-A nrPDB Length = 678 Score = 25.4 bits (54), Expect = 6.0, Method: Composition-based stats. Identities = 11/18 (61%), Positives = 14/18 (77%) Query: 51 DPNGSLDINGLQKQQESS 68 DPN L INGL+ Q++SS Sbjct: 439 DPNEVLTINGLKWQKDSS 456 >3ZVH-B nrPDB Length = 438 Score = 25.0 bits (53), Expect = 6.7, Method: Composition-based stats. Identities = 18/61 (29%), Positives = 27/61 (44%), Gaps = 4/61 (6%) Query: 26 DIVARETTQQQLLKTRYPDLRSYYFDPNGSLDINGLQKQQESSQYIHCENCGRDVSANRL 85 DI A Q L + P ++ G +D+ QKQ E+ + + E GR V A + Sbjct: 250 DIKAMADYIQTLAEAAKP----FHLRIEGPMDVEDRQKQMEAMRDLRAELDGRGVDAELV 305 Query: 86 A 86 A Sbjct: 306 A 306 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3MHH-C nrPDB 195 3e-51 Sequences not found previously or not previously below threshold: 4ZUX-Y nrPDB 30 0.18 3THY-A nrPDB 29 0.74 6MKG-A nrPDB 26 3.6 3ZVH-B nrPDB 25 6.3 5W1T-M nrPDB 25 7.0 1WRB-A nrPDB 25 8.5 CONVERGED! >3MHH-C nrPDB Length = 99 Score = 195 bits (496), Expect = 3e-51, Method: Composition-based stats. Identities = 99/99 (100%), Positives = 99/99 (100%) Query: 1 MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDING 60 MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDING Sbjct: 1 MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDING 60 Query: 61 LQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR 99 LQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR Sbjct: 61 LQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR 99 >4ZUX-Y nrPDB Length = 104 Score = 30.4 bits (67), Expect = 0.18, Method: Composition-based stats. Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 3/42 (7%) Query: 49 YFDPNGSLDINGLQKQQESSQYIHCENCGRDVSANRLAAHLQ 90 YF+PN L + L K QY CE CG+ ++ + HL+ Sbjct: 57 YFNPNAQLIEDPLDK---PIQYRVCEKCGKPLALTAIVDHLE 95 >3THY-A nrPDB Length = 934 Score = 28.5 bits (62), Expect = 0.74, Method: Composition-based stats. Identities = 12/28 (42%), Positives = 19/28 (67%), Gaps = 1/28 (3%) Query: 18 NLLTTLIQDIVARETTQQQLLKTRYPDL 45 NL+ ++D R+T Q+ LL+ R+PDL Sbjct: 361 NLVEAFVEDAELRQTLQEDLLR-RFPDL 387 >6MKG-A nrPDB Length = 678 Score = 26.2 bits (56), Expect = 3.6, Method: Composition-based stats. Identities = 11/18 (61%), Positives = 14/18 (77%) Query: 51 DPNGSLDINGLQKQQESS 68 DPN L INGL+ Q++SS Sbjct: 439 DPNEVLTINGLKWQKDSS 456 >3ZVH-B nrPDB Length = 438 Score = 25.4 bits (54), Expect = 6.3, Method: Composition-based stats. Identities = 18/61 (29%), Positives = 27/61 (44%), Gaps = 4/61 (6%) Query: 26 DIVARETTQQQLLKTRYPDLRSYYFDPNGSLDINGLQKQQESSQYIHCENCGRDVSANRL 85 DI A Q L + P ++ G +D+ QKQ E+ + + E GR V A + Sbjct: 250 DIKAMADYIQTLAEAAKP----FHLRIEGPMDVEDRQKQMEAMRDLRAELDGRGVDAELV 305 Query: 86 A 86 A Sbjct: 306 A 306 >5W1T-M nrPDB Length = 151 Score = 25.0 bits (53), Expect = 7.0, Method: Composition-based stats. Identities = 9/25 (36%), Positives = 15/25 (60%) Query: 63 KQQESSQYIHCENCGRDVSANRLAA 87 K+ E + +CE+CG ++ RL A Sbjct: 104 KKVEDEDFGYCESCGVEIGIRRLEA 128 >1WRB-A nrPDB Length = 253 Score = 24.7 bits (52), Expect = 8.5, Method: Composition-based stats. Identities = 16/58 (27%), Positives = 25/58 (43%), Gaps = 2/58 (3%) Query: 19 LLTTLIQDIVARETTQQQLLKTRYPDLRSYYFDPNGSLDINGLQKQQESSQYIHCENC 76 L +I +V ++ QQ+ KT YP + P L I L + Q+ S +C Sbjct: 77 FLIPIINHLVCQDLNQQRYSKTAYP--KCLILAPTRELAIQILSESQKFSLNTPLRSC 132 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.132 0.375 Lambda K H 0.267 0.0405 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,604,719 Number of Sequences: 36641 Number of extensions: 121608 Number of successful extensions: 392 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 384 Number of HSP's gapped (non-prelim): 14 length of query: 99 length of database: 10,336,785 effective HSP length: 67 effective length of query: 32 effective length of database: 7,881,838 effective search space: 252218816 effective search space used: 252218816 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.7 bits)