BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P61917
(151 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5KWY-C nrPDB 270 2e-73
1NEP-A nrPDB 225 6e-60
6F73-A nrPDB 34 0.022
5UXZ-A nrPDB 27 4.3
4OER-A nrPDB 27 4.6
2Z2O-A nrPDB 26 5.4
1A9V-A nrPDB 26 8.0
>5KWY-C nrPDB
Length = 133
Score = 270 bits (689), Expect = 2e-73, Method: Compositional matrix adjust.
Identities = 132/132 (100%), Positives = 132/132 (100%)
Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM
Sbjct: 1 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 60
Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF
Sbjct: 61 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 120
Query: 140 CWEIPVQIVSHL 151
CWEIPVQIVSHL
Sbjct: 121 CWEIPVQIVSHL 132
>1NEP-A nrPDB
Length = 130
Score = 225 bits (573), Expect = 6e-60, Method: Compositional matrix adjust.
Identities = 103/128 (80%), Positives = 119/128 (92%)
Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
EPV+FKDCGS GVIKEVNVSPCPTQPC+L +GQSYSVNVTFTSN QS+SSKAVVHGI+M
Sbjct: 1 EPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVM 60
Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
G+PVPFPIPE DGCKSGI CPI+KDKTY+Y+NKLPVK+EYPSIK+VVEW+L DDKNQ F
Sbjct: 61 GIPVPFPIPESDGCKSGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFF 120
Query: 140 CWEIPVQI 147
CW+IP+++
Sbjct: 121 CWQIPIEV 128
>6F73-A nrPDB
Length = 574
Score = 34.3 bits (77), Expect = 0.022, Method: Composition-based stats.
Identities = 15/35 (42%), Positives = 21/35 (60%)
Query: 43 PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77
P+QPCQL SY VNVT +++Q+ A H +
Sbjct: 114 PSQPCQLGNYPSYVVNVTGAADVQAALKFAQKHNV 148
>5UXZ-A nrPDB
Length = 307
Score = 26.6 bits (57), Expect = 4.3, Method: Compositional matrix adjust.
Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 9/91 (9%)
Query: 62 TSNIQSK---SSKAVVHGIL-MGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKS 117
T+++ K + G L MGV P E D + P+ ++ + + N+LP+
Sbjct: 151 TTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAG 210
Query: 118 EYPSIKLVVEWQLQDDKNQS----LFCWEIP 144
E +I +VE + D +QS L W P
Sbjct: 211 EPANIVALVE-EYMDWLHQSPVPKLLFWGTP 240
>4OER-A nrPDB
Length = 500
Score = 26.6 bits (57), Expect = 4.6, Method: Composition-based stats.
Identities = 10/41 (24%), Positives = 21/41 (51%)
Query: 21 PVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTF 61
P QFK+ G+ DG++ + P +L + ++ N ++
Sbjct: 142 PSQFKNGGTADGIVAPIGTGPWKLTETKLGEHDVFTRNDSY 182
>2Z2O-A nrPDB
Length = 299
Score = 26.2 bits (56), Expect = 5.4, Method: Compositional matrix adjust.
Identities = 32/115 (27%), Positives = 42/115 (36%), Gaps = 30/115 (26%)
Query: 27 CGSVDGVIKEVNVSPCPTQPCQL------SKGQSYSVNVTFTSNIQSKSSKAVVHGILMG 80
C ++DG I E P PT ++ S G+ V FT N +K + GI+
Sbjct: 40 CINLDGKITEY---PLPTPDAKVMCLTISSDGE-----VWFTENAANKIGRITKKGIIKE 91
Query: 81 VPVPFPIPEPDGCKSGINCPI--------------QKDKTYSYLNKLPVKSEYPS 121
+P P P G G N I K Y +LP K YPS
Sbjct: 92 YTLPNPDSAPYGITEGPNGDIWFTEMNGNRIGRITDDGKIREY--ELPNKGSYPS 144
>1A9V-A nrPDB
Length = 129
Score = 25.8 bits (55), Expect = 8.0, Method: Compositional matrix adjust.
Identities = 21/59 (35%), Positives = 28/59 (47%), Gaps = 8/59 (13%)
Query: 28 GSVDGVIKEVNV-----SPCPTQPCQLSKGQSYSVNVTFTS-NIQSKSSKAVVHGILMG 80
S+DG+ EV+V + C C L KGQ Y + T+ I KS VV +MG
Sbjct: 56 ASIDGL--EVDVPGIDPNACHYMKCPLVKGQQYDIKYTWNVPKIAPKSENVVVTVKVMG 112
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
1NEP-A nrPDB 254 8e-69
5KWY-C nrPDB 254 1e-68
Sequences not found previously or not previously below threshold:
1A9V-A nrPDB 48 1e-06
6F73-A nrPDB 34 0.027
5UXZ-A nrPDB 28 1.3
1HDG-O nrPDB 27 2.4
2Z2O-A nrPDB 27 3.7
3QJ3-A nrPDB 26 5.3
1JMU-B nrPDB 26 6.7
>1NEP-A nrPDB
Length = 130
Score = 254 bits (650), Expect = 8e-69, Method: Composition-based stats.
Identities = 103/128 (80%), Positives = 119/128 (92%)
Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
EPV+FKDCGS GVIKEVNVSPCPTQPC+L +GQSYSVNVTFTSN QS+SSKAVVHGI+M
Sbjct: 1 EPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVM 60
Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
G+PVPFPIPE DGCKSGI CPI+KDKTY+Y+NKLPVK+EYPSIK+VVEW+L DDKNQ F
Sbjct: 61 GIPVPFPIPESDGCKSGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFF 120
Query: 140 CWEIPVQI 147
CW+IP+++
Sbjct: 121 CWQIPIEV 128
>5KWY-C nrPDB
Length = 133
Score = 254 bits (648), Expect = 1e-68, Method: Composition-based stats.
Identities = 132/132 (100%), Positives = 132/132 (100%)
Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM
Sbjct: 1 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 60
Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF
Sbjct: 61 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 120
Query: 140 CWEIPVQIVSHL 151
CWEIPVQIVSHL
Sbjct: 121 CWEIPVQIVSHL 132
>1A9V-A nrPDB
Length = 129
Score = 48.1 bits (113), Expect = 1e-06, Method: Composition-based stats.
Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 4/88 (4%)
Query: 22 VQFKDCGSVDGVIKEVNVSPC-PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMG 80
V KDC + + IK+V V C ++PC + +G+ + + F +N +K++K + + G
Sbjct: 3 VDVKDCANHE--IKKVLVPGCHGSEPCIIHRGKPFQLEAVFEANQNTKTAKIEIKASIDG 60
Query: 81 VPVPFPIPEPDGCKSGINCPIQKDKTYS 108
+ V P +P+ C + CP+ K + Y
Sbjct: 61 LEVDVPGIDPNACHY-MKCPLVKGQQYD 87
>6F73-A nrPDB
Length = 574
Score = 33.9 bits (76), Expect = 0.027, Method: Composition-based stats.
Identities = 15/35 (42%), Positives = 21/35 (60%)
Query: 43 PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77
P+QPCQL SY VNVT +++Q+ A H +
Sbjct: 114 PSQPCQLGNYPSYVVNVTGAADVQAALKFAQKHNV 148
>5UXZ-A nrPDB
Length = 307
Score = 28.5 bits (62), Expect = 1.3, Method: Composition-based stats.
Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 5/70 (7%)
Query: 79 MGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVE----WQLQDDK 134
MGV P E D + P+ ++ + + N+LP+ E +I +VE W Q
Sbjct: 172 MGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV 231
Query: 135 NQSLFCWEIP 144
+ LF W P
Sbjct: 232 PKLLF-WGTP 240
>1HDG-O nrPDB
Length = 332
Score = 27.3 bits (59), Expect = 2.4, Method: Composition-based stats.
Identities = 17/68 (25%), Positives = 35/68 (51%), Gaps = 5/68 (7%)
Query: 49 LSKGQSYSVNVTFTSNIQSKSSKAVV---HGILMGVPVPFPIPEPDGCKSGINCPIQKDK 105
L + ++ +VN+ T+ +K+ VV G L G+ + +P PDG + + ++K+
Sbjct: 194 LRRARAAAVNIIPTTTGAAKAVALVVPEVKGKLDGMAIR--VPTPDGSITDLTVLVEKET 251
Query: 106 TYSYLNKL 113
T +N +
Sbjct: 252 TVEEVNAV 259
>2Z2O-A nrPDB
Length = 299
Score = 26.9 bits (58), Expect = 3.7, Method: Composition-based stats.
Identities = 31/110 (28%), Positives = 40/110 (36%), Gaps = 20/110 (18%)
Query: 27 CGSVDGVIKEVNVSPCPTQPCQ-LSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPF 85
C ++DG I E P PT + + S V FT N +K + GI+ +P
Sbjct: 40 CINLDGKITEY---PLPTPDAKVMCLTISSDGEVWFTENAANKIGRITKKGIIKEYTLPN 96
Query: 86 PIPEPDGCKSGINCPI--------------QKDKTYSYLNKLPVKSEYPS 121
P P G G N I K Y +LP K YPS
Sbjct: 97 PDSAPYGITEGPNGDIWFTEMNGNRIGRITDDGKIREY--ELPNKGSYPS 144
>3QJ3-A nrPDB
Length = 331
Score = 26.1 bits (56), Expect = 5.3, Method: Composition-based stats.
Identities = 16/71 (22%), Positives = 32/71 (45%), Gaps = 8/71 (11%)
Query: 54 SYSVNVTFTSNIQSKSSKAVVHGILMGVP-----VPFPIPEPDGCKSGINCPIQKD-KTY 107
SY++ V +++ + KA HG++M +P E G + + P D +
Sbjct: 66 SYTLGVNLFTDMTPEEMKAYTHGLIMPADLHKNGIPIKTREDLGLNASVRYPASFDWRDQ 125
Query: 108 SYLNKLPVKSE 118
++ PVK++
Sbjct: 126 GMVS--PVKNQ 134
>1JMU-B nrPDB
Length = 666
Score = 26.1 bits (56), Expect = 6.7, Method: Composition-based stats.
Identities = 23/87 (26%), Positives = 31/87 (35%), Gaps = 15/87 (17%)
Query: 18 QAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77
QA PV D + + + V S +K S V + S+S + G
Sbjct: 571 QAAPV-LSDKNNWIALAQGVKTS-------LRTKSLSAGVKTAVSKLSSSESIQNWTQGF 622
Query: 78 LMGVPVPFPIPEPDGCKSGINCPIQKD 104
L V FP P+PD CP D
Sbjct: 623 LDKVSTHFPAPKPD-------CPTNGD 642
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.311 0.137 0.434
Lambda K H
0.267 0.0399 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,312,975
Number of Sequences: 36641
Number of extensions: 260568
Number of successful extensions: 664
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 649
Number of HSP's gapped (non-prelim): 27
length of query: 151
length of database: 10,336,785
effective HSP length: 88
effective length of query: 63
effective length of database: 7,112,377
effective search space: 448079751
effective search space used: 448079751
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 54 (25.4 bits)