BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P47142 (202 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1XB4-A nrPDB 416 e-117 3CUQ-C nrPDB 92 2e-19 3HTU-A nrPDB 54 6e-08 2ZME-C nrPDB 46 1e-05 6Q8Y-z nrPDB 36 0.012 6GYP-E nrPDB 28 2.2 4WWA-A nrPDB 27 4.8 >1XB4-A nrPDB Length = 202 Score = 416 bits (1069), Expect = e-117, Method: Compositional matrix adjust. Identities = 202/202 (100%), Positives = 202/202 (100%) Query: 1 MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDS 60 MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDS Sbjct: 1 MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDS 60 Query: 61 GSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTR 120 GSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTR Sbjct: 61 GSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTR 120 Query: 121 YFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPL 180 YFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPL Sbjct: 121 YFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPL 180 Query: 181 CDRNRATMLKDENDKVIAIKVV 202 CDRNRATMLKDENDKVIAIKVV Sbjct: 181 CDRNRATMLKDENDKVIAIKVV 202 >3CUQ-C nrPDB Length = 176 Score = 91.7 bits (226), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 56/185 (30%), Positives = 91/185 (49%), Gaps = 27/185 (14%) Query: 5 PPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDSGSTD 64 P Y FPP +T QPN TR++Q++ W ++ +C+ K M TV+ E Sbjct: 8 PWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSM----TVMEAQE------- 56 Query: 65 NDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTRYFIL 124 LFNN +QR + I + ++ K+G +D+S + + I+ Sbjct: 57 --------SPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKS--------KSSFLIM 100 Query: 125 WKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPLCDRN 184 W+ + W LI QW SG+ N V TLYEL+ G++T + EFH + E+ L L+ L + Sbjct: 101 WRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEH 160 Query: 185 RATML 189 +A ++ Sbjct: 161 KAEII 165 >3HTU-A nrPDB Length = 79 Score = 53.5 bits (127), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 24/61 (39%), Positives = 37/61 (60%) Query: 129 DSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPLCDRNRATM 188 + W LI QW SG+ N V TLYEL+ G++T + EFH + E+ L L+ L ++A + Sbjct: 8 EEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEI 67 Query: 189 L 189 + Sbjct: 68 I 68 >2ZME-C nrPDB Length = 102 Score = 46.2 bits (108), Expect = 1e-05, Method: Compositional matrix adjust. Identities = 31/122 (25%), Positives = 53/122 (43%), Gaps = 27/122 (22%) Query: 5 PPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDSGSTD 64 P Y FPP +T QPN TR++Q++ W ++ +C+ K TV+ E Sbjct: 8 PWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHK----QSSMTVMEAQE------- 56 Query: 65 NDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTRYFIL 124 LFNN +QR + I + ++ K+G +D+S + + I+ Sbjct: 57 --------SPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSK--------SSFLIM 100 Query: 125 WK 126 W+ Sbjct: 101 WR 102 >6Q8Y-z nrPDB Length = 916 Score = 35.8 bits (81), Expect = 0.012, Method: Compositional matrix adjust. Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 4/63 (6%) Query: 26 QISTWIDIISQYCKTKKIWYMSVDGTV----INDNELDSGSTDNDDSKKISKNLFNNEDI 81 +I T+ID + Q K KKI+YM++DG +N T D K + K + N ++I Sbjct: 61 KICTYIDHLFQTIKPKKIFYMAIDGVAPRAKMNQQRARRFRTAMDAEKALKKAIENGDEI 120 Query: 82 QRS 84 + Sbjct: 121 PKG 123 >6GYP-E nrPDB Length = 956 Score = 28.5 bits (62), Expect = 2.2, Method: Compositional matrix adjust. Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Query: 105 PIDQSGRRSSNTTTTRYFILWKSLDSWASLILQWFEDSGK-LNQVI 149 P + + + + T + F+L KS+DS +IL+WF + K NQ + Sbjct: 826 PSPEQDQEAKSYTDEQEFMLDKSIDSIEGIILEWFTPNAKYANQCV 871 >4WWA-A nrPDB Length = 261 Score = 27.3 bits (59), Expect = 4.8, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Query: 85 VSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTRYFILWKSLDSWASLILQW 138 ++Q FID++ S +T + PI Q TTTT + L ++ DS I+++ Sbjct: 1 MTQEFIDKVSSYLTPDVDIAPISQGAEAIVFTTTTHPY-LPRAKDSHQKYIIKY 53 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1XB4-A nrPDB 335 9e-93 3CUQ-C nrPDB 256 4e-69 2ZME-C nrPDB 142 9e-35 3HTU-A nrPDB 124 2e-29 Sequences not found previously or not previously below threshold: 5XOM-A nrPDB 33 0.088 1SZ2-A nrPDB 30 0.91 2C4M-A nrPDB 30 0.92 1GWF-A nrPDB 29 1.4 CONVERGED! >1XB4-A nrPDB Length = 202 Score = 335 bits (858), Expect = 9e-93, Method: Composition-based stats. Identities = 202/202 (100%), Positives = 202/202 (100%) Query: 1 MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDS 60 MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDS Sbjct: 1 MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDS 60 Query: 61 GSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTR 120 GSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTR Sbjct: 61 GSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTR 120 Query: 121 YFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPL 180 YFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPL Sbjct: 121 YFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPL 180 Query: 181 CDRNRATMLKDENDKVIAIKVV 202 CDRNRATMLKDENDKVIAIKVV Sbjct: 181 CDRNRATMLKDENDKVIAIKVV 202 >3CUQ-C nrPDB Length = 176 Score = 256 bits (654), Expect = 4e-69, Method: Composition-based stats. Identities = 56/185 (30%), Positives = 91/185 (49%), Gaps = 27/185 (14%) Query: 5 PPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDSGSTD 64 P Y FPP +T QPN TR++Q++ W ++ +C+ K M TV+ E Sbjct: 8 PWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSM----TVMEAQE------- 56 Query: 65 NDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTRYFIL 124 LFNN +QR + I + ++ K+G +D+S + + I+ Sbjct: 57 --------SPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKS--------KSSFLIM 100 Query: 125 WKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPLCDRN 184 W+ + W LI QW SG+ N V TLYEL+ G++T + EFH + E+ L L+ L + Sbjct: 101 WRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEH 160 Query: 185 RATML 189 +A ++ Sbjct: 161 KAEII 165 >2ZME-C nrPDB Length = 102 Score = 142 bits (358), Expect = 9e-35, Method: Composition-based stats. Identities = 32/122 (26%), Positives = 54/122 (44%), Gaps = 27/122 (22%) Query: 5 PPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIWYMSVDGTVINDNELDSGSTD 64 P Y FPP +T QPN TR++Q++ W ++ +C+ K M TV+ E Sbjct: 8 PWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSM----TVMEAQE------- 56 Query: 65 NDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTRYFIL 124 LFNN +QR + I + ++ K+G +D+S + + I+ Sbjct: 57 --------SPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKS--------KSSFLIM 100 Query: 125 WK 126 W+ Sbjct: 101 WR 102 >3HTU-A nrPDB Length = 79 Score = 124 bits (312), Expect = 2e-29, Method: Composition-based stats. Identities = 24/66 (36%), Positives = 39/66 (59%) Query: 124 LWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPLCDR 183 + + + W LI QW SG+ N V TLYEL+ G++T + EFH + E+ L L+ L Sbjct: 3 MGRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQE 62 Query: 184 NRATML 189 ++A ++ Sbjct: 63 HKAEII 68 >5XOM-A nrPDB Length = 393 Score = 32.9 bits (73), Expect = 0.088, Method: Composition-based stats. Identities = 24/129 (18%), Positives = 51/129 (39%), Gaps = 20/129 (15%) Query: 83 RSVSQVFIDEIWSQMTKEGKCLPID-QSGRRSSNTTTTRYFILWKSLDSWASL------- 134 + ++ FI + + GKCL + + +SN T ILW + W L Sbjct: 170 KKLATTFIKDKDENLCFYGKCLYCNRKEPACASNVTMEGALILW-LPEKWPVLKLPHPWR 228 Query: 135 ------ILQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPLCDRNRATM 188 + +W DS V+ ++G ++ + ++ ++ L DR+ Sbjct: 229 RTYNKKMAKWETDSHYCESVVIKEPYTKGPRLLD-----LIDTSIFDFLIGNADRHHYEY 283 Query: 189 LKDENDKVI 197 +++EN ++ Sbjct: 284 IENENGSMV 292 >1SZ2-A nrPDB Length = 332 Score = 29.8 bits (65), Expect = 0.91, Method: Composition-based stats. Identities = 10/64 (15%), Positives = 24/64 (37%), Gaps = 4/64 (6%) Query: 136 LQWFEDSGKLNQVITLYELSEGDETVNWEFHRMPESLLYYCLKPLCDRNRATMLKDENDK 195 D G N + L +++ G+ + + + L ++ + ++ + D Sbjct: 15 YALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHKVEV----KDG 70 Query: 196 VIAI 199 IAI Sbjct: 71 CIAI 74 >2C4M-A nrPDB Length = 796 Score = 29.8 bits (65), Expect = 0.92, Method: Composition-based stats. Identities = 18/94 (19%), Positives = 40/94 (42%), Gaps = 7/94 (7%) Query: 76 FNNEDIQRSVSQVFIDEIWSQMTKEGKCLPIDQSGRRSSNTTTTRYFILWKSLDSWASLI 135 F++ + + + I E+ + E + S T + +L ++L+ W I Sbjct: 290 FHSVQLNDTHPVLAIPELMRLLMDEHDMGWEESWAIVSKTFAYTNHTVLTEALEQWDEQI 349 Query: 136 LQ---W--FEDSGKLNQVITLYELSEG--DETVN 162 Q W +E ++++ L ++G +ET+N Sbjct: 350 FQQLFWRVWEIIAEIDRRFRLERAADGLDEETIN 383 >1GWF-A nrPDB Length = 503 Score = 29.1 bits (63), Expect = 1.4, Method: Composition-based stats. Identities = 12/56 (21%), Positives = 22/56 (39%), Gaps = 11/56 (19%) Query: 125 WKSLDSWASLILQWFEDSGKLNQVI-------TLYELSEGDET----VNWEFHRMP 169 W+ ++ + S W G+ + V ++ LS + T N +FHR Sbjct: 194 WREMNGYGSHTYLWVNAQGEKHWVKYHFISQQGVHNLSNDEATKIAGENADFHRQD 249 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.305 0.118 0.334 Lambda K H 0.267 0.0369 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,145,624 Number of Sequences: 36641 Number of extensions: 247213 Number of successful extensions: 689 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 668 Number of HSP's gapped (non-prelim): 21 length of query: 202 length of database: 10,336,785 effective HSP length: 92 effective length of query: 110 effective length of database: 6,965,813 effective search space: 766239430 effective search space used: 766239430 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 56 (26.3 bits)