BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P61917
         (151 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

5KWY-C nrPDB                                                          270   2e-73
1NEP-A nrPDB                                                          225   6e-60
6F73-A nrPDB                                                           34   0.022
5UXZ-A nrPDB                                                           27   4.3  
4OER-A nrPDB                                                           27   4.6  
2Z2O-A nrPDB                                                           26   5.4  
1A9V-A nrPDB                                                           26   8.0  

>5KWY-C nrPDB
          Length = 133

 Score =  270 bits (689), Expect = 2e-73,   Method: Compositional matrix adjust.
 Identities = 132/132 (100%), Positives = 132/132 (100%)

Query: 20  EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
           EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM
Sbjct: 1   EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 60

Query: 80  GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
           GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF
Sbjct: 61  GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 120

Query: 140 CWEIPVQIVSHL 151
           CWEIPVQIVSHL
Sbjct: 121 CWEIPVQIVSHL 132


>1NEP-A nrPDB
          Length = 130

 Score =  225 bits (573), Expect = 6e-60,   Method: Compositional matrix adjust.
 Identities = 103/128 (80%), Positives = 119/128 (92%)

Query: 20  EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
           EPV+FKDCGS  GVIKEVNVSPCPTQPC+L +GQSYSVNVTFTSN QS+SSKAVVHGI+M
Sbjct: 1   EPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVM 60

Query: 80  GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
           G+PVPFPIPE DGCKSGI CPI+KDKTY+Y+NKLPVK+EYPSIK+VVEW+L DDKNQ  F
Sbjct: 61  GIPVPFPIPESDGCKSGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFF 120

Query: 140 CWEIPVQI 147
           CW+IP+++
Sbjct: 121 CWQIPIEV 128


>6F73-A nrPDB
          Length = 574

 Score = 34.3 bits (77), Expect = 0.022,   Method: Composition-based stats.
 Identities = 15/35 (42%), Positives = 21/35 (60%)

Query: 43  PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77
           P+QPCQL    SY VNVT  +++Q+    A  H +
Sbjct: 114 PSQPCQLGNYPSYVVNVTGAADVQAALKFAQKHNV 148


>5UXZ-A nrPDB
          Length = 307

 Score = 26.6 bits (57), Expect = 4.3,   Method: Compositional matrix adjust.
 Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 9/91 (9%)

Query: 62  TSNIQSK---SSKAVVHGIL-MGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKS 117
           T+++  K        + G L MGV  P    E D  +     P+ ++  + + N+LP+  
Sbjct: 151 TTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAG 210

Query: 118 EYPSIKLVVEWQLQDDKNQS----LFCWEIP 144
           E  +I  +VE +  D  +QS    L  W  P
Sbjct: 211 EPANIVALVE-EYMDWLHQSPVPKLLFWGTP 240


>4OER-A nrPDB
          Length = 500

 Score = 26.6 bits (57), Expect = 4.6,   Method: Composition-based stats.
 Identities = 10/41 (24%), Positives = 21/41 (51%)

Query: 21  PVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTF 61
           P QFK+ G+ DG++  +   P      +L +   ++ N ++
Sbjct: 142 PSQFKNGGTADGIVAPIGTGPWKLTETKLGEHDVFTRNDSY 182


>2Z2O-A nrPDB
          Length = 299

 Score = 26.2 bits (56), Expect = 5.4,   Method: Compositional matrix adjust.
 Identities = 32/115 (27%), Positives = 42/115 (36%), Gaps = 30/115 (26%)

Query: 27  CGSVDGVIKEVNVSPCPTQPCQL------SKGQSYSVNVTFTSNIQSKSSKAVVHGILMG 80
           C ++DG I E    P PT   ++      S G+     V FT N  +K  +    GI+  
Sbjct: 40  CINLDGKITEY---PLPTPDAKVMCLTISSDGE-----VWFTENAANKIGRITKKGIIKE 91

Query: 81  VPVPFPIPEPDGCKSGINCPI--------------QKDKTYSYLNKLPVKSEYPS 121
             +P P   P G   G N  I                 K   Y  +LP K  YPS
Sbjct: 92  YTLPNPDSAPYGITEGPNGDIWFTEMNGNRIGRITDDGKIREY--ELPNKGSYPS 144


>1A9V-A nrPDB
          Length = 129

 Score = 25.8 bits (55), Expect = 8.0,   Method: Compositional matrix adjust.
 Identities = 21/59 (35%), Positives = 28/59 (47%), Gaps = 8/59 (13%)

Query: 28  GSVDGVIKEVNV-----SPCPTQPCQLSKGQSYSVNVTFTS-NIQSKSSKAVVHGILMG 80
            S+DG+  EV+V     + C    C L KGQ Y +  T+    I  KS   VV   +MG
Sbjct: 56  ASIDGL--EVDVPGIDPNACHYMKCPLVKGQQYDIKYTWNVPKIAPKSENVVVTVKVMG 112


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

1NEP-A nrPDB                                                          254   8e-69
5KWY-C nrPDB                                                          254   1e-68

Sequences not found previously or not previously below threshold:

1A9V-A nrPDB                                                           48   1e-06
6F73-A nrPDB                                                           34   0.027
5UXZ-A nrPDB                                                           28   1.3  
1HDG-O nrPDB                                                           27   2.4  
2Z2O-A nrPDB                                                           27   3.7  
3QJ3-A nrPDB                                                           26   5.3  
1JMU-B nrPDB                                                           26   6.7  

>1NEP-A nrPDB
          Length = 130

 Score =  254 bits (650), Expect = 8e-69,   Method: Composition-based stats.
 Identities = 103/128 (80%), Positives = 119/128 (92%)

Query: 20  EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
           EPV+FKDCGS  GVIKEVNVSPCPTQPC+L +GQSYSVNVTFTSN QS+SSKAVVHGI+M
Sbjct: 1   EPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVM 60

Query: 80  GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
           G+PVPFPIPE DGCKSGI CPI+KDKTY+Y+NKLPVK+EYPSIK+VVEW+L DDKNQ  F
Sbjct: 61  GIPVPFPIPESDGCKSGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFF 120

Query: 140 CWEIPVQI 147
           CW+IP+++
Sbjct: 121 CWQIPIEV 128


>5KWY-C nrPDB
          Length = 133

 Score =  254 bits (648), Expect = 1e-68,   Method: Composition-based stats.
 Identities = 132/132 (100%), Positives = 132/132 (100%)

Query: 20  EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79
           EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM
Sbjct: 1   EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 60

Query: 80  GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139
           GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF
Sbjct: 61  GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 120

Query: 140 CWEIPVQIVSHL 151
           CWEIPVQIVSHL
Sbjct: 121 CWEIPVQIVSHL 132


>1A9V-A nrPDB
          Length = 129

 Score = 48.1 bits (113), Expect = 1e-06,   Method: Composition-based stats.
 Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 4/88 (4%)

Query: 22  VQFKDCGSVDGVIKEVNVSPC-PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMG 80
           V  KDC + +  IK+V V  C  ++PC + +G+ + +   F +N  +K++K  +   + G
Sbjct: 3   VDVKDCANHE--IKKVLVPGCHGSEPCIIHRGKPFQLEAVFEANQNTKTAKIEIKASIDG 60

Query: 81  VPVPFPIPEPDGCKSGINCPIQKDKTYS 108
           + V  P  +P+ C   + CP+ K + Y 
Sbjct: 61  LEVDVPGIDPNACHY-MKCPLVKGQQYD 87


>6F73-A nrPDB
          Length = 574

 Score = 33.9 bits (76), Expect = 0.027,   Method: Composition-based stats.
 Identities = 15/35 (42%), Positives = 21/35 (60%)

Query: 43  PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77
           P+QPCQL    SY VNVT  +++Q+    A  H +
Sbjct: 114 PSQPCQLGNYPSYVVNVTGAADVQAALKFAQKHNV 148


>5UXZ-A nrPDB
          Length = 307

 Score = 28.5 bits (62), Expect = 1.3,   Method: Composition-based stats.
 Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 5/70 (7%)

Query: 79  MGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVE----WQLQDDK 134
           MGV  P    E D  +     P+ ++  + + N+LP+  E  +I  +VE    W  Q   
Sbjct: 172 MGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV 231

Query: 135 NQSLFCWEIP 144
            + LF W  P
Sbjct: 232 PKLLF-WGTP 240


>1HDG-O nrPDB
          Length = 332

 Score = 27.3 bits (59), Expect = 2.4,   Method: Composition-based stats.
 Identities = 17/68 (25%), Positives = 35/68 (51%), Gaps = 5/68 (7%)

Query: 49  LSKGQSYSVNVTFTSNIQSKSSKAVV---HGILMGVPVPFPIPEPDGCKSGINCPIQKDK 105
           L + ++ +VN+  T+   +K+   VV    G L G+ +   +P PDG  + +   ++K+ 
Sbjct: 194 LRRARAAAVNIIPTTTGAAKAVALVVPEVKGKLDGMAIR--VPTPDGSITDLTVLVEKET 251

Query: 106 TYSYLNKL 113
           T   +N +
Sbjct: 252 TVEEVNAV 259


>2Z2O-A nrPDB
          Length = 299

 Score = 26.9 bits (58), Expect = 3.7,   Method: Composition-based stats.
 Identities = 31/110 (28%), Positives = 40/110 (36%), Gaps = 20/110 (18%)

Query: 27  CGSVDGVIKEVNVSPCPTQPCQ-LSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPF 85
           C ++DG I E    P PT   + +    S    V FT N  +K  +    GI+    +P 
Sbjct: 40  CINLDGKITEY---PLPTPDAKVMCLTISSDGEVWFTENAANKIGRITKKGIIKEYTLPN 96

Query: 86  PIPEPDGCKSGINCPI--------------QKDKTYSYLNKLPVKSEYPS 121
           P   P G   G N  I                 K   Y  +LP K  YPS
Sbjct: 97  PDSAPYGITEGPNGDIWFTEMNGNRIGRITDDGKIREY--ELPNKGSYPS 144


>3QJ3-A nrPDB
          Length = 331

 Score = 26.1 bits (56), Expect = 5.3,   Method: Composition-based stats.
 Identities = 16/71 (22%), Positives = 32/71 (45%), Gaps = 8/71 (11%)

Query: 54  SYSVNVTFTSNIQSKSSKAVVHGILMGVP-----VPFPIPEPDGCKSGINCPIQKD-KTY 107
           SY++ V   +++  +  KA  HG++M        +P    E  G  + +  P   D +  
Sbjct: 66  SYTLGVNLFTDMTPEEMKAYTHGLIMPADLHKNGIPIKTREDLGLNASVRYPASFDWRDQ 125

Query: 108 SYLNKLPVKSE 118
             ++  PVK++
Sbjct: 126 GMVS--PVKNQ 134


>1JMU-B nrPDB
          Length = 666

 Score = 26.1 bits (56), Expect = 6.7,   Method: Composition-based stats.
 Identities = 23/87 (26%), Positives = 31/87 (35%), Gaps = 15/87 (17%)

Query: 18  QAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77
           QA PV   D  +   + + V  S         +K  S  V    +    S+S +    G 
Sbjct: 571 QAAPV-LSDKNNWIALAQGVKTS-------LRTKSLSAGVKTAVSKLSSSESIQNWTQGF 622

Query: 78  LMGVPVPFPIPEPDGCKSGINCPIQKD 104
           L  V   FP P+PD       CP   D
Sbjct: 623 LDKVSTHFPAPKPD-------CPTNGD 642


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.311    0.137    0.434 

Lambda     K      H
   0.267   0.0399    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,312,975
Number of Sequences: 36641
Number of extensions: 260568
Number of successful extensions: 664
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 649
Number of HSP's gapped (non-prelim): 27
length of query: 151
length of database: 10,336,785
effective HSP length: 88
effective length of query: 63
effective length of database: 7,112,377
effective search space: 448079751
effective search space used: 448079751
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 54 (25.4 bits)