BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P69829 (163 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1A6J-A nrPDB 332 3e-92 2A0J-A nrPDB 104 2e-23 3URR-A nrPDB 94 3e-20 3BJV-A nrPDB 34 0.034 2D4W-A nrPDB 30 0.39 5YQ8-A nrPDB 27 2.7 2ECF-A nrPDB 27 4.0 1GJJ-A nrPDB 27 5.7 >1A6J-A nrPDB Length = 163 Score = 332 bits (852), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 163/163 (100%), Positives = 163/163 (100%) Query: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM Sbjct: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60 Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH Sbjct: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120 Query: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA Sbjct: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163 >2A0J-A nrPDB Length = 149 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 55/129 (42%), Positives = 76/129 (58%), Gaps = 1/129 (0%) Query: 27 SKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGV 86 SKKR E L ++ SL VFE + REK+GSTG+G G+AIPHG+ +A G Sbjct: 21 SKKRLFEEAGLLLERESSLSHADVFECLFAREKLGSTGLGQGVAIPHGR-HAGVKQATGA 79 Query: 87 FVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSD 146 F++ P+ FDA D +PV L+F LLVP + T HL LS +A + + K+I L S Sbjct: 80 FIRTREPVGFDAPDGKPVSLIFILLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSA 139 Query: 147 EELYQIITD 155 EE+ I+T+ Sbjct: 140 EEVRAILTE 148 >3URR-A nrPDB Length = 153 Score = 93.6 bits (231), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 53/149 (35%), Positives = 82/149 (55%), Gaps = 1/149 (0%) Query: 9 QLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNG 68 +L+ +L E + SKKR E + Q + V + + RE++GSTG+G G Sbjct: 5 RLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEG 64 Query: 69 IAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVA 128 +AIPHG+++ + FV+L PI F+A D QPV LL LLVP T+ HL LS +A Sbjct: 65 VAIPHGRIK-GLKHPLAAFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIA 123 Query: 129 KRLADKTICRRLRAAQSDEELYQIITDTE 157 + L+D+ RL +EL++++T + Sbjct: 124 QLLSDRDTRERLHTEPDRDELHRLLTQWQ 152 >3BJV-A nrPDB Length = 161 Score = 33.9 bits (76), Expect = 0.034, Method: Compositional matrix adjust. Identities = 24/93 (25%), Positives = 45/93 (48%), Gaps = 3/93 (3%) Query: 68 GIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLV 127 G+A+PH + E R V L P+ F D + V +L AL + + T + ++ Sbjct: 63 GMAMPHARPEAGVQRDAFSLVTLTEPVTF--TDGKEVQVLLALAATSSKIHTSVAIPQII 120 Query: 128 AKRLADKTICRRLRAAQSDEELYQIITDTEGTP 160 A D +I RL ++ EE+ ++ +++ +P Sbjct: 121 ALFELDHSI-ERLVNCKTPEEVLAMVEESKSSP 152 >2D4W-A nrPDB Length = 504 Score = 30.4 bits (67), Expect = 0.39, Method: Composition-based stats. Identities = 13/37 (35%), Positives = 21/37 (56%) Query: 68 GIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPV 104 G+A+ G L + + AVG+ Q ET + +D +PV Sbjct: 59 GLALTRGNLTHEDIAAVGITNQRETAVVWDKTTGKPV 95 >5YQ8-A nrPDB Length = 130 Score = 27.3 bits (59), Expect = 2.7, Method: Compositional matrix adjust. Identities = 19/72 (26%), Positives = 32/72 (44%), Gaps = 8/72 (11%) Query: 55 LTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPA 114 L +M +G G G++ + G+++ P AF I++Q +DL+ L Sbjct: 43 LVDVRMRGVAVGVGRQEICGRIHMTPVNLAGMYI----PFAFYVIEDQAMDLIIGL---- 94 Query: 115 DQTKTHLHTLSL 126 DQ K H + L Sbjct: 95 DQLKRHQMMIDL 106 >2ECF-A nrPDB Length = 741 Score = 26.9 bits (58), Expect = 4.0, Method: Composition-based stats. Identities = 22/58 (37%), Positives = 30/58 (51%), Gaps = 3/58 (5%) Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGV-FVQLETPIAFDAIDNQPVDLLFALLVPADQT 117 GST IGNGIA +E+ R G + ++ IA+ ID PV + V AD+T Sbjct: 191 GSTTIGNGIA--EFVADEEMDRHTGYWWAPDDSAIAYARIDESPVPVQKRYEVYADRT 246 >1GJJ-A nrPDB Length = 168 Score = 26.6 bits (57), Expect = 5.7, Method: Compositional matrix adjust. Identities = 12/38 (31%), Positives = 23/38 (60%), Gaps = 6/38 (15%) Query: 53 AILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQL 90 ++LT++K+ S + N + +P G+ +D V+VQL Sbjct: 9 SVLTKDKLKSELVANNVTLPAGEQRKD------VYVQL 40 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1A6J-A nrPDB 251 1e-67 3URR-A nrPDB 224 1e-59 2A0J-A nrPDB 203 4e-53 Sequences not found previously or not previously below threshold: 3BJV-A nrPDB 48 2e-06 2OQT-A nrPDB 43 5e-05 4YZF-A nrPDB 35 0.017 1HYN-P nrPDB 33 0.057 4KY9-A nrPDB 33 0.060 2OQ3-A nrPDB 32 0.11 3C1J-A nrPDB 29 0.91 5H9F-B nrPDB 29 1.1 4BPA-A nrPDB 29 1.3 4HST-B nrPDB 29 1.4 3OQC-A nrPDB 28 1.7 5DWV-A nrPDB 27 4.3 5Y44-A nrPDB 26 6.9 >1A6J-A nrPDB Length = 163 Score = 251 bits (640), Expect = 1e-67, Method: Composition-based stats. Identities = 163/163 (100%), Positives = 163/163 (100%) Query: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM Sbjct: 1 MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM 60 Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH Sbjct: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH 120 Query: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA Sbjct: 121 LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163 >3URR-A nrPDB Length = 153 Score = 224 bits (572), Expect = 1e-59, Method: Composition-based stats. Identities = 53/149 (35%), Positives = 82/149 (55%), Gaps = 1/149 (0%) Query: 9 QLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNG 68 +L+ +L E + SKKR E + Q + V + + RE++GSTG+G G Sbjct: 5 RLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEG 64 Query: 69 IAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVA 128 +AIPHG+++ + FV+L PI F+A D QPV LL LLVP T+ HL LS +A Sbjct: 65 VAIPHGRIK-GLKHPLAAFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIA 123 Query: 129 KRLADKTICRRLRAAQSDEELYQIITDTE 157 + L+D+ RL +EL++++T + Sbjct: 124 QLLSDRDTRERLHTEPDRDELHRLLTQWQ 152 >2A0J-A nrPDB Length = 149 Score = 203 bits (515), Expect = 4e-53, Method: Composition-based stats. Identities = 56/146 (38%), Positives = 80/146 (54%), Gaps = 1/146 (0%) Query: 10 LSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGI 69 + +L + SKKR E L ++ SL VFE + REK+GSTG+G G+ Sbjct: 4 IGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADVFECLFAREKLGSTGLGQGV 63 Query: 70 AIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAK 129 AIPHG+ +A G F++ P+ FDA D +PV L+F LLVP + T HL LS +A Sbjct: 64 AIPHGR-HAGVKQATGAFIRTREPVGFDAPDGKPVSLIFILLVPENATGEHLEVLSKLAG 122 Query: 130 RLADKTICRRLRAAQSDEELYQIITD 155 + + K+I L S EE+ I+T+ Sbjct: 123 KFSQKSIRESLMTVSSAEEVRAILTE 148 >3BJV-A nrPDB Length = 161 Score = 47.8 bits (112), Expect = 2e-06, Method: Composition-based stats. Identities = 24/93 (25%), Positives = 45/93 (48%), Gaps = 3/93 (3%) Query: 68 GIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLV 127 G+A+PH + E R V L P+ F D + V +L AL + + T + ++ Sbjct: 63 GMAMPHARPEAGVQRDAFSLVTLTEPVTF--TDGKEVQVLLALAATSSKIHTSVAIPQII 120 Query: 128 AKRLADKTICRRLRAAQSDEELYQIITDTEGTP 160 A D +I RL ++ EE+ ++ +++ +P Sbjct: 121 ALFELDHSI-ERLVNCKTPEEVLAMVEESKSSP 152 >2OQT-A nrPDB Length = 162 Score = 43.1 bits (100), Expect = 5e-05, Method: Composition-based stats. Identities = 30/150 (20%), Positives = 62/150 (41%), Gaps = 4/150 (2%) Query: 12 SVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTG-IGNGIA 70 ++++ R + + + A+ + + ++ I + EK G + G+A Sbjct: 7 ALIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVLMPGMA 66 Query: 71 IPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAKR 130 +PH + R + L P+ F D + V +L L T + +VA Sbjct: 67 MPHAEAGLGVNRNAFALITLTKPVTFS--DGKEVSVLLTLAATDPSIHTTVAIPQIVALF 124 Query: 131 LADKTICRRLRAAQSDEELYQIITDTEGTP 160 D I RL A QS +E+ +++ +++ +P Sbjct: 125 ELDNAI-ERLVACQSPKEVLEMVEESKDSP 153 >4YZF-A nrPDB Length = 911 Score = 34.7 bits (78), Expect = 0.017, Method: Composition-based stats. Identities = 17/71 (23%), Positives = 29/71 (40%), Gaps = 1/71 (1%) Query: 80 TLRAVGVFVQLETPIAFDAID-NQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICR 138 + V FV+L+ +A++ P+ LF LL P + A ++++ Sbjct: 237 LEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRI 296 Query: 139 RLRAAQSDEEL 149 AQS EL Sbjct: 297 DAYMAQSRGEL 307 >1HYN-P nrPDB Length = 379 Score = 33.1 bits (74), Expect = 0.057, Method: Composition-based stats. Identities = 17/71 (23%), Positives = 29/71 (40%), Gaps = 1/71 (1%) Query: 80 TLRAVGVFVQLETPIAFDAID-NQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICR 138 + V FV+L+ +A++ P+ LF LL P + A ++++ Sbjct: 237 LEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRI 296 Query: 139 RLRAAQSDEEL 149 AQS EL Sbjct: 297 DAYMAQSRGEL 307 >4KY9-A nrPDB Length = 306 Score = 33.1 bits (74), Expect = 0.060, Method: Composition-based stats. Identities = 17/71 (23%), Positives = 29/71 (40%), Gaps = 1/71 (1%) Query: 80 TLRAVGVFVQLETPIAFDAID-NQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICR 138 + V FV+L+ +A++ P+ LF LL P + A ++++ Sbjct: 187 LEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRI 246 Query: 139 RLRAAQSDEEL 149 AQS EL Sbjct: 247 DAYMAQSRGEL 257 >2OQ3-A nrPDB Length = 150 Score = 32.0 bits (71), Expect = 0.11, Method: Composition-based stats. Identities = 24/90 (26%), Positives = 45/90 (50%), Gaps = 3/90 (3%) Query: 65 IGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTL 124 + G+A+PH + E L+ LE + F D +P+ LL L A +H+ + Sbjct: 62 LAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGND-EPIKLLIGL--SAADADSHIGAI 118 Query: 125 SLVAKRLADKTICRRLRAAQSDEELYQIIT 154 +++ L ++ I +L A S+++L II+ Sbjct: 119 QALSELLCEEEILEQLLTASSEKQLADIIS 148 >3C1J-A nrPDB Length = 424 Score = 28.9 bits (63), Expect = 0.91, Method: Composition-based stats. Identities = 18/76 (23%), Positives = 31/76 (40%), Gaps = 4/76 (5%) Query: 50 VFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFA 109 + I +G G G+ + H L + L ++ + + +AF I + DL Sbjct: 327 IMTGIFAASSLGGVGFAEGVTMGHQLLVQ--LESIAITIVWSGVVAF--IGYKLADLTVG 382 Query: 110 LLVPADQTKTHLHTLS 125 L VP +Q + L S Sbjct: 383 LRVPEEQEREGLDVNS 398 >5H9F-B nrPDB Length = 165 Score = 28.9 bits (63), Expect = 1.1, Method: Composition-based stats. Identities = 19/100 (19%), Positives = 38/100 (38%), Gaps = 15/100 (15%) Query: 77 EEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLV-------------PADQTKTHLHT 123 E D LR + F +L P ++ +Q L + T L Sbjct: 35 EPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGR 94 Query: 124 LSLVAKRLADKTICRRLRAAQSDE--ELYQIITDTEGTPD 161 + R+ ++ I + +RA ++ + +L +++T E D Sbjct: 95 ALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLD 134 >4BPA-A nrPDB Length = 259 Score = 28.5 bits (62), Expect = 1.3, Method: Composition-based stats. Identities = 24/80 (30%), Positives = 33/80 (41%), Gaps = 11/80 (13%) Query: 33 EIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGIAIPH-GKLEEDTLRAVGVFVQLE 91 E+ LA LP F+ L R +G +P G+LE+DT +G F Sbjct: 186 ELARRLAELNGQLPDVRWFQQQLAR---------HGYLVPQTGELEKDTRDVIGAFQMKY 236 Query: 92 TPIAFDA-IDNQPVDLLFAL 110 P FD D + LL A+ Sbjct: 237 RPARFDGEPDLETAALLLAV 256 >4HST-B nrPDB Length = 543 Score = 28.5 bits (62), Expect = 1.4, Method: Composition-based stats. Identities = 13/50 (26%), Positives = 18/50 (36%) Query: 114 ADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA 163 D H TLS L + R + E L Q++ +G D A Sbjct: 280 DDAAAIHADTLSPHVGLLRRRLEALGARDDSAAEGLRQMLVAWDGRMDAA 329 >3OQC-A nrPDB Length = 481 Score = 28.1 bits (61), Expect = 1.7, Method: Composition-based stats. Identities = 15/68 (22%), Positives = 30/68 (44%), Gaps = 1/68 (1%) Query: 85 GVFVQLETPIAFDAIDNQP-VDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAA 143 G + + P+ F + V +L+ +P DQ + + L + D+ +R+ A Sbjct: 202 GTSIVVPEPLHFQLPGKKNLVTVLYPSGIPDDQLQAYRKELHDLFNLPHDRPYFKRINAY 261 Query: 144 QSDEELYQ 151 +ELY+ Sbjct: 262 HFPDELYK 269 >5DWV-A nrPDB Length = 573 Score = 27.0 bits (58), Expect = 4.3, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 15/34 (44%), Gaps = 7/34 (20%) Query: 61 GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPI 94 GSTG+ G+A+PH A F PI Sbjct: 223 GSTGLPKGVALPH-------RTACVRFSHARDPI 249 >5Y44-A nrPDB Length = 227 Score = 26.2 bits (56), Expect = 6.9, Method: Composition-based stats. Identities = 14/45 (31%), Positives = 22/45 (48%) Query: 111 LVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITD 155 L P QT H S +R+ + + L+ A S EE + I+T+ Sbjct: 2 LTPDQQTLLHFIMDSYNKQRMPQEITNKILKEAFSAEENFLILTE 46 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.133 0.337 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,544,078 Number of Sequences: 36641 Number of extensions: 193038 Number of successful extensions: 546 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 525 Number of HSP's gapped (non-prelim): 32 length of query: 163 length of database: 10,336,785 effective HSP length: 90 effective length of query: 73 effective length of database: 7,039,095 effective search space: 513853935 effective search space used: 513853935 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 55 (25.8 bits)