BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P50135 (292 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2AOV-A nrPDB 608 e-175 4LFI-A nrPDB 29 2.1 4KRI-A nrPDB 28 3.3 1NKV-A nrPDB 28 3.5 3EY8-A nrPDB 28 5.5 1Y5E-A nrPDB 27 8.7 >2AOV-A nrPDB Length = 292 Score = 608 bits (1568), Expect = e-175, Method: Compositional matrix adjust. Identities = 292/292 (100%), Positives = 292/292 (100%) Query: 1 MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIG 60 MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIG Sbjct: 1 MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIG 60 Query: 61 GGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETS 120 GGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETS Sbjct: 61 GGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETS 120 Query: 121 SEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWD 180 SEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWD Sbjct: 121 SEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWD 180 Query: 181 KLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLL 240 KLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLL Sbjct: 181 KLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLL 240 Query: 241 WDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA 292 WDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA Sbjct: 241 WDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA 292 >4LFI-A nrPDB Length = 374 Score = 29.3 bits (64), Expect = 2.1, Method: Compositional matrix adjust. Identities = 27/109 (24%), Positives = 50/109 (45%), Gaps = 24/109 (22%) Query: 133 LQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQ 192 L W F M+ + + ++ FFH T+ + I+ V G+S ++K KY P+ Sbjct: 254 LDLWSFGTMLASMIFKRE-----PFFHGTSNTDQLVKIVKVLGTSDFEKYLLKYEITLPR 308 Query: 193 D--DLCQYIT----------------SDDLTQMLDNLGLKYECYDLLST 223 + D+ QYI +D++ ++DNL L+Y+ + L+ Sbjct: 309 EFYDMDQYIRKPWHRFINDGNKHLSGNDEIIDLIDNL-LRYDHQERLTA 356 >4KRI-A nrPDB Length = 433 Score = 28.5 bits (62), Expect = 3.3, Method: Compositional matrix adjust. Identities = 29/137 (21%), Positives = 53/137 (38%), Gaps = 16/137 (11%) Query: 42 GIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKEL 101 I+ R G K+ ++L IG G G Q S+ Q GV ++ ++ + E++ K K+ Sbjct: 212 AILKRFGPMKTGQRMLDIGVGIGGGARQAASEFGLQVHGVDLSTNMLAVALERVHKEKD- 270 Query: 102 VAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSL 161 V +A E+ E +D++ + ++KD + Sbjct: 271 -------ARVTYAVCDACEYEF--------EPNSFDYVFSRDCIQHIKDTDKLFSRIYRA 315 Query: 162 LGTNAKMLIIVVSGSSG 178 L K+LI + G Sbjct: 316 LKPGGKVLITMYGVGHG 332 >1NKV-A nrPDB Length = 256 Score = 28.5 bits (62), Expect = 3.5, Method: Compositional matrix adjust. Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Query: 20 RRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEI 66 R F +EH+ F ++K +GR+ K +IL +G G+GE+ Sbjct: 5 RIFTISESEHRIHNPFTEEKY-ATLGRVLRMKPGTRILDLGSGSGEM 50 >3EY8-A nrPDB Length = 133 Score = 27.7 bits (60), Expect = 5.5, Method: Compositional matrix adjust. Identities = 11/27 (40%), Positives = 16/27 (59%) Query: 140 HMIQMLYYVKDIPATLKFFHSLLGTNA 166 H+ ++ V DIP T KF+ +LG A Sbjct: 10 HLDHLVLTVADIPTTTKFYEKVLGMKA 36 >1Y5E-A nrPDB Length = 169 Score = 27.3 bits (59), Expect = 8.7, Method: Compositional matrix adjust. Identities = 16/66 (24%), Positives = 36/66 (54%), Gaps = 3/66 (4%) Query: 117 KETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGS 176 ++ + E S +L+K + F + +M+ Y++DI ++ ++ GT + ++ + GS Sbjct: 87 RDVTIEAVSALLDK---EIVGFGELFRMISYLEDIGSSAMLSRAIGGTIGRKVVFSMPGS 143 Query: 177 SGWDKL 182 SG +L Sbjct: 144 SGAVRL 149 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2AOV-A nrPDB 628 e-180 Sequences not found previously or not previously below threshold: 4LFI-A nrPDB 33 0.17 1NKV-A nrPDB 29 2.3 2LJK-A nrPDB 28 3.6 3EY8-A nrPDB 28 3.7 5TOO-A nrPDB 28 3.8 2PBF-A nrPDB 28 4.8 6O6Q-A nrPDB 27 7.6 2VCT-A nrPDB 27 7.6 3OFN-A nrPDB 27 7.6 5A0Z-A nrPDB 27 8.1 4KRI-A nrPDB 27 9.5 CONVERGED! >2AOV-A nrPDB Length = 292 Score = 628 bits (1619), Expect = e-180, Method: Composition-based stats. Identities = 292/292 (100%), Positives = 292/292 (100%) Query: 1 MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIG 60 MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIG Sbjct: 1 MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIG 60 Query: 61 GGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETS 120 GGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETS Sbjct: 61 GGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETS 120 Query: 121 SEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWD 180 SEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWD Sbjct: 121 SEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWD 180 Query: 181 KLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLL 240 KLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLL Sbjct: 181 KLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLL 240 Query: 241 WDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA 292 WDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA Sbjct: 241 WDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA 292 >4LFI-A nrPDB Length = 374 Score = 32.7 bits (73), Expect = 0.17, Method: Composition-based stats. Identities = 27/111 (24%), Positives = 51/111 (45%), Gaps = 24/111 (21%) Query: 133 LQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQ 192 L W F M+ + + ++ FFH T+ + I+ V G+S ++K KY P+ Sbjct: 254 LDLWSFGTMLASMIFKRE-----PFFHGTSNTDQLVKIVKVLGTSDFEKYLLKYEITLPR 308 Query: 193 D--DLCQYIT----------------SDDLTQMLDNLGLKYECYDLLSTMD 225 + D+ QYI +D++ ++DNL L+Y+ + L+ + Sbjct: 309 EFYDMDQYIRKPWHRFINDGNKHLSGNDEIIDLIDNL-LRYDHQERLTAKE 358 >1NKV-A nrPDB Length = 256 Score = 29.2 bits (64), Expect = 2.3, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Query: 20 RRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEI 66 R F +EH+ F ++K +GR+ K +IL +G G+GE+ Sbjct: 5 RIFTISESEHRIHNPFTEEKY-ATLGRVLRMKPGTRILDLGSGSGEM 50 >2LJK-A nrPDB Length = 117 Score = 28.5 bits (62), Expect = 3.6, Method: Composition-based stats. Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 2/51 (3%) Query: 68 LQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKE 118 L++ S V+ QYPG+ I + + A +I +LV S LEN F + K+ Sbjct: 42 LELASAVKEQYPGIEIESRLGGTGAFEIEINGQLV--FSKLENGGFPYEKD 90 >3EY8-A nrPDB Length = 133 Score = 28.5 bits (62), Expect = 3.7, Method: Composition-based stats. Identities = 11/27 (40%), Positives = 16/27 (59%) Query: 140 HMIQMLYYVKDIPATLKFFHSLLGTNA 166 H+ ++ V DIP T KF+ +LG A Sbjct: 10 HLDHLVLTVADIPTTTKFYEKVLGMKA 36 >5TOO-A nrPDB Length = 549 Score = 28.5 bits (62), Expect = 3.8, Method: Composition-based stats. Identities = 38/140 (27%), Positives = 59/140 (42%), Gaps = 29/140 (20%) Query: 115 WHKETSSEYQSRMLEKKELQKW--DFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIV 172 W +T+ ++ + KEL KW DF + K++PA L G N + I Sbjct: 168 WFDDTTGKFITSTYYTKELPKWVNDFNN--------KNVPAQL----VANGWNTLLPINQ 215 Query: 173 VSGSSGWDKLWK-----KYGSRFPQDDLCQ-YITSDDLTQMLDNLGLKYECYDLLSTMDI 226 + SS + W+ K FP DL + Y L ++ + T+ + Sbjct: 216 YTESSEDNVEWEGLLGSKKTPTFPYTDLAKDYEAKKGL--------IRTTPFGNTLTLQM 267 Query: 227 SDCFIDGNENG-DLLWDFLT 245 +D IDGN+ G D + DFLT Sbjct: 268 ADAAIDGNQMGVDDITDFLT 287 >2PBF-A nrPDB Length = 227 Score = 28.1 bits (61), Expect = 4.8, Method: Composition-based stats. Identities = 32/122 (26%), Positives = 52/122 (42%), Gaps = 19/122 (15%) Query: 55 KILSIGGGAG------EIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNL 108 + + +G G+G I + +L + G+ ++V S E I + K + K N Sbjct: 83 RAIDVGSGSGYLTVCMAIKMNVLENKNSYVIGLERVKDLVNFSLENIKRDKPELLKIDNF 142 Query: 109 ENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKM 168 + + HK YQ EKKEL +D IH+ ++P L LL N K+ Sbjct: 143 KII----HKNI---YQVNEEEKKELGLFDAIHVGAS---ASELPEILV---DLLAENGKL 189 Query: 169 LI 170 +I Sbjct: 190 II 191 >6O6Q-A nrPDB Length = 335 Score = 27.3 bits (59), Expect = 7.6, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 5/54 (9%) Query: 133 LQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKY 186 L W F M+ + ++K+ FFH T+ + I+ V GS + K +KY Sbjct: 217 LDMWSFGCMLASMVFMKE-----PFFHGKSNTDQLVQIVRVLGSKNFKKYLEKY 265 >2VCT-A nrPDB Length = 222 Score = 27.3 bits (59), Expect = 7.6, Method: Composition-based stats. Identities = 21/63 (33%), Positives = 35/63 (55%), Gaps = 4/63 (6%) Query: 93 EQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIP 152 EQ AK + KT N A+ K S Q ++ K L + D IH++++LYYV+++ Sbjct: 116 EQDAKLALIQEKTKN--RYFPAFEKVLKSHGQDYLVGNK-LSRAD-IHLVELLYYVEELD 171 Query: 153 ATL 155 ++L Sbjct: 172 SSL 174 >3OFN-A nrPDB Length = 510 Score = 27.3 bits (59), Expect = 7.6, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 25/53 (47%), Gaps = 11/53 (20%) Query: 41 PGIIGRIGDTKSEIKILSIGG---GAGEIDLQILSKVQAQYPGVCINNEVVEP 90 PG++GR+ D ++G G G ID S+ Q + PG+ V EP Sbjct: 103 PGLLGRVVD--------ALGNPIDGKGPIDAAGRSRAQVKAPGILPRRSVHEP 147 >5A0Z-A nrPDB Length = 792 Score = 27.3 bits (59), Expect = 8.1, Method: Composition-based stats. Identities = 16/67 (23%), Positives = 35/67 (52%), Gaps = 6/67 (8%) Query: 70 ILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLE 129 IL Q Q+ V +NEV++ + ++ KY++L+ + + A+ E E Q ++ Sbjct: 732 ILGNGQMQFSYV--DNEVLKKAQQEPEKYRDLIVRVAGYS----AYFVELCKEVQDEIIS 785 Query: 130 KKELQKW 136 + ++K+ Sbjct: 786 RTVIEKF 792 >4KRI-A nrPDB Length = 433 Score = 26.9 bits (58), Expect = 9.5, Method: Composition-based stats. Identities = 32/149 (21%), Positives = 60/149 (40%), Gaps = 17/149 (11%) Query: 42 GIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKEL 101 I+ R G K+ ++L IG G G Q S+ Q GV ++ ++ + E++ K K+ Sbjct: 212 AILKRFGPMKTGQRMLDIGVGIGGGARQAASEFGLQVHGVDLSTNMLAVALERVHKEKD- 270 Query: 102 VAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSL 161 V +A E+ E +D++ + ++KD + Sbjct: 271 -------ARVTYAVCDACEYEF--------EPNSFDYVFSRDCIQHIKDTDKLFSRIYRA 315 Query: 162 LGTNAKMLIIVVS-GSSGWDKLWKKYGSR 189 L K+LI + G + +K+Y S+ Sbjct: 316 LKPGGKVLITMYGVGHGTLSESFKEYVSQ 344 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.135 0.401 Lambda K H 0.267 0.0437 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,202,008 Number of Sequences: 36641 Number of extensions: 589270 Number of successful extensions: 1453 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 1445 Number of HSP's gapped (non-prelim): 29 length of query: 292 length of database: 10,336,785 effective HSP length: 96 effective length of query: 196 effective length of database: 6,819,249 effective search space: 1336572804 effective search space used: 1336572804 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.2 bits)