BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P69300 (92 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5Y70-A nrPDB 182 4e-47 3S84-A nrPDB 29 0.60 3IBV-A nrPDB 28 0.82 1W3W-A nrPDB 27 2.9 3RBM-A nrPDB 25 7.1 1FCV-A nrPDB 25 7.1 >5Y70-A nrPDB Length = 100 Score = 182 bits (461), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 92/92 (100%), Positives = 92/92 (100%) Query: 1 MATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTD 60 MATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTD Sbjct: 1 MATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTD 60 Query: 61 KFNKKMREHSEHFKAKFAELLEQQKNAQFPGK 92 KFNKKMREHSEHFKAKFAELLEQQKNAQFPGK Sbjct: 61 KFNKKMREHSEHFKAKFAELLEQQKNAQFPGK 92 >3S84-A nrPDB Length = 273 Score = 28.9 bits (63), Expect = 0.60, Method: Compositional matrix adjust. Identities = 12/51 (23%), Positives = 28/51 (54%) Query: 40 LSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAELLEQQKNAQFP 90 L ++ + ++ E++++E+ D +R H++ KAK + +E+ K P Sbjct: 71 LRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTP 121 >3IBV-A nrPDB Length = 980 Score = 28.1 bits (61), Expect = 0.82, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 5/55 (9%) Query: 15 LDAEFAKKMEEQNKRFFADKPDEATLSPEMKEH-----YEKFEKMIQEHTDKFNK 64 D AK + Q A K D + LSPE+KE+ Y F +I+ +D +++ Sbjct: 293 FDEHVAKLINAQGVELVAIKSDPSELSPELKENCSFQLYNLFPYLIRYLSDDYDE 347 >1W3W-A nrPDB Length = 327 Score = 26.6 bits (57), Expect = 2.9, Method: Compositional matrix adjust. Identities = 17/62 (27%), Positives = 30/62 (48%) Query: 31 FADKPDEATLSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAELLEQQKNAQFP 90 F PD TL MK + +I T + N + ++ ++ FKA+F + L + ++ Sbjct: 21 FNPDPDAETLYKAMKGIGTNEQAIIDVLTKRSNTQRQQIAKSFKAQFGKDLTETLKSELS 80 Query: 91 GK 92 GK Sbjct: 81 GK 82 >3RBM-A nrPDB Length = 396 Score = 25.0 bits (53), Expect = 7.1, Method: Composition-based stats. Identities = 11/40 (27%), Positives = 23/40 (57%) Query: 19 FAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEH 58 F + M ++ + F +E +L E+KEH K+ K++ ++ Sbjct: 36 FFRNMYDKYRDAFLSHLNEYSLEEEIKEHISKYYKLLFDY 75 >1FCV-A nrPDB Length = 350 Score = 25.0 bits (53), Expect = 7.1, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 18/32 (56%) Query: 36 DEATLSPEMKEHYEKFEKMIQEHTDKFNKKMR 67 D+ + E K +EK+ ++ E T K K+MR Sbjct: 145 DDQRVEQEAKRRFEKYGQLFMEETLKAAKRMR 176 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5Y70-A nrPDB 118 5e-28 Sequences not found previously or not previously below threshold: 3IBV-A nrPDB 30 0.20 5YFP-B nrPDB 29 0.39 1W3W-A nrPDB 29 0.46 2O5R-A nrPDB 28 0.78 5LKB-A nrPDB 28 0.91 4L42-A nrPDB 28 1.1 1FCV-A nrPDB 27 1.7 3S84-A nrPDB 27 2.4 4ME9-A nrPDB 27 2.5 5LNF-A nrPDB 26 3.6 4UTP-A nrPDB 26 3.7 4D1E-A nrPDB 25 5.6 3ZK8-A nrPDB 25 6.3 5JW9-B nrPDB 25 6.8 1LHT-A nrPDB 25 8.9 2WL8-A nrPDB 25 9.4 CONVERGED! >5Y70-A nrPDB Length = 100 Score = 118 bits (296), Expect = 5e-28, Method: Composition-based stats. Identities = 92/92 (100%), Positives = 92/92 (100%) Query: 1 MATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTD 60 MATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTD Sbjct: 1 MATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTD 60 Query: 61 KFNKKMREHSEHFKAKFAELLEQQKNAQFPGK 92 KFNKKMREHSEHFKAKFAELLEQQKNAQFPGK Sbjct: 61 KFNKKMREHSEHFKAKFAELLEQQKNAQFPGK 92 >3IBV-A nrPDB Length = 980 Score = 30.1 bits (66), Expect = 0.20, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 5/55 (9%) Query: 15 LDAEFAKKMEEQNKRFFADKPDEATLSPEMKEH-----YEKFEKMIQEHTDKFNK 64 D AK + Q A K D + LSPE+KE+ Y F +I+ +D +++ Sbjct: 293 FDEHVAKLINAQGVELVAIKSDPSELSPELKENCSFQLYNLFPYLIRYLSDDYDE 347 >5YFP-B nrPDB Length = 971 Score = 29.3 bits (64), Expect = 0.39, Method: Composition-based stats. Identities = 24/82 (29%), Positives = 42/82 (51%), Gaps = 9/82 (10%) Query: 3 TTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTDKF 62 T Y + KLD++ EF +K E+N+ D P E ++ E +K +++I+ T K Sbjct: 156 TKYVKIKNKLDQIYKEFDEKTNEKNQ---CDSPKENQINVESLN--KKVDEVIRTTTFKL 210 Query: 63 NKKMREHSE--HFKA--KFAEL 80 M + + +++A KF EL Sbjct: 211 KPLMDNYQKILNYQATKKFIEL 232 >1W3W-A nrPDB Length = 327 Score = 29.0 bits (63), Expect = 0.46, Method: Composition-based stats. Identities = 17/62 (27%), Positives = 30/62 (48%) Query: 31 FADKPDEATLSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAELLEQQKNAQFP 90 F PD TL MK + +I T + N + ++ ++ FKA+F + L + ++ Sbjct: 21 FNPDPDAETLYKAMKGIGTNEQAIIDVLTKRSNTQRQQIAKSFKAQFGKDLTETLKSELS 80 Query: 91 GK 92 GK Sbjct: 81 GK 82 >2O5R-A nrPDB Length = 481 Score = 28.2 bits (61), Expect = 0.78, Method: Composition-based stats. Identities = 19/73 (26%), Positives = 35/73 (47%), Gaps = 9/73 (12%) Query: 6 EEFAAKLDRLDAEFAKKMEE--QNKRFFADKPDEATLSPEMKEHYEKFEKMIQ------- 56 EE+ K+ + E + + E + RFF + P + EMKE + + ++ +Q Sbjct: 363 EEYFKKVLEITKERVEVLSEFPEESRFFFEDPAPVEIPEEMKEVFSQLKEELQNVRWTME 422 Query: 57 EHTDKFNKKMREH 69 E T F K +++H Sbjct: 423 EITPVFKKVLKQH 435 >5LKB-A nrPDB Length = 378 Score = 28.2 bits (61), Expect = 0.91, Method: Composition-based stats. Identities = 21/95 (22%), Positives = 40/95 (42%), Gaps = 8/95 (8%) Query: 2 ATTYEEFA----AKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQ- 56 ++ ++EF K D + A+ ++++ N + D + E E +E + Sbjct: 183 SSAFDEFVDDDHKKTDLVPAQLKTQIDDFNSWVY-DSINNGVYKTGFAEKAEVYESEVNN 241 Query: 57 --EHTDKFNKKMREHSEHFKAKFAELLEQQKNAQF 89 EH DK K + + KAK+ E Q+ +F Sbjct: 242 VFEHLDKVEKILSDKYSKLKAKYGEEDRQKILGEF 276 >4L42-A nrPDB Length = 346 Score = 27.8 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 3/36 (8%) Query: 17 AEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFE 52 ++F K Q D PD++TLS E +E + FE Sbjct: 309 SQFDSKFTRQTP---VDSPDDSTLSEEEQEMFRDFE 341 >1FCV-A nrPDB Length = 350 Score = 27.0 bits (58), Expect = 1.7, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 18/32 (56%) Query: 36 DEATLSPEMKEHYEKFEKMIQEHTDKFNKKMR 67 D+ + E K +EK+ ++ E T K K+MR Sbjct: 145 DDQRVEQEAKRRFEKYGQLFMEETLKAAKRMR 176 >3S84-A nrPDB Length = 273 Score = 26.6 bits (57), Expect = 2.4, Method: Composition-based stats. Identities = 12/51 (23%), Positives = 28/51 (54%) Query: 40 LSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAELLEQQKNAQFP 90 L ++ + ++ E++++E+ D +R H++ KAK + +E+ K P Sbjct: 71 LRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTP 121 >4ME9-A nrPDB Length = 189 Score = 26.6 bits (57), Expect = 2.5, Method: Composition-based stats. Identities = 14/61 (22%), Positives = 27/61 (44%) Query: 2 ATTYEEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTDK 61 T Y F K +++ F+K M + ++ D P EA + + Y + + + + D Sbjct: 40 GTIYRYFENKEALVNSLFSKSMLQLSEMIKTDFPVEANIREQFSHTYNRLFEFARNNVDA 99 Query: 62 F 62 F Sbjct: 100 F 100 >5LNF-A nrPDB Length = 139 Score = 26.3 bits (56), Expect = 3.6, Method: Composition-based stats. Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 11/61 (18%) Query: 40 LSPEMKEHYEKFEKMIQEH-----TDKFNKKMREHS------EHFKAKFAELLEQQKNAQ 88 L P +KE EK+ + +Q H ++F K +HS E F+A+ E + A+ Sbjct: 28 LYPSLKEITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCKICEQFEAETPTDSETTQKAR 87 Query: 89 F 89 F Sbjct: 88 F 88 >4UTP-A nrPDB Length = 314 Score = 26.3 bits (56), Expect = 3.7, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 23/40 (57%) Query: 40 LSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAE 79 LS + + E +EK ++E+TDK +K +E + F AE Sbjct: 158 LSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAE 197 >4D1E-A nrPDB Length = 876 Score = 25.5 bits (54), Expect = 5.6, Method: Composition-based stats. Identities = 23/102 (22%), Positives = 43/102 (42%), Gaps = 16/102 (15%) Query: 1 MATTYEEFAAKLDRLDAEFAKKMEEQNK----------RFFADKPDEATLSPEMKEHYEK 50 + T +E+F A L D E M QN+ R + P E++ ++K Sbjct: 536 LITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDK 595 Query: 51 FEKMIQEHTDKFNKKM-REHS-EHFKAKFAELLEQQKNAQFP 90 ++++ +++ R+H+ E + +FA Q NA P Sbjct: 596 VKQLVPIRDQSLQEELARQHANERLRRQFA----AQANAIGP 633 >3ZK8-A nrPDB Length = 278 Score = 25.5 bits (54), Expect = 6.3, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 23/40 (57%) Query: 40 LSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAE 79 LS + + E +EK ++E+TDK +K +E + F AE Sbjct: 127 LSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAE 166 >5JW9-B nrPDB Length = 122 Score = 25.1 bits (53), Expect = 6.8, Method: Composition-based stats. Identities = 19/72 (26%), Positives = 33/72 (45%), Gaps = 1/72 (1%) Query: 6 EEFAAKLDRLDAEFAKKMEEQNKRFFADKPDEATLSPEMKEHYEKFEKMIQEHTDKFNKK 65 ++F A+ D A A+ ME +RF LSP KE+ E+++QE+ Sbjct: 28 DDFNAEYDEYRALHAR-METVARRFIKLDAQRKRLSPGSKEYQNVHEEVLQEYQKIKQSS 86 Query: 66 MREHSEHFKAKF 77 H E ++ ++ Sbjct: 87 PNYHEEKYRCEY 98 >1LHT-A nrPDB Length = 153 Score = 24.7 bits (52), Expect = 8.9, Method: Composition-based stats. Identities = 11/49 (22%), Positives = 25/49 (51%) Query: 40 LSPEMKEHYEKFEKMIQEHTDKFNKKMREHSEHFKAKFAELLEQQKNAQ 88 L PE +E + KF+ + K ++++++H +L+Q+ N + Sbjct: 35 LHPETQERFAKFKNLTTIDALKSSEEVKKHGTTVLTALGRILKQKNNHE 83 >2WL8-A nrPDB Length = 126 Score = 24.7 bits (52), Expect = 9.4, Method: Composition-based stats. Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 11/61 (18%) Query: 40 LSPEMKEHYEKFEKMIQEH-----TDKFNKKMREHS------EHFKAKFAELLEQQKNAQ 88 L P +KE EK+ + +Q H ++F K +HS E F+A+ E + A+ Sbjct: 31 LYPSLKEITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCKICEQFEAETPTDSETTQKAR 90 Query: 89 F 89 F Sbjct: 91 F 91 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.127 0.348 Lambda K H 0.267 0.0398 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,494,900 Number of Sequences: 36641 Number of extensions: 117440 Number of successful extensions: 472 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 48 Number of HSP's successfully gapped in prelim test: 30 Number of HSP's that attempted gapping in prelim test: 401 Number of HSP's gapped (non-prelim): 88 length of query: 92 length of database: 10,336,785 effective HSP length: 60 effective length of query: 32 effective length of database: 8,138,325 effective search space: 260426400 effective search space used: 260426400 T: 11 A: 40 X1: 15 ( 6.8 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 52 (24.7 bits)