BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P63172 (113 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5JPW-A nrPDB 236 2e-63 5WI4-A nrPDB 228 6e-61 3FM7-A nrPDB 169 2e-43 1XDX-A nrPDB 155 3e-39 5U2G-A nrPDB 32 0.080 3TW5-A nrPDB 27 1.7 3A9Z-A nrPDB 27 1.8 5OHS-A nrPDB 27 2.6 1RQX-A nrPDB 26 4.8 2CHR-A nrPDB 25 5.0 5KXJ-A nrPDB 25 5.4 3JRU-B nrPDB 25 7.1 6MRT-A nrPDB 25 7.2 1KNR-A nrPDB 25 7.6 4Z9M-A nrPDB 25 8.7 >5JPW-A nrPDB Length = 143 Score = 236 bits (602), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 113/113 (100%), Positives = 113/113 (100%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP Sbjct: 3 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 62 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI Sbjct: 63 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 115 >5WI4-A nrPDB Length = 145 Score = 228 bits (580), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 106/113 (93%), Positives = 112/113 (99%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 MED+QA+EETAFVVDEVS+IVKEAIESAIGGNAYQHSKVNQWTTNV+EQTLSQLTKLG+P Sbjct: 3 MEDFQASEETAFVVDEVSSIVKEAIESAIGGNAYQHSKVNQWTTNVLEQTLSQLTKLGRP 62 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 FKYIVTCVIMQKNGAGLH+ASSCFWDSSTDGSCTVRWENKTMYCIVS FGLSI Sbjct: 63 FKYIVTCVIMQKNGAGLHSASSCFWDSSTDGSCTVRWENKTMYCIVSTFGLSI 115 >3FM7-A nrPDB Length = 111 Score = 169 bits (429), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 79/113 (69%), Positives = 94/113 (83%), Gaps = 2/113 (1%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 M+D + EE+ F+VD+VS +KEAIE+ IGGNAYQH KVN WT VVE L+ LTK KP Sbjct: 1 MDD--SREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 +KYIVT +IMQKNGAGLHTASSC+W++ TDGSCTVRWENKTMYCIVS FGL++ Sbjct: 59 YKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 >1XDX-A nrPDB Length = 114 Score = 155 bits (392), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 68/108 (62%), Positives = 89/108 (82%) Query: 6 AAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIV 65 A EE AFV D+VSNI+KE+I++ + Y +KV+QWT++ +E + +LT L KPFKY+V Sbjct: 7 AVEEAAFVADDVSNIIKESIDAVLQNQQYSEAKVSQWTSSCLEHCIKRLTALNKPFKYVV 66 Query: 66 TCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 TC+IMQKNGAGLHTA+SC+WDS+TDGS TVRWENK+MYCI + FGL+I Sbjct: 67 TCIIMQKNGAGLHTAASCWWDSTTDGSRTVRWENKSMYCICTVFGLAI 114 >5U2G-A nrPDB Length = 838 Score = 31.6 bits (70), Expect = 0.080, Method: Composition-based stats. Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 5/40 (12%) Query: 24 AIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKY 63 AIE+ +GG +Y+ SK N+ T Q+L Q+ KPF Y Sbjct: 399 AIEAVVGGFSYEQSKFNRAT-----QSLVQVGSSIKPFIY 433 >3TW5-A nrPDB Length = 367 Score = 27.3 bits (59), Expect = 1.7, Method: Compositional matrix adjust. Identities = 26/85 (30%), Positives = 35/85 (41%), Gaps = 7/85 (8%) Query: 34 YQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTA-SSCFWDSS---- 88 YQ S QW+ GK K + V +KNG + C D Sbjct: 45 YQDSINVQWSQGQPSAAEKYAKAFGKDVKTFMDAV-SKKNGIDSQSGRKKCSSDDDCSTL 103 Query: 89 TDGS-CTVRWENKTMYCIVSAFGLS 112 TDGS C++R + YCI + FG+S Sbjct: 104 TDGSSCSIRTGKTSGYCIPTWFGIS 128 >3A9Z-A nrPDB Length = 432 Score = 26.9 bits (58), Expect = 1.8, Method: Composition-based stats. Identities = 24/89 (26%), Positives = 39/89 (43%), Gaps = 6/89 (6%) Query: 3 DYQAAEETAFVVDEVSNIVKEAIESAIG--GNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 DY A T + EV V EA++ A G ++Y + + N +L+++ GKP Sbjct: 23 DYNA---TTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIG-GKP 78 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSST 89 I T + N +H+ CF + T Sbjct: 79 QDIIFTSGGTESNNLVIHSTVRCFHEQQT 107 >5OHS-A nrPDB Length = 672 Score = 26.6 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 10/30 (33%), Positives = 18/30 (60%) Query: 15 DEVSNIVKEAIESAIGGNAYQHSKVNQWTT 44 D + ++ A+ S + GNAY HS + +T+ Sbjct: 464 DGLVTVICGALSSGLMGNAYHHSDIGGYTS 493 >1RQX-A nrPDB Length = 338 Score = 25.8 bits (55), Expect = 4.8, Method: Compositional matrix adjust. Identities = 18/65 (27%), Positives = 27/65 (41%), Gaps = 9/65 (13%) Query: 22 KEAIES--AIGGNAY-------QHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQK 72 ++A+ES A GG Y H E+ +Q +LG F Y+V C + Sbjct: 142 EDALESVRAAGGKPYAIPAGCSDHPLGGLGFVGFAEEVRAQEAELGFKFDYVVVCSVTGS 201 Query: 73 NGAGL 77 AG+ Sbjct: 202 TQAGM 206 >2CHR-A nrPDB Length = 370 Score = 25.4 bits (54), Expect = 5.0, Method: Composition-based stats. Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Query: 23 EAIESAIGGNAYQHSKVNQ-WTTNVVEQTLSQLTKLG 58 EA+ +++G AY VNQ W V + +L LG Sbjct: 179 EALSNSLGSKAYLRVDVNQAWDEQVASVYIPELEALG 215 >5KXJ-A nrPDB Length = 557 Score = 25.4 bits (54), Expect = 5.4, Method: Composition-based stats. Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 1/55 (1%) Query: 25 IESAIGGNAYQHSKVNQWTTNV-VEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLH 78 +E A+ +++++ N V L +L L + + IV C +M+K GLH Sbjct: 460 LERALRRITMLQQEIDEYYANFRVSNNLLELRNLVQVAELIVRCAMMRKESRGLH 514 >3JRU-B nrPDB Length = 490 Score = 25.0 bits (53), Expect = 7.1, Method: Composition-based stats. Identities = 11/20 (55%), Positives = 15/20 (75%) Query: 20 IVKEAIESAIGGNAYQHSKV 39 +V A+E+AI GNAY+ S V Sbjct: 310 VVVPAVENAIDGNAYRPSDV 329 >6MRT-A nrPDB Length = 324 Score = 25.0 bits (53), Expect = 7.2, Method: Compositional matrix adjust. Identities = 16/64 (25%), Positives = 33/64 (51%), Gaps = 5/64 (7%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQ---WTTNVVEQTLSQLTKL 57 +++ A T V D+ +VK AIE+A G + ++Q W T ++T+ +L++ Sbjct: 14 LDEVDAGSMTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQT--FDETIKELSRF 71 Query: 58 GKPF 61 + + Sbjct: 72 KQEY 75 >1KNR-A nrPDB Length = 540 Score = 25.0 bits (53), Expect = 7.6, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 17/32 (53%) Query: 47 VEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLH 78 V L +L L + + IV C +M+K GLH Sbjct: 483 VSNNLLELRNLVQVAELIVRCAMMRKESRGLH 514 >4Z9M-A nrPDB Length = 392 Score = 24.6 bits (52), Expect = 8.7, Method: Composition-based stats. Identities = 14/58 (24%), Positives = 28/58 (48%) Query: 16 EVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKN 73 EV N+ A+E G A ++ K+++ T ++ + KP ++TC M ++ Sbjct: 163 EVENVAITALEGLKGDLAGRYYKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARD 220 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5JPW-A nrPDB 204 5e-54 5WI4-A nrPDB 198 4e-52 3FM7-A nrPDB 181 5e-47 1XDX-A nrPDB 179 2e-46 Sequences not found previously or not previously below threshold: 3TW5-A nrPDB 29 0.35 5U2G-A nrPDB 29 0.44 6MRT-A nrPDB 27 2.0 5ZRV-A nrPDB 26 3.1 6N8J-m nrPDB 26 3.7 5OHS-A nrPDB 25 7.4 3EBY-A nrPDB 25 9.8 CONVERGED! >5JPW-A nrPDB Length = 143 Score = 204 bits (520), Expect = 5e-54, Method: Composition-based stats. Identities = 113/113 (100%), Positives = 113/113 (100%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP Sbjct: 3 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 62 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI Sbjct: 63 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 115 >5WI4-A nrPDB Length = 145 Score = 198 bits (504), Expect = 4e-52, Method: Composition-based stats. Identities = 106/113 (93%), Positives = 112/113 (99%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 MED+QA+EETAFVVDEVS+IVKEAIESAIGGNAYQHSKVNQWTTNV+EQTLSQLTKLG+P Sbjct: 3 MEDFQASEETAFVVDEVSSIVKEAIESAIGGNAYQHSKVNQWTTNVLEQTLSQLTKLGRP 62 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 FKYIVTCVIMQKNGAGLH+ASSCFWDSSTDGSCTVRWENKTMYCIVS FGLSI Sbjct: 63 FKYIVTCVIMQKNGAGLHSASSCFWDSSTDGSCTVRWENKTMYCIVSTFGLSI 115 >3FM7-A nrPDB Length = 111 Score = 181 bits (460), Expect = 5e-47, Method: Composition-based stats. Identities = 79/113 (69%), Positives = 94/113 (83%), Gaps = 2/113 (1%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 60 M+D + EE+ F+VD+VS +KEAIE+ IGGNAYQH KVN WT VVE L+ LTK KP Sbjct: 1 MDD--SREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 Query: 61 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 +KYIVT +IMQKNGAGLHTASSC+W++ TDGSCTVRWENKTMYCIVS FGL++ Sbjct: 59 YKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 >1XDX-A nrPDB Length = 114 Score = 179 bits (454), Expect = 2e-46, Method: Composition-based stats. Identities = 68/108 (62%), Positives = 89/108 (82%) Query: 6 AAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIV 65 A EE AFV D+VSNI+KE+I++ + Y +KV+QWT++ +E + +LT L KPFKY+V Sbjct: 7 AVEEAAFVADDVSNIIKESIDAVLQNQQYSEAKVSQWTSSCLEHCIKRLTALNKPFKYVV 66 Query: 66 TCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI 113 TC+IMQKNGAGLHTA+SC+WDS+TDGS TVRWENK+MYCI + FGL+I Sbjct: 67 TCIIMQKNGAGLHTAASCWWDSTTDGSRTVRWENKSMYCICTVFGLAI 114 >3TW5-A nrPDB Length = 367 Score = 29.3 bits (64), Expect = 0.35, Method: Composition-based stats. Identities = 26/85 (30%), Positives = 35/85 (41%), Gaps = 7/85 (8%) Query: 34 YQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTA-SSCFWDSS---- 88 YQ S QW+ GK K + V +KNG + C D Sbjct: 45 YQDSINVQWSQGQPSAAEKYAKAFGKDVKTFMDAV-SKKNGIDSQSGRKKCSSDDDCSTL 103 Query: 89 TDGS-CTVRWENKTMYCIVSAFGLS 112 TDGS C++R + YCI + FG+S Sbjct: 104 TDGSSCSIRTGKTSGYCIPTWFGIS 128 >5U2G-A nrPDB Length = 838 Score = 29.3 bits (64), Expect = 0.44, Method: Composition-based stats. Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 5/40 (12%) Query: 24 AIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKY 63 AIE+ +GG +Y+ SK N+ T Q+L Q+ KPF Y Sbjct: 399 AIEAVVGGFSYEQSKFNRAT-----QSLVQVGSSIKPFIY 433 >6MRT-A nrPDB Length = 324 Score = 27.0 bits (58), Expect = 2.0, Method: Composition-based stats. Identities = 17/73 (23%), Positives = 35/73 (47%), Gaps = 7/73 (9%) Query: 1 MEDYQAAEETAFVVDEVSNIVKEAIESAIGG----NAYQHSKVNQWTTNVVEQTLSQLTK 56 +++ A T V D+ +VK AIE+A G N Y +V W ++T+ +L++ Sbjct: 14 LDEVDAGSMTEIVADKTVEVVKNAIETADGALDLYNKYL-DQVIPW--QTFDETIKELSR 70 Query: 57 LGKPFKYIVTCVI 69 + + + ++ Sbjct: 71 FKQEYSQAASVLV 83 >5ZRV-A nrPDB Length = 763 Score = 26.2 bits (56), Expect = 3.1, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 26/64 (40%) Query: 32 NAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTASSCFWDSSTDG 91 N K Q T + ++ + KL K K IV + G GL A SC + +T Sbjct: 105 NMLAACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKD 164 Query: 92 SCTV 95 TV Sbjct: 165 RKTV 168 >6N8J-m nrPDB Length = 486 Score = 26.2 bits (56), Expect = 3.7, Method: Composition-based stats. Identities = 9/32 (28%), Positives = 16/32 (50%) Query: 22 KEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQ 53 K+ +++ +G Q K N WT+ E S+ Sbjct: 174 KQVLDATLGLMGNQEDKENGWTSAAKEAIFSK 205 >5OHS-A nrPDB Length = 672 Score = 25.0 bits (53), Expect = 7.4, Method: Composition-based stats. Identities = 10/30 (33%), Positives = 18/30 (60%) Query: 15 DEVSNIVKEAIESAIGGNAYQHSKVNQWTT 44 D + ++ A+ S + GNAY HS + +T+ Sbjct: 464 DGLVTVICGALSSGLMGNAYHHSDIGGYTS 493 >3EBY-A nrPDB Length = 163 Score = 24.7 bits (52), Expect = 9.8, Method: Composition-based stats. Identities = 13/46 (28%), Positives = 22/46 (47%), Gaps = 2/46 (4%) Query: 13 VVDEVSNIVKEAIE--SAIGGNAYQHSKVNQWTTNVVEQTLSQLTK 56 V EV+ + + AI+ +A G ++ QW T V+ L Q+ Sbjct: 4 VAAEVAQVAQSAIDDFNAAYGLCLDDDRLEQWPTLFVDDCLYQVIA 49 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.132 0.408 Lambda K H 0.267 0.0405 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,380,817 Number of Sequences: 36641 Number of extensions: 146720 Number of successful extensions: 407 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 24 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 383 Number of HSP's gapped (non-prelim): 37 length of query: 113 length of database: 10,336,785 effective HSP length: 79 effective length of query: 34 effective length of database: 7,442,146 effective search space: 253032964 effective search space used: 253032964 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.7 bits)