BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P77214 (110 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2VB3-X nrPDB 183 1e-47 4XI7-A nrPDB 29 0.44 5ES6-A nrPDB 29 0.52 5JNF-C nrPDB 29 0.53 5ES8-A nrPDB 29 0.60 3ODH-A nrPDB 28 0.67 5IXC-A nrPDB 27 1.3 3HRZ-A nrPDB 27 2.5 1F6F-A nrPDB 26 4.1 3MJO-A nrPDB 25 8.5 3DHZ-A nrPDB 25 8.8 5SWU-A nrPDB 25 9.6 >2VB3-X nrPDB Length = 88 Score = 183 bits (465), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 88/88 (100%), Positives = 88/88 (100%) Query: 23 NEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMS 82 NEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMS Sbjct: 1 NEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMS 60 Query: 83 EIKTGDKVAFNFVQQGNLSLLQDIKVSQ 110 EIKTGDKVAFNFVQQGNLSLLQDIKVSQ Sbjct: 61 EIKTGDKVAFNFVQQGNLSLLQDIKVSQ 88 >4XI7-A nrPDB Length = 380 Score = 29.3 bits (64), Expect = 0.44, Method: Composition-based stats. Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 12/72 (16%) Query: 21 QANEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQ-- 78 Q+ +H H ++ + ++ TG V GID + HD + V +P R+T P Sbjct: 256 QSLQHGHGGWTDGMFETLTTTGTVCGIDED-------HDIV--VQYPSGN-RWTFNPAVL 305 Query: 79 TKMSEIKTGDKV 90 TK S+ + GD V Sbjct: 306 TKASQFQVGDLV 317 >5ES6-A nrPDB Length = 685 Score = 28.9 bits (63), Expect = 0.52, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 64 VNWPEMTM-RFTITPQTKMSEIKTGDKVAFNFVQQGNLSLL 103 +N PE+T +F + PQT +TGD+ F+ GN+ L Sbjct: 543 LNRPELTAEKFIVHPQTGERMYRTGDRA--RFLPDGNIEFL 581 >5JNF-C nrPDB Length = 587 Score = 28.9 bits (63), Expect = 0.53, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 64 VNWPEMTM-RFTITPQTKMSEIKTGDKVAFNFVQQGNLSLL 103 +N PE+T +F + PQT +TGD+ F+ GN+ L Sbjct: 545 LNRPELTAEKFIVHPQTGERMYRTGDRA--RFLPDGNIEFL 583 >5ES8-A nrPDB Length = 776 Score = 28.9 bits (63), Expect = 0.60, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 64 VNWPEMTM-RFTITPQTKMSEIKTGDKVAFNFVQQGNLSLL 103 +N PE+T +F + PQT +TGD+ F+ GN+ L Sbjct: 543 LNRPELTAEKFIVHPQTGERMYRTGDRA--RFLPDGNIEFL 581 >3ODH-A nrPDB Length = 194 Score = 28.5 bits (62), Expect = 0.67, Method: Compositional matrix adjust. Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 3/73 (4%) Query: 36 QVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFV 95 +V+ G V GI + +I D I V+WPE F I P K + +K + Sbjct: 7 EVLINNGSVPGIPMILNEI---QDAIKTVSWPEGNNSFVINPVRKGNGVKPIKNSCMRHL 63 Query: 96 QQGNLSLLQDIKV 108 Q +L +++ Sbjct: 64 HQKGWALEHPVRI 76 >5IXC-A nrPDB Length = 814 Score = 27.3 bits (59), Expect = 1.3, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 5/53 (9%) Query: 28 ETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTK 80 + +SEA P VI G+ ++K +T P+ W E RF I Q K Sbjct: 42 DLLSEADPYVILQLSTAPGMKFKTKTLTDTSHPV----WNE-AFRFLIQSQVK 89 >3HRZ-A nrPDB Length = 627 Score = 26.6 bits (57), Expect = 2.5, Method: Compositional matrix adjust. Identities = 12/26 (46%), Positives = 17/26 (65%) Query: 82 SEIKTGDKVAFNFVQQGNLSLLQDIK 107 +EIK GD + NF +GN + L+ IK Sbjct: 430 TEIKPGDNLPVNFNVKGNANSLKQIK 455 >1F6F-A nrPDB Length = 199 Score = 25.8 bits (55), Expect = 4.1, Method: Compositional matrix adjust. Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 13/51 (25%) Query: 45 KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFV 95 +GI+ ESK I H I TP +K I T DK+ F V Sbjct: 49 QGINSESKVINCHTSSIT-------------TPNSKAEAINTEDKILFKLV 86 >3MJO-A nrPDB Length = 296 Score = 25.0 bits (53), Expect = 8.5, Method: Compositional matrix adjust. Identities = 14/54 (25%), Positives = 25/54 (46%), Gaps = 9/54 (16%) Query: 54 ITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIK 107 I H DP+ A+NW + P K +++ D++ NF + + DI+ Sbjct: 8 IANHTDPVKAINW-------NVIPDEK--DLEVWDRLTGNFWLPEKIPVSNDIQ 52 >3DHZ-A nrPDB Length = 329 Score = 24.6 bits (52), Expect = 8.8, Method: Compositional matrix adjust. Identities = 14/54 (25%), Positives = 25/54 (46%), Gaps = 9/54 (16%) Query: 54 ITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIK 107 I H DP+ A+NW + P K +++ D++ NF + + DI+ Sbjct: 9 IANHTDPVKAINW-------NVIPDEK--DLEVWDRLTGNFWLPEKIPVSNDIQ 53 >5SWU-A nrPDB Length = 263 Score = 24.6 bits (52), Expect = 9.6, Method: Compositional matrix adjust. Identities = 9/20 (45%), Positives = 12/20 (60%) Query: 51 SKKITIHHDPIAAVNWPEMT 70 SK I HHDP ++W M+ Sbjct: 142 SKIIASHHDPNGELSWANMS 161 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2VB3-X nrPDB 175 4e-45 Sequences not found previously or not previously below threshold: 3ODH-A nrPDB 30 0.29 4XI7-A nrPDB 29 0.43 5JNF-C nrPDB 29 0.45 5ES8-A nrPDB 29 0.45 5ES6-A nrPDB 29 0.45 5IXC-A nrPDB 28 1.1 1F6F-A nrPDB 27 1.6 3HRZ-A nrPDB 27 1.9 4RLT-A nrPDB 26 3.5 5SWU-A nrPDB 25 6.0 3MJO-A nrPDB 25 7.9 3DHZ-A nrPDB 25 7.9 2GPR-A nrPDB 25 9.4 CONVERGED! >2VB3-X nrPDB Length = 88 Score = 175 bits (443), Expect = 4e-45, Method: Composition-based stats. Identities = 88/88 (100%), Positives = 88/88 (100%) Query: 23 NEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMS 82 NEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMS Sbjct: 1 NEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMS 60 Query: 83 EIKTGDKVAFNFVQQGNLSLLQDIKVSQ 110 EIKTGDKVAFNFVQQGNLSLLQDIKVSQ Sbjct: 61 EIKTGDKVAFNFVQQGNLSLLQDIKVSQ 88 >3ODH-A nrPDB Length = 194 Score = 29.7 bits (65), Expect = 0.29, Method: Composition-based stats. Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 3/73 (4%) Query: 36 QVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFV 95 +V+ G V GI + +I D I V+WPE F I P K + +K + Sbjct: 7 EVLINNGSVPGIPMILNEI---QDAIKTVSWPEGNNSFVINPVRKGNGVKPIKNSCMRHL 63 Query: 96 QQGNLSLLQDIKV 108 Q +L +++ Sbjct: 64 HQKGWALEHPVRI 76 >4XI7-A nrPDB Length = 380 Score = 29.3 bits (64), Expect = 0.43, Method: Composition-based stats. Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 12/72 (16%) Query: 21 QANEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQ-- 78 Q+ +H H ++ + ++ TG V GID + HD + V +P R+T P Sbjct: 256 QSLQHGHGGWTDGMFETLTTTGTVCGIDED-------HDIV--VQYPSGN-RWTFNPAVL 305 Query: 79 TKMSEIKTGDKV 90 TK S+ + GD V Sbjct: 306 TKASQFQVGDLV 317 >5JNF-C nrPDB Length = 587 Score = 28.9 bits (63), Expect = 0.45, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 64 VNWPEMTM-RFTITPQTKMSEIKTGDKVAFNFVQQGNLSLL 103 +N PE+T +F + PQT +TGD+ F+ GN+ L Sbjct: 545 LNRPELTAEKFIVHPQTGERMYRTGDRA--RFLPDGNIEFL 583 >5ES8-A nrPDB Length = 776 Score = 28.9 bits (63), Expect = 0.45, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 64 VNWPEMTM-RFTITPQTKMSEIKTGDKVAFNFVQQGNLSLL 103 +N PE+T +F + PQT +TGD+ F+ GN+ L Sbjct: 543 LNRPELTAEKFIVHPQTGERMYRTGDRA--RFLPDGNIEFL 581 >5ES6-A nrPDB Length = 685 Score = 28.9 bits (63), Expect = 0.45, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 64 VNWPEMTM-RFTITPQTKMSEIKTGDKVAFNFVQQGNLSLL 103 +N PE+T +F + PQT +TGD+ F+ GN+ L Sbjct: 543 LNRPELTAEKFIVHPQTGERMYRTGDRA--RFLPDGNIEFL 581 >5IXC-A nrPDB Length = 814 Score = 27.8 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 5/53 (9%) Query: 28 ETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTK 80 + +SEA P VI G+ ++K +T P+ W E RF I Q K Sbjct: 42 DLLSEADPYVILQLSTAPGMKFKTKTLTDTSHPV----WNE-AFRFLIQSQVK 89 >1F6F-A nrPDB Length = 199 Score = 27.4 bits (59), Expect = 1.6, Method: Composition-based stats. Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 13/51 (25%) Query: 45 KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFV 95 +GI+ ESK I H I TP +K I T DK+ F V Sbjct: 49 QGINSESKVINCHTSSIT-------------TPNSKAEAINTEDKILFKLV 86 >3HRZ-A nrPDB Length = 627 Score = 27.0 bits (58), Expect = 1.9, Method: Composition-based stats. Identities = 12/26 (46%), Positives = 17/26 (65%) Query: 82 SEIKTGDKVAFNFVQQGNLSLLQDIK 107 +EIK GD + NF +GN + L+ IK Sbjct: 430 TEIKPGDNLPVNFNVKGNANSLKQIK 455 >4RLT-A nrPDB Length = 158 Score = 26.2 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 11/39 (28%), Positives = 20/39 (51%) Query: 7 VAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVK 45 +A + V G+ AQA H ++ A+ Q++ V+K Sbjct: 54 LAPLTFICVFGYKAQAAFFKHANIATAEAQIVQVDQVLK 92 >5SWU-A nrPDB Length = 263 Score = 25.5 bits (54), Expect = 6.0, Method: Composition-based stats. Identities = 9/20 (45%), Positives = 12/20 (60%) Query: 51 SKKITIHHDPIAAVNWPEMT 70 SK I HHDP ++W M+ Sbjct: 142 SKIIASHHDPNGELSWANMS 161 >3MJO-A nrPDB Length = 296 Score = 25.1 bits (53), Expect = 7.9, Method: Composition-based stats. Identities = 14/54 (25%), Positives = 25/54 (46%), Gaps = 9/54 (16%) Query: 54 ITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIK 107 I H DP+ A+NW + P K +++ D++ NF + + DI+ Sbjct: 8 IANHTDPVKAINW-------NVIPDEK--DLEVWDRLTGNFWLPEKIPVSNDIQ 52 >3DHZ-A nrPDB Length = 329 Score = 25.1 bits (53), Expect = 7.9, Method: Composition-based stats. Identities = 14/54 (25%), Positives = 25/54 (46%), Gaps = 9/54 (16%) Query: 54 ITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIK 107 I H DP+ A+NW + P K +++ D++ NF + + DI+ Sbjct: 9 IANHTDPVKAINW-------NVIPDEK--DLEVWDRLTGNFWLPEKIPVSNDIQ 53 >2GPR-A nrPDB Length = 154 Score = 24.7 bits (52), Expect = 9.4, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 7/60 (11%) Query: 34 QPQVISATGVVKGIDLES--KKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVA 91 Q Q ++A + +DL+S KK+ PI N T+ KM E+K GD VA Sbjct: 97 QDQEVNAGDKLVTVDLKSVAKKVPSIKSPIIFTNNGGKTLEIV-----KMGEVKQGDVVA 151 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.129 0.361 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,388,673 Number of Sequences: 36641 Number of extensions: 147689 Number of successful extensions: 512 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 493 Number of HSP's gapped (non-prelim): 40 length of query: 110 length of database: 10,336,785 effective HSP length: 76 effective length of query: 34 effective length of database: 7,552,069 effective search space: 256770346 effective search space used: 256770346 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)