BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P69829
(163 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
1A6J-A nrPDB 332 3e-92
2A0J-A nrPDB 104 2e-23
3URR-A nrPDB 94 3e-20
3BJV-A nrPDB 34 0.034
2D4W-A nrPDB 30 0.39
5YQ8-A nrPDB 27 2.7
2ECF-A nrPDB 27 4.0
1GJJ-A nrPDB 27 5.7
>1A6J-A nrPDB
Length = 163
Score = 332 bits (852), Expect = 3e-92, Method: Compositional matrix adjust.
Identities = 163/163 (100%), Positives = 163/163 (100%)
Query: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60
MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM
Sbjct: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60
Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120
GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH
Sbjct: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120
Query: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163
LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA
Sbjct: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163
>2A0J-A nrPDB
Length = 149
Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 55/129 (42%), Positives = 76/129 (58%), Gaps = 1/129 (0%)
Query: 27 SKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGV 86
SKKR E L ++ SL VFE + REK+GSTG+G G+AIPHG+ +A G
Sbjct: 21 SKKRLFEEAGLLLERESSLSHADVFECLFAREKLGSTGLGQGVAIPHGR-HAGVKQATGA 79
Query: 87 FVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSD 146
F++ P+ FDA D +PV L+F LLVP + T HL LS +A + + K+I L S
Sbjct: 80 FIRTREPVGFDAPDGKPVSLIFILLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSA 139
Query: 147 EELYQIITD 155
EE+ I+T+
Sbjct: 140 EEVRAILTE 148
>3URR-A nrPDB
Length = 153
Score = 93.6 bits (231), Expect = 3e-20, Method: Compositional matrix adjust.
Identities = 53/149 (35%), Positives = 82/149 (55%), Gaps = 1/149 (0%)
Query: 9 QLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNG 68
+L+ +L E + SKKR E + Q + V + + RE++GSTG+G G
Sbjct: 5 RLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEG 64
Query: 69 IAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVA 128
+AIPHG+++ + FV+L PI F+A D QPV LL LLVP T+ HL LS +A
Sbjct: 65 VAIPHGRIK-GLKHPLAAFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIA 123
Query: 129 KRLADKTICRRLRAAQSDEELYQIITDTE 157
+ L+D+ RL +EL++++T +
Sbjct: 124 QLLSDRDTRERLHTEPDRDELHRLLTQWQ 152
>3BJV-A nrPDB
Length = 161
Score = 33.9 bits (76), Expect = 0.034, Method: Compositional matrix adjust.
Identities = 24/93 (25%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Query: 68 GIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLV 127
G+A+PH + E R V L P+ F D + V +L AL + + T + ++
Sbjct: 63 GMAMPHARPEAGVQRDAFSLVTLTEPVTF--TDGKEVQVLLALAATSSKIHTSVAIPQII 120
Query: 128 AKRLADKTICRRLRAAQSDEELYQIITDTEGTP 160
A D +I RL ++ EE+ ++ +++ +P
Sbjct: 121 ALFELDHSI-ERLVNCKTPEEVLAMVEESKSSP 152
>2D4W-A nrPDB
Length = 504
Score = 30.4 bits (67), Expect = 0.39, Method: Composition-based stats.
Identities = 13/37 (35%), Positives = 21/37 (56%)
Query: 68 GIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPV 104
G+A+ G L + + AVG+ Q ET + +D +PV
Sbjct: 59 GLALTRGNLTHEDIAAVGITNQRETAVVWDKTTGKPV 95
>5YQ8-A nrPDB
Length = 130
Score = 27.3 bits (59), Expect = 2.7, Method: Compositional matrix adjust.
Identities = 19/72 (26%), Positives = 32/72 (44%), Gaps = 8/72 (11%)
Query: 55 LTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPA 114
L +M +G G G++ + G+++ P AF I++Q +DL+ L
Sbjct: 43 LVDVRMRGVAVGVGRQEICGRIHMTPVNLAGMYI----PFAFYVIEDQAMDLIIGL---- 94
Query: 115 DQTKTHLHTLSL 126
DQ K H + L
Sbjct: 95 DQLKRHQMMIDL 106
>2ECF-A nrPDB
Length = 741
Score = 26.9 bits (58), Expect = 4.0, Method: Composition-based stats.
Identities = 22/58 (37%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGV-FVQLETPIAFDAIDNQPVDLLFALLVPADQT 117
GST IGNGIA +E+ R G + ++ IA+ ID PV + V AD+T
Sbjct: 191 GSTTIGNGIA--EFVADEEMDRHTGYWWAPDDSAIAYARIDESPVPVQKRYEVYADRT 246
>1GJJ-A nrPDB
Length = 168
Score = 26.6 bits (57), Expect = 5.7, Method: Compositional matrix adjust.
Identities = 12/38 (31%), Positives = 23/38 (60%), Gaps = 6/38 (15%)
Query: 53 AILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQL 90
++LT++K+ S + N + +P G+ +D V+VQL
Sbjct: 9 SVLTKDKLKSELVANNVTLPAGEQRKD------VYVQL 40
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
1A6J-A nrPDB 251 1e-67
3URR-A nrPDB 224 1e-59
2A0J-A nrPDB 203 4e-53
Sequences not found previously or not previously below threshold:
3BJV-A nrPDB 48 2e-06
2OQT-A nrPDB 43 5e-05
4YZF-A nrPDB 35 0.017
1HYN-P nrPDB 33 0.057
4KY9-A nrPDB 33 0.060
2OQ3-A nrPDB 32 0.11
3C1J-A nrPDB 29 0.91
5H9F-B nrPDB 29 1.1
4BPA-A nrPDB 29 1.3
4HST-B nrPDB 29 1.4
3OQC-A nrPDB 28 1.7
5DWV-A nrPDB 27 4.3
5Y44-A nrPDB 26 6.9
>1A6J-A nrPDB
Length = 163
Score = 251 bits (640), Expect = 1e-67, Method: Composition-based stats.
Identities = 163/163 (100%), Positives = 163/163 (100%)
Query: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60
MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM
Sbjct: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60
Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120
GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH
Sbjct: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120
Query: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163
LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA
Sbjct: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163
>3URR-A nrPDB
Length = 153
Score = 224 bits (572), Expect = 1e-59, Method: Composition-based stats.
Identities = 53/149 (35%), Positives = 82/149 (55%), Gaps = 1/149 (0%)
Query: 9 QLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNG 68
+L+ +L E + SKKR E + Q + V + + RE++GSTG+G G
Sbjct: 5 RLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEG 64
Query: 69 IAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVA 128
+AIPHG+++ + FV+L PI F+A D QPV LL LLVP T+ HL LS +A
Sbjct: 65 VAIPHGRIK-GLKHPLAAFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIA 123
Query: 129 KRLADKTICRRLRAAQSDEELYQIITDTE 157
+ L+D+ RL +EL++++T +
Sbjct: 124 QLLSDRDTRERLHTEPDRDELHRLLTQWQ 152
>2A0J-A nrPDB
Length = 149
Score = 203 bits (515), Expect = 4e-53, Method: Composition-based stats.
Identities = 56/146 (38%), Positives = 80/146 (54%), Gaps = 1/146 (0%)
Query: 10 LSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGI 69
+ +L + SKKR E L ++ SL VFE + REK+GSTG+G G+
Sbjct: 4 IGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADVFECLFAREKLGSTGLGQGV 63
Query: 70 AIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAK 129
AIPHG+ +A G F++ P+ FDA D +PV L+F LLVP + T HL LS +A
Sbjct: 64 AIPHGR-HAGVKQATGAFIRTREPVGFDAPDGKPVSLIFILLVPENATGEHLEVLSKLAG 122
Query: 130 RLADKTICRRLRAAQSDEELYQIITD 155
+ + K+I L S EE+ I+T+
Sbjct: 123 KFSQKSIRESLMTVSSAEEVRAILTE 148
>3BJV-A nrPDB
Length = 161
Score = 47.8 bits (112), Expect = 2e-06, Method: Composition-based stats.
Identities = 24/93 (25%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Query: 68 GIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLV 127
G+A+PH + E R V L P+ F D + V +L AL + + T + ++
Sbjct: 63 GMAMPHARPEAGVQRDAFSLVTLTEPVTF--TDGKEVQVLLALAATSSKIHTSVAIPQII 120
Query: 128 AKRLADKTICRRLRAAQSDEELYQIITDTEGTP 160
A D +I RL ++ EE+ ++ +++ +P
Sbjct: 121 ALFELDHSI-ERLVNCKTPEEVLAMVEESKSSP 152
>2OQT-A nrPDB
Length = 162
Score = 43.1 bits (100), Expect = 5e-05, Method: Composition-based stats.
Identities = 30/150 (20%), Positives = 62/150 (41%), Gaps = 4/150 (2%)
Query: 12 SVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTG-IGNGIA 70
++++ R + + + A+ + + ++ I + EK G + G+A
Sbjct: 7 ALIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVLMPGMA 66
Query: 71 IPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAKR 130
+PH + R + L P+ F D + V +L L T + +VA
Sbjct: 67 MPHAEAGLGVNRNAFALITLTKPVTFS--DGKEVSVLLTLAATDPSIHTTVAIPQIVALF 124
Query: 131 LADKTICRRLRAAQSDEELYQIITDTEGTP 160
D I RL A QS +E+ +++ +++ +P
Sbjct: 125 ELDNAI-ERLVACQSPKEVLEMVEESKDSP 153
>4YZF-A nrPDB
Length = 911
Score = 34.7 bits (78), Expect = 0.017, Method: Composition-based stats.
Identities = 17/71 (23%), Positives = 29/71 (40%), Gaps = 1/71 (1%)
Query: 80 TLRAVGVFVQLETPIAFDAID-NQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICR 138
+ V FV+L+ +A++ P+ LF LL P + A ++++
Sbjct: 237 LEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRI 296
Query: 139 RLRAAQSDEEL 149
AQS EL
Sbjct: 297 DAYMAQSRGEL 307
>1HYN-P nrPDB
Length = 379
Score = 33.1 bits (74), Expect = 0.057, Method: Composition-based stats.
Identities = 17/71 (23%), Positives = 29/71 (40%), Gaps = 1/71 (1%)
Query: 80 TLRAVGVFVQLETPIAFDAID-NQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICR 138
+ V FV+L+ +A++ P+ LF LL P + A ++++
Sbjct: 237 LEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRI 296
Query: 139 RLRAAQSDEEL 149
AQS EL
Sbjct: 297 DAYMAQSRGEL 307
>4KY9-A nrPDB
Length = 306
Score = 33.1 bits (74), Expect = 0.060, Method: Composition-based stats.
Identities = 17/71 (23%), Positives = 29/71 (40%), Gaps = 1/71 (1%)
Query: 80 TLRAVGVFVQLETPIAFDAID-NQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICR 138
+ V FV+L+ +A++ P+ LF LL P + A ++++
Sbjct: 187 LEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRI 246
Query: 139 RLRAAQSDEEL 149
AQS EL
Sbjct: 247 DAYMAQSRGEL 257
>2OQ3-A nrPDB
Length = 150
Score = 32.0 bits (71), Expect = 0.11, Method: Composition-based stats.
Identities = 24/90 (26%), Positives = 45/90 (50%), Gaps = 3/90 (3%)
Query: 65 IGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTL 124
+ G+A+PH + E L+ LE + F D +P+ LL L A +H+ +
Sbjct: 62 LAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGND-EPIKLLIGL--SAADADSHIGAI 118
Query: 125 SLVAKRLADKTICRRLRAAQSDEELYQIIT 154
+++ L ++ I +L A S+++L II+
Sbjct: 119 QALSELLCEEEILEQLLTASSEKQLADIIS 148
>3C1J-A nrPDB
Length = 424
Score = 28.9 bits (63), Expect = 0.91, Method: Composition-based stats.
Identities = 18/76 (23%), Positives = 31/76 (40%), Gaps = 4/76 (5%)
Query: 50 VFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFA 109
+ I +G G G+ + H L + L ++ + + +AF I + DL
Sbjct: 327 IMTGIFAASSLGGVGFAEGVTMGHQLLVQ--LESIAITIVWSGVVAF--IGYKLADLTVG 382
Query: 110 LLVPADQTKTHLHTLS 125
L VP +Q + L S
Sbjct: 383 LRVPEEQEREGLDVNS 398
>5H9F-B nrPDB
Length = 165
Score = 28.9 bits (63), Expect = 1.1, Method: Composition-based stats.
Identities = 19/100 (19%), Positives = 38/100 (38%), Gaps = 15/100 (15%)
Query: 77 EEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLV-------------PADQTKTHLHT 123
E D LR + F +L P ++ +Q L + T L
Sbjct: 35 EPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGR 94
Query: 124 LSLVAKRLADKTICRRLRAAQSDE--ELYQIITDTEGTPD 161
+ R+ ++ I + +RA ++ + +L +++T E D
Sbjct: 95 ALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLD 134
>4BPA-A nrPDB
Length = 259
Score = 28.5 bits (62), Expect = 1.3, Method: Composition-based stats.
Identities = 24/80 (30%), Positives = 33/80 (41%), Gaps = 11/80 (13%)
Query: 33 EIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGIAIPH-GKLEEDTLRAVGVFVQLE 91
E+ LA LP F+ L R +G +P G+LE+DT +G F
Sbjct: 186 ELARRLAELNGQLPDVRWFQQQLAR---------HGYLVPQTGELEKDTRDVIGAFQMKY 236
Query: 92 TPIAFDA-IDNQPVDLLFAL 110
P FD D + LL A+
Sbjct: 237 RPARFDGEPDLETAALLLAV 256
>4HST-B nrPDB
Length = 543
Score = 28.5 bits (62), Expect = 1.4, Method: Composition-based stats.
Identities = 13/50 (26%), Positives = 18/50 (36%)
Query: 114 ADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163
D H TLS L + R + E L Q++ +G D A
Sbjct: 280 DDAAAIHADTLSPHVGLLRRRLEALGARDDSAAEGLRQMLVAWDGRMDAA 329
>3OQC-A nrPDB
Length = 481
Score = 28.1 bits (61), Expect = 1.7, Method: Composition-based stats.
Identities = 15/68 (22%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
Query: 85 GVFVQLETPIAFDAIDNQP-VDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAA 143
G + + P+ F + V +L+ +P DQ + + L + D+ +R+ A
Sbjct: 202 GTSIVVPEPLHFQLPGKKNLVTVLYPSGIPDDQLQAYRKELHDLFNLPHDRPYFKRINAY 261
Query: 144 QSDEELYQ 151
+ELY+
Sbjct: 262 HFPDELYK 269
>5DWV-A nrPDB
Length = 573
Score = 27.0 bits (58), Expect = 4.3, Method: Composition-based stats.
Identities = 12/34 (35%), Positives = 15/34 (44%), Gaps = 7/34 (20%)
Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPI 94
GSTG+ G+A+PH A F PI
Sbjct: 223 GSTGLPKGVALPH-------RTACVRFSHARDPI 249
>5Y44-A nrPDB
Length = 227
Score = 26.2 bits (56), Expect = 6.9, Method: Composition-based stats.
Identities = 14/45 (31%), Positives = 22/45 (48%)
Query: 111 LVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITD 155
L P QT H S +R+ + + L+ A S EE + I+T+
Sbjct: 2 LTPDQQTLLHFIMDSYNKQRMPQEITNKILKEAFSAEENFLILTE 46
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.311 0.133 0.337
Lambda K H
0.267 0.0409 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,544,078
Number of Sequences: 36641
Number of extensions: 193038
Number of successful extensions: 546
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 525
Number of HSP's gapped (non-prelim): 32
length of query: 163
length of database: 10,336,785
effective HSP length: 90
effective length of query: 73
effective length of database: 7,039,095
effective search space: 513853935
effective search space used: 513853935
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 55 (25.8 bits)