BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P47128 (113 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5J9Q-F nrPDB 229 3e-61 1XWI-A nrPDB 30 0.26 2GM8-A nrPDB 27 1.7 5AWM-A nrPDB 26 3.0 6FTX-W nrPDB 26 4.3 5J1I-A nrPDB 26 4.6 3MWY-W nrPDB 26 4.7 2ZAO-A nrPDB 25 5.0 1TZ7-A nrPDB 25 8.7 >5J9Q-F nrPDB Length = 113 Score = 229 bits (583), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 113/113 (100%), Positives = 113/113 (100%) Query: 1 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHG 60 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHG Sbjct: 1 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHG 60 Query: 61 SKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND 113 SKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND Sbjct: 61 SKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND 113 >1XWI-A nrPDB Length = 322 Score = 30.0 bits (66), Expect = 0.26, Method: Compositional matrix adjust. Identities = 15/52 (28%), Positives = 28/52 (53%) Query: 60 GSKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQ 111 G ++ + G ++ G KS+ + A + NN FS+SS+ V + G+S+ Sbjct: 40 GKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESE 91 >2GM8-A nrPDB Length = 221 Score = 27.3 bits (59), Expect = 1.7, Method: Compositional matrix adjust. Identities = 16/41 (39%), Positives = 22/41 (53%) Query: 1 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKET 41 +T E+ +YEAL E+ SL+D E E N+ Y K T Sbjct: 87 VTGEMANYEALLKEVGLSLRDAAEAEPNRVNVSYMAYLKST 127 >5AWM-A nrPDB Length = 394 Score = 26.2 bits (56), Expect = 3.0, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 19/27 (70%) Query: 24 EQEDTFDNLQQEIYDKETEYFSHNSNN 50 E+E T + ++ IY++ +Y +HN+NN Sbjct: 365 EREHTVEQWKELIYEEVMDYEAHNTNN 391 >6FTX-W nrPDB Length = 878 Score = 25.8 bits (55), Expect = 4.3, Method: Compositional matrix adjust. Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 8/57 (14%) Query: 11 LKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGH-GGAHGSKSHYS 66 LK +++KSL + E+ L+ E+ D +TEY+ + N+S GA G H+S Sbjct: 407 LKKDVEKSLPSKTER-----ILRVELSDVQTEYYKNILTKNYSALTAGAKG--GHFS 456 >5J1I-A nrPDB Length = 373 Score = 25.8 bits (55), Expect = 4.6, Method: Composition-based stats. Identities = 14/32 (43%), Positives = 17/32 (53%) Query: 5 LKSYEALKAELKKSLQDRREQEDTFDNLQQEI 36 L EA KA LKK Q+ TFD L+ E+ Sbjct: 150 LPELEATKASLKKLRAQAEAQQPTFDALRDEL 181 >3MWY-W nrPDB Length = 800 Score = 25.8 bits (55), Expect = 4.7, Method: Compositional matrix adjust. Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 8/57 (14%) Query: 11 LKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGH-GGAHGSKSHYS 66 LK +++KSL + E+ L+ E+ D +TEY+ + N+S GA G H+S Sbjct: 459 LKKDVEKSLPSKTER-----ILRVELSDVQTEYYKNILTKNYSALTAGAKG--GHFS 508 >2ZAO-A nrPDB Length = 444 Score = 25.4 bits (54), Expect = 5.0, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 28/52 (53%) Query: 60 GSKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQ 111 G ++ + G ++ G KS+ + A + NN FS+SS+ V + G+S+ Sbjct: 162 GKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESE 213 >1TZ7-A nrPDB Length = 505 Score = 24.6 bits (52), Expect = 8.7, Method: Composition-based stats. Identities = 9/23 (39%), Positives = 13/23 (56%) Query: 37 YDKETEYFSHNSNNNHSGHGGAH 59 YDKE+E+ HN N+ + H Sbjct: 382 YDKESEHLPHNVEENNVYYTSTH 404 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5J9Q-F nrPDB 167 1e-42 Sequences not found previously or not previously below threshold: 5AWM-A nrPDB 29 0.58 2GM8-A nrPDB 29 0.58 5J1I-A nrPDB 28 0.87 5J1G-A nrPDB 28 0.87 6FTX-W nrPDB 28 1.1 3MWY-W nrPDB 28 1.1 2ZAO-A nrPDB 28 1.1 1XWI-A nrPDB 28 1.1 1XOC-A nrPDB 27 1.6 3C7F-A nrPDB 27 2.3 5LJ5-D nrPDB 26 5.9 1Q67-A nrPDB 25 7.5 CONVERGED! >5J9Q-F nrPDB Length = 113 Score = 167 bits (422), Expect = 1e-42, Method: Composition-based stats. Identities = 113/113 (100%), Positives = 113/113 (100%) Query: 1 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHG 60 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHG Sbjct: 1 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHG 60 Query: 61 SKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND 113 SKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND Sbjct: 61 SKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND 113 >5AWM-A nrPDB Length = 394 Score = 28.6 bits (62), Expect = 0.58, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 19/27 (70%) Query: 24 EQEDTFDNLQQEIYDKETEYFSHNSNN 50 E+E T + ++ IY++ +Y +HN+NN Sbjct: 365 EREHTVEQWKELIYEEVMDYEAHNTNN 391 >2GM8-A nrPDB Length = 221 Score = 28.6 bits (62), Expect = 0.58, Method: Composition-based stats. Identities = 16/41 (39%), Positives = 22/41 (53%) Query: 1 MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKET 41 +T E+ +YEAL E+ SL+D E E N+ Y K T Sbjct: 87 VTGEMANYEALLKEVGLSLRDAAEAEPNRVNVSYMAYLKST 127 >5J1I-A nrPDB Length = 373 Score = 28.2 bits (61), Expect = 0.87, Method: Composition-based stats. Identities = 14/32 (43%), Positives = 17/32 (53%) Query: 5 LKSYEALKAELKKSLQDRREQEDTFDNLQQEI 36 L EA KA LKK Q+ TFD L+ E+ Sbjct: 150 LPELEATKASLKKLRAQAEAQQPTFDALRDEL 181 >5J1G-A nrPDB Length = 234 Score = 28.2 bits (61), Expect = 0.87, Method: Composition-based stats. Identities = 14/32 (43%), Positives = 17/32 (53%) Query: 5 LKSYEALKAELKKSLQDRREQEDTFDNLQQEI 36 L EA KA LKK Q+ TFD L+ E+ Sbjct: 150 LPELEATKASLKKLRAQAEAQQPTFDALRDEL 181 >6FTX-W nrPDB Length = 878 Score = 27.8 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 8/57 (14%) Query: 11 LKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSG-HGGAHGSKSHYS 66 LK +++KSL + E+ L+ E+ D +TEY+ + N+S GA G H+S Sbjct: 407 LKKDVEKSLPSKTERI-----LRVELSDVQTEYYKNILTKNYSALTAGAKG--GHFS 456 >3MWY-W nrPDB Length = 800 Score = 27.8 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 8/57 (14%) Query: 11 LKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSG-HGGAHGSKSHYS 66 LK +++KSL + E+ L+ E+ D +TEY+ + N+S GA G H+S Sbjct: 459 LKKDVEKSLPSKTERI-----LRVELSDVQTEYYKNILTKNYSALTAGAKG--GHFS 508 >2ZAO-A nrPDB Length = 444 Score = 27.8 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 28/52 (53%) Query: 60 GSKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQ 111 G ++ + G ++ G KS+ + A + NN FS+SS+ V + G+S+ Sbjct: 162 GKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESE 213 >1XWI-A nrPDB Length = 322 Score = 27.8 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 28/52 (53%) Query: 60 GSKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQ 111 G ++ + G ++ G KS+ + A + NN FS+SS+ V + G+S+ Sbjct: 40 GKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESE 91 >1XOC-A nrPDB Length = 520 Score = 27.5 bits (59), Expect = 1.6, Method: Composition-based stats. Identities = 12/48 (25%), Positives = 24/48 (50%) Query: 17 KSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHGSKSH 64 KS+ DR++ ++ + Q+I + + F + NN+ + G K H Sbjct: 458 KSISDRKQYSKEYEQIYQKIAEDQPYTFLYYPNNHMAMPENLEGYKYH 505 >3C7F-A nrPDB Length = 487 Score = 26.7 bits (57), Expect = 2.3, Method: Composition-based stats. Identities = 21/66 (31%), Positives = 31/66 (46%), Gaps = 4/66 (6%) Query: 47 NSNNNHSGHGGAHGSKSHYSGNI---IKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYV 103 NSN H GA Y+G + + D S+ + D++F N +R+F +SSA V Sbjct: 12 NSNPLIDHHLGADPVALTYNGRVYIYMSSDDYEYNSNGTIKDNSFANLNRVFVISSADMV 71 Query: 104 K-QQHG 108 HG Sbjct: 72 NWTDHG 77 >5LJ5-D nrPDB Length = 278 Score = 25.5 bits (54), Expect = 5.9, Method: Composition-based stats. Identities = 10/36 (27%), Positives = 18/36 (50%) Query: 16 KKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNN 51 K + Q++ D DNL +++D + + NNN Sbjct: 204 KHAQQEKAVTTDDLDNLVDQVFDNHRQRTNKPGNNN 239 >1Q67-A nrPDB Length = 231 Score = 25.1 bits (53), Expect = 7.5, Method: Composition-based stats. Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 4/57 (7%) Query: 24 EQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHGSK---SHYSGNIIKGFDTFS 77 ++ D FD+ Q + + S NSN N SG+G ++ S ++ G + G D ++ Sbjct: 84 QEVDIFDS-QNGSNNIQVNNGSDNSNRNSSGNGNSYKSNDSLTYNCGKTLSGKDIYN 139 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.123 0.343 Lambda K H 0.267 0.0388 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,709,594 Number of Sequences: 36641 Number of extensions: 176901 Number of successful extensions: 486 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 32 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 448 Number of HSP's gapped (non-prelim): 55 length of query: 113 length of database: 10,336,785 effective HSP length: 79 effective length of query: 34 effective length of database: 7,442,146 effective search space: 253032964 effective search space used: 253032964 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 52 (24.7 bits)