BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q02775 (382 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5YLZ-V nrPDB 786 0.0 6QDV-c nrPDB 60 1e-09 3MWY-W nrPDB 32 0.32 2IWK-A nrPDB 29 2.9 3PLN-A nrPDB 29 4.3 5O2U-A nrPDB 29 4.4 4B56-A nrPDB 28 5.0 4MVL-A nrPDB 28 5.5 >5YLZ-V nrPDB Length = 382 Score = 786 bits (2029), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/382 (100%), Positives = 382/382 (100%) Query: 1 MNNNSRNNENRSTINRNKRQLQQAKEKNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDD 60 MNNNSRNNENRSTINRNKRQLQQAKEKNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDD Sbjct: 1 MNNNSRNNENRSTINRNKRQLQQAKEKNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDD 60 Query: 61 TSTAEGGEKSDYLVHHRQKAKGGALDIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLS 120 TSTAEGGEKSDYLVHHRQKAKGGALDIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLS Sbjct: 61 TSTAEGGEKSDYLVHHRQKAKGGALDIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLS 120 Query: 121 FCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGK 180 FCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGK Sbjct: 121 FCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGK 180 Query: 181 EYNELISKWERDKRNKIKGKDKSQTDETLWDTDEEIELMKLELYKDSVGSLKKDDADNSQ 240 EYNELISKWERDKRNKIKGKDKSQTDETLWDTDEEIELMKLELYKDSVGSLKKDDADNSQ Sbjct: 181 EYNELISKWERDKRNKIKGKDKSQTDETLWDTDEEIELMKLELYKDSVGSLKKDDADNSQ 240 Query: 241 LYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLN 300 LYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLN Sbjct: 241 LYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLN 300 Query: 301 ELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLE 360 ELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLE Sbjct: 301 ELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLE 360 Query: 361 ARKVDGTKQSEEQRNHLKDLYG 382 ARKVDGTKQSEEQRNHLKDLYG Sbjct: 361 ARKVDGTKQSEEQRNHLKDLYG 382 >6QDV-c nrPDB Length = 586 Score = 60.5 bits (145), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 86/352 (24%), Positives = 146/352 (41%), Gaps = 90/352 (25%) Query: 27 KNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDDTSTAEGGEKSDYLVHHR-QKAKGGAL 85 K+ N HIP+YI + PWY + K+P L H R Q K Sbjct: 57 KDINPHIPQYISSVPWYIDPS------KRPT---------------LKHQRPQPEKQKQF 95 Query: 86 DIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLSFCRNCGEAGHKEKDCMEKPRKM--- 142 ++S G+K+ S+ + C NCG HK+KDC E+PR++ Sbjct: 96 ---SSSGEWYKRGVKEN---------SIITKYRKGACENCGAMTHKKKDCFERPRRVGAK 143 Query: 143 ---QKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGKEYNELISKWERD------- 192 + PD + Q ++ D+D ++DRW GY+ +E+ +++ ++ + Sbjct: 144 FTGTNIAPDEHVQPQ---LMF-----DYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTL 195 Query: 193 KRNKIK-----GK--DKSQTDETLWDTDEEIELMKLEL---------YKDSVGSLKKDDA 236 K K++ GK +++ + + W +E M+ + Y D + ++ Sbjct: 196 KAQKLQEELASGKLVEQANSPKHQWGEEEPNSQMEKDHNSEDEDEDKYADDIDMPGQNFD 255 Query: 237 DNSQLYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAV----DEKSKM---FR 289 ++ + R+RED A YL +++ + YDPK+R + DE S F Sbjct: 256 SKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFV 315 Query: 290 RHLTGEGLKLNELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKK 341 R+ TG+ + + + FA E DK H L A+PTK E L K Sbjct: 316 RY-TGDTISMAQTQLFAW----------EAYDKGSEVH-LQADPTKLELLYK 355 >3MWY-W nrPDB Length = 800 Score = 32.3 bits (72), Expect = 0.32, Method: Compositional matrix adjust. Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 5/51 (9%) Query: 115 DSHSLSFCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDD 165 D H + N H+ K +E+ + ++K VPDLN+ K N L++ TD+ Sbjct: 36 DFHGIDIVIN-----HRLKTSLEEGKVLEKTVPDLNNCKENYEFLIKWTDE 81 >2IWK-A nrPDB Length = 642 Score = 29.3 bits (64), Expect = 2.9, Method: Compositional matrix adjust. Identities = 17/50 (34%), Positives = 26/50 (52%) Query: 305 FARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIV 354 F+ S+ EMG+ E K ++ HV+V N + E K + EE K+V Sbjct: 257 FSTSYNSEMGMTLEEMTKSEMDHVVVFNIAEIEKAIKAGQYEEINGVKVV 306 >3PLN-A nrPDB Length = 424 Score = 28.9 bits (63), Expect = 4.3, Method: Compositional matrix adjust. Identities = 27/107 (25%), Positives = 50/107 (46%), Gaps = 21/107 (19%) Query: 259 INSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLNELNQFARSHAKE---MGI 315 I +T ++YDPK+ + T T+ AV R +T E+N A K +G Sbjct: 114 IIATPTDYDPKTNYFNTSTVEAV-------IRDVT-------EINPNAVMIIKSTIPVGF 159 Query: 316 RDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLEAR 362 +I+++ + +V+ + E+L++ R + P + IG+ AR Sbjct: 160 TRDIKERLGIDNVIFSP----EFLREGRALYDNLHPSRIVIGERSAR 202 >5O2U-A nrPDB Length = 500 Score = 28.9 bits (63), Expect = 4.4, Method: Compositional matrix adjust. Identities = 15/36 (41%), Positives = 19/36 (52%), Gaps = 1/36 (2%) Query: 106 IRPQKMSVRDSHSLSFCRNCGEAGHKEKDCMEKPRK 141 I QK + R+ C NCG+ GH K+C PRK Sbjct: 376 IMIQKGNFRNQRKTVKCFNCGKEGHIAKNC-RAPRK 410 Score = 27.7 bits (60), Expect = 8.6, Method: Compositional matrix adjust. Identities = 9/17 (52%), Positives = 12/17 (70%) Query: 122 CRNCGEAGHKEKDCMEK 138 C CG+ GH+ KDC E+ Sbjct: 413 CWKCGKEGHQMKDCTER 429 >4B56-A nrPDB Length = 820 Score = 28.5 bits (62), Expect = 5.0, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 25/59 (42%), Gaps = 4/59 (6%) Query: 255 YLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLNELNQFARSHAKEM 313 YL D+N+ + Y P +RL T+ V E F + L E NQ R + K Sbjct: 336 YLGDVNNVKVVYGPAARLRPTD----VPETYYSFNYEALAKNLSCREPNQHFRPYLKPF 390 >4MVL-A nrPDB Length = 188 Score = 28.5 bits (62), Expect = 5.5, Method: Compositional matrix adjust. Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 5/48 (10%) Query: 231 LKKDDADNSQLYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETL 278 L ++D D ++Y T L+EDK+ N T +D K LYK T Sbjct: 41 LLREDKDPLKMYATIYELKEDKSY-----NVTSVGFDDKKCLYKIRTF 83 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5YLZ-V nrPDB 553 e-158 6QDV-c nrPDB 319 2e-87 Sequences not found previously or not previously below threshold: 5O2U-A nrPDB 32 0.46 5ECK-A nrPDB 29 2.3 CONVERGED! >5YLZ-V nrPDB Length = 382 Score = 553 bits (1424), Expect = e-158, Method: Composition-based stats. Identities = 382/382 (100%), Positives = 382/382 (100%) Query: 1 MNNNSRNNENRSTINRNKRQLQQAKEKNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDD 60 MNNNSRNNENRSTINRNKRQLQQAKEKNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDD Sbjct: 1 MNNNSRNNENRSTINRNKRQLQQAKEKNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDD 60 Query: 61 TSTAEGGEKSDYLVHHRQKAKGGALDIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLS 120 TSTAEGGEKSDYLVHHRQKAKGGALDIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLS Sbjct: 61 TSTAEGGEKSDYLVHHRQKAKGGALDIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLS 120 Query: 121 FCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGK 180 FCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGK Sbjct: 121 FCRNCGEAGHKEKDCMEKPRKMQKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGK 180 Query: 181 EYNELISKWERDKRNKIKGKDKSQTDETLWDTDEEIELMKLELYKDSVGSLKKDDADNSQ 240 EYNELISKWERDKRNKIKGKDKSQTDETLWDTDEEIELMKLELYKDSVGSLKKDDADNSQ Sbjct: 181 EYNELISKWERDKRNKIKGKDKSQTDETLWDTDEEIELMKLELYKDSVGSLKKDDADNSQ 240 Query: 241 LYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLN 300 LYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLN Sbjct: 241 LYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAVDEKSKMFRRHLTGEGLKLN 300 Query: 301 ELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLE 360 ELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLE Sbjct: 301 ELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKKKREQEETKQPKIVSIGDLE 360 Query: 361 ARKVDGTKQSEEQRNHLKDLYG 382 ARKVDGTKQSEEQRNHLKDLYG Sbjct: 361 ARKVDGTKQSEEQRNHLKDLYG 382 >6QDV-c nrPDB Length = 586 Score = 319 bits (816), Expect = 2e-87, Method: Composition-based stats. Identities = 91/369 (24%), Positives = 156/369 (42%), Gaps = 92/369 (24%) Query: 27 KNENIHIPRYIRNQPWYYKDTPKEQEGKKPGNDDTSTAEGGEKSDYLVHHR-QKAKGGAL 85 K+ N HIP+YI + PWY + K+P L H R Q K Sbjct: 57 KDINPHIPQYISSVPWYIDPS------KRPT---------------LKHQRPQPEKQKQF 95 Query: 86 DIDNNSEPKIGMGIKDEFKLIRPQKMSVRDSHSLSFCRNCGEAGHKEKDCMEKPRKM--- 142 ++S G+K+ S+ + C NCG HK+KDC E+PR++ Sbjct: 96 ---SSSGEWYKRGVKEN---------SIITKYRKGACENCGAMTHKKKDCFERPRRVGAK 143 Query: 143 ---QKLVPDLNSQKNNGTVLVRATDDDWDSRKDRWYGYSGKEYNELISKWERD------- 192 + PD + Q ++ D+D ++DRW GY+ +E+ +++ ++ + Sbjct: 144 FTGTNIAPDEHVQPQ---LMF-----DYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTL 195 Query: 193 KRNKIK-----GK--DKSQTDETLWDTDEEIELMKLEL---------YKDSVGSLKKDDA 236 K K++ GK +++ + + W +E M+ + Y D + ++ Sbjct: 196 KAQKLQEELASGKLVEQANSPKHQWGEEEPNSQMEKDHNSEDEDEDKYADDIDMPGQNFD 255 Query: 237 DNSQLYRTSTRLREDKAAYLNDINSTESNYDPKSRLYKTETLGAV----DEKSKM---FR 289 ++ + R+RED A YL +++ + YDPK+R + DE S F Sbjct: 256 SKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFV 315 Query: 290 RHLTGEGLKLNELNQFARSHAKEMGIRDEIEDKEKVQHVLVANPTKYEYLKK--KREQEE 347 R+ TG+ + + + FA E DK H L A+PTK E L K K ++E+ Sbjct: 316 RY-TGDTISMAQTQLFAW----------EAYDKGSEVH-LQADPTKLELLYKSFKVKKED 363 Query: 348 TKQPKIVSI 356 K+ + SI Sbjct: 364 FKEQQKESI 372 >5O2U-A nrPDB Length = 500 Score = 31.8 bits (70), Expect = 0.46, Method: Composition-based stats. Identities = 12/39 (30%), Positives = 18/39 (46%), Gaps = 1/39 (2%) Query: 118 SLSFCRNCGEAGHKEKDCMEKPRK-MQKLVPDLNSQKNN 155 C CG+ GH+ KDC E+ + K+ P + N Sbjct: 409 RKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGN 447 Score = 30.2 bits (66), Expect = 1.4, Method: Composition-based stats. Identities = 15/40 (37%), Positives = 19/40 (47%), Gaps = 1/40 (2%) Query: 102 EFKLIRPQKMSVRDSHSLSFCRNCGEAGHKEKDCMEKPRK 141 I QK + R+ C NCG+ GH K+C PRK Sbjct: 372 NPATIMIQKGNFRNQRKTVKCFNCGKEGHIAKNC-RAPRK 410 >5ECK-A nrPDB Length = 575 Score = 29.5 bits (64), Expect = 2.3, Method: Composition-based stats. Identities = 13/83 (15%), Positives = 27/83 (32%) Query: 214 EEIELMKLELYKDSVGSLKKDDADNSQLYRTSTRLREDKAAYLNDINSTESNYDPKSRLY 273 E++E + D + ++ + L+ A YL + + DP+ Sbjct: 3 EKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEAFK 62 Query: 274 KTETLGAVDEKSKMFRRHLTGEG 296 L E +R + G+ Sbjct: 63 SMVPLVTDVELEPYIKRMVDGDT 85 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.300 0.116 0.304 Lambda K H 0.267 0.0347 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,081,834 Number of Sequences: 36641 Number of extensions: 502283 Number of successful extensions: 1379 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 1334 Number of HSP's gapped (non-prelim): 56 length of query: 382 length of database: 10,336,785 effective HSP length: 99 effective length of query: 283 effective length of database: 6,709,326 effective search space: 1898739258 effective search space used: 1898739258 T: 11 A: 40 X1: 16 ( 6.9 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.3 bits) S2: 59 (27.6 bits)