BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P81451
         (68 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

6B8H-k nrPDB                                                          140   2e-34
5AMB-A nrPDB                                                           26   4.3  
5I68-A nrPDB                                                           26   4.5  
4LGB-B nrPDB                                                           25   7.3  

>6B8H-k nrPDB
          Length = 68

 Score =  140 bits (352), Expect = 2e-34,   Method: Compositional matrix adjust.
 Identities = 68/68 (100%), Positives = 68/68 (100%)

Query: 1  MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDIKTDNKDEEKFIENYLK 60
          MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDIKTDNKDEEKFIENYLK
Sbjct: 1  MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDIKTDNKDEEKFIENYLK 60

Query: 61 KHSEKQDA 68
          KHSEKQDA
Sbjct: 61 KHSEKQDA 68


>5AMB-A nrPDB
          Length = 629

 Score = 25.8 bits (55), Expect = 4.3,   Method: Composition-based stats.
 Identities = 9/26 (34%), Positives = 14/26 (53%)

Query: 33  PFKSAKPKTVDIKTDNKDEEKFIENY 58
           P     P+ +D+ TD  +  KF+E Y
Sbjct: 602 PLPDNYPEGIDLVTDEAEASKFVEEY 627


>5I68-A nrPDB
          Length = 663

 Score = 25.8 bits (55), Expect = 4.5,   Method: Composition-based stats.
 Identities = 11/22 (50%), Positives = 18/22 (81%), Gaps = 1/22 (4%)

Query: 43  DIKTDNKDEEKFIENYLKKHSE 64
           DI+ D +D+ K IENY+++H+E
Sbjct: 543 DIEYDEEDD-KAIENYIREHTE 563


>4LGB-B nrPDB
          Length = 341

 Score = 25.0 bits (53), Expect = 7.3,   Method: Composition-based stats.
 Identities = 12/23 (52%), Positives = 14/23 (60%)

Query: 35  KSAKPKTVDIKTDNKDEEKFIEN 57
           K A P +VD K D +DE   IEN
Sbjct: 185 KEAMPLSVDHKPDREDEYARIEN 207


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

6B8H-k nrPDB                                                          140   2e-34

Sequences not found previously or not previously below threshold:

5I68-A nrPDB                                                           27   1.7  
1YWO-A nrPDB                                                           27   1.8  
5AMB-A nrPDB                                                           27   2.9  


CONVERGED!
>6B8H-k nrPDB
          Length = 68

 Score =  140 bits (352), Expect = 2e-34,   Method: Composition-based stats.
 Identities = 68/68 (100%), Positives = 68/68 (100%)

Query: 1  MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDIKTDNKDEEKFIENYLK 60
          MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDIKTDNKDEEKFIENYLK
Sbjct: 1  MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDIKTDNKDEEKFIENYLK 60

Query: 61 KHSEKQDA 68
          KHSEKQDA
Sbjct: 61 KHSEKQDA 68


>5I68-A nrPDB
          Length = 663

 Score = 27.3 bits (59), Expect = 1.7,   Method: Composition-based stats.
 Identities = 11/22 (50%), Positives = 18/22 (81%), Gaps = 1/22 (4%)

Query: 43  DIKTDNKDEEKFIENYLKKHSE 64
           DI+ D +D+ K IENY+++H+E
Sbjct: 543 DIEYDEEDD-KAIENYIREHTE 563


>1YWO-A nrPDB
          Length = 64

 Score = 26.9 bits (58), Expect = 1.8,   Method: Composition-based stats.
 Identities = 13/35 (37%), Positives = 19/35 (54%)

Query: 34 FKSAKPKTVDIKTDNKDEEKFIENYLKKHSEKQDA 68
          FKSA     D K   +DE  F ++ + ++ EKQD 
Sbjct: 5  FKSAVKALFDYKAQREDELTFTKSAIIQNVEKQDG 39


>5AMB-A nrPDB
          Length = 629

 Score = 26.5 bits (57), Expect = 2.9,   Method: Composition-based stats.
 Identities = 9/26 (34%), Positives = 14/26 (53%)

Query: 33  PFKSAKPKTVDIKTDNKDEEKFIENY 58
           P     P+ +D+ TD  +  KF+E Y
Sbjct: 602 PLPDNYPEGIDLVTDEAEASKFVEEY 627


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.315    0.135    0.391 

Lambda     K      H
   0.267   0.0422    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,809,805
Number of Sequences: 36641
Number of extensions: 94332
Number of successful extensions: 188
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 176
Number of HSP's gapped (non-prelim): 13
length of query: 68
length of database: 10,336,785
effective HSP length: 39
effective length of query: 29
effective length of database: 8,907,786
effective search space: 258325794
effective search space used: 258325794
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 53 (25.0 bits)