BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P53725 (186 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5OKZ-J nrPDB 365 e-102 4ZPS-A nrPDB 36 0.010 5SZM-A nrPDB 35 0.014 5EPD-A nrPDB 28 1.6 2Q3R-A nrPDB 28 1.6 1ICQ-A nrPDB 27 5.0 2ZCU-A nrPDB 26 8.5 >5OKZ-J nrPDB Length = 190 Score = 365 bits (936), Expect = e-102, Method: Compositional matrix adjust. Identities = 186/186 (100%), Positives = 186/186 (100%) Query: 1 MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSA 60 MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSA Sbjct: 5 MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSA 64 Query: 61 WDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEGVISGRKTFGDNSD 120 WDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEGVISGRKTFGDNSD Sbjct: 65 WDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEGVISGRKTFGDNSD 124 Query: 121 DSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK 180 DSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK Sbjct: 125 DSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK 184 Query: 181 NRNSKK 186 NRNSKK Sbjct: 185 NRNSKK 190 >4ZPS-A nrPDB Length = 321 Score = 35.8 bits (81), Expect = 0.010, Method: Compositional matrix adjust. Identities = 16/45 (35%), Positives = 27/45 (60%), Gaps = 1/45 (2%) Query: 20 FMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSAWDLN 64 + G+ D EE +TP +N ++ +E+KGKLFG++ D+N Sbjct: 55 LVTAGRIDREELCAQSTPCLVNINIL-VEEKGKLFGVEIEITDIN 98 >5SZM-A nrPDB Length = 426 Score = 35.4 bits (80), Expect = 0.014, Method: Compositional matrix adjust. Identities = 16/45 (35%), Positives = 27/45 (60%), Gaps = 1/45 (2%) Query: 20 FMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSAWDLN 64 + G+ D EE +TP +N ++ +E+KGKLFG++ D+N Sbjct: 55 LVTAGRIDREELCAQSTPCLVNINIL-VEEKGKLFGVEIEITDIN 98 >5EPD-A nrPDB Length = 385 Score = 28.5 bits (62), Expect = 1.6, Method: Compositional matrix adjust. Identities = 39/154 (25%), Positives = 65/154 (42%), Gaps = 13/154 (8%) Query: 40 INSDVEPIEQKGKLFGLDDSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNV-- 97 I S+ I Q+G+ + D + D KK++ KK R+V + IS+V Sbjct: 74 IISEATQISQQGQGY-QDTPGIYTQAQIDGWKKVTAAVHKKGGRIVLQLWHVGRISHVNL 132 Query: 98 ---GYSELRKPEGVISGRKTFGDNS--DDSGSRKRKFDEGEQNEDEKRDAK----DKEFT 148 G + + P + + KTF +N D S R + +E D+ R A + F Sbjct: 133 QPNGGAPV-APSAIRAEVKTFVNNGFVDVSEPRALELEELAGIVDDFRKAAANSIEAGFD 191 Query: 149 GSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNKNR 182 G + G + Y L++ KD + + G +NR Sbjct: 192 GVEVHGANGYLLEQFAKDGANMRTDTYGGSVENR 225 >2Q3R-A nrPDB Length = 372 Score = 28.5 bits (62), Expect = 1.6, Method: Compositional matrix adjust. Identities = 12/44 (27%), Positives = 23/44 (52%) Query: 139 KRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNKNR 182 R+A + F G + G + Y +D+ KD++ + + G +NR Sbjct: 169 ARNAMEAGFDGVEIHGANGYLIDQFMKDTVNDRTDEYGGSLQNR 212 >1ICQ-A nrPDB Length = 376 Score = 26.9 bits (58), Expect = 5.0, Method: Compositional matrix adjust. Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 4/70 (5%) Query: 117 DNSDDSGSRKRKFDEGEQNEDE----KRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKK 172 D + + R+ DE Q +E R+A + F G + G Y +D+ KD + + Sbjct: 147 DIAHFTRPRRLTTDEIPQIVNEFRVAARNAIEAGFDGVEIHGAHGYLIDQFMKDQVNDRS 206 Query: 173 TNHNGKNKNR 182 + G +NR Sbjct: 207 DKYGGSLENR 216 >2ZCU-A nrPDB Length = 286 Score = 26.2 bits (56), Expect = 8.5, Method: Compositional matrix adjust. Identities = 24/83 (28%), Positives = 36/83 (43%), Gaps = 12/83 (14%) Query: 49 QKGKLFGLD-DSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEG 107 +GK++ L DSAW L +L K SGK+ V Y+ +S ++ K G Sbjct: 187 HEGKVYELAGDSAWTLTQLAAELTKQSGKQ------VTYQN-----LSEADFAAALKSVG 235 Query: 108 VISGRKTFGDNSDDSGSRKRKFD 130 + G +SD S+ FD Sbjct: 236 LPDGLADMLADSDVGASKGGLFD 258 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5OKZ-J nrPDB 279 4e-76 Sequences not found previously or not previously below threshold: 5SZM-A nrPDB 35 0.026 4ZPS-A nrPDB 35 0.026 2R0F-A nrPDB 31 0.24 2Q3R-A nrPDB 31 0.36 2ZCU-A nrPDB 30 0.58 1ICQ-A nrPDB 29 1.5 1DC1-A nrPDB 29 1.6 6GMH-V nrPDB 28 2.9 4HRG-A nrPDB 27 3.3 4FTG-A nrPDB 27 3.3 6EU0-Q nrPDB 27 4.5 1E9Y-A nrPDB 27 5.1 CONVERGED! >5OKZ-J nrPDB Length = 190 Score = 279 bits (714), Expect = 4e-76, Method: Composition-based stats. Identities = 186/186 (100%), Positives = 186/186 (100%) Query: 1 MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSA 60 MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSA Sbjct: 5 MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSA 64 Query: 61 WDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEGVISGRKTFGDNSD 120 WDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEGVISGRKTFGDNSD Sbjct: 65 WDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEGVISGRKTFGDNSD 124 Query: 121 DSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK 180 DSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK Sbjct: 125 DSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK 184 Query: 181 NRNSKK 186 NRNSKK Sbjct: 185 NRNSKK 190 >5SZM-A nrPDB Length = 426 Score = 34.7 bits (78), Expect = 0.026, Method: Composition-based stats. Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 1/41 (2%) Query: 24 GKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSAWDLN 64 G+ D EE +TP +N ++ +E+KGKLFG++ D+N Sbjct: 59 GRIDREELCAQSTPCLVNINIL-VEEKGKLFGVEIEITDIN 98 >4ZPS-A nrPDB Length = 321 Score = 34.7 bits (78), Expect = 0.026, Method: Composition-based stats. Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 1/41 (2%) Query: 24 GKTDDEESSNSNTPSNINSDVEPIEQKGKLFGLDDSAWDLN 64 G+ D EE +TP +N ++ +E+KGKLFG++ D+N Sbjct: 59 GRIDREELCAQSTPCLVNINIL-VEEKGKLFGVEIEITDIN 98 >2R0F-A nrPDB Length = 164 Score = 31.3 bits (69), Expect = 0.24, Method: Composition-based stats. Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 12/81 (14%) Query: 43 DVEPIEQKGKLFGLDDSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISN-VGYSE 101 D+EP G G+D++ +L + K D+ + + ++R N + N + Y E Sbjct: 31 DLEPSPNLGPT-GIDNTNVNLINAKGDV----------LLHIGIRRRENAFVFNSIPYGE 79 Query: 102 LRKPEGVISGRKTFGDNSDDS 122 R PE I TFGD D S Sbjct: 80 SRGPEERIPLEGTFGDRRDPS 100 >2Q3R-A nrPDB Length = 372 Score = 30.9 bits (68), Expect = 0.36, Method: Composition-based stats. Identities = 12/43 (27%), Positives = 23/43 (53%) Query: 140 RDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNKNR 182 R+A + F G + G + Y +D+ KD++ + + G +NR Sbjct: 170 RNAMEAGFDGVEIHGANGYLIDQFMKDTVNDRTDEYGGSLQNR 212 >2ZCU-A nrPDB Length = 286 Score = 30.1 bits (66), Expect = 0.58, Method: Composition-based stats. Identities = 24/86 (27%), Positives = 38/86 (44%), Gaps = 12/86 (13%) Query: 49 QKGKLFGL-DDSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKPEG 107 +GK++ L DSAW L +L K SGK+ V Y+ +S ++ K G Sbjct: 187 HEGKVYELAGDSAWTLTQLAAELTKQSGKQ------VTYQN-----LSEADFAAALKSVG 235 Query: 108 VISGRKTFGDNSDDSGSRKRKFDEGE 133 + G +SD S+ FD+ + Sbjct: 236 LPDGLADMLADSDVGASKGGLFDDSK 261 >1ICQ-A nrPDB Length = 376 Score = 28.6 bits (62), Expect = 1.5, Method: Composition-based stats. Identities = 17/62 (27%), Positives = 28/62 (45%), Gaps = 4/62 (6%) Query: 125 RKRKFDEGEQNEDE----KRDAKDKEFTGSQDDGEDEYDLDKLFKDSIKKKKTNHNGKNK 180 R+ DE Q +E R+A + F G + G Y +D+ KD + + + G + Sbjct: 155 RRLTTDEIPQIVNEFRVAARNAIEAGFDGVEIHGAHGYLIDQFMKDQVNDRSDKYGGSLE 214 Query: 181 NR 182 NR Sbjct: 215 NR 216 >1DC1-A nrPDB Length = 323 Score = 28.6 bits (62), Expect = 1.6, Method: Composition-based stats. Identities = 10/25 (40%), Positives = 17/25 (68%) Query: 141 DAKDKEFTGSQDDGEDEYDLDKLFK 165 D++DK++T D ED+Y+L+ K Sbjct: 161 DSRDKKYTNWMDKPEDDYELETFAK 185 >6GMH-V nrPDB Length = 613 Score = 27.8 bits (60), Expect = 2.9, Method: Composition-based stats. Identities = 19/48 (39%), Positives = 24/48 (50%), Gaps = 1/48 (2%) Query: 100 SELRKPEGVISGRKTFGDNSD-DSGSRKRKFDEGEQNEDEKRDAKDKE 146 SE E SG ++ + D D S K E E +EDE R A+DKE Sbjct: 486 SEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKE 533 >4HRG-A nrPDB Length = 115 Score = 27.4 bits (59), Expect = 3.3, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 22/37 (59%) Query: 130 DEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKD 166 D+G +++ R +KEF G ++ +D +DK+ KD Sbjct: 23 DKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKD 59 >4FTG-A nrPDB Length = 96 Score = 27.4 bits (59), Expect = 3.3, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 22/37 (59%) Query: 130 DEGEQNEDEKRDAKDKEFTGSQDDGEDEYDLDKLFKD 166 D+G +++ R +KEF G ++ +D +DK+ KD Sbjct: 21 DKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKD 57 >6EU0-Q nrPDB Length = 251 Score = 27.0 bits (58), Expect = 4.5, Method: Composition-based stats. Identities = 30/149 (20%), Positives = 61/149 (40%), Gaps = 30/149 (20%) Query: 4 NNGVTGKLSSRVMNMKFMKFGKT---------------DDEESSNS------------NT 36 N +T K R + +K++ FGKT D +E+S S +T Sbjct: 44 NGPITNK--ERSLAVKYINFGKTVKDGPFYTGSMSLIIDQQENSKSGKRKPNIILDEDDT 101 Query: 37 PSNINSDVEPIEQKGKL-FGLDDSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIIS 95 I + +K K+ +DD ++LN + ++L + G KKK+ + + + + Sbjct: 102 NDGIERYSDKYLKKRKIGISIDDHPYNLNLFPNELYNVMGINKKKLLAISKFNNADDVFT 161 Query: 96 NVGYSELRKPEGVISGRKTFGDNSDDSGS 124 G + +++ K ++ DD+ + Sbjct: 162 GTGLQDENIGLSMLAKLKELAEDVDDAST 190 >1E9Y-A nrPDB Length = 238 Score = 27.0 bits (58), Expect = 5.1, Method: Composition-based stats. Identities = 27/96 (28%), Positives = 39/96 (40%), Gaps = 6/96 (6%) Query: 46 PIEQKGKLFGLDDSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSELRKP 105 PIE GKL +L +D+ GK+ VK RP I S+ + E+ + Sbjct: 99 PIEANGKLV-----PGELFLKNEDITINEGKKAVSVKVKNVGDRPVQIGSHFHFFEVNRC 153 Query: 106 EGVISGRKTFGDNSDDSGSRKRKFDEGEQNEDEKRD 141 KTFG D + +F+ GE+ E D Sbjct: 154 LD-FDREKTFGKRLDIAAGTAVRFEPGEEKSVELID 188 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.304 0.127 0.345 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,769,200 Number of Sequences: 36641 Number of extensions: 344421 Number of successful extensions: 852 Number of sequences better than 10.0: 39 Number of HSP's better than 10.0 without gapping: 44 Number of HSP's successfully gapped in prelim test: 63 Number of HSP's that attempted gapping in prelim test: 672 Number of HSP's gapped (non-prelim): 220 length of query: 186 length of database: 10,336,785 effective HSP length: 91 effective length of query: 95 effective length of database: 7,002,454 effective search space: 665233130 effective search space used: 665233130 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.8 bits) S2: 56 (26.2 bits)