BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P52871
         (88 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2WW9-C nrPDB                                                          176   2e-45
6N3Q-B nrPDB                                                           91   1e-19
2WWB-C nrPDB                                                           41   1e-04
3ASN-B nrPDB                                                           30   0.32 
5NGW-A nrPDB                                                           26   4.6  
6AGI-A nrPDB                                                           25   5.4  
4G1E-B nrPDB                                                           25   6.1  
3T3P-B nrPDB                                                           25   6.1  
4MB6-A nrPDB                                                           25   7.6  

>2WW9-C nrPDB
          Length = 87

 Score =  176 bits (446), Expect = 2e-45,   Method: Compositional matrix adjust.
 Identities = 87/87 (100%), Positives = 87/87 (100%)

Query: 1  MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD 60
          MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD
Sbjct: 1  MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD 60

Query: 61 SLVVLFLSVGFIFSVIALHLLTKFTHI 87
          SLVVLFLSVGFIFSVIALHLLTKFTHI
Sbjct: 61 SLVVLFLSVGFIFSVIALHLLTKFTHI 87


>6N3Q-B nrPDB
          Length = 82

 Score = 90.9 bits (224), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 46/83 (55%), Positives = 59/83 (71%), Gaps = 7/83 (8%)

Query: 1  MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD 60
          M++  PPGGQR LQKR+Q  S  +K A   P          S++SILK+Y+DEA G RVD
Sbjct: 1  MSSPTPPGGQRTLQKRKQGSS--QKVAASAPKKNTN-----SNNSILKIYSDEATGLRVD 53

Query: 61 SLVVLFLSVGFIFSVIALHLLTK 83
           LVVLFL+VGFIFSV+ALH+++K
Sbjct: 54 PLVVLFLAVGFIFSVVALHVISK 76


>2WWB-C nrPDB
          Length = 96

 Score = 41.2 bits (95), Expect = 1e-04,   Method: Compositional matrix adjust.
 Identities = 22/64 (34%), Positives = 36/64 (56%), Gaps = 2/64 (3%)

Query: 25 KQAKQTPTSTRQAGYGGSSSS--ILKLYTDEANGFRVDSLVVLFLSVGFIFSVIALHLLT 82
          +Q K     TR AG   S+ +  + + YT+++ G +V  + VL +S+ FI SV  LH+  
Sbjct: 32 RQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWG 91

Query: 83 KFTH 86
          K+T 
Sbjct: 92 KYTR 95


>3ASN-B nrPDB
          Length = 227

 Score = 29.6 bits (65), Expect = 0.32,   Method: Compositional matrix adjust.
 Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 4/51 (7%)

Query: 36 QAGYGGSSSSILKLYTDEANGFRVDSLVVLFLSVGFIFSVIALHLLTKFTH 86
          Q G+  ++S I+    +E   F   +L+++FL    +  +I+L L TK TH
Sbjct: 6  QLGFQDATSPIM----EELLHFHDHTLMIVFLISSLVLYIISLMLTTKLTH 52


>5NGW-A nrPDB
          Length = 389

 Score = 25.8 bits (55), Expect = 4.6,   Method: Composition-based stats.
 Identities = 13/31 (41%), Positives = 18/31 (58%)

Query: 33  STRQAGYGGSSSSILKLYTDEANGFRVDSLV 63
           S++ AG   SS  I+  Y +E  GF  DS+V
Sbjct: 102 SSQNAGGHSSSFDIVARYNNELAGFTPDSIV 132


>6AGI-A nrPDB
          Length = 382

 Score = 25.4 bits (54), Expect = 5.4,   Method: Composition-based stats.
 Identities = 12/44 (27%), Positives = 23/44 (52%)

Query: 10  QRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDE 53
           Q I +K+ + + IK  + K+     +  GY   ++S+LK   +E
Sbjct: 131 QEIFRKKNEKREIKGDEEKRAMLRLQLYGYHSPTNSVLKTDAEE 174


>4G1E-B nrPDB
          Length = 738

 Score = 25.4 bits (54), Expect = 6.1,   Method: Composition-based stats.
 Identities = 15/54 (27%), Positives = 24/54 (44%)

Query: 8   GGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVDS 61
           G + +L    Q     E+  KQ+ +  R A  GG  + +     DE  G+R D+
Sbjct: 189 GYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDA 242


>3T3P-B nrPDB
          Length = 472

 Score = 25.4 bits (54), Expect = 6.1,   Method: Composition-based stats.
 Identities = 15/54 (27%), Positives = 24/54 (44%)

Query: 8   GGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVDS 61
           G + +L    Q     E+  KQ+ +  R A  GG  + +     DE  G+R D+
Sbjct: 189 GYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDA 242


>4MB6-A nrPDB
          Length = 187

 Score = 25.0 bits (53), Expect = 7.6,   Method: Compositional matrix adjust.
 Identities = 13/31 (41%), Positives = 17/31 (54%)

Query: 42 SSSSILKLYTDEANGFRVDSLVVLFLSVGFI 72
          S S + K+   EA GF   + V L L VGF+
Sbjct: 57 SESGVTKVVGTEARGFLFGAPVALALGVGFV 87


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

2WW9-C nrPDB                                                          134   1e-32
6N3Q-B nrPDB                                                          107   2e-24
2WWB-C nrPDB                                                           90   2e-19

Sequences not found previously or not previously below threshold:

3J92-1 nrPDB                                                           50   2e-07
3L0I-A nrPDB                                                           27   2.1  
2WWX-B nrPDB                                                           27   2.1  
3N6O-A nrPDB                                                           27   2.4  

>2WW9-C nrPDB
          Length = 87

 Score =  134 bits (336), Expect = 1e-32,   Method: Composition-based stats.
 Identities = 87/87 (100%), Positives = 87/87 (100%)

Query: 1  MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD 60
          MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD
Sbjct: 1  MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD 60

Query: 61 SLVVLFLSVGFIFSVIALHLLTKFTHI 87
          SLVVLFLSVGFIFSVIALHLLTKFTHI
Sbjct: 61 SLVVLFLSVGFIFSVIALHLLTKFTHI 87


>6N3Q-B nrPDB
          Length = 82

 Score =  107 bits (266), Expect = 2e-24,   Method: Composition-based stats.
 Identities = 46/83 (55%), Positives = 59/83 (71%), Gaps = 7/83 (8%)

Query: 1  MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTDEANGFRVD 60
          M++  PPGGQR LQKR+Q  S  +K A   P          S++SILK+Y+DEA G RVD
Sbjct: 1  MSSPTPPGGQRTLQKRKQGSS--QKVAASAPKKNTN-----SNNSILKIYSDEATGLRVD 53

Query: 61 SLVVLFLSVGFIFSVIALHLLTK 83
           LVVLFL+VGFIFSV+ALH+++K
Sbjct: 54 PLVVLFLAVGFIFSVVALHVISK 76


>2WWB-C nrPDB
          Length = 96

 Score = 90.2 bits (222), Expect = 2e-19,   Method: Composition-based stats.
 Identities = 26/97 (26%), Positives = 44/97 (45%), Gaps = 13/97 (13%)

Query: 1  MAASVPPGGQ---------RILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSS--ILKL 49
          M    P G           + +  R    ++  +Q K     TR AG   S+ +  + + 
Sbjct: 1  MPGPTPSGTNVGSSGRSPSKAVAARAAGSTV--RQRKNASCGTRSAGRTTSAGTGGMWRF 58

Query: 50 YTDEANGFRVDSLVVLFLSVGFIFSVIALHLLTKFTH 86
          YT+++ G +V  + VL +S+ FI SV  LH+  K+T 
Sbjct: 59 YTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTR 95


>3J92-1 nrPDB
          Length = 104

 Score = 50.1 bits (118), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 12/51 (23%), Positives = 25/51 (49%), Gaps = 4/51 (7%)

Query: 11  RILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSS--ILKLYTDEANGFRV 59
           + +  R    ++  +Q K     TR AG   S+ +  + + YT+++ G +V
Sbjct: 56  KAVAARAAGSTV--RQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKV 104


>3L0I-A nrPDB
          Length = 363

 Score = 27.0 bits (58), Expect = 2.1,   Method: Composition-based stats.
 Identities = 18/82 (21%), Positives = 29/82 (35%), Gaps = 10/82 (12%)

Query: 4   SVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTD----------E 53
           ++ P G  ILQ    A S   + A     ST + G   + + +   + D           
Sbjct: 210 TISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSPNERATP 269

Query: 54  ANGFRVDSLVVLFLSVGFIFSV 75
              F+V    +L   + FI  V
Sbjct: 270 DPDFKVGKSKILVGIMQFIKDV 291


>2WWX-B nrPDB
          Length = 217

 Score = 27.0 bits (58), Expect = 2.1,   Method: Composition-based stats.
 Identities = 18/82 (21%), Positives = 29/82 (35%), Gaps = 10/82 (12%)

Query: 4   SVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTD----------E 53
           ++ P G  ILQ    A S   + A     ST + G   + + +   + D           
Sbjct: 81  TISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSPNERATP 140

Query: 54  ANGFRVDSLVVLFLSVGFIFSV 75
              F+V    +L   + FI  V
Sbjct: 141 DPDFKVGKSKILVGIMQFIKDV 162


>3N6O-A nrPDB
          Length = 311

 Score = 26.6 bits (57), Expect = 2.4,   Method: Composition-based stats.
 Identities = 18/82 (21%), Positives = 29/82 (35%), Gaps = 10/82 (12%)

Query: 4   SVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSSSILKLYTD----------E 53
           ++ P G  ILQ    A S   + A     ST + G   + + +   + D           
Sbjct: 61  TISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSPNERATP 120

Query: 54  ANGFRVDSLVVLFLSVGFIFSV 75
              F+V    +L   + FI  V
Sbjct: 121 DPDFKVGKSKILVGIMQFIKDV 142


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.309    0.129    0.330 

Lambda     K      H
   0.267   0.0384    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,698,763
Number of Sequences: 36641
Number of extensions: 68753
Number of successful extensions: 175
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 156
Number of HSP's gapped (non-prelim): 17
length of query: 88
length of database: 10,336,785
effective HSP length: 57
effective length of query: 31
effective length of database: 8,248,248
effective search space: 255695688
effective search space used: 255695688
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.2 bits)
S2: 52 (24.7 bits)