BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P52657 (109 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5IYA-O nrPDB 223 1e-59 5M4S-A nrPDB 206 2e-54 6MZM-X nrPDB 197 6e-52 6GYK-V nrPDB 89 5e-19 5US9-A nrPDB 30 0.27 5CFD-B nrPDB 29 0.52 5US7-A nrPDB 29 0.56 5A8F-B nrPDB 28 0.74 6IOX-A nrPDB 27 2.5 3HSI-A nrPDB 26 3.3 3LJ5-A nrPDB 26 4.8 2O1S-A nrPDB 25 6.1 >5IYA-O nrPDB Length = 109 Score = 223 bits (568), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 109/109 (100%), Positives = 109/109 (100%) Query: 1 MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRG 60 MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRG Sbjct: 1 MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRG 60 Query: 61 SLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE 109 SLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE Sbjct: 61 SLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE 109 >5M4S-A nrPDB Length = 209 Score = 206 bits (525), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 101/106 (95%), Positives = 103/106 (97%) Query: 2 AYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGS 61 AYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGS Sbjct: 1 AYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGS 60 Query: 62 LNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNT 107 LNTYRFCDNVWTFVLNDVE REVTELIKVDKVKIVACDGKNT ++ Sbjct: 61 LNTYRFCDNVWTFVLNDVEVREVTELIKVDKVKIVACDGKNTANSA 106 >6MZM-X nrPDB Length = 97 Score = 197 bits (502), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 97/97 (100%), Positives = 97/97 (100%) Query: 3 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 62 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL Sbjct: 1 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 60 Query: 63 NTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACD 99 NTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACD Sbjct: 61 NTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACD 97 >6GYK-V nrPDB Length = 129 Score = 88.6 bits (218), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 43/115 (37%), Positives = 72/115 (62%), Gaps = 14/115 (12%) Query: 3 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 62 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Query: 63 NTYRFCDNVWTFVLNDVE------FREVTE--------LIKVDKVKIVACDGKNT 103 +TY FCD+VWTF++ + + R+ ++ +I VDK++IVAC+ K + Sbjct: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKS 121 >5US9-A nrPDB Length = 541 Score = 30.0 bits (66), Expect = 0.27, Method: Composition-based stats. Identities = 10/23 (43%), Positives = 15/23 (65%) Query: 52 VRNRVNFRGSLNTYRFCDNVWTF 74 +R++ FRG+ TY C +VW F Sbjct: 380 LRDQALFRGNQTTYNLCSDVWMF 402 >5CFD-B nrPDB Length = 232 Score = 28.9 bits (63), Expect = 0.52, Method: Compositional matrix adjust. Identities = 16/39 (41%), Positives = 19/39 (48%) Query: 25 SQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLN 63 S TP + QV L N+ LA RN +RGSLN Sbjct: 72 STPATPLVTYQVTLSCSCMANSMLAAVARNFNQYRGSLN 110 >5US7-A nrPDB Length = 539 Score = 28.9 bits (63), Expect = 0.56, Method: Composition-based stats. Identities = 9/23 (39%), Positives = 15/23 (65%) Query: 52 VRNRVNFRGSLNTYRFCDNVWTF 74 +R++ +RG+ TY C +VW F Sbjct: 378 LRDQTLYRGNQTTYNLCSDVWMF 400 >5A8F-B nrPDB Length = 189 Score = 28.5 bits (62), Expect = 0.74, Method: Compositional matrix adjust. Identities = 16/39 (41%), Positives = 19/39 (48%) Query: 25 SQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLN 63 S TP + QV L N+ LA RN +RGSLN Sbjct: 29 STPATPLVTYQVTLSCSCMANSMLAAVARNFNQYRGSLN 67 >6IOX-A nrPDB Length = 340 Score = 26.6 bits (57), Expect = 2.5, Method: Composition-based stats. Identities = 11/44 (25%), Positives = 28/44 (63%) Query: 9 TTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRV 52 +T G++ E D+++++ ++ ++A +L+ + +AAL +RV Sbjct: 216 STKGSAKHEMTDKVVEATRMAQEMAPDLLIDGEMQADAALVERV 259 >3HSI-A nrPDB Length = 458 Score = 26.2 bits (56), Expect = 3.3, Method: Compositional matrix adjust. Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 7/63 (11%) Query: 16 QESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFV 75 QE LDE+ + +Q P L ++VL+ + +A L + + N +C+ T+ Sbjct: 67 QEILDEIYRVKQENPHLDVKVLIDWHRAQRNLLGAE-------KSATNADWYCEQRQTYQ 119 Query: 76 LND 78 L D Sbjct: 120 LPD 122 >3LJ5-A nrPDB Length = 725 Score = 25.8 bits (55), Expect = 4.8, Method: Compositional matrix adjust. Identities = 12/28 (42%), Positives = 16/28 (57%) Query: 54 NRVNFRGSLNTYRFCDNVWTFVLNDVEF 81 N++N R L TY F DN+ T + D E Sbjct: 436 NQLNMRADLETYVFQDNLATAMRRDGEI 463 >2O1S-A nrPDB Length = 621 Score = 25.4 bits (54), Expect = 6.1, Method: Composition-based stats. Identities = 11/35 (31%), Positives = 19/35 (54%) Query: 67 FCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGK 101 F + VL+DV +++ L +D+ IV DG+ Sbjct: 395 FLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQ 429 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5IYA-O nrPDB 174 1e-44 5M4S-A nrPDB 172 4e-44 6MZM-X nrPDB 156 2e-39 6GYK-V nrPDB 141 5e-35 Sequences not found previously or not previously below threshold: 5US9-A nrPDB 31 0.13 5US7-A nrPDB 30 0.27 2W0A-A nrPDB 28 1.3 3HLD-A nrPDB 27 2.5 6QPR-A nrPDB 26 3.5 5URF-A nrPDB 26 5.0 3WFL-A nrPDB 25 6.3 3LJ5-A nrPDB 25 9.6 2XF8-A nrPDB 25 9.7 CONVERGED! >5IYA-O nrPDB Length = 109 Score = 174 bits (440), Expect = 1e-44, Method: Composition-based stats. Identities = 109/109 (100%), Positives = 109/109 (100%) Query: 1 MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRG 60 MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRG Sbjct: 1 MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRG 60 Query: 61 SLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE 109 SLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE Sbjct: 61 SLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE 109 >5M4S-A nrPDB Length = 209 Score = 172 bits (435), Expect = 4e-44, Method: Composition-based stats. Identities = 101/106 (95%), Positives = 103/106 (97%) Query: 2 AYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGS 61 AYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGS Sbjct: 1 AYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGS 60 Query: 62 LNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNT 107 LNTYRFCDNVWTFVLNDVE REVTELIKVDKVKIVACDGKNT ++ Sbjct: 61 LNTYRFCDNVWTFVLNDVEVREVTELIKVDKVKIVACDGKNTANSA 106 >6MZM-X nrPDB Length = 97 Score = 156 bits (395), Expect = 2e-39, Method: Composition-based stats. Identities = 97/97 (100%), Positives = 97/97 (100%) Query: 3 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 62 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL Sbjct: 1 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 60 Query: 63 NTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACD 99 NTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACD Sbjct: 61 NTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACD 97 >6GYK-V nrPDB Length = 129 Score = 141 bits (356), Expect = 5e-35, Method: Composition-based stats. Identities = 43/115 (37%), Positives = 72/115 (62%), Gaps = 14/115 (12%) Query: 3 YQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSL 62 Y+LYR +T+GNSL ++LD LI +I LA++VL FDK + L ++++ +G+L Sbjct: 7 YELYRRSTIGNSLVDALDTLISDGRIEASLAMRVLETFDKVVAETLKDNTQSKLTVKGNL 66 Query: 63 NTYRFCDNVWTFVLNDVE------FREVTE--------LIKVDKVKIVACDGKNT 103 +TY FCD+VWTF++ + + R+ ++ +I VDK++IVAC+ K + Sbjct: 67 DTYGFCDDVWTFIVKNCQVTVEDSHRDASQNGSGDSQSVISVDKLRIVACNSKKS 121 >5US9-A nrPDB Length = 541 Score = 30.8 bits (68), Expect = 0.13, Method: Composition-based stats. Identities = 10/23 (43%), Positives = 15/23 (65%) Query: 52 VRNRVNFRGSLNTYRFCDNVWTF 74 +R++ FRG+ TY C +VW F Sbjct: 380 LRDQALFRGNQTTYNLCSDVWMF 402 >5US7-A nrPDB Length = 539 Score = 30.0 bits (66), Expect = 0.27, Method: Composition-based stats. Identities = 9/23 (39%), Positives = 15/23 (65%) Query: 52 VRNRVNFRGSLNTYRFCDNVWTF 74 +R++ +RG+ TY C +VW F Sbjct: 378 LRDQTLYRGNQTTYNLCSDVWMF 400 >2W0A-A nrPDB Length = 455 Score = 27.7 bits (60), Expect = 1.3, Method: Composition-based stats. Identities = 10/46 (21%), Positives = 20/46 (43%) Query: 4 QLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALA 49 +L R+ ++ + LDEL + AL+ + Q + + L Sbjct: 271 ELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLR 316 >3HLD-A nrPDB Length = 432 Score = 26.5 bits (57), Expect = 2.5, Method: Composition-based stats. Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 4/42 (9%) Query: 19 LDELIQSQQITPQL-ALQVLLQFDKAINAALAQRVRNRVNFR 59 LDE + ++ P L A+ VL FD A NA L +R R ++ R Sbjct: 116 LDETVD--RLLPDLSAMPVLEGFDDAGNARLRER-RGKITLR 154 >6QPR-A nrPDB Length = 361 Score = 26.2 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 15/57 (26%), Positives = 26/57 (45%), Gaps = 1/57 (1%) Query: 36 VLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDK 92 V+ D I AA +Q + N + + +L+ +C V D + TE +K+ K Sbjct: 90 VVQAMDGGIRAATSQEI-NELTYYTTLSANSYCRTVIPGATWDCIHCDATEDLKIIK 145 >5URF-A nrPDB Length = 542 Score = 25.8 bits (55), Expect = 5.0, Method: Composition-based stats. Identities = 6/23 (26%), Positives = 15/23 (65%) Query: 52 VRNRVNFRGSLNTYRFCDNVWTF 74 +R+++ ++G+ TY ++W F Sbjct: 381 LRDQLLYKGNQTTYNLVGDIWMF 403 >3WFL-A nrPDB Length = 342 Score = 25.4 bits (54), Expect = 6.3, Method: Composition-based stats. Identities = 10/46 (21%), Positives = 20/46 (43%) Query: 21 ELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYR 66 +L+Q T L + D +++A V+ +NF + + Y Sbjct: 72 QLLQDGTATINTGSDGLERLDYVVSSAEQHNVKLIINFVNNWSDYG 117 >3LJ5-A nrPDB Length = 725 Score = 24.6 bits (52), Expect = 9.6, Method: Composition-based stats. Identities = 13/34 (38%), Positives = 18/34 (52%) Query: 48 LAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEF 81 +A N++N R L TY F DN+ T + D E Sbjct: 430 VAFDTVNQLNMRADLETYVFQDNLATAMRRDGEI 463 >2XF8-A nrPDB Length = 338 Score = 24.6 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 11/39 (28%), Positives = 20/39 (51%) Query: 36 VLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTF 74 V + F+ ++A+ + RV+ + T +CDN W F Sbjct: 283 VSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGF 321 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.137 0.363 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,725,318 Number of Sequences: 36641 Number of extensions: 118282 Number of successful extensions: 303 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 275 Number of HSP's gapped (non-prelim): 31 length of query: 109 length of database: 10,336,785 effective HSP length: 75 effective length of query: 34 effective length of database: 7,588,710 effective search space: 258016140 effective search space used: 258016140 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)