BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P53277 (215 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6J6G-I nrPDB 436 e-123 2QSZ-A nrPDB 27 6.7 >6J6G-I nrPDB Length = 215 Score = 436 bits (1121), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/215 (100%), Positives = 215/215 (100%) Query: 1 MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTE 60 MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTE Sbjct: 1 MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTE 60 Query: 61 SPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSS 120 SPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSS Sbjct: 61 SPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSS 120 Query: 121 KQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARK 180 KQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARK Sbjct: 121 KQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARK 180 Query: 181 RQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE 215 RQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE Sbjct: 181 RQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE 215 >2QSZ-A nrPDB Length = 207 Score = 26.9 bits (58), Expect = 6.7, Method: Compositional matrix adjust. Identities = 12/53 (22%), Positives = 26/53 (49%) Query: 83 PQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSSKQDSSADEEDNKNVP 135 P+ + + G + YD L L+ EK + ++ +++ S D+E + +P Sbjct: 59 PESEIETDKNGFLQYDVLEALNMEKMMSAISCWMESARHSVVSTDQESAEEIP 111 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6J6G-I nrPDB 342 4e-95 Sequences not found previously or not previously below threshold: 3ODM-A nrPDB 30 0.78 4U4P-B nrPDB 30 1.0 2QSZ-A nrPDB 29 1.2 CONVERGED! >6J6G-I nrPDB Length = 215 Score = 342 bits (878), Expect = 4e-95, Method: Composition-based stats. Identities = 215/215 (100%), Positives = 215/215 (100%) Query: 1 MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTE 60 MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTE Sbjct: 1 MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTE 60 Query: 61 SPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSS 120 SPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSS Sbjct: 61 SPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSS 120 Query: 121 KQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARK 180 KQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARK Sbjct: 121 KQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARK 180 Query: 181 RQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE 215 RQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE Sbjct: 181 RQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE 215 >3ODM-A nrPDB Length = 560 Score = 30.1 bits (66), Expect = 0.78, Method: Composition-based stats. Identities = 16/41 (39%), Positives = 23/41 (56%) Query: 17 RVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVG 57 R D ++ K A RE++E A KPR +S ED + E +G Sbjct: 297 RYDHGEEATKHAVRELKEKIAQSKPRNFSEEDKDLMKEFIG 337 >4U4P-B nrPDB Length = 247 Score = 29.8 bits (65), Expect = 1.0, Method: Composition-based stats. Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 9/87 (10%) Query: 98 DQLAKLSYEKTLRNLATQTQNSSKQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADD 157 DQ +++Y+K R Q + S V KGR+G + I Sbjct: 160 DQATRVAYQKDRRWRVVTLQGQIIEQSGTMTGGGSKV-MKGRMGS--------SLVIEIS 210 Query: 158 DKLVNKLAVSLQSESKKRYEARKRQMQ 184 ++ VNK+ LQ++SKK + +++++Q Sbjct: 211 EEEVNKMESQLQNDSKKAMQIQEQKVQ 237 >2QSZ-A nrPDB Length = 207 Score = 29.4 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 12/53 (22%), Positives = 26/53 (49%) Query: 83 PQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSSKQDSSADEEDNKNVP 135 P+ + + G + YD L L+ EK + ++ +++ S D+E + +P Sbjct: 59 PESEIETDKNGFLQYDVLEALNMEKMMSAISCWMESARHSVVSTDQESAEEIP 111 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.305 0.123 0.325 Lambda K H 0.267 0.0387 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,692,822 Number of Sequences: 36641 Number of extensions: 286142 Number of successful extensions: 612 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 22 Number of HSP's that attempted gapping in prelim test: 587 Number of HSP's gapped (non-prelim): 47 length of query: 215 length of database: 10,336,785 effective HSP length: 93 effective length of query: 122 effective length of database: 6,929,172 effective search space: 845358984 effective search space used: 845358984 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (22.0 bits) S2: 57 (26.6 bits)