BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P53218 (158 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AGB-F nrPDB 320 1e-88 4E8B-A nrPDB 31 0.19 3G4N-A nrPDB 28 1.6 5XF8-3 nrPDB 28 1.6 3LJS-A nrPDB 28 2.4 2Q2A-A nrPDB 27 2.8 1P4P-A nrPDB 27 4.7 5SWC-A nrPDB 27 4.8 1NXZ-A nrPDB 26 7.1 >6AGB-F nrPDB Length = 158 Score = 320 bits (821), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 158/158 (100%), Positives = 158/158 (100%) Query: 1 MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVN 60 MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVN Sbjct: 1 MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVN 60 Query: 61 KLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDI 120 KLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDI Sbjct: 61 KLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDI 120 Query: 121 KREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ 158 KREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ Sbjct: 121 KREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ 158 >4E8B-A nrPDB Length = 251 Score = 31.2 bits (69), Expect = 0.19, Method: Compositional matrix adjust. Identities = 16/49 (32%), Positives = 24/49 (48%) Query: 106 NKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLHS 154 NKK+ QW K+ ++ GRN + E R + + E + LNLH Sbjct: 125 NKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHP 173 >3G4N-A nrPDB Length = 470 Score = 28.1 bits (61), Expect = 1.6, Method: Compositional matrix adjust. Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 6/65 (9%) Query: 82 FSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDIKREGRNELQEERLKV--PILV 139 F GP+ K SI + K YI K + WN + I++ G + +Q +V P+ Sbjct: 343 FVIGPYKDKASSIRYQWDKRYIPGEVKWWDWN----WTIQQNGLSTMQNNLARVLRPVRA 398 Query: 140 TLVSD 144 + D Sbjct: 399 GITGD 403 >5XF8-3 nrPDB Length = 997 Score = 28.1 bits (61), Expect = 1.6, Method: Composition-based stats. Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 2/54 (3%) Query: 7 YN--EISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKS 58 YN EI + + I + +++ KE VIP L N Y + +DN KKS Sbjct: 668 YNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKS 721 >3LJS-A nrPDB Length = 338 Score = 27.7 bits (60), Expect = 2.4, Method: Compositional matrix adjust. Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Query: 32 PSLANNGNNSTSIQYHGISKNDNIKKSVNKLDKQIN--MADRSLGLQQV 78 P L NG N S + G+S D +K S +LD N AD + +QQ+ Sbjct: 171 PMLWPNGENPASRLWKGLSLADVVKLSSEELDYLANTLAADANAVIQQL 219 >2Q2A-A nrPDB Length = 272 Score = 27.3 bits (59), Expect = 2.8, Method: Compositional matrix adjust. Identities = 17/75 (22%), Positives = 35/75 (46%), Gaps = 17/75 (22%) Query: 49 ISKNDNIKKSVNKLDKQINMADRSLGLQQVVCIFSYGPHIQK----MLSILEIFKKG--- 101 + + +K +++ K I + + + G + +F GPHI+K +++I+E+ G Sbjct: 133 VKQGSPVKNALDLKGKTIGVQNATTGQEAAEKLFGKGPHIKKFETTVVAIMELLNGGVDA 192 Query: 102 ----------YIKNN 106 Y+KNN Sbjct: 193 VITDNAVANEYVKNN 207 >1P4P-A nrPDB Length = 145 Score = 26.6 bits (57), Expect = 4.7, Method: Compositional matrix adjust. Identities = 25/87 (28%), Positives = 38/87 (43%), Gaps = 13/87 (14%) Query: 39 NNSTSIQYHGISKNDNIKKSVNKLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIF 98 +N T+++Y I+ DN K+V L I + + SL + + G K EI Sbjct: 12 SNGTTLEYSQITDADNATKAVETLKNSIKL-EGSLVVGKTTVEIKEGTVTLKR----EIE 66 Query: 99 KKGYIK--------NNKKIYQWNKLTS 117 K G +K +NKK +W TS Sbjct: 67 KDGKVKVFLNDTAGSNKKTGKWEDSTS 93 >5SWC-A nrPDB Length = 239 Score = 26.6 bits (57), Expect = 4.8, Method: Compositional matrix adjust. Identities = 7/27 (25%), Positives = 20/27 (74%) Query: 85 GPHIQKMLSILEIFKKGYIKNNKKIYQ 111 G H+Q+++ L+ F++GY +++ +++ Sbjct: 17 GSHMQRLIEGLQKFREGYFSSHRDLFE 43 >1NXZ-A nrPDB Length = 246 Score = 26.2 bits (56), Expect = 7.1, Method: Compositional matrix adjust. Identities = 15/50 (30%), Positives = 25/50 (50%) Query: 104 KNNKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLH 153 + +KKI QW K+ ++ GRN + E R + + + + LNLH Sbjct: 122 RMDKKIQQWQKIAIAACEQCGRNIVPEIRPLMKLQDWCAENDGALKLNLH 171 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AGB-F nrPDB 311 5e-86 Sequences not found previously or not previously below threshold: 4E8B-A nrPDB 31 0.22 5XF8-3 nrPDB 29 0.88 2Q2A-A nrPDB 28 2.0 5SWC-A nrPDB 27 3.7 3LJS-A nrPDB 27 3.8 3VX8-D nrPDB 27 5.3 1NXZ-A nrPDB 26 6.3 CONVERGED! >6AGB-F nrPDB Length = 158 Score = 311 bits (798), Expect = 5e-86, Method: Composition-based stats. Identities = 158/158 (100%), Positives = 158/158 (100%) Query: 1 MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVN 60 MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVN Sbjct: 1 MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVN 60 Query: 61 KLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDI 120 KLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDI Sbjct: 61 KLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDI 120 Query: 121 KREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ 158 KREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ Sbjct: 121 KREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ 158 >4E8B-A nrPDB Length = 251 Score = 31.2 bits (69), Expect = 0.22, Method: Composition-based stats. Identities = 16/48 (33%), Positives = 24/48 (50%) Query: 106 NKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLH 153 NKK+ QW K+ ++ GRN + E R + + E + LNLH Sbjct: 125 NKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLH 172 >5XF8-3 nrPDB Length = 997 Score = 28.9 bits (63), Expect = 0.88, Method: Composition-based stats. Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 2/54 (3%) Query: 7 YN--EISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKS 58 YN EI + + I + +++ KE VIP L N Y + +DN KKS Sbjct: 668 YNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKS 721 >2Q2A-A nrPDB Length = 272 Score = 27.7 bits (60), Expect = 2.0, Method: Composition-based stats. Identities = 17/75 (22%), Positives = 35/75 (46%), Gaps = 17/75 (22%) Query: 49 ISKNDNIKKSVNKLDKQINMADRSLGLQQVVCIFSYGPHIQK----MLSILEIFKKG--- 101 + + +K +++ K I + + + G + +F GPHI+K +++I+E+ G Sbjct: 133 VKQGSPVKNALDLKGKTIGVQNATTGQEAAEKLFGKGPHIKKFETTVVAIMELLNGGVDA 192 Query: 102 ----------YIKNN 106 Y+KNN Sbjct: 193 VITDNAVANEYVKNN 207 >5SWC-A nrPDB Length = 239 Score = 27.0 bits (58), Expect = 3.7, Method: Composition-based stats. Identities = 7/27 (25%), Positives = 20/27 (74%) Query: 85 GPHIQKMLSILEIFKKGYIKNNKKIYQ 111 G H+Q+++ L+ F++GY +++ +++ Sbjct: 17 GSHMQRLIEGLQKFREGYFSSHRDLFE 43 >3LJS-A nrPDB Length = 338 Score = 27.0 bits (58), Expect = 3.8, Method: Composition-based stats. Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Query: 32 PSLANNGNNSTSIQYHGISKNDNIKKSVNKLDKQINM--ADRSLGLQQV 78 P L NG N S + G+S D +K S +LD N AD + +QQ+ Sbjct: 171 PMLWPNGENPASRLWKGLSLADVVKLSSEELDYLANTLAADANAVIQQL 219 >3VX8-D nrPDB Length = 323 Score = 26.6 bits (57), Expect = 5.3, Method: Composition-based stats. Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 8/68 (11%) Query: 2 INGVY----YNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKK 57 I G Y + ++S L + S + L ++++I S ++ N + GI N N + Sbjct: 43 ITGFYGPCGHPQVSNHLTLLSES--LPLDEQSLIASTSHGNRNKCPVP--GILYNTNTVE 98 Query: 58 SVNKLDKQ 65 S NKLDKQ Sbjct: 99 SFNKLDKQ 106 >1NXZ-A nrPDB Length = 246 Score = 26.2 bits (56), Expect = 6.3, Method: Composition-based stats. Identities = 15/48 (31%), Positives = 24/48 (50%) Query: 106 NKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLH 153 +KKI QW K+ ++ GRN + E R + + + + LNLH Sbjct: 124 DKKIQQWQKIAIAACEQCGRNIVPEIRPLMKLQDWCAENDGALKLNLH 171 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.134 0.372 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,734,858 Number of Sequences: 36641 Number of extensions: 203634 Number of successful extensions: 551 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 540 Number of HSP's gapped (non-prelim): 23 length of query: 158 length of database: 10,336,785 effective HSP length: 89 effective length of query: 69 effective length of database: 7,075,736 effective search space: 488225784 effective search space used: 488225784 T: 11 A: 40 X1: 15 ( 6.9 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)