BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P86242
         (143 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

5NTB-A nrPDB                                                          231   6e-62
6IW1-A nrPDB                                                           27   3.4  
5ESS-A nrPDB                                                           26   5.7  

>5NTB-A nrPDB
          Length = 110

 Score =  231 bits (590), Expect = 6e-62,   Method: Compositional matrix adjust.
 Identities = 110/110 (100%), Positives = 110/110 (100%)

Query: 34  DIPIGQKMTGKMTYYTDKGYGACGTPIDASSQDLVAIPAAWWTTPNPNNDPLCRGVSVEV 93
           DIPIGQKMTGKMTYYTDKGYGACGTPIDASSQDLVAIPAAWWTTPNPNNDPLCRGVSVEV
Sbjct: 1   DIPIGQKMTGKMTYYTDKGYGACGTPIDASSQDLVAIPAAWWTTPNPNNDPLCRGVSVEV 60

Query: 94  SYNGRTIRVPVRDKCPSCDRTHIDLSQAAFAKLAPLDRGVVNGITWKFVR 143
           SYNGRTIRVPVRDKCPSCDRTHIDLSQAAFAKLAPLDRGVVNGITWKFVR
Sbjct: 61  SYNGRTIRVPVRDKCPSCDRTHIDLSQAAFAKLAPLDRGVVNGITWKFVR 110


>6IW1-A nrPDB
          Length = 395

 Score = 26.9 bits (58), Expect = 3.4,   Method: Compositional matrix adjust.
 Identities = 10/25 (40%), Positives = 13/25 (52%)

Query: 92  EVSYNGRTIRVPVRDKCPSCDRTHI 116
           +V YN     V + DKCPS    H+
Sbjct: 58  KVCYNAVLTHVKINDKCPSTGEAHL 82


>5ESS-A nrPDB
          Length = 574

 Score = 26.2 bits (56), Expect = 5.7,   Method: Compositional matrix adjust.
 Identities = 15/32 (46%), Positives = 20/32 (62%), Gaps = 1/32 (3%)

Query: 11  RFAACALVAAATGI-TLAAGPASADIPIGQKM 41
           R A C ++AAATG  T  AGP   DIP+ + +
Sbjct: 174 RSATCRVLAAATGARTANAGPVHFDIPLREPL 205


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

5NTB-A nrPDB                                                          238   7e-64

Sequences not found previously or not previously below threshold:

5ESS-A nrPDB                                                           28   1.1  
6IW1-A nrPDB                                                           27   3.8  
5H4V-A nrPDB                                                           26   6.3  


CONVERGED!
>5NTB-A nrPDB
          Length = 110

 Score =  238 bits (607), Expect = 7e-64,   Method: Composition-based stats.
 Identities = 110/110 (100%), Positives = 110/110 (100%)

Query: 34  DIPIGQKMTGKMTYYTDKGYGACGTPIDASSQDLVAIPAAWWTTPNPNNDPLCRGVSVEV 93
           DIPIGQKMTGKMTYYTDKGYGACGTPIDASSQDLVAIPAAWWTTPNPNNDPLCRGVSVEV
Sbjct: 1   DIPIGQKMTGKMTYYTDKGYGACGTPIDASSQDLVAIPAAWWTTPNPNNDPLCRGVSVEV 60

Query: 94  SYNGRTIRVPVRDKCPSCDRTHIDLSQAAFAKLAPLDRGVVNGITWKFVR 143
           SYNGRTIRVPVRDKCPSCDRTHIDLSQAAFAKLAPLDRGVVNGITWKFVR
Sbjct: 61  SYNGRTIRVPVRDKCPSCDRTHIDLSQAAFAKLAPLDRGVVNGITWKFVR 110


>5ESS-A nrPDB
          Length = 574

 Score = 28.5 bits (62), Expect = 1.1,   Method: Composition-based stats.
 Identities = 15/32 (46%), Positives = 20/32 (62%), Gaps = 1/32 (3%)

Query: 11  RFAACALVAAATGITLA-AGPASADIPIGQKM 41
           R A C ++AAATG   A AGP   DIP+ + +
Sbjct: 174 RSATCRVLAAATGARTANAGPVHFDIPLREPL 205


>6IW1-A nrPDB
          Length = 395

 Score = 26.6 bits (57), Expect = 3.8,   Method: Composition-based stats.
 Identities = 10/25 (40%), Positives = 13/25 (52%)

Query: 92  EVSYNGRTIRVPVRDKCPSCDRTHI 116
           +V YN     V + DKCPS    H+
Sbjct: 58  KVCYNAVLTHVKINDKCPSTGEAHL 82


>5H4V-A nrPDB
          Length = 488

 Score = 25.8 bits (55), Expect = 6.3,   Method: Composition-based stats.
 Identities = 10/19 (52%), Positives = 14/19 (73%)

Query: 20  AATGITLAAGPASADIPIG 38
           A  G+ LAAGPA+AD+ + 
Sbjct: 349 AKLGVDLAAGPAAADVVVA 367


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.322    0.135    0.431 

Lambda     K      H
   0.267   0.0415    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,833,911
Number of Sequences: 36641
Number of extensions: 228552
Number of successful extensions: 495
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 485
Number of HSP's gapped (non-prelim): 14
length of query: 143
length of database: 10,336,785
effective HSP length: 88
effective length of query: 55
effective length of database: 7,112,377
effective search space: 391180735
effective search space used: 391180735
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)