BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P47112 (604 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5V8F-8 nrPDB 1251 0.0 2NWB-A nrPDB 29 4.5 1RZ1-A nrPDB 29 5.0 3VH2-A nrPDB 28 8.4 >5V8F-8 nrPDB Length = 604 Score = 1251 bits (3238), Expect = 0.0, Method: Compositional matrix adjust. Identities = 604/604 (100%), Positives = 604/604 (100%) Query: 1 MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFLLKNYANKAVLDALLAG 60 MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFLLKNYANKAVLDALLAG Sbjct: 1 MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFLLKNYANKAVLDALLAG 60 Query: 61 LTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFK 120 LTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFK Sbjct: 61 LTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFK 120 Query: 121 LGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNGSTHDGADAGATVLPTGDDFQ 180 LGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNGSTHDGADAGATVLPTGDDFQ Sbjct: 121 LGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNGSTHDGADAGATVLPTGDDFQ 180 Query: 181 YLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARWS 240 YLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARWS Sbjct: 181 YLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARWS 240 Query: 241 QGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEGTIANHLLEQQIKAFPKVAQQYYPR 300 QGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEGTIANHLLEQQIKAFPKVAQQYYPR Sbjct: 241 QGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEGTIANHLLEQQIKAFPKVAQQYYPR 300 Query: 301 ELSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPELHVLLPQISSMMKRKIVQDDI 360 ELSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPELHVLLPQISSMMKRKIVQDDI Sbjct: 301 ELSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPELHVLLPQISSMMKRKIVQDDI 360 Query: 361 LKLLTIWSDAYVVELNSRGELTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASKDE 420 LKLLTIWSDAYVVELNSRGELTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASKDE Sbjct: 361 LKLLTIWSDAYVVELNSRGELTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASKDE 420 Query: 421 IMTDVPAFKINKRRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQ 480 IMTDVPAFKINKRRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQ Sbjct: 421 IMTDVPAFKINKRRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQ 480 Query: 481 ANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVD 540 ANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVD Sbjct: 481 ANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVD 540 Query: 541 SLQDSNNPIGTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKS 600 SLQDSNNPIGTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKS Sbjct: 541 SLQDSNNPIGTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKS 600 Query: 601 KQQD 604 KQQD Sbjct: 601 KQQD 604 >2NWB-A nrPDB Length = 400 Score = 29.3 bits (64), Expect = 4.5, Method: Compositional matrix adjust. Identities = 25/94 (26%), Positives = 45/94 (47%), Gaps = 15/94 (15%) Query: 273 KGEGTIANHLLEQQIKAFPKVAQQYYPRELSILRLQDAMKFVKELFT--VCETVLSLNA- 329 + +G I ++ +K F +V + L Q A++ EL T + E +S+ Sbjct: 293 QAKGCIHQDWYQENLKLFIEVCE---------LHGQTAIQHHNELVTKYIAEPSVSMEQQ 343 Query: 330 -LSRSTGVPPELHVLLPQISSMMKRK--IVQDDI 360 L++ T P LHVLL + + R+ +++DDI Sbjct: 344 HLAKVTASGPPLHVLLASLERLRDRRAAVLRDDI 377 >1RZ1-A nrPDB Length = 161 Score = 29.3 bits (64), Expect = 5.0, Method: Composition-based stats. Identities = 16/81 (19%), Positives = 43/81 (53%), Gaps = 3/81 (3%) Query: 480 QANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSL-- 537 +A +++++++ ++ + +LSQ QK A ++ + D+ F G P + L+ + Sbjct: 53 KAKMLEKIQQSKKYAVNILSQDQKVLSMNFAGQLEKPVDVQFEELGGLPVIKDALAQISC 112 Query: 538 -IVDSLQDSNNPIGTKEASEI 557 +V+ +Q ++ + E ++I Sbjct: 113 QVVNEVQAGDHTLFIGEVTDI 133 >3VH2-A nrPDB Length = 616 Score = 28.5 bits (62), Expect = 8.4, Method: Compositional matrix adjust. Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 3/66 (4%) Query: 13 LRVPVIDLNRVSDEEQL--LPVVRAILLQHDT-FLLKNYANKAVLDALLAGLTTKDLPDT 69 L +P+I V++E Q +RA++ +HD FLL + L +LL+ + K + + Sbjct: 404 LSIPMIGHKLVNEEAQHKDFDRLRALIKEHDIIFLLVDSRESRWLPSLLSNIENKTVINA 463 Query: 70 SQGFDA 75 + GFD+ Sbjct: 464 ALGFDS 469 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5V8F-8 nrPDB 1267 0.0 Sequences not found previously or not previously below threshold: 3CUE-B nrPDB 30 2.1 1RZ1-A nrPDB 30 2.9 2NWB-A nrPDB 30 3.1 3VH2-A nrPDB 30 3.6 3T7E-A nrPDB 30 3.6 CONVERGED! >5V8F-8 nrPDB Length = 604 Score = 1267 bits (3279), Expect = 0.0, Method: Composition-based stats. Identities = 604/604 (100%), Positives = 604/604 (100%) Query: 1 MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFLLKNYANKAVLDALLAG 60 MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFLLKNYANKAVLDALLAG Sbjct: 1 MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFLLKNYANKAVLDALLAG 60 Query: 61 LTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFK 120 LTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFK Sbjct: 61 LTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFK 120 Query: 121 LGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNGSTHDGADAGATVLPTGDDFQ 180 LGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNGSTHDGADAGATVLPTGDDFQ Sbjct: 121 LGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNGSTHDGADAGATVLPTGDDFQ 180 Query: 181 YLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARWS 240 YLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARWS Sbjct: 181 YLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARWS 240 Query: 241 QGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEGTIANHLLEQQIKAFPKVAQQYYPR 300 QGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEGTIANHLLEQQIKAFPKVAQQYYPR Sbjct: 241 QGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEGTIANHLLEQQIKAFPKVAQQYYPR 300 Query: 301 ELSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPELHVLLPQISSMMKRKIVQDDI 360 ELSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPELHVLLPQISSMMKRKIVQDDI Sbjct: 301 ELSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPELHVLLPQISSMMKRKIVQDDI 360 Query: 361 LKLLTIWSDAYVVELNSRGELTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASKDE 420 LKLLTIWSDAYVVELNSRGELTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASKDE Sbjct: 361 LKLLTIWSDAYVVELNSRGELTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASKDE 420 Query: 421 IMTDVPAFKINKRRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQ 480 IMTDVPAFKINKRRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQ Sbjct: 421 IMTDVPAFKINKRRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQ 480 Query: 481 ANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVD 540 ANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVD Sbjct: 481 ANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVD 540 Query: 541 SLQDSNNPIGTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKS 600 SLQDSNNPIGTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKS Sbjct: 541 SLQDSNNPIGTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKS 600 Query: 601 KQQD 604 KQQD Sbjct: 601 KQQD 604 >3CUE-B nrPDB Length = 283 Score = 30.4 bits (67), Expect = 2.1, Method: Composition-based stats. Identities = 17/59 (28%), Positives = 31/59 (52%) Query: 433 RRSSSNSKTVLSSKVQTKSSNANALNNSRYLANSKENFMYKEKMPDSQANLMDRLRERE 491 R S+ S+ SSK+ S++ L NS ++ N +EK +S +N + ++R R+ Sbjct: 115 RASAFLSQNESSSKLSNASNSPGMLANSSTATSASANERLQEKQTESLSNYITKMRRRD 173 >1RZ1-A nrPDB Length = 161 Score = 30.1 bits (66), Expect = 2.9, Method: Composition-based stats. Identities = 16/81 (19%), Positives = 43/81 (53%), Gaps = 3/81 (3%) Query: 480 QANLMDRLRERERRSAALLSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSL-- 537 +A +++++++ ++ + +LSQ QK A ++ + D+ F G P + L+ + Sbjct: 53 KAKMLEKIQQSKKYAVNILSQDQKVLSMNFAGQLEKPVDVQFEELGGLPVIKDALAQISC 112 Query: 538 -IVDSLQDSNNPIGTKEASEI 557 +V+ +Q ++ + E ++I Sbjct: 113 QVVNEVQAGDHTLFIGEVTDI 133 >2NWB-A nrPDB Length = 400 Score = 30.1 bits (66), Expect = 3.1, Method: Composition-based stats. Identities = 25/92 (27%), Positives = 46/92 (50%), Gaps = 12/92 (13%) Query: 281 HLLEQQIKAFPKVAQQYYPRELSI------LRLQDAMKFVKELFT--VCETVLSLNA--L 330 +L++ ++A + Q +Y L + L Q A++ EL T + E +S+ L Sbjct: 286 NLMDDFLQAKGCIHQDWYQENLKLFIEVCELHGQTAIQHHNELVTKYIAEPSVSMEQQHL 345 Query: 331 SRSTGVPPELHVLLPQISSMMKRK--IVQDDI 360 ++ T P LHVLL + + R+ +++DDI Sbjct: 346 AKVTASGPPLHVLLASLERLRDRRAAVLRDDI 377 >3VH2-A nrPDB Length = 616 Score = 29.7 bits (65), Expect = 3.6, Method: Composition-based stats. Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 3/66 (4%) Query: 13 LRVPVIDLNRVSDEEQL--LPVVRAILLQHDT-FLLKNYANKAVLDALLAGLTTKDLPDT 69 L +P+I V++E Q +RA++ +HD FLL + L +LL+ + K + + Sbjct: 404 LSIPMIGHKLVNEEAQHKDFDRLRALIKEHDIIFLLVDSRESRWLPSLLSNIENKTVINA 463 Query: 70 SQGFDA 75 + GFD+ Sbjct: 464 ALGFDS 469 >3T7E-A nrPDB Length = 344 Score = 29.7 bits (65), Expect = 3.6, Method: Composition-based stats. Identities = 20/66 (30%), Positives = 36/66 (54%), Gaps = 3/66 (4%) Query: 13 LRVPVIDLNRVSDEEQL--LPVVRAILLQHDT-FLLKNYANKAVLDALLAGLTTKDLPDT 69 L +P+I V++E Q +RA++ +HD FLL + L +LL+ + K + + Sbjct: 115 LSIPMIGHKLVNEEAQHKDFDRLRALIKEHDIIFLLVDSRESRWLPSLLSNIENKTVINA 174 Query: 70 SQGFDA 75 + GFD+ Sbjct: 175 ALGFDS 180 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.132 0.379 Lambda K H 0.267 0.0406 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 22,928,018 Number of Sequences: 36641 Number of extensions: 923112 Number of successful extensions: 2204 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 2191 Number of HSP's gapped (non-prelim): 24 length of query: 604 length of database: 10,336,785 effective HSP length: 103 effective length of query: 501 effective length of database: 6,562,762 effective search space: 3287943762 effective search space used: 3287943762 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 62 (28.5 bits)