BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P61917 (151 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5KWY-C nrPDB 270 2e-73 1NEP-A nrPDB 225 6e-60 6F73-A nrPDB 34 0.022 5UXZ-A nrPDB 27 4.3 4OER-A nrPDB 27 4.6 2Z2O-A nrPDB 26 5.4 1A9V-A nrPDB 26 8.0 >5KWY-C nrPDB Length = 133 Score = 270 bits (689), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 132/132 (100%), Positives = 132/132 (100%) Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM Sbjct: 1 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 60 Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF Sbjct: 61 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 120 Query: 140 CWEIPVQIVSHL 151 CWEIPVQIVSHL Sbjct: 121 CWEIPVQIVSHL 132 >1NEP-A nrPDB Length = 130 Score = 225 bits (573), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 103/128 (80%), Positives = 119/128 (92%) Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79 EPV+FKDCGS GVIKEVNVSPCPTQPC+L +GQSYSVNVTFTSN QS+SSKAVVHGI+M Sbjct: 1 EPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVM 60 Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139 G+PVPFPIPE DGCKSGI CPI+KDKTY+Y+NKLPVK+EYPSIK+VVEW+L DDKNQ F Sbjct: 61 GIPVPFPIPESDGCKSGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFF 120 Query: 140 CWEIPVQI 147 CW+IP+++ Sbjct: 121 CWQIPIEV 128 >6F73-A nrPDB Length = 574 Score = 34.3 bits (77), Expect = 0.022, Method: Composition-based stats. Identities = 15/35 (42%), Positives = 21/35 (60%) Query: 43 PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77 P+QPCQL SY VNVT +++Q+ A H + Sbjct: 114 PSQPCQLGNYPSYVVNVTGAADVQAALKFAQKHNV 148 >5UXZ-A nrPDB Length = 307 Score = 26.6 bits (57), Expect = 4.3, Method: Compositional matrix adjust. Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 9/91 (9%) Query: 62 TSNIQSK---SSKAVVHGIL-MGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKS 117 T+++ K + G L MGV P E D + P+ ++ + + N+LP+ Sbjct: 151 TTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAG 210 Query: 118 EYPSIKLVVEWQLQDDKNQS----LFCWEIP 144 E +I +VE + D +QS L W P Sbjct: 211 EPANIVALVE-EYMDWLHQSPVPKLLFWGTP 240 >4OER-A nrPDB Length = 500 Score = 26.6 bits (57), Expect = 4.6, Method: Composition-based stats. Identities = 10/41 (24%), Positives = 21/41 (51%) Query: 21 PVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTF 61 P QFK+ G+ DG++ + P +L + ++ N ++ Sbjct: 142 PSQFKNGGTADGIVAPIGTGPWKLTETKLGEHDVFTRNDSY 182 >2Z2O-A nrPDB Length = 299 Score = 26.2 bits (56), Expect = 5.4, Method: Compositional matrix adjust. Identities = 32/115 (27%), Positives = 42/115 (36%), Gaps = 30/115 (26%) Query: 27 CGSVDGVIKEVNVSPCPTQPCQL------SKGQSYSVNVTFTSNIQSKSSKAVVHGILMG 80 C ++DG I E P PT ++ S G+ V FT N +K + GI+ Sbjct: 40 CINLDGKITEY---PLPTPDAKVMCLTISSDGE-----VWFTENAANKIGRITKKGIIKE 91 Query: 81 VPVPFPIPEPDGCKSGINCPI--------------QKDKTYSYLNKLPVKSEYPS 121 +P P P G G N I K Y +LP K YPS Sbjct: 92 YTLPNPDSAPYGITEGPNGDIWFTEMNGNRIGRITDDGKIREY--ELPNKGSYPS 144 >1A9V-A nrPDB Length = 129 Score = 25.8 bits (55), Expect = 8.0, Method: Compositional matrix adjust. Identities = 21/59 (35%), Positives = 28/59 (47%), Gaps = 8/59 (13%) Query: 28 GSVDGVIKEVNV-----SPCPTQPCQLSKGQSYSVNVTFTS-NIQSKSSKAVVHGILMG 80 S+DG+ EV+V + C C L KGQ Y + T+ I KS VV +MG Sbjct: 56 ASIDGL--EVDVPGIDPNACHYMKCPLVKGQQYDIKYTWNVPKIAPKSENVVVTVKVMG 112 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1NEP-A nrPDB 254 8e-69 5KWY-C nrPDB 254 1e-68 Sequences not found previously or not previously below threshold: 1A9V-A nrPDB 48 1e-06 6F73-A nrPDB 34 0.027 5UXZ-A nrPDB 28 1.3 1HDG-O nrPDB 27 2.4 2Z2O-A nrPDB 27 3.7 3QJ3-A nrPDB 26 5.3 1JMU-B nrPDB 26 6.7 >1NEP-A nrPDB Length = 130 Score = 254 bits (650), Expect = 8e-69, Method: Composition-based stats. Identities = 103/128 (80%), Positives = 119/128 (92%) Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79 EPV+FKDCGS GVIKEVNVSPCPTQPC+L +GQSYSVNVTFTSN QS+SSKAVVHGI+M Sbjct: 1 EPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVM 60 Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139 G+PVPFPIPE DGCKSGI CPI+KDKTY+Y+NKLPVK+EYPSIK+VVEW+L DDKNQ F Sbjct: 61 GIPVPFPIPESDGCKSGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFF 120 Query: 140 CWEIPVQI 147 CW+IP+++ Sbjct: 121 CWQIPIEV 128 >5KWY-C nrPDB Length = 133 Score = 254 bits (648), Expect = 1e-68, Method: Composition-based stats. Identities = 132/132 (100%), Positives = 132/132 (100%) Query: 20 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 79 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM Sbjct: 1 EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILM 60 Query: 80 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 139 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF Sbjct: 61 GVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLF 120 Query: 140 CWEIPVQIVSHL 151 CWEIPVQIVSHL Sbjct: 121 CWEIPVQIVSHL 132 >1A9V-A nrPDB Length = 129 Score = 48.1 bits (113), Expect = 1e-06, Method: Composition-based stats. Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 4/88 (4%) Query: 22 VQFKDCGSVDGVIKEVNVSPC-PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMG 80 V KDC + + IK+V V C ++PC + +G+ + + F +N +K++K + + G Sbjct: 3 VDVKDCANHE--IKKVLVPGCHGSEPCIIHRGKPFQLEAVFEANQNTKTAKIEIKASIDG 60 Query: 81 VPVPFPIPEPDGCKSGINCPIQKDKTYS 108 + V P +P+ C + CP+ K + Y Sbjct: 61 LEVDVPGIDPNACHY-MKCPLVKGQQYD 87 >6F73-A nrPDB Length = 574 Score = 33.9 bits (76), Expect = 0.027, Method: Composition-based stats. Identities = 15/35 (42%), Positives = 21/35 (60%) Query: 43 PTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77 P+QPCQL SY VNVT +++Q+ A H + Sbjct: 114 PSQPCQLGNYPSYVVNVTGAADVQAALKFAQKHNV 148 >5UXZ-A nrPDB Length = 307 Score = 28.5 bits (62), Expect = 1.3, Method: Composition-based stats. Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 5/70 (7%) Query: 79 MGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVE----WQLQDDK 134 MGV P E D + P+ ++ + + N+LP+ E +I +VE W Q Sbjct: 172 MGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV 231 Query: 135 NQSLFCWEIP 144 + LF W P Sbjct: 232 PKLLF-WGTP 240 >1HDG-O nrPDB Length = 332 Score = 27.3 bits (59), Expect = 2.4, Method: Composition-based stats. Identities = 17/68 (25%), Positives = 35/68 (51%), Gaps = 5/68 (7%) Query: 49 LSKGQSYSVNVTFTSNIQSKSSKAVV---HGILMGVPVPFPIPEPDGCKSGINCPIQKDK 105 L + ++ +VN+ T+ +K+ VV G L G+ + +P PDG + + ++K+ Sbjct: 194 LRRARAAAVNIIPTTTGAAKAVALVVPEVKGKLDGMAIR--VPTPDGSITDLTVLVEKET 251 Query: 106 TYSYLNKL 113 T +N + Sbjct: 252 TVEEVNAV 259 >2Z2O-A nrPDB Length = 299 Score = 26.9 bits (58), Expect = 3.7, Method: Composition-based stats. Identities = 31/110 (28%), Positives = 40/110 (36%), Gaps = 20/110 (18%) Query: 27 CGSVDGVIKEVNVSPCPTQPCQ-LSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPF 85 C ++DG I E P PT + + S V FT N +K + GI+ +P Sbjct: 40 CINLDGKITEY---PLPTPDAKVMCLTISSDGEVWFTENAANKIGRITKKGIIKEYTLPN 96 Query: 86 PIPEPDGCKSGINCPI--------------QKDKTYSYLNKLPVKSEYPS 121 P P G G N I K Y +LP K YPS Sbjct: 97 PDSAPYGITEGPNGDIWFTEMNGNRIGRITDDGKIREY--ELPNKGSYPS 144 >3QJ3-A nrPDB Length = 331 Score = 26.1 bits (56), Expect = 5.3, Method: Composition-based stats. Identities = 16/71 (22%), Positives = 32/71 (45%), Gaps = 8/71 (11%) Query: 54 SYSVNVTFTSNIQSKSSKAVVHGILMGVP-----VPFPIPEPDGCKSGINCPIQKD-KTY 107 SY++ V +++ + KA HG++M +P E G + + P D + Sbjct: 66 SYTLGVNLFTDMTPEEMKAYTHGLIMPADLHKNGIPIKTREDLGLNASVRYPASFDWRDQ 125 Query: 108 SYLNKLPVKSE 118 ++ PVK++ Sbjct: 126 GMVS--PVKNQ 134 >1JMU-B nrPDB Length = 666 Score = 26.1 bits (56), Expect = 6.7, Method: Composition-based stats. Identities = 23/87 (26%), Positives = 31/87 (35%), Gaps = 15/87 (17%) Query: 18 QAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGI 77 QA PV D + + + V S +K S V + S+S + G Sbjct: 571 QAAPV-LSDKNNWIALAQGVKTS-------LRTKSLSAGVKTAVSKLSSSESIQNWTQGF 622 Query: 78 LMGVPVPFPIPEPDGCKSGINCPIQKD 104 L V FP P+PD CP D Sbjct: 623 LDKVSTHFPAPKPD-------CPTNGD 642 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.137 0.434 Lambda K H 0.267 0.0399 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,312,975 Number of Sequences: 36641 Number of extensions: 260568 Number of successful extensions: 664 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 649 Number of HSP's gapped (non-prelim): 27 length of query: 151 length of database: 10,336,785 effective HSP length: 88 effective length of query: 63 effective length of database: 7,112,377 effective search space: 448079751 effective search space used: 448079751 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 54 (25.4 bits)