BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P54657 (213 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1YHP-A nrPDB 438 e-123 5Z6D-A nrPDB 69 1e-12 5Z6E-A nrPDB 45 2e-05 4B7O-A nrPDB 35 0.020 4AIP-A nrPDB 35 0.021 1T7N-A nrPDB 30 0.90 1S5O-A nrPDB 29 1.1 4G6V-A nrPDB 27 5.0 3WVZ-A nrPDB 27 5.2 5HCE-A nrPDB 27 7.2 >1YHP-A nrPDB Length = 212 Score = 438 bits (1126), Expect = e-123, Method: Compositional matrix adjust. Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 2 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEHNEID 61 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEHNEID Sbjct: 1 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEHNEID 60 Query: 62 TPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTITP 121 TPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTITP Sbjct: 61 TPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTITP 120 Query: 122 YQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSPTTGQ 181 YQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSPTTGQ Sbjct: 121 YQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSPTTGQ 180 Query: 182 VTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK 213 VTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK Sbjct: 181 VTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK 212 >5Z6D-A nrPDB Length = 215 Score = 69.3 bits (168), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 60/212 (28%), Positives = 101/212 (47%), Gaps = 19/212 (8%) Query: 2 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEH-NEI 60 SVD KV F+ KN G Y G+ V NDK+ S VG++ + W+H NE Sbjct: 4 SVDPTKVIFYQKKNFEGSGDTYAVGQDVSVPG--SLNDKYFSVAVGASAKVIAWQHYNET 61 Query: 61 DTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTIT 120 G ++E ++ D++ I GLS+F+V+ + A+ + Y + + Sbjct: 62 -----GHYREWT--TSQADISDIGGLSRFRVVDDDTR-AISFLFKDATGGADKQYSLKVD 113 Query: 121 PYQVDKVAC--KDGDDFVQLPI-PKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSP 177 V V DGD++ + I P+ PP + + + VR H+ Y+ GSVYF+++ Sbjct: 114 ARDVGTVMLYSNDGDEYGLVGIMPEGGPP---VTTAVYVRDEHSGV-YIAVGSVYFEWNK 169 Query: 178 TTGQVTVIKKDETFPKNMTVTQDDNTSFIFNL 209 G+V V+ ++E +PK + + +SF L Sbjct: 170 DNGEVDVV-ENEHWPKQLKSKRTGKSSFEVTL 200 >5Z6E-A nrPDB Length = 101 Score = 45.4 bits (106), Expect = 2e-05, Method: Compositional matrix adjust. Identities = 31/92 (33%), Positives = 47/92 (51%), Gaps = 10/92 (10%) Query: 2 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEH-NEI 60 SVD KV F+ KN G Y G+ V NDK+ S VG++ + W+H NE Sbjct: 4 SVDPTKVIFYQKKNFEGSGDTYAVGQDVSVPG--SLNDKYFSVAVGASAKVIAWQHYNET 61 Query: 61 DTPTPGKFQELAQGSTNNDLTSINGLSKFQVL 92 G ++E ++ D++ I GLS+F+V+ Sbjct: 62 -----GHYREWT--TSQADISDIGGLSRFRVV 86 >4B7O-A nrPDB Length = 745 Score = 35.4 bits (80), Expect = 0.020, Method: Compositional matrix adjust. Identities = 26/89 (29%), Positives = 40/89 (44%), Gaps = 3/89 (3%) Query: 47 GSNVRCNIWEHNEIDTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVN 106 GS +R NI + D T +EL + + D NG S+F L G Q +VD+K+ N Sbjct: 64 GSKIRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDN 123 Query: 107 KVNSTAGSY---EMTITPYQVDKVACKDG 132 + + Y + P V V+ + G Sbjct: 124 AYSDSQILYHQGRFIVDPALVKVVSVQKG 152 >4AIP-A nrPDB Length = 742 Score = 35.0 bits (79), Expect = 0.021, Method: Compositional matrix adjust. Identities = 21/60 (35%), Positives = 30/60 (50%) Query: 47 GSNVRCNIWEHNEIDTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVN 106 GS +R NI + D T +EL + + D NG S+F L G Q +VD+K+ N Sbjct: 64 GSKIRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDN 123 >1T7N-A nrPDB Length = 618 Score = 29.6 bits (65), Expect = 0.90, Method: Compositional matrix adjust. Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Query: 26 GETVRFNNGDKWNDKFMSCLVGSNVRCN-IWEHNEIDTP 63 G +FN+G++W DK + +V + C ++EH + P Sbjct: 303 GGGSKFNSGNRWFDKTLQFIVAEDGSCGMVYEHAAAEGP 341 >1S5O-A nrPDB Length = 616 Score = 29.3 bits (64), Expect = 1.1, Method: Compositional matrix adjust. Identities = 12/39 (30%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Query: 26 GETVRFNNGDKWNDKFMSCLVGSNVRCN-IWEHNEIDTP 63 G R N+G++W DK + +V + C ++EH + P Sbjct: 290 GGGSRLNSGNRWFDKTLQFIVAEDGSCGLVYEHAAAEGP 328 >4G6V-A nrPDB Length = 176 Score = 27.3 bits (59), Expect = 5.0, Method: Compositional matrix adjust. Identities = 10/19 (52%), Positives = 13/19 (68%) Query: 164 DYVVNGSVYFKYSPTTGQV 182 DY +NG V+ Y+P TG V Sbjct: 93 DYTINGQVFDNYAPATGNV 111 >3WVZ-A nrPDB Length = 201 Score = 27.3 bits (59), Expect = 5.2, Method: Compositional matrix adjust. Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Query: 146 PDSEIVSHLTVRQTHT-PYDYVVNGSVYFKYSPTTG 180 PD E ++H+ V T P+ + GSVYF Y + G Sbjct: 32 PDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNG 67 >5HCE-A nrPDB Length = 998 Score = 26.9 bits (58), Expect = 7.2, Method: Compositional matrix adjust. Identities = 11/44 (25%), Positives = 25/44 (56%) Query: 75 STNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMT 118 ST + + +I GL+++ +L + ++D+ + K +Y+MT Sbjct: 608 STQDTINAIEGLTEYSLLVKQLRLSMDIDVSYKHKGALHNYKMT 651 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1YHP-A nrPDB 351 1e-97 5Z6D-A nrPDB 278 2e-75 5Z6E-A nrPDB 142 8e-35 Sequences not found previously or not previously below threshold: 4B7O-A nrPDB 33 0.12 4AIP-A nrPDB 32 0.14 3ENU-A nrPDB 30 0.91 1N5W-B nrPDB 28 3.1 1FOH-A nrPDB 27 5.9 CONVERGED! >1YHP-A nrPDB Length = 212 Score = 351 bits (901), Expect = 1e-97, Method: Composition-based stats. Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 2 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEHNEID 61 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEHNEID Sbjct: 1 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEHNEID 60 Query: 62 TPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTITP 121 TPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTITP Sbjct: 61 TPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTITP 120 Query: 122 YQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSPTTGQ 181 YQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSPTTGQ Sbjct: 121 YQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSPTTGQ 180 Query: 182 VTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK 213 VTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK Sbjct: 181 VTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK 212 >5Z6D-A nrPDB Length = 215 Score = 278 bits (710), Expect = 2e-75, Method: Composition-based stats. Identities = 61/216 (28%), Positives = 102/216 (47%), Gaps = 19/216 (8%) Query: 2 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEH-NEI 60 SVD KV F+ KN G Y G+ V NDK+ S VG++ + W+H NE Sbjct: 4 SVDPTKVIFYQKKNFEGSGDTYAVGQDVSVPG--SLNDKYFSVAVGASAKVIAWQHYNET 61 Query: 61 DTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTAGSYEMTIT 120 G ++E ++ D++ I GLS+F+V+ + A+ + Y + + Sbjct: 62 -----GHYREWT--TSQADISDIGGLSRFRVVDDDTR-AISFLFKDATGGADKQYSLKVD 113 Query: 121 PYQVDKVAC--KDGDDFVQLPI-PKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVYFKYSP 177 V V DGD++ + I P+ PP + + + VR H+ Y+ GSVYF+++ Sbjct: 114 ARDVGTVMLYSNDGDEYGLVGIMPEGGPP---VTTAVYVRDEHSGV-YIAVGSVYFEWNK 169 Query: 178 TTGQVTVIKKDETFPKNMTVTQDDNTSFIFNLNSEK 213 G+V V+ ++E +PK + + +SF L K Sbjct: 170 DNGEVDVV-ENEHWPKQLKSKRTGKSSFEVTLVDNK 204 >5Z6E-A nrPDB Length = 101 Score = 142 bits (359), Expect = 8e-35, Method: Composition-based stats. Identities = 32/108 (29%), Positives = 51/108 (47%), Gaps = 11/108 (10%) Query: 2 SVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCNIWEH-NEI 60 SVD KV F+ KN G Y G+ V NDK+ S VG++ + W+H NE Sbjct: 4 SVDPTKVIFYQKKNFEGSGDTYAVGQDVSVPG--SLNDKYFSVAVGASAKVIAWQHYNET 61 Query: 61 DTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKV 108 G ++E ++ D++ I GLS+F+V+ + A+ + Sbjct: 62 -----GHYREWT--TSQADISDIGGLSRFRVVDDDTR-AISFLFKDAT 101 >4B7O-A nrPDB Length = 745 Score = 32.8 bits (73), Expect = 0.12, Method: Composition-based stats. Identities = 27/97 (27%), Positives = 41/97 (42%), Gaps = 3/97 (3%) Query: 47 GSNVRCNIWEHNEIDTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVN 106 GS +R NI + D T +EL + + D NG S+F L G Q +VD+K+ N Sbjct: 64 GSKIRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDN 123 Query: 107 KVNSTAGSY---EMTITPYQVDKVACKDGDDFVQLPI 140 + + Y + P V V+ + G I Sbjct: 124 AYSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGI 160 >4AIP-A nrPDB Length = 742 Score = 32.4 bits (72), Expect = 0.14, Method: Composition-based stats. Identities = 27/97 (27%), Positives = 41/97 (42%), Gaps = 3/97 (3%) Query: 47 GSNVRCNIWEHNEIDTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVN 106 GS +R NI + D T +EL + + D NG S+F L G Q +VD+K+ N Sbjct: 64 GSKIRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDN 123 Query: 107 KVNSTAGSY---EMTITPYQVDKVACKDGDDFVQLPI 140 + + Y + P V V+ + G I Sbjct: 124 AYSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGI 160 >3ENU-A nrPDB Length = 114 Score = 29.7 bits (65), Expect = 0.91, Method: Composition-based stats. Identities = 19/58 (32%), Positives = 25/58 (43%), Gaps = 4/58 (6%) Query: 8 VKFFFGKNCTGESFEYNKGETVRF---NNGDKWNDKFMSCLVGSNVRCNIWE-HNEID 61 VKFF KN G+S + T+ G W +K S VG I++ HN D Sbjct: 25 VKFFDKKNFQGDSLFLSGPATLPRLIGPFGYDWENKVRSVKVGPRANLTIFDNHNYRD 82 >1N5W-B nrPDB Length = 809 Score = 27.8 bits (60), Expect = 3.1, Method: Composition-based stats. Identities = 13/50 (26%), Positives = 23/50 (46%), Gaps = 1/50 (2%) Query: 19 ESFEYNKGETVRFN-NGDKWNDKFMSCLVGSNVRCNIWEHNEIDTPTPGK 67 E+F G+ +R++ G+ FM + + V WE + TP+P Sbjct: 705 EAFAVAMGQEIRYDEQGNVLGASFMDFFLPTAVETPKWETDYTVTPSPHH 754 >1FOH-A nrPDB Length = 664 Score = 27.0 bits (58), Expect = 5.9, Method: Composition-based stats. Identities = 35/198 (17%), Positives = 63/198 (31%), Gaps = 22/198 (11%) Query: 1 MSVDANKVKFFFGKNCTGESFEYNKGETVRFNNGDKWNDKFMSCLVGSNVRCN------- 53 +S+D K F G N E + + + +C+VG+ + Sbjct: 436 VSMDVFKEAFVKGNEF-ASGTAINYDENLVTDKKSSKQELAKNCVVGTRFKSQPVVRHSE 494 Query: 54 -IWEHNEIDTPTPGKFQELAQGSTNNDLTSINGLSKFQVLPGAFQWAVDVKIVNKVNSTA 112 +W H T G+F+ + D T ++ + KF + + + + + Sbjct: 495 GLWMHFGDRLVTDGRFRIIVFAGKATDATQMSRIKKFSAYLDSENSVISLYTPKVSDRNS 554 Query: 113 GSYEMTITPYQVDKVACKDGDDFVQLPIPKLTPPDSEIVSHLTVRQTHTPYDYVVNGSVY 172 +TI D + D P P L P + H P+ Y Sbjct: 555 RIDVITIHSCHRDDIEMHD------FPAPALHPKWQYDFIYADCDSWHHPHP-----KSY 603 Query: 173 FKY--SPTTGQVTVIKKD 188 + T G V V++ D Sbjct: 604 QAWGVDETKGAVVVVRPD 621 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.305 0.125 0.331 Lambda K H 0.267 0.0372 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,812,639 Number of Sequences: 36641 Number of extensions: 304271 Number of successful extensions: 811 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 788 Number of HSP's gapped (non-prelim): 20 length of query: 213 length of database: 10,336,785 effective HSP length: 93 effective length of query: 120 effective length of database: 6,929,172 effective search space: 831500640 effective search space used: 831500640 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.0 bits) S2: 56 (26.3 bits)