BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P60568 (153 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1PW6-A nrPDB 272 4e-74 5M5E-D nrPDB 211 1e-55 4YUE-C nrPDB 158 1e-39 4ZF7-A nrPDB 147 1e-36 3RTX-A nrPDB 28 1.9 5WV8-A nrPDB 26 7.9 >1PW6-A nrPDB Length = 133 Score = 272 bits (696), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 133/133 (100%), Positives = 133/133 (100%) Query: 21 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE 80 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE Sbjct: 1 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE 60 Query: 81 EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR 140 EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR Sbjct: 61 EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR 120 Query: 141 WITFCQSIISTLT 153 WITFCQSIISTLT Sbjct: 121 WITFCQSIISTLT 133 >5M5E-D nrPDB Length = 160 Score = 211 bits (537), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 135/149 (90%), Positives = 136/149 (91%), Gaps = 3/149 (2%) Query: 8 SCIALSL---ALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKF 64 SCI L L A +SAP SSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLT KF Sbjct: 4 SCIILFLVATATGVHSAPASSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTAKF 63 Query: 65 YMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFM 124 MPKKATELKHLQCLEEELKPLEEVLN AQSKNFHLRPRDLISNINVIVLELKGSETTFM Sbjct: 64 AMPKKATELKHLQCLEEELKPLEEVLNGAQSKNFHLRPRDLISNINVIVLELKGSETTFM 123 Query: 125 CEYADETATIVEFLNRWITFCQSIISTLT 153 CEYADETATIVEFLNRWITF QSIISTLT Sbjct: 124 CEYADETATIVEFLNRWITFAQSIISTLT 152 >4YUE-C nrPDB Length = 130 Score = 158 bits (399), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 76/120 (63%), Positives = 95/120 (79%), Gaps = 1/120 (0%) Query: 33 QLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNL 92 LE LL+DLQ +L+ + NY+N KL RMLTFKFY+PK+ATELK LQCLE+EL PL VL+L Sbjct: 5 HLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDL 64 Query: 93 AQSKNFHLR-PRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIIST 151 QSK+F L + ISNI V V++LKGS+ TF C++ DE+AT+V+FL RWI FCQSIIST Sbjct: 65 TQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIIST 124 >4ZF7-A nrPDB Length = 138 Score = 147 bits (372), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 101/135 (74%), Positives = 114/135 (84%), Gaps = 5/135 (3%) Query: 21 APT-SSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCL 79 APT SSSTK+ Q QLE LLLDLQ++LNG+ NY++P RMLTFKFYMPKKATEL HLQCL Sbjct: 1 APTTSSSTKEAQQQLEQLLLDLQLLLNGVKNYESP---RMLTFKFYMPKKATELTHLQCL 57 Query: 80 EEELKPLEEVLNLAQSKNFHLRP-RDLISNINVIVLELKGSETTFMCEYADETATIVEFL 138 EELK LEEVL LAQSKNFHL ++L+SNINV +L+LKGSET+F CEY DET TI EFL Sbjct: 58 AEELKLLEEVLYLAQSKNFHLTDIKELMSNINVTLLKLKGSETSFKCEYDDETVTITEFL 117 Query: 139 NRWITFCQSIISTLT 153 N+WITFCQSI STLT Sbjct: 118 NKWITFCQSIFSTLT 132 >3RTX-A nrPDB Length = 343 Score = 27.7 bits (60), Expect = 1.9, Method: Compositional matrix adjust. Identities = 26/99 (26%), Positives = 45/99 (45%), Gaps = 8/99 (8%) Query: 28 KKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKF-YMPKKATELK-HLQCLEEE-LK 84 K ++ L + LLD +MI++ +N P+ KF P + LQC+E E + Sbjct: 107 KDLRMHLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIIS 166 Query: 85 PLEEVLNLA----QSKNFHLRPRDLISNINVIVLELKGS 119 P E + + F +RP+ +IN+ L+G+ Sbjct: 167 PRHEKMKTGLIDKTQEPFSVRPKQFF-DINISRKLLEGN 204 >5WV8-A nrPDB Length = 482 Score = 25.8 bits (55), Expect = 7.9, Method: Composition-based stats. Identities = 13/27 (48%), Positives = 17/27 (62%) Query: 1 MYRMQLLSCIALSLALVTNSAPTSSST 27 M+ +LLSCI + +V APTS ST Sbjct: 1 MWPPRLLSCIFTIILIVAVIAPTSDST 27 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1PW6-A nrPDB 213 3e-56 5M5E-D nrPDB 193 2e-50 4YUE-C nrPDB 184 1e-47 4ZF7-A nrPDB 140 3e-34 Sequences not found previously or not previously below threshold: 3RTX-A nrPDB 30 0.49 4REP-A nrPDB 27 4.4 CONVERGED! >1PW6-A nrPDB Length = 133 Score = 213 bits (542), Expect = 3e-56, Method: Composition-based stats. Identities = 133/133 (100%), Positives = 133/133 (100%) Query: 21 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE 80 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE Sbjct: 1 APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE 60 Query: 81 EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR 140 EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR Sbjct: 61 EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR 120 Query: 141 WITFCQSIISTLT 153 WITFCQSIISTLT Sbjct: 121 WITFCQSIISTLT 133 >5M5E-D nrPDB Length = 160 Score = 193 bits (491), Expect = 2e-50, Method: Composition-based stats. Identities = 135/149 (90%), Positives = 136/149 (91%), Gaps = 3/149 (2%) Query: 8 SCIALSL---ALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKF 64 SCI L L A +SAP SSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLT KF Sbjct: 4 SCIILFLVATATGVHSAPASSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTAKF 63 Query: 65 YMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFM 124 MPKKATELKHLQCLEEELKPLEEVLN AQSKNFHLRPRDLISNINVIVLELKGSETTFM Sbjct: 64 AMPKKATELKHLQCLEEELKPLEEVLNGAQSKNFHLRPRDLISNINVIVLELKGSETTFM 123 Query: 125 CEYADETATIVEFLNRWITFCQSIISTLT 153 CEYADETATIVEFLNRWITF QSIISTLT Sbjct: 124 CEYADETATIVEFLNRWITFAQSIISTLT 152 >4YUE-C nrPDB Length = 130 Score = 184 bits (467), Expect = 1e-47, Method: Composition-based stats. Identities = 76/120 (63%), Positives = 95/120 (79%), Gaps = 1/120 (0%) Query: 33 QLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNL 92 LE LL+DLQ +L+ + NY+N KL RMLTFKFY+PK+ATELK LQCLE+EL PL VL+L Sbjct: 5 HLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDL 64 Query: 93 AQSKNFHLRP-RDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIIST 151 QSK+F L + ISNI V V++LKGS+ TF C++ DE+AT+V+FL RWI FCQSIIST Sbjct: 65 TQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIIST 124 >4ZF7-A nrPDB Length = 138 Score = 140 bits (353), Expect = 3e-34, Method: Composition-based stats. Identities = 99/132 (75%), Positives = 112/132 (84%), Gaps = 4/132 (3%) Query: 23 TSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEE 82 TSSSTK+ Q QLE LLLDLQ++LNG+ NY++P RMLTFKFYMPKKATEL HLQCL EE Sbjct: 4 TSSSTKEAQQQLEQLLLDLQLLLNGVKNYESP---RMLTFKFYMPKKATELTHLQCLAEE 60 Query: 83 LKPLEEVLNLAQSKNFHLRP-RDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRW 141 LK LEEVL LAQSKNFHL ++L+SNINV +L+LKGSET+F CEY DET TI EFLN+W Sbjct: 61 LKLLEEVLYLAQSKNFHLTDIKELMSNINVTLLKLKGSETSFKCEYDDETVTITEFLNKW 120 Query: 142 ITFCQSIISTLT 153 ITFCQSI STLT Sbjct: 121 ITFCQSIFSTLT 132 >3RTX-A nrPDB Length = 343 Score = 29.6 bits (65), Expect = 0.49, Method: Composition-based stats. Identities = 25/100 (25%), Positives = 46/100 (46%), Gaps = 10/100 (10%) Query: 28 KKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKF-YMPKKATELK-HLQCLEEELKP 85 K ++ L + LLD +MI++ +N P+ KF P + LQC+E E+ Sbjct: 107 KDLRMHLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIIS 166 Query: 86 LEE------VLNLAQSKNFHLRPRDLISNINVIVLELKGS 119 +++ Q + F +RP+ +IN+ L+G+ Sbjct: 167 PRHEKMKTGLIDKTQ-EPFSVRPKQFF-DINISRKLLEGN 204 >4REP-A nrPDB Length = 495 Score = 26.5 bits (57), Expect = 4.4, Method: Composition-based stats. Identities = 31/126 (24%), Positives = 49/126 (38%), Gaps = 16/126 (12%) Query: 12 LSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINN-YKNPKLTRML--------TF 62 L L + A T S + + L L LN N+ +KNPKLT++ + Sbjct: 137 LFLEKSLHKATTYFSLDTVKAIVHAPFLGLNNTLNDENSKFKNPKLTQLFNRYATYNGSS 196 Query: 63 KFYMPKKATELKHLQCLEEELKP-------LEEVLNLAQSKNFHLRPRDLISNINVIVLE 115 + P T + HL+ P + + LAQ R R+ ++NI + Sbjct: 197 PYQTPGIMTMIPHLELGLGTYYPDGGMHRISQSLFELAQKVGVKFRFRESVTNITTSKNK 256 Query: 116 LKGSET 121 + G ET Sbjct: 257 VTGVET 262 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.137 0.380 Lambda K H 0.267 0.0419 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,386,184 Number of Sequences: 36641 Number of extensions: 191797 Number of successful extensions: 545 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 529 Number of HSP's gapped (non-prelim): 19 length of query: 153 length of database: 10,336,785 effective HSP length: 89 effective length of query: 64 effective length of database: 7,075,736 effective search space: 452847104 effective search space used: 452847104 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)