BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q02890 (363 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1X3Z-A nrPDB 699 0.0 2QSG-A nrPDB 33 0.14 3TLP-A nrPDB 31 0.94 4KBL-A nrPDB 29 2.8 5UDH-A nrPDB 29 2.9 >1X3Z-A nrPDB Length = 335 Score = 699 bits (1804), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/335 (100%), Positives = 335/335 (100%) Query: 8 NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYD 67 NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYD Sbjct: 1 NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYD 60 Query: 68 NPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKP 127 NPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKP Sbjct: 61 NPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKP 120 Query: 128 DCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETR 187 DCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETR Sbjct: 121 DCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETR 180 Query: 188 KGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIY 247 KGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIY Sbjct: 181 KGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIY 240 Query: 248 SINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD 307 SINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD Sbjct: 241 SINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD 300 Query: 308 EIYQLACRDEQEQIELIRGKTQETKSESVSAASKS 342 EIYQLACRDEQEQIELIRGKTQETKSESVSAASKS Sbjct: 301 EIYQLACRDEQEQIELIRGKTQETKSESVSAASKS 335 >2QSG-A nrPDB Length = 533 Score = 33.5 bits (75), Expect = 0.14, Method: Compositional matrix adjust. Identities = 29/119 (24%), Positives = 53/119 (44%), Gaps = 17/119 (14%) Query: 220 WCEYFSNFLNRWVHVDSCE-QSFDQPYIYSINWNKKMS--------YCIAFGKD-GVVDV 269 WCE + F +W+ VD ++ +Q ++S K ++ Y IA+ + G DV Sbjct: 217 WCEVWDKFSKKWITVDPVNLKTIEQVRLHSKLAPKGVACCERNMLRYVIAYDRKYGCRDV 276 Query: 270 SKRY-------ILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDDEIYQLACRDEQEQI 321 ++RY + + + +D E+ + + + R R ++D E RDE E I Sbjct: 277 TRRYAQWMNSKVRKRRITKDDFGEKWFRKVITALHHRKRTKIDDYEDQYFFQRDESEGI 335 >3TLP-A nrPDB Length = 150 Score = 30.8 bits (68), Expect = 0.94, Method: Compositional matrix adjust. Identities = 23/104 (22%), Positives = 47/104 (45%), Gaps = 25/104 (24%) Query: 57 GQSQHLCTVY-------DNPSWHSIVLETLDLDLIYKNV--DKEFAKDG----------- 96 G + LC ++ D P ++ I+LE +DL +I N+ DK ++G Sbjct: 43 GSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRN 102 Query: 97 ----HAEGENIYTD-YLVKELLRYFKQDFFKWCNKPDCNHCGQN 135 + EG +Y D +++++LL+ +++ + D +N Sbjct: 103 ARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPAEN 146 >4KBL-A nrPDB Length = 559 Score = 29.3 bits (64), Expect = 2.8, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 2/39 (5%) Query: 120 FFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNCG 158 KWC PDC+H + + P+ + G + FNCG Sbjct: 274 LLKWCPAPDCHHVVKVQYPDAKPVRCKC--GRQFCFNCG 310 >5UDH-A nrPDB Length = 470 Score = 29.3 bits (64), Expect = 2.9, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 2/39 (5%) Query: 120 FFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNCG 158 KWC PDC+H + + P+ + G + FNCG Sbjct: 185 LLKWCPAPDCHHVVKVQYPDAKPVRCKC--GRQFCFNCG 221 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1X3Z-A nrPDB 741 0.0 Sequences not found previously or not previously below threshold: 2QSG-A nrPDB 37 0.018 3TLP-A nrPDB 31 1.0 4KBL-A nrPDB 30 1.4 5UDH-A nrPDB 30 1.5 4PSX-A nrPDB 28 9.0 CONVERGED! >1X3Z-A nrPDB Length = 335 Score = 741 bits (1913), Expect = 0.0, Method: Composition-based stats. Identities = 335/335 (100%), Positives = 335/335 (100%) Query: 8 NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYD 67 NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYD Sbjct: 1 NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYD 60 Query: 68 NPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKP 127 NPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKP Sbjct: 61 NPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKP 120 Query: 128 DCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETR 187 DCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETR Sbjct: 121 DCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETR 180 Query: 188 KGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIY 247 KGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIY Sbjct: 181 KGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIY 240 Query: 248 SINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD 307 SINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD Sbjct: 241 SINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD 300 Query: 308 EIYQLACRDEQEQIELIRGKTQETKSESVSAASKS 342 EIYQLACRDEQEQIELIRGKTQETKSESVSAASKS Sbjct: 301 EIYQLACRDEQEQIELIRGKTQETKSESVSAASKS 335 >2QSG-A nrPDB Length = 533 Score = 36.6 bits (83), Expect = 0.018, Method: Composition-based stats. Identities = 33/119 (27%), Positives = 58/119 (48%), Gaps = 17/119 (14%) Query: 220 WCEYFSNFLNRWVHVDSCE-QSFDQPYIYSINWNKKMSYC--------IAFG-KDGVVDV 269 WCE + F +W+ VD ++ +Q ++S K ++ C IA+ K G DV Sbjct: 217 WCEVWDKFSKKWITVDPVNLKTIEQVRLHSKLAPKGVACCERNMLRYVIAYDRKYGCRDV 276 Query: 270 SKRYI--LQNELPRDQIKEEDL--KFLCQFIT---KRLRYSLNDDEIYQLACRDEQEQI 321 ++RY + +++ + +I ++D K+ + IT R R ++D E RDE E I Sbjct: 277 TRRYAQWMNSKVRKRRITKDDFGEKWFRKVITALHHRKRTKIDDYEDQYFFQRDESEGI 335 >3TLP-A nrPDB Length = 150 Score = 30.8 bits (68), Expect = 1.0, Method: Composition-based stats. Identities = 21/89 (23%), Positives = 43/89 (48%), Gaps = 25/89 (28%) Query: 57 GQSQHLCTVY-------DNPSWHSIVLETLDLDLIYKNV--DKEFAKDG----------- 96 G + LC ++ D P ++ I+LE +DL +I N+ DK ++G Sbjct: 43 GSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRN 102 Query: 97 ----HAEGENIYTD-YLVKELLRYFKQDF 120 + EG +Y D +++++LL+ +++ Sbjct: 103 ARHYNEEGSQVYNDAHILEKLLKEKRKEL 131 >4KBL-A nrPDB Length = 559 Score = 30.4 bits (67), Expect = 1.4, Method: Composition-based stats. Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 2/39 (5%) Query: 120 FFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNCG 158 KWC PDC+H + + P+ + G + FNCG Sbjct: 274 LLKWCPAPDCHHVVKVQYPDAKPVRCKC--GRQFCFNCG 310 >5UDH-A nrPDB Length = 470 Score = 30.0 bits (66), Expect = 1.5, Method: Composition-based stats. Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 2/39 (5%) Query: 120 FFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNCG 158 KWC PDC+H + + P+ + G + FNCG Sbjct: 185 LLKWCPAPDCHHVVKVQYPDAKPVRCKC--GRQFCFNCG 221 >4PSX-A nrPDB Length = 320 Score = 27.7 bits (60), Expect = 9.0, Method: Composition-based stats. Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 10/65 (15%) Query: 243 QPYIYSINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQ-IKEEDLKFLCQFITKRLR 301 +PY+ + Y G D +VDV K+ L + LP+D I ++ K++ F +R Sbjct: 64 KPYV-------NVKYSAKLGDDNIVDVEKK--LLSFLPKDDVIVRDEAKWVDCFAEERKT 114 Query: 302 YSLND 306 ++L+D Sbjct: 115 HNLSD 119 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.136 0.428 Lambda K H 0.267 0.0434 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,636,767 Number of Sequences: 36641 Number of extensions: 758633 Number of successful extensions: 1513 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 1511 Number of HSP's gapped (non-prelim): 16 length of query: 363 length of database: 10,336,785 effective HSP length: 98 effective length of query: 265 effective length of database: 6,745,967 effective search space: 1787681255 effective search space used: 1787681255 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 60 (27.6 bits)