BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P77335 (303 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6MRT-A nrPDB 611 e-175 3LWJ-A nrPDB 29 1.9 4K7C-A nrPDB 27 8.9 3M0D-C nrPDB 27 9.4 >6MRT-A nrPDB Length = 324 Score = 611 bits (1576), Expect = e-175, Method: Compositional matrix adjust. Identities = 302/303 (99%), Positives = 302/303 (99%) Query: 1 MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASV 60 MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASV Sbjct: 22 MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASV 81 Query: 61 LVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVL 120 LVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVL Sbjct: 82 LVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVL 141 Query: 121 DDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAG 180 DDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAG Sbjct: 142 DDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAG 201 Query: 181 AAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAK 240 AAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAK Sbjct: 202 AAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAK 261 Query: 241 LKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEV 300 LKLTTEI AIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEV Sbjct: 262 LKLTTEIVAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEV 321 Query: 301 PEV 303 PEV Sbjct: 322 PEV 324 >3LWJ-A nrPDB Length = 202 Score = 29.3 bits (64), Expect = 1.9, Method: Compositional matrix adjust. Identities = 15/43 (34%), Positives = 31/43 (72%), Gaps = 1/43 (2%) Query: 246 EIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEY 288 +I A+ E+ T T ++VD +D++ +LL++ AK++I++ +EY Sbjct: 37 DIIALSEVGTGT-FYNYFVDKEDILKNLLEDFAKQIISSISEY 78 >4K7C-A nrPDB Length = 446 Score = 27.3 bits (59), Expect = 8.9, Method: Compositional matrix adjust. Identities = 35/127 (27%), Positives = 52/127 (40%), Gaps = 19/127 (14%) Query: 161 EKSSYFQSQVDKI-------RKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPEL-- 211 EKS+YF V K RK A+ A G + ++ + G+V ++PE Sbjct: 106 EKSNYFYENVLKTADQPLDSRKVAFLLATPQQDGGQWDMLSALIEKYGIVPKSVMPETYS 165 Query: 212 ---KNKLKSVQNF-----FTTLSNTV--KQANKDIDAAKLKLTTEIAAIGEIKTETETTR 261 N+L + N TL V K ++ DI+AAK K+ E I T+ Sbjct: 166 SSKSNELNGLLNLKLRKDAVTLRKLVADKASDADIEAAKQKMLAEDYRILAYTLGNPPTK 225 Query: 262 FYVDYDD 268 F +Y D Sbjct: 226 FDFEYRD 232 >3M0D-C nrPDB Length = 65 Score = 27.3 bits (59), Expect = 9.4, Method: Compositional matrix adjust. Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 7/42 (16%) Query: 206 KLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAKLKLTTEI 247 KL+ EL+ KL+ +N L NK+++A+ L L T I Sbjct: 6 KLLAELEGKLRVFENIVAVL-------NKEVEASHLALATSI 40 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6MRT-A nrPDB 550 e-157 Sequences not found previously or not previously below threshold: 3LWJ-A nrPDB 31 0.71 4LCB-A nrPDB 28 6.8 4K7C-A nrPDB 28 7.1 3PYM-A nrPDB 27 9.6 CONVERGED! >6MRT-A nrPDB Length = 324 Score = 550 bits (1418), Expect = e-157, Method: Composition-based stats. Identities = 302/303 (99%), Positives = 302/303 (99%) Query: 1 MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASV 60 MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASV Sbjct: 22 MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASV 81 Query: 61 LVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVL 120 LVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVL Sbjct: 82 LVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILIKVL 141 Query: 121 DDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAG 180 DDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAG Sbjct: 142 DDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVDKIRKEAYAG 201 Query: 181 AAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAK 240 AAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAK Sbjct: 202 AAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTVKQANKDIDAAK 261 Query: 241 LKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEV 300 LKLTTEI AIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEV Sbjct: 262 LKLTTEIVAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEYQKRHGKKTLFEV 321 Query: 301 PEV 303 PEV Sbjct: 322 PEV 324 >3LWJ-A nrPDB Length = 202 Score = 30.7 bits (68), Expect = 0.71, Method: Composition-based stats. Identities = 15/43 (34%), Positives = 31/43 (72%), Gaps = 1/43 (2%) Query: 246 EIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNEY 288 +I A+ E+ T T ++VD +D++ +LL++ AK++I++ +EY Sbjct: 37 DIIALSEVGTGT-FYNYFVDKEDILKNLLEDFAKQIISSISEY 78 >4LCB-A nrPDB Length = 367 Score = 27.7 bits (60), Expect = 6.8, Method: Composition-based stats. Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 3/54 (5%) Query: 253 IKTETETTRFYVDYDDLMLSLLKEAAK---KMINTCNEYQKRHGKKTLFEVPEV 303 + E ++ +VD +M L EA K K+ NT EY K+ K + V E+ Sbjct: 150 VANEIDSYFIHVDAASIMSKWLGEAEKNVAKIFNTAREYSKKDNKPAIIFVDEI 203 >4K7C-A nrPDB Length = 446 Score = 27.7 bits (60), Expect = 7.1, Method: Composition-based stats. Identities = 36/130 (27%), Positives = 53/130 (40%), Gaps = 19/130 (14%) Query: 158 DFSEKSSYFQSQVDKI-------RKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPE 210 D EKS+YF V K RK A+ A G + ++ + G+V ++PE Sbjct: 103 DKFEKSNYFYENVLKTADQPLDSRKVAFLLATPQQDGGQWDMLSALIEKYGIVPKSVMPE 162 Query: 211 L-----KNKLKSVQNF-----FTTLSNTV--KQANKDIDAAKLKLTTEIAAIGEIKTETE 258 N+L + N TL V K ++ DI+AAK K+ E I Sbjct: 163 TYSSSKSNELNGLLNLKLRKDAVTLRKLVADKASDADIEAAKQKMLAEDYRILAYTLGNP 222 Query: 259 TTRFYVDYDD 268 T+F +Y D Sbjct: 223 PTKFDFEYRD 232 >3PYM-A nrPDB Length = 332 Score = 27.3 bits (59), Expect = 9.6, Method: Composition-based stats. Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 4/68 (5%) Query: 191 GLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSNTV----KQANKDIDAAKLKLTTE 246 G II S A GK++PEL+ KL + T+ +V + NK+ ++K + Sbjct: 202 GNIIPSSTGAAKAVGKVLPELQGKLTGMAFRVPTVDVSVVDLTVKLNKETTYDEIKKVVK 261 Query: 247 IAAIGEIK 254 AA G++K Sbjct: 262 AAAEGKLK 269 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.139 0.392 Lambda K H 0.267 0.0427 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,448,296 Number of Sequences: 36641 Number of extensions: 453760 Number of successful extensions: 1293 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 35 Number of HSP's that attempted gapping in prelim test: 1288 Number of HSP's gapped (non-prelim): 40 length of query: 303 length of database: 10,336,785 effective HSP length: 97 effective length of query: 206 effective length of database: 6,782,608 effective search space: 1397217248 effective search space used: 1397217248 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 59 (27.3 bits)