BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P53178 (202 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2JZC-A nrPDB 417 e-117 2KS6-A nrPDB 416 e-117 1Q14-A nrPDB 31 0.36 5DU2-A nrPDB 31 0.46 1FSL-A nrPDB 29 1.1 3ENK-A nrPDB 27 5.7 6H7W-J nrPDB 27 7.0 2Q98-A nrPDB 27 7.5 4YNN-A nrPDB 27 7.8 3HHG-A nrPDB 27 8.6 1O9R-A nrPDB 27 8.6 >2JZC-A nrPDB Length = 224 Score = 417 bits (1072), Expect = e-117, Method: Compositional matrix adjust. Identities = 201/201 (100%), Positives = 201/201 (100%) Query: 2 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 61 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE Sbjct: 24 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 83 Query: 62 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 121 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG Sbjct: 84 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 143 Query: 122 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 181 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL Sbjct: 144 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 203 Query: 182 KPFPVSHNPSFERLLVETIYS 202 KPFPVSHNPSFERLLVETIYS Sbjct: 204 KPFPVSHNPSFERLLVETIYS 224 >2KS6-A nrPDB Length = 201 Score = 416 bits (1069), Expect = e-117, Method: Compositional matrix adjust. Identities = 201/201 (100%), Positives = 201/201 (100%) Query: 2 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 61 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE Sbjct: 1 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 60 Query: 62 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 121 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG Sbjct: 61 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 120 Query: 122 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 181 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL Sbjct: 121 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 180 Query: 182 KPFPVSHNPSFERLLVETIYS 202 KPFPVSHNPSFERLLVETIYS Sbjct: 181 KPFPVSHNPSFERLLVETIYS 201 >1Q14-A nrPDB Length = 361 Score = 30.8 bits (68), Expect = 0.36, Method: Compositional matrix adjust. Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Query: 51 YSSEF-EHLVQERGGQRESQKIPIDQFGCGDTARQYVL 87 YS EF E LV+E G Q + +KI Q G GD +++ +L Sbjct: 273 YSDEFAEQLVEELGWQEDFEKILTAQGGMGDNSKEQLL 310 >5DU2-A nrPDB Length = 419 Score = 30.8 bits (68), Expect = 0.46, Method: Compositional matrix adjust. Identities = 8/28 (28%), Positives = 21/28 (75%) Query: 109 DYSDLVISHAGTGSILDSLRLNKPLIVC 136 +++ L+++H G G+++D+L +P++V Sbjct: 315 EHARLLVTHGGIGAVMDALTAGRPMVVV 342 >1FSL-A nrPDB Length = 143 Score = 29.3 bits (64), Expect = 1.1, Method: Compositional matrix adjust. Identities = 19/72 (26%), Positives = 33/72 (45%), Gaps = 7/72 (9%) Query: 102 KMQSIIRD------YSDLVISHAGTGSILDSLRLNKPLIVCVNDSLMDNHQQQIADKFV- 154 K+ +++RD S V++ A GS+ + P V V ++L+ + + DK+ Sbjct: 64 KLFALVRDSAGQLKASGTVVADAALGSVHAQKAVTDPQFVVVKEALLKTIKAAVGDKWSD 123 Query: 155 ELGYVWSCAPTE 166 EL W A E Sbjct: 124 ELSRAWEVAYDE 135 >3ENK-A nrPDB Length = 341 Score = 26.9 bits (58), Expect = 5.7, Method: Compositional matrix adjust. Identities = 22/69 (31%), Positives = 30/69 (43%), Gaps = 5/69 (7%) Query: 64 GQRESQKIPIDQFGCGDTARQYVLMNG-----KLKVIGFDFSTKMQSIIRDYSDLVISHA 118 G ES I D G + YV KL+V G D+ T + +RDY +V Sbjct: 186 GAHESGLIGEDPAGIPNNLMPYVAQVAVGKLEKLRVFGSDYPTPDGTGVRDYIHVVDLAR 245 Query: 119 GTGSILDSL 127 G + LD+L Sbjct: 246 GHIAALDAL 254 >6H7W-J nrPDB Length = 292 Score = 26.6 bits (57), Expect = 7.0, Method: Compositional matrix adjust. Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 4/96 (4%) Query: 47 FGRNYSSEFEHLVQERGGQRESQKIPIDQFGCGDTARQYVLMNG---KLK-VIGFDFSTK 102 F R EF LVQE E Q F + +QY NG KL+ + S + Sbjct: 72 FDRGNHYEFLSLVQELAAPGELQHPQTFDFNFKNVEKQYESYNGINVKLRYFVRVTVSRR 131 Query: 103 MQSIIRDYSDLVISHAGTGSILDSLRLNKPLIVCVN 138 M +IR+ V S+ + S++++ + C++ Sbjct: 132 MADVIREKDIWVYSYRIPPELNSSIKMDVGIEDCLH 167 >2Q98-A nrPDB Length = 191 Score = 26.6 bits (57), Expect = 7.5, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 22/39 (56%) Query: 30 EFCQELIQYGFVRLIIQFGRNYSSEFEHLVQERGGQRES 68 E Q++ Q F+ LI+ R+++ HLV E G +E+ Sbjct: 62 EQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEA 100 >4YNN-A nrPDB Length = 448 Score = 26.6 bits (57), Expect = 7.8, Method: Compositional matrix adjust. Identities = 32/144 (22%), Positives = 65/144 (45%), Gaps = 7/144 (4%) Query: 8 ALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVR--LIIQFGRNYSSEFEHLVQERGGQ 65 A+ G V + ++ + Q++I++G + L+ F ++ + E L Q G Sbjct: 219 AIISPYGGNVGIGFAIPINMAKDVAQQIIKFGSIHRGLMGIFVQHLTPE---LAQSMGYA 275 Query: 66 RESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTGSILD 125 + Q + Q A+ L +G + V D TK+ + + + + AG+ + + Sbjct: 276 EDFQGALVSQVNQNSPAQLAGLKSGDVIVQIND--TKITQATQVKTTISLLRAGSTAKIK 333 Query: 126 SLRLNKPLIVCVNDSLMDNHQQQI 149 LR NKPL + V + + H+Q++ Sbjct: 334 ILRDNKPLTLDVEVTDIKKHEQKL 357 >3HHG-A nrPDB Length = 306 Score = 26.6 bits (57), Expect = 8.6, Method: Compositional matrix adjust. Identities = 14/27 (51%), Positives = 19/27 (70%) Query: 118 AGTGSILDSLRLNKPLIVCVNDSLMDN 144 A +G IL SL L+ IVC++D L+DN Sbjct: 220 ASSGEILRSLCLSGCGIVCLSDFLVDN 246 >1O9R-A nrPDB Length = 162 Score = 26.6 bits (57), Expect = 8.6, Method: Compositional matrix adjust. Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 14/64 (21%) Query: 129 LNKPLIVCVNDSL------MDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKLK 182 L P + V++ L +DNH IA++ V+LG T L + S T KLK Sbjct: 43 LKGPQFIAVHELLDTFRTQLDNHGDTIAERVVQLG--------GTALGSLQAVSSTTKLK 94 Query: 183 PFPV 186 +P Sbjct: 95 AYPT 98 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2KS6-A nrPDB 425 e-120 2JZC-A nrPDB 425 e-120 Sequences not found previously or not previously below threshold: 1Q14-A nrPDB 32 0.20 5DU2-A nrPDB 30 0.83 4YNN-A nrPDB 28 2.8 2Q98-A nrPDB 27 5.0 3HHG-A nrPDB 27 5.0 1FSL-A nrPDB 27 5.2 1PI6-A nrPDB 27 6.3 1O9R-A nrPDB 27 7.6 1A47-A nrPDB 26 9.3 6H7W-J nrPDB 26 9.5 CONVERGED! >2KS6-A nrPDB Length = 201 Score = 425 bits (1093), Expect = e-120, Method: Composition-based stats. Identities = 201/201 (100%), Positives = 201/201 (100%) Query: 2 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 61 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE Sbjct: 1 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 60 Query: 62 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 121 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG Sbjct: 61 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 120 Query: 122 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 181 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL Sbjct: 121 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 180 Query: 182 KPFPVSHNPSFERLLVETIYS 202 KPFPVSHNPSFERLLVETIYS Sbjct: 181 KPFPVSHNPSFERLLVETIYS 201 >2JZC-A nrPDB Length = 224 Score = 425 bits (1093), Expect = e-120, Method: Composition-based stats. Identities = 201/201 (100%), Positives = 201/201 (100%) Query: 2 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 61 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE Sbjct: 24 GIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLIIQFGRNYSSEFEHLVQE 83 Query: 62 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 121 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG Sbjct: 84 RGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTG 143 Query: 122 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 181 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL Sbjct: 144 SILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKL 203 Query: 182 KPFPVSHNPSFERLLVETIYS 202 KPFPVSHNPSFERLLVETIYS Sbjct: 204 KPFPVSHNPSFERLLVETIYS 224 >1Q14-A nrPDB Length = 361 Score = 31.9 bits (71), Expect = 0.20, Method: Composition-based stats. Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Query: 51 YSSEF-EHLVQERGGQRESQKIPIDQFGCGDTARQYVL 87 YS EF E LV+E G Q + +KI Q G GD +++ +L Sbjct: 273 YSDEFAEQLVEELGWQEDFEKILTAQGGMGDNSKEQLL 310 >5DU2-A nrPDB Length = 419 Score = 29.6 bits (65), Expect = 0.83, Method: Composition-based stats. Identities = 8/29 (27%), Positives = 22/29 (75%) Query: 107 IRDYSDLVISHAGTGSILDSLRLNKPLIV 135 + +++ L+++H G G+++D+L +P++V Sbjct: 313 VLEHARLLVTHGGIGAVMDALTAGRPMVV 341 >4YNN-A nrPDB Length = 448 Score = 28.1 bits (61), Expect = 2.8, Method: Composition-based stats. Identities = 29/125 (23%), Positives = 60/125 (48%), Gaps = 7/125 (5%) Query: 27 LSDEFCQELIQYGFVR--LIIQFGRNYSSEFEHLVQERGGQRESQKIPIDQFGCGDTARQ 84 ++ + Q++I++G + L+ F ++ + E L Q G + Q + Q A+ Sbjct: 238 MAKDVAQQIIKFGSIHRGLMGIFVQHLTPE---LAQSMGYAEDFQGALVSQVNQNSPAQL 294 Query: 85 YVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTGSILDSLRLNKPLIVCVNDSLMDN 144 L +G + V D TK+ + + + + AG+ + + LR NKPL + V + + Sbjct: 295 AGLKSGDVIVQIND--TKITQATQVKTTISLLRAGSTAKIKILRDNKPLTLDVEVTDIKK 352 Query: 145 HQQQI 149 H+Q++ Sbjct: 353 HEQKL 357 >2Q98-A nrPDB Length = 191 Score = 27.3 bits (59), Expect = 5.0, Method: Composition-based stats. Identities = 13/39 (33%), Positives = 22/39 (56%) Query: 30 EFCQELIQYGFVRLIIQFGRNYSSEFEHLVQERGGQRES 68 E Q++ Q F+ LI+ R+++ HLV E G +E+ Sbjct: 62 EQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEA 100 >3HHG-A nrPDB Length = 306 Score = 27.3 bits (59), Expect = 5.0, Method: Composition-based stats. Identities = 14/27 (51%), Positives = 19/27 (70%) Query: 118 AGTGSILDSLRLNKPLIVCVNDSLMDN 144 A +G IL SL L+ IVC++D L+DN Sbjct: 220 ASSGEILRSLCLSGCGIVCLSDFLVDN 246 >1FSL-A nrPDB Length = 143 Score = 27.3 bits (59), Expect = 5.2, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 7/74 (9%) Query: 100 STKMQSIIRDY------SDLVISHAGTGSILDSLRLNKPLIVCVNDSLMDNHQQQIADKF 153 + K+ +++RD S V++ A GS+ + P V V ++L+ + + DK+ Sbjct: 62 AEKLFALVRDSAGQLKASGTVVADAALGSVHAQKAVTDPQFVVVKEALLKTIKAAVGDKW 121 Query: 154 V-ELGYVWSCAPTE 166 EL W A E Sbjct: 122 SDELSRAWEVAYDE 135 >1PI6-A nrPDB Length = 615 Score = 26.9 bits (58), Expect = 6.3, Method: Composition-based stats. Identities = 11/28 (39%), Positives = 19/28 (67%) Query: 105 SIIRDYSDLVISHAGTGSILDSLRLNKP 132 +++ + DL+I + TG I+ S+RLN P Sbjct: 421 AVLTNDDDLLILQSFTGDIIKSVRLNSP 448 >1O9R-A nrPDB Length = 162 Score = 26.5 bits (57), Expect = 7.6, Method: Composition-based stats. Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 14/63 (22%) Query: 129 LNKPLIVCVNDSL------MDNHQQQIADKFVELGYVWSCAPTETGLIAGLRASQTEKLK 182 L P + V++ L +DNH IA++ V+LG T L + S T KLK Sbjct: 43 LKGPQFIAVHELLDTFRTQLDNHGDTIAERVVQLG--------GTALGSLQAVSSTTKLK 94 Query: 183 PFP 185 +P Sbjct: 95 AYP 97 >1A47-A nrPDB Length = 683 Score = 26.1 bits (56), Expect = 9.3, Method: Composition-based stats. Identities = 25/92 (27%), Positives = 39/92 (42%), Gaps = 14/92 (15%) Query: 80 DTARQYVLMNGKLKVIGFDFSTKMQSIIRDYSDLVISHAGTGSILDSLRLNKPLIVCVND 139 D Y + ++ F FS K++ + RD +D + G S++ S + I ND Sbjct: 267 DVNNTYFANESGMSLLDFRFSQKVRQVFRDNTDTMY---GLDSMIQSTASDYNFI---ND 320 Query: 140 --SLMDNHQQQIADKFVELGYVWSCAPTETGL 169 + +DNH D+F G S P E L Sbjct: 321 MVTFIDNHDM---DRFYNGG---STRPVEQAL 346 >6H7W-J nrPDB Length = 292 Score = 26.1 bits (56), Expect = 9.5, Method: Composition-based stats. Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 4/96 (4%) Query: 47 FGRNYSSEFEHLVQERGGQRESQKIPIDQFGCGDTARQYVLMNG---KLK-VIGFDFSTK 102 F R EF LVQE E Q F + +QY NG KL+ + S + Sbjct: 72 FDRGNHYEFLSLVQELAAPGELQHPQTFDFNFKNVEKQYESYNGINVKLRYFVRVTVSRR 131 Query: 103 MQSIIRDYSDLVISHAGTGSILDSLRLNKPLIVCVN 138 M +IR+ V S+ + S++++ + C++ Sbjct: 132 MADVIREKDIWVYSYRIPPELNSSIKMDVGIEDCLH 167 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.139 0.412 Lambda K H 0.267 0.0442 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,332,251 Number of Sequences: 36641 Number of extensions: 416238 Number of successful extensions: 895 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 880 Number of HSP's gapped (non-prelim): 33 length of query: 202 length of database: 10,336,785 effective HSP length: 92 effective length of query: 110 effective length of database: 6,965,813 effective search space: 766239430 effective search space used: 766239430 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 57 (26.5 bits)