BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P49768 (467 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5FN4-B nrPDB 966 0.0 2KR6-A nrPDB 367 e-102 4UIS-B nrPDB 78 9e-15 4BTS-AY nrPDB 29 3.7 >5FN4-B nrPDB Length = 467 Score = 966 bits (2498), Expect = 0.0, Method: Compositional matrix adjust. Identities = 467/467 (100%), Positives = 467/467 (100%) Query: 1 MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSR 60 MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSR Sbjct: 1 MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSR 60 Query: 61 QVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTE 120 QVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTE Sbjct: 61 QVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTE 120 Query: 121 DTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFI 180 DTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFI Sbjct: 121 DTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFI 180 Query: 181 YLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKY 240 YLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKY Sbjct: 181 YLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKY 240 Query: 241 LPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAE 300 LPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAE Sbjct: 241 LPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAE 300 Query: 301 GDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAA 360 GDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAA Sbjct: 301 GDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAA 360 Query: 361 VQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL 420 VQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL Sbjct: 361 VQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL 420 Query: 421 TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 467 TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI Sbjct: 421 TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 467 >2KR6-A nrPDB Length = 176 Score = 367 bits (943), Expect = e-102, Method: Compositional matrix adjust. Identities = 176/176 (100%), Positives = 176/176 (100%) Query: 292 MVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPH 351 MVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPH Sbjct: 1 MVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPH 60 Query: 352 RSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACF 411 RSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACF Sbjct: 61 RSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACF 120 Query: 412 VAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 467 VAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI Sbjct: 121 VAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 176 >4UIS-B nrPDB Length = 241 Score = 77.8 bits (190), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 41/60 (68%), Positives = 41/60 (68%), Gaps = 1/60 (1%) Query: 404 WNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFH 463 WNTTIACFVAILIGLCLTLL GLVFYFATDYLVQPF DQLAFH Sbjct: 181 WNTTIACFVAILIGLCLTLLXXXXXXXXXXXXXXXXX-GLVFYFATDYLVQPFADQLAFH 239 Score = 54.7 bits (130), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 32/56 (57%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Query: 178 SFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALM 233 SFIYLGEVFKTY LIWNFG RLQQAYLIMISALM Sbjct: 66 SFIYLGEVFKTYXXXXX-XXXXXLIWNFGXXXXXXXXXXXXXRLQQAYLIMISALM 120 Score = 41.6 bits (96), Expect = 7e-04, Method: Compositional matrix adjust. Identities = 20/20 (100%), Positives = 20/20 (100%) Query: 81 HVIMLFVPVTLCMVVVVATI 100 HVIMLFVPVTLCMVVVVATI Sbjct: 13 HVIMLFVPVTLCMVVVVATI 32 >4BTS-AY nrPDB Length = 293 Score = 29.3 bits (64), Expect = 3.7, Method: Compositional matrix adjust. Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 10/71 (14%) Query: 284 PALIYSSTMVWLVNMAEGD-----PEAQR-----RVSKNSKYNAESTERESQDTVAENDD 333 P LI S + A+G P+ QR R+ + Y AE T+R + A+ + Sbjct: 153 PILIKKSVIRRTFKTAKGKDRTKCPKIQRLITPERILRKKVYKAEKTQRYVKTNAAKEEY 212 Query: 334 GGFSEEWEAQR 344 F EW+ QR Sbjct: 213 EKFLSEWKKQR 223 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5FN4-B nrPDB 919 0.0 2KR6-A nrPDB 348 3e-96 4UIS-B nrPDB 82 4e-16 Sequences not found previously or not previously below threshold: 4BTS-AY nrPDB 30 2.6 5JPN-B nrPDB 28 8.7 4XAM-C nrPDB 28 8.7 CONVERGED! >5FN4-B nrPDB Length = 467 Score = 919 bits (2375), Expect = 0.0, Method: Composition-based stats. Identities = 467/467 (100%), Positives = 467/467 (100%) Query: 1 MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSR 60 MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSR Sbjct: 1 MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSR 60 Query: 61 QVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTE 120 QVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTE Sbjct: 61 QVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTE 120 Query: 121 DTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFI 180 DTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFI Sbjct: 121 DTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFI 180 Query: 181 YLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKY 240 YLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKY Sbjct: 181 YLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKY 240 Query: 241 LPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAE 300 LPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAE Sbjct: 241 LPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAE 300 Query: 301 GDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAA 360 GDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAA Sbjct: 301 GDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAA 360 Query: 361 VQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL 420 VQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL Sbjct: 361 VQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL 420 Query: 421 TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 467 TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI Sbjct: 421 TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 467 >2KR6-A nrPDB Length = 176 Score = 348 bits (894), Expect = 3e-96, Method: Composition-based stats. Identities = 176/176 (100%), Positives = 176/176 (100%) Query: 292 MVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPH 351 MVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPH Sbjct: 1 MVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPH 60 Query: 352 RSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACF 411 RSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACF Sbjct: 61 RSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACF 120 Query: 412 VAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 467 VAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI Sbjct: 121 VAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI 176 >4UIS-B nrPDB Length = 241 Score = 82.4 bits (202), Expect = 4e-16, Method: Composition-based stats. Identities = 41/60 (68%), Positives = 41/60 (68%), Gaps = 1/60 (1%) Query: 404 WNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFH 463 WNTTIACFVAILIGLCLTLL GLVFYFATDYLVQPF DQLAFH Sbjct: 181 WNTTIACFVAILIGLCLTLLXXXXXXXXXXXX-XXXXXGLVFYFATDYLVQPFADQLAFH 239 Score = 58.5 bits (140), Expect = 5e-09, Method: Composition-based stats. Identities = 32/56 (57%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Query: 178 SFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALM 233 SFIYLGEVFKTY LIWNFG RLQQAYLIMISALM Sbjct: 66 SFIYLGEVFKTYXXXX-XXXXXXLIWNFGXXXXXXXXXXXXXRLQQAYLIMISALM 120 Score = 42.7 bits (99), Expect = 4e-04, Method: Composition-based stats. Identities = 20/20 (100%), Positives = 20/20 (100%) Query: 81 HVIMLFVPVTLCMVVVVATI 100 HVIMLFVPVTLCMVVVVATI Sbjct: 13 HVIMLFVPVTLCMVVVVATI 32 >4BTS-AY nrPDB Length = 293 Score = 30.0 bits (66), Expect = 2.6, Method: Composition-based stats. Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 10/71 (14%) Query: 284 PALIYSSTMVWLVNMAEGD-----PEAQR-----RVSKNSKYNAESTERESQDTVAENDD 333 P LI S + A+G P+ QR R+ + Y AE T+R + A+ + Sbjct: 153 PILIKKSVIRRTFKTAKGKDRTKCPKIQRLITPERILRKKVYKAEKTQRYVKTNAAKEEY 212 Query: 334 GGFSEEWEAQR 344 F EW+ QR Sbjct: 213 EKFLSEWKKQR 223 >5JPN-B nrPDB Length = 767 Score = 28.1 bits (61), Expect = 8.7, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 25/47 (53%) Query: 337 SEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKLGL 383 + W ++ S G RST ++ A+ LS+ +A EERG+ + L Sbjct: 606 ASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTL 652 >4XAM-C nrPDB Length = 690 Score = 28.1 bits (61), Expect = 8.7, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 25/47 (53%) Query: 337 SEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKLGL 383 + W ++ S G RST ++ A+ LS+ +A EERG+ + L Sbjct: 529 ASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTL 575 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.331 0.138 0.422 Lambda K H 0.267 0.0434 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,604,239 Number of Sequences: 36641 Number of extensions: 892370 Number of successful extensions: 2024 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 25 Number of HSP's that attempted gapping in prelim test: 1994 Number of HSP's gapped (non-prelim): 46 length of query: 467 length of database: 10,336,785 effective HSP length: 101 effective length of query: 366 effective length of database: 6,636,044 effective search space: 2428792104 effective search space used: 2428792104 T: 11 A: 40 X1: 16 ( 7.6 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.4 bits) S2: 61 (28.0 bits)