BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P50106 (137 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5W5Y-D nrPDB 276 1e-75 4D2I-A nrPDB 30 0.40 4V6W-AS nrPDB 29 0.56 2EP5-A nrPDB 29 0.70 3FIP-A nrPDB 28 0.89 6OD8-A nrPDB 28 1.5 6CD2-C nrPDB 28 1.5 5NW4-V nrPDB 27 2.1 6GIO-D nrPDB 27 2.8 3L46-A nrPDB 27 3.7 1BF6-A nrPDB 26 4.2 4ZKU-A nrPDB 26 5.2 6MO6-A nrPDB 25 8.7 4B93-A nrPDB 25 9.4 >5W5Y-D nrPDB Length = 137 Score = 276 bits (707), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 137/137 (100%), Positives = 137/137 (100%) Query: 1 MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS 60 MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS Sbjct: 1 MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS 60 Query: 61 GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET 120 GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET Sbjct: 61 GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET 120 Query: 121 SIGVSATGGKKTTFADE 137 SIGVSATGGKKTTFADE Sbjct: 121 SIGVSATGGKKTTFADE 137 >4D2I-A nrPDB Length = 500 Score = 29.6 bits (65), Expect = 0.40, Method: Compositional matrix adjust. Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Query: 32 STDEVLQFLESFIDEKENIIDSTTMNTISGNAADADAAAVANTSLNIDTNLSSSISQLKR 91 + DEVL E F+D N+ID T+ + I V+ T L+ D+ + L+R Sbjct: 272 AKDEVLNKFEEFMDRYSNVIDLTSSDIIE-KVKRGKVNVVSLTQLDEDSMDAVVSHYLRR 330 Query: 92 I---QRDFK 97 I ++DFK Sbjct: 331 ILDSRKDFK 339 >4V6W-AS nrPDB Length = 152 Score = 29.3 bits (64), Expect = 0.56, Method: Compositional matrix adjust. Identities = 17/59 (28%), Positives = 27/59 (45%), Gaps = 1/59 (1%) Query: 67 DAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETSIGVS 125 D TS N+D+ L + +LK+I R +GL TT ++ ++GVS Sbjct: 92 DGKYWQLTSSNLDSKLRDDLERLKKI-RSHRGLRHYWGLRVRGQHTKTTGRRGRTVGVS 149 >2EP5-A nrPDB Length = 350 Score = 28.9 bits (63), Expect = 0.70, Method: Compositional matrix adjust. Identities = 13/36 (36%), Positives = 22/36 (61%) Query: 76 LNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQ 111 +NI TN +I ++K+ ++FK LP ++ AP Q Sbjct: 248 INIVTNERINIEEIKKTLKNFKSLPQQKNLPTAPKQ 283 >3FIP-A nrPDB Length = 493 Score = 28.5 bits (62), Expect = 0.89, Method: Composition-based stats. Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 3/59 (5%) Query: 75 SLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETSIGVSATGGKKTT 133 SLN N ++ ++I + P + SA +++ +K TS GVSA+GG T Sbjct: 438 SLNAGLNSGGGLTSQRQINAYYSHRSPLANLSA---NIASLQKGYTSFGVSASGGATIT 493 >6OD8-A nrPDB Length = 558 Score = 27.7 bits (60), Expect = 1.5, Method: Composition-based stats. Identities = 23/69 (33%), Positives = 33/69 (47%), Gaps = 2/69 (2%) Query: 59 ISGNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQD--FSAAPIQVSTTE 116 +S N ADA A AVA + + ++ +++ K + L F AAP+ STT Sbjct: 9 MSANHADAGAPAVAKKMSDKEARKAARLAEEKARADEKAALVEKYKAVFGAAPMVQSTTY 68 Query: 117 KKETSIGVS 125 K T I VS Sbjct: 69 KSRTHIPVS 77 >6CD2-C nrPDB Length = 757 Score = 27.7 bits (60), Expect = 1.5, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 3/55 (5%) Query: 75 SLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETSIGVSATGG 129 SLN N ++ ++I + P + SA +++ +K TS GVSA+GG Sbjct: 569 SLNAGLNSGGGLTSQRQINAYYSHRSPLANLSA---NIASLQKGYTSFGVSASGG 620 >5NW4-V nrPDB Length = 396 Score = 27.3 bits (59), Expect = 2.1, Method: Compositional matrix adjust. Identities = 22/89 (24%), Positives = 39/89 (43%), Gaps = 6/89 (6%) Query: 39 FLESFIDEKENIIDSTTMNTISGNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKG 98 F SF+ + I TT N+I D ANT L+ T + I+ R+Q++ Sbjct: 283 FQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIAD--RMQKEITA 340 Query: 99 LPPAQDFSAAPIQVSTTEKKETSIGVSAT 127 L P S I++ +++ S+ + + Sbjct: 341 LAP----STMKIKIIAPPERKYSVWIGGS 365 >6GIO-D nrPDB Length = 439 Score = 26.9 bits (58), Expect = 2.8, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 17/33 (51%) Query: 37 LQFLESFIDEKENIIDSTTMNTISGNAADADAA 69 L E IDE +I+D + + G AD+D A Sbjct: 402 LTLTEPEIDEAADIVDQAIGDVLDGKVADSDVA 434 >3L46-A nrPDB Length = 112 Score = 26.6 bits (57), Expect = 3.7, Method: Compositional matrix adjust. Identities = 14/42 (33%), Positives = 18/42 (42%) Query: 96 FKGLPPAQDFSAAPIQVSTTEKKETSIGVSATGGKKTTFADE 137 F+G+PP QD + + S EK GGK DE Sbjct: 16 FQGVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDE 57 >1BF6-A nrPDB Length = 291 Score = 26.2 bits (56), Expect = 4.2, Method: Compositional matrix adjust. Identities = 11/30 (36%), Positives = 17/30 (56%) Query: 27 LPQHVSTDEVLQFLESFIDEKENIIDSTTM 56 P+HV+T V + + +DE E ID T + Sbjct: 88 FPEHVATRSVQELAQEMVDEIEQGIDGTEL 117 >4ZKU-A nrPDB Length = 237 Score = 26.2 bits (56), Expect = 5.2, Method: Compositional matrix adjust. Identities = 35/133 (26%), Positives = 61/133 (45%), Gaps = 23/133 (17%) Query: 15 TLNTPVVIHATQ-----LPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISGNAADADAA 69 +LN PVVI AT+ LP++V + L ++ I T + I+G A +A Sbjct: 9 SLNNPVVIQATRLDASILPRNVFSKSYLLYV---------IAQGTDVGAIAGKANEAGQG 59 Query: 70 AVANTSLN--IDTNLSSSISQLKRIQRDF-----KGLPPAQDFSAAPIQVSTTEKKETSI 122 A N D L+ +++K+++ D + + +A ++V+T E + S+ Sbjct: 60 AYDAQVKNDEQDVELADHEARIKQLRIDVDDHESRITANTKAITALNVRVTTAEGEIASL 119 Query: 123 --GVSATGGKKTT 133 VSA G+ TT Sbjct: 120 QTNVSALDGRVTT 132 >6MO6-A nrPDB Length = 414 Score = 25.4 bits (54), Expect = 8.7, Method: Composition-based stats. Identities = 22/84 (26%), Positives = 35/84 (41%), Gaps = 2/84 (2%) Query: 7 RTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISGNAADA 66 + GN T NTPV PQ + F ++ K NII +T++ I G A Sbjct: 146 KEGNAIFTFPNTPV--KCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYA 203 Query: 67 DAAAVANTSLNIDTNLSSSISQLK 90 DA N+ N ++ +++ Sbjct: 204 DALQEIIQERNLTVNYKKNLIEVR 227 >4B93-A nrPDB Length = 189 Score = 25.0 bits (53), Expect = 9.4, Method: Compositional matrix adjust. Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 1/28 (3%) Query: 40 LESFIDEKENIIDST-TMNTISGNAADA 66 LE ID+ EN++DS+ T T S N A A Sbjct: 155 LELLIDKTENLVDSSVTFKTTSRNLARA 182 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5W5Y-D nrPDB 243 2e-65 Sequences not found previously or not previously below threshold: 4D2I-A nrPDB 33 0.041 2EP5-A nrPDB 33 0.047 3FIP-A nrPDB 32 0.10 6CD2-C nrPDB 31 0.21 6OD8-A nrPDB 31 0.23 6GAW-Ah nrPDB 29 0.64 1BF6-A nrPDB 29 0.66 4ZKU-A nrPDB 29 0.73 5UDD-A nrPDB 29 0.91 6GIO-D nrPDB 28 1.0 6MO6-A nrPDB 27 2.3 4R9I-A nrPDB 27 2.6 4WE2-A nrPDB 26 7.4 CONVERGED! >5W5Y-D nrPDB Length = 137 Score = 243 bits (619), Expect = 2e-65, Method: Composition-based stats. Identities = 137/137 (100%), Positives = 137/137 (100%) Query: 1 MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS 60 MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS Sbjct: 1 MMKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTIS 60 Query: 61 GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET 120 GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET Sbjct: 61 GNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKET 120 Query: 121 SIGVSATGGKKTTFADE 137 SIGVSATGGKKTTFADE Sbjct: 121 SIGVSATGGKKTTFADE 137 >4D2I-A nrPDB Length = 500 Score = 32.9 bits (73), Expect = 0.041, Method: Composition-based stats. Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Query: 32 STDEVLQFLESFIDEKENIIDSTTMNTISGNAADADAAAVANTSLNIDTNLSSSISQLKR 91 + DEVL E F+D N+ID T+ + I V+ T L+ D+ + L+R Sbjct: 272 AKDEVLNKFEEFMDRYSNVIDLTSSDIIE-KVKRGKVNVVSLTQLDEDSMDAVVSHYLRR 330 Query: 92 I---QRDFK 97 I ++DFK Sbjct: 331 ILDSRKDFK 339 >2EP5-A nrPDB Length = 350 Score = 32.9 bits (73), Expect = 0.047, Method: Composition-based stats. Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 4/62 (6%) Query: 76 LNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETS----IGVSATGGKK 131 +NI TN +I ++K+ ++FK LP ++ AP Q +E I V+A G Sbjct: 248 INIVTNERINIEEIKKTLKNFKSLPQQKNLPTAPKQPIIVRDEEDRPQPIIDVNAESGMA 307 Query: 132 TT 133 T Sbjct: 308 VT 309 >3FIP-A nrPDB Length = 493 Score = 31.7 bits (70), Expect = 0.10, Method: Composition-based stats. Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 3/59 (5%) Query: 75 SLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETSIGVSATGGKKTT 133 SLN N ++ ++I + P + SA +++ +K TS GVSA+GG T Sbjct: 438 SLNAGLNSGGGLTSQRQINAYYSHRSPLANLSA---NIASLQKGYTSFGVSASGGATIT 493 >6CD2-C nrPDB Length = 757 Score = 30.6 bits (67), Expect = 0.21, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 3/55 (5%) Query: 75 SLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETSIGVSATGG 129 SLN N ++ ++I + P + SA +++ +K TS GVSA+GG Sbjct: 569 SLNAGLNSGGGLTSQRQINAYYSHRSPLANLSA---NIASLQKGYTSFGVSASGG 620 >6OD8-A nrPDB Length = 558 Score = 30.6 bits (67), Expect = 0.23, Method: Composition-based stats. Identities = 23/69 (33%), Positives = 33/69 (47%), Gaps = 2/69 (2%) Query: 59 ISGNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQD--FSAAPIQVSTTE 116 +S N ADA A AVA + + ++ +++ K + L F AAP+ STT Sbjct: 9 MSANHADAGAPAVAKKMSDKEARKAARLAEEKARADEKAALVEKYKAVFGAAPMVQSTTY 68 Query: 117 KKETSIGVS 125 K T I VS Sbjct: 69 KSRTHIPVS 77 >6GAW-Ah nrPDB Length = 387 Score = 29.0 bits (63), Expect = 0.64, Method: Composition-based stats. Identities = 31/127 (24%), Positives = 53/127 (41%), Gaps = 10/127 (7%) Query: 2 MKGSRRTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISG 61 ++G+ RT NN T ++++ + Q T E+ + ES KEN +N I G Sbjct: 37 LRGTVRTENNIQRYFGTNNLVYSKKDEQSGPTCEISKETESKGSVKEN-SKKDLLNIIKG 95 Query: 62 NAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFKGLPPAQDFSAAPIQVSTTEKKETS 121 V +++N+ T S+ QLK ++ + A + P + S + E Sbjct: 96 -------MKVELSTVNVQTTKPSNKRQLKNLETAIGRIKKAPE--DTPQKRSESLSSELV 146 Query: 122 IGVSATG 128 VSA Sbjct: 147 AAVSAVA 153 >1BF6-A nrPDB Length = 291 Score = 29.0 bits (63), Expect = 0.66, Method: Composition-based stats. Identities = 11/30 (36%), Positives = 17/30 (56%) Query: 27 LPQHVSTDEVLQFLESFIDEKENIIDSTTM 56 P+HV+T V + + +DE E ID T + Sbjct: 88 FPEHVATRSVQELAQEMVDEIEQGIDGTEL 117 >4ZKU-A nrPDB Length = 237 Score = 29.0 bits (63), Expect = 0.73, Method: Composition-based stats. Identities = 36/139 (25%), Positives = 63/139 (45%), Gaps = 23/139 (16%) Query: 9 GNNTATTLNTPVVIHATQ-----LPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISGNA 63 G+ +LN PVVI AT+ LP++V + L ++ I T + I+G A Sbjct: 3 GSMADPSLNNPVVIQATRLDASILPRNVFSKSYLLYV---------IAQGTDVGAIAGKA 53 Query: 64 ADADAAAVANTSLN--IDTNLSSSISQLKRIQRDF-----KGLPPAQDFSAAPIQVSTTE 116 +A A N D L+ +++K+++ D + + +A ++V+T E Sbjct: 54 NEAGQGAYDAQVKNDEQDVELADHEARIKQLRIDVDDHESRITANTKAITALNVRVTTAE 113 Query: 117 KKETSI--GVSATGGKKTT 133 + S+ VSA G+ TT Sbjct: 114 GEIASLQTNVSALDGRVTT 132 >5UDD-A nrPDB Length = 568 Score = 28.6 bits (62), Expect = 0.91, Method: Composition-based stats. Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 6/71 (8%) Query: 33 TDEVLQFLESFIDEKENIIDST---TMNT-ISGNAADADAAAVANTSLNIDTNLSSSISQ 88 TD ++ ++ +D+ +N + T NT + N A +A N ++N NL+ SIS Sbjct: 72 TDTKVKLIKQELDKYKNAVTELQLLTQNTPAANNRARREAPQYMNYTINTTKNLNVSIS- 130 Query: 89 LKRIQRDFKGL 99 K+ +R F G Sbjct: 131 -KKRKRRFLGF 140 >6GIO-D nrPDB Length = 439 Score = 28.2 bits (61), Expect = 1.0, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 21/40 (52%), Gaps = 6/40 (15%) Query: 36 VLQFL------ESFIDEKENIIDSTTMNTISGNAADADAA 69 VL+F+ E IDE +I+D + + G AD+D A Sbjct: 395 VLEFMPPLTLTEPEIDEAADIVDQAIGDVLDGKVADSDVA 434 >6MO6-A nrPDB Length = 414 Score = 27.1 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 22/84 (26%), Positives = 35/84 (41%), Gaps = 2/84 (2%) Query: 7 RTGNNTATTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISGNAADA 66 + GN T NTPV PQ + F ++ K NII +T++ I G A Sbjct: 146 KEGNAIFTFPNTPV--KCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYA 203 Query: 67 DAAAVANTSLNIDTNLSSSISQLK 90 DA N+ N ++ +++ Sbjct: 204 DALQEIIQERNLTVNYKKNLIEVR 227 >4R9I-A nrPDB Length = 378 Score = 27.1 bits (58), Expect = 2.6, Method: Composition-based stats. Identities = 20/67 (29%), Positives = 28/67 (41%), Gaps = 4/67 (5%) Query: 14 TTLNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENIIDSTTMNTISGNAADADAAAVAN 73 T LNT ++ + L + TD F E+FI + + T N N DA A Sbjct: 88 TNLNTKNEVYQSTLILNAYTDIDSPFSETFIQNFAKVFNGTVKNIDYSN----DAVATIR 143 Query: 74 TSLNIDT 80 SL D+ Sbjct: 144 DSLQSDS 150 >4WE2-A nrPDB Length = 277 Score = 25.5 bits (54), Expect = 7.4, Method: Composition-based stats. Identities = 15/62 (24%), Positives = 26/62 (41%) Query: 38 QFLESFIDEKENIIDSTTMNTISGNAADADAAAVANTSLNIDTNLSSSISQLKRIQRDFK 97 + F D I T+SG A + +AA A T L + + + Q++R+ + Sbjct: 137 ELFSLFADGSRAIFYGGLTTTVSGAALTSGSAAAARTELFGSLSRNDILGQIQRVNANIT 196 Query: 98 GL 99 L Sbjct: 197 SL 198 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.122 0.319 Lambda K H 0.267 0.0389 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,229,706 Number of Sequences: 36641 Number of extensions: 130110 Number of successful extensions: 368 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 352 Number of HSP's gapped (non-prelim): 37 length of query: 137 length of database: 10,336,785 effective HSP length: 87 effective length of query: 50 effective length of database: 7,149,018 effective search space: 357450900 effective search space used: 357450900 T: 11 A: 40 X1: 15 ( 6.7 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 53 (25.1 bits)