BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P50637 (169 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2MGY-A nrPDB 328 6e-91 4UC1-A nrPDB 72 1e-13 4RYM-A nrPDB 53 5e-08 4XGV-A nrPDB 28 2.2 2A5Y-A nrPDB 27 5.6 >2MGY-A nrPDB Length = 169 Score = 328 bits (842), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 167/169 (98%), Positives = 167/169 (98%) Query: 1 MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYASLQKPSWHPPRWTLAPIWGTLYSAM 60 MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYA LQKPSWHPPRWTLAPIWGTLYSAM Sbjct: 1 MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYAGLQKPSWHPPRWTLAPIWGTLYSAM 60 Query: 61 GYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVAT 120 GYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVAT Sbjct: 61 GYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVAT 120 Query: 121 ATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRLPE 169 ATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRL E Sbjct: 121 ATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRLAE 169 >4UC1-A nrPDB Length = 157 Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 47/130 (36%), Positives = 64/130 (49%), Gaps = 5/130 (3%) Query: 32 RWYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLAL 91 WY +L KP W+PPRW W +LY M + V +L G + L Y QLA Sbjct: 29 EWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRV-AQLEGSGQ----ALAFYAAQLAF 83 Query: 92 NWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLN 151 N W P+FFG ++M ALA ++++ AT A+ ++ A L PYL W T LN Sbjct: 84 NTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATATTGLN 143 Query: 152 YYVWRDNSGR 161 + R N R Sbjct: 144 FEAMRLNWNR 153 >4RYM-A nrPDB Length = 181 Score = 53.1 bits (126), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 38/126 (30%), Positives = 57/126 (45%), Gaps = 3/126 (2%) Query: 33 WYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLALN 92 WY +L+KPSW PP T+ IW L+ + I++ GF L+ N Sbjct: 59 WYDALEKPSWTPPGMTIGMIWAVLFGLIALSVAIIYNN-YGFKPKTF--WFLFLLNYIFN 115 Query: 93 WAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLNY 152 A+ F + + A D LLV+ + +S +A LL PY W AFAT L++ Sbjct: 116 QAFSYFQFSQKNLFLATVDCLLVAITTLLLIMFSSNLSKVSAWLLIPYFLWSAFATYLSW 175 Query: 153 YVWRDN 158 ++ N Sbjct: 176 TIYSIN 181 >4XGV-A nrPDB Length = 340 Score = 28.1 bits (61), Expect = 2.2, Method: Compositional matrix adjust. Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 1/58 (1%) Query: 16 SLGGFMGAYFVRGEGLRWYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGG 73 S+GG + + + GEGL W ++QKP+ A + + GSY + EL G Sbjct: 191 SVGGALNSRGMNGEGLPWRVAIQKPT-DKENAVQAVVDINGHGISTSGSYRNYYELDG 247 >2A5Y-A nrPDB Length = 204 Score = 26.6 bits (57), Expect = 5.6, Method: Compositional matrix adjust. Identities = 13/58 (22%), Positives = 26/58 (44%), Gaps = 3/58 (5%) Query: 16 SLGGFMGAYF---VRGEGLRWYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKE 70 + GF+ YF +R G+ W+ + PS P + + GT++ ++ + E Sbjct: 32 DIEGFVVDYFTHRIRQNGMEWFGAPGLPSGVQPEHEMMRVMGTIFEKKHAENFETFSE 89 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2MGY-A nrPDB 222 5e-59 4RYM-A nrPDB 134 3e-32 4UC1-A nrPDB 130 3e-31 Sequences not found previously or not previously below threshold: 4XGV-A nrPDB 28 2.1 1MHS-A nrPDB 27 4.9 5HQA-A nrPDB 26 7.7 CONVERGED! >2MGY-A nrPDB Length = 169 Score = 222 bits (566), Expect = 5e-59, Method: Composition-based stats. Identities = 167/169 (98%), Positives = 167/169 (98%) Query: 1 MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYASLQKPSWHPPRWTLAPIWGTLYSAM 60 MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYA LQKPSWHPPRWTLAPIWGTLYSAM Sbjct: 1 MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYAGLQKPSWHPPRWTLAPIWGTLYSAM 60 Query: 61 GYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVAT 120 GYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVAT Sbjct: 61 GYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVAT 120 Query: 121 ATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRLPE 169 ATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRL E Sbjct: 121 ATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRLAE 169 >4RYM-A nrPDB Length = 181 Score = 134 bits (336), Expect = 3e-32, Method: Composition-based stats. Identities = 38/126 (30%), Positives = 57/126 (45%), Gaps = 3/126 (2%) Query: 33 WYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLALN 92 WY +L+KPSW PP T+ IW L+ + I++ GF L+ N Sbjct: 59 WYDALEKPSWTPPGMTIGMIWAVLFGLIALSVAIIYNN-YGFKPKTF--WFLFLLNYIFN 115 Query: 93 WAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLNY 152 A+ F + + A D LLV+ + +S +A LL PY W AFAT L++ Sbjct: 116 QAFSYFQFSQKNLFLATVDCLLVAITTLLLIMFSSNLSKVSAWLLIPYFLWSAFATYLSW 175 Query: 153 YVWRDN 158 ++ N Sbjct: 176 TIYSIN 181 >4UC1-A nrPDB Length = 157 Score = 130 bits (327), Expect = 3e-31, Method: Composition-based stats. Identities = 47/130 (36%), Positives = 64/130 (49%), Gaps = 5/130 (3%) Query: 32 RWYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLAL 91 WY +L KP W+PPRW W +LY M + V +L G + L Y QLA Sbjct: 29 EWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRV-AQLEGSGQ----ALAFYAAQLAF 83 Query: 92 NWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLN 151 N W P+FFG ++M ALA ++++ AT A+ ++ A L PYL W T LN Sbjct: 84 NTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATATTGLN 143 Query: 152 YYVWRDNSGR 161 + R N R Sbjct: 144 FEAMRLNWNR 153 >4XGV-A nrPDB Length = 340 Score = 28.1 bits (61), Expect = 2.1, Method: Composition-based stats. Identities = 11/26 (42%), Positives = 18/26 (69%) Query: 16 SLGGFMGAYFVRGEGLRWYASLQKPS 41 S+GG + + + GEGL W ++QKP+ Sbjct: 191 SVGGALNSRGMNGEGLPWRVAIQKPT 216 >1MHS-A nrPDB Length = 920 Score = 26.9 bits (58), Expect = 4.9, Method: Composition-based stats. Identities = 20/121 (16%), Positives = 34/121 (28%), Gaps = 17/121 (14%) Query: 41 SWHPPRWTLAPIWGT---LYSAMGYGSYIVWKELGGFTEDA--------MVPLGLYTGQL 89 S P +W L +WG L + G++I + E+ M + L Sbjct: 744 SQTPVKWNLPKLWGMSVLLGVVLAVGTWITVTTMYAQGENGGIVQNFGNMDEVLFLQISL 803 Query: 90 ALNWAW------PPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAW 143 NW P + + A L+ T W S + + + Sbjct: 804 TENWLIFITRANGPFWSSIPSWQLSGAIFLVDILATCFTIWGWFEHSDTSIVAVVRIWIF 863 Query: 144 L 144 Sbjct: 864 S 864 >5HQA-A nrPDB Length = 669 Score = 26.2 bits (56), Expect = 7.7, Method: Composition-based stats. Identities = 11/60 (18%), Positives = 24/60 (40%), Gaps = 3/60 (5%) Query: 58 SAMGYGSYIVWKELGGFTEDAMVPLGLYTG---QLALNWAWPPIFFGARQMGWALADLLL 114 + + Y + ++ + G + + P Q A N W I G + + +D++L Sbjct: 188 ALVDYAAMVLNQRRPGVFQADLTPWSSGVAVKKQGAFNTPWRTIQIGEKAVDLVNSDIIL 247 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.134 0.434 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,751,919 Number of Sequences: 36641 Number of extensions: 304385 Number of successful extensions: 600 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 585 Number of HSP's gapped (non-prelim): 25 length of query: 169 length of database: 10,336,785 effective HSP length: 90 effective length of query: 79 effective length of database: 7,039,095 effective search space: 556088505 effective search space used: 556088505 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)