BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P47076
         (161 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

6CNB-D nrPDB                                                          330   2e-91
3AYH-A nrPDB                                                           45   9e-06
3WEN-A nrPDB                                                           30   0.61 
1W8X-P nrPDB                                                           27   3.0  
6GAW-Ao nrPDB                                                          26   6.5  
4O9I-X nrPDB                                                           26   9.7  

>6CNB-D nrPDB
          Length = 161

 Score =  330 bits (845), Expect = 2e-91,   Method: Compositional matrix adjust.
 Identities = 161/161 (100%), Positives = 161/161 (100%)

Query: 1   MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
           MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR
Sbjct: 1   MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60

Query: 61  NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
           NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK
Sbjct: 61  NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120

Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161
           LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA
Sbjct: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161


>3AYH-A nrPDB
          Length = 136

 Score = 45.4 bits (106), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 44/158 (27%), Positives = 73/158 (46%), Gaps = 33/158 (20%)

Query: 1   MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
           MKVLE R+A+L++ EV   L ++E +         A  + R   +     N   L+ I  
Sbjct: 8   MKVLEARDAYLTNAEVFFHLKEMENEQ-------NARTQERGAAQALVCEN---LRTIQF 57

Query: 61  NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
            ++ YLS   N      EG                  ++ E F + +   N F+L KAE 
Sbjct: 58  EILKYLSSQGNC-----EG------------------LTKERFLDCIAIFNEFELTKAEI 94

Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIIS 158
           L I+N  P+++  LY+ +E  + RF E+ I +++E I+
Sbjct: 95  LVILNNKPSSVPELYACIEGIEERFKEEDIFKLVEKIN 132


>3WEN-A nrPDB
          Length = 913

 Score = 29.6 bits (65), Expect = 0.61,   Method: Composition-based stats.
 Identities = 12/34 (35%), Positives = 19/34 (55%)

Query: 112 SFKLFKAEKLQIVNQLPANMVHLYSIVEECDARF 145
           +F ++K + LQ+ + LPA   HLY + E     F
Sbjct: 185 TFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKPTF 218


>1W8X-P nrPDB
          Length = 117

 Score = 27.3 bits (59), Expect = 3.0,   Method: Compositional matrix adjust.
 Identities = 20/80 (25%), Positives = 34/80 (42%), Gaps = 1/80 (1%)

Query: 28  LWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVNYLSINKNFINQEDEGEERESSGA 87
           LW +   AA   S  +G     YN P+   +T  V  Y S +    N+        +  A
Sbjct: 21  LWFRNRPAAQVASNWEGPPYMTYNQPQAGSVTLPVAGYTSPSPTLPNRNRSCGCNPAVSA 80

Query: 88  KDAEKSGI-SKMSDESFAEL 106
             A+ + + SK++D   ++L
Sbjct: 81  AMAQGADLASKLTDSITSQL 100


>6GAW-Ao nrPDB
          Length = 692

 Score = 26.2 bits (56), Expect = 6.5,   Method: Compositional matrix adjust.
 Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 12/100 (12%)

Query: 67  SINKNFINQEDEGEERESSGAKDAEKS--------GIS---KMSDESFAELMTKLNSFKL 115
           S N NF  Q ++ EE E +   D EKS        G++   K + E    LM + N+   
Sbjct: 204 SANYNF-QQREQSEELEEATEADNEKSKTKAGHHLGVTWRTKNNAERIFALMPEKNAHSY 262

Query: 116 FKAEKLQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLE 155
               +  + +Q P   ++LY+++     R D  T   ++E
Sbjct: 263 CTMIRGMVKHQAPTQALNLYTVLLNNRLRADVYTFNSLIE 302


>4O9I-X nrPDB
          Length = 202

 Score = 25.8 bits (55), Expect = 9.7,   Method: Compositional matrix adjust.
 Identities = 20/85 (23%), Positives = 38/85 (44%), Gaps = 4/85 (4%)

Query: 70  KNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEKL---QIVNQ 126
           +N+  + D  E        D EKS   K  D  FAE+  +   + + K E +   +I+N 
Sbjct: 95  RNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGI-KPEWMMIHRILNH 153

Query: 127 LPANMVHLYSIVEECDARFDEKTIE 151
                 H++ +++  D  +D+ + E
Sbjct: 154 SVDKKGHVHYLIKWRDLPYDQASWE 178


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

6CNB-D nrPDB                                                          227   2e-60
3AYH-A nrPDB                                                          162   7e-41

Sequences not found previously or not previously below threshold:

2LD7-B nrPDB                                                           32   0.13 
5CHC-A nrPDB                                                           31   0.21 
1ZDL-A nrPDB                                                           28   1.4  
6J79-A nrPDB                                                           28   2.2  
4HKM-A nrPDB                                                           26   7.7  


CONVERGED!
>6CNB-D nrPDB
          Length = 161

 Score =  227 bits (577), Expect = 2e-60,   Method: Composition-based stats.
 Identities = 161/161 (100%), Positives = 161/161 (100%)

Query: 1   MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
           MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR
Sbjct: 1   MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60

Query: 61  NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
           NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK
Sbjct: 61  NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120

Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161
           LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA
Sbjct: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161


>3AYH-A nrPDB
          Length = 136

 Score =  162 bits (409), Expect = 7e-41,   Method: Composition-based stats.
 Identities = 42/158 (26%), Positives = 71/158 (44%), Gaps = 33/158 (20%)

Query: 1   MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
           MKVLE R+A+L++ EV   L ++E +         A  + R   +     N   L+ I  
Sbjct: 8   MKVLEARDAYLTNAEVFFHLKEMENEQ-------NARTQERGAAQALVCEN---LRTIQF 57

Query: 61  NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
            ++ YLS   N                          ++ E F + +   N F+L KAE 
Sbjct: 58  EILKYLSSQGNC-----------------------EGLTKERFLDCIAIFNEFELTKAEI 94

Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIIS 158
           L I+N  P+++  LY+ +E  + RF E+ I +++E I+
Sbjct: 95  LVILNNKPSSVPELYACIEGIEERFKEEDIFKLVEKIN 132


>2LD7-B nrPDB
          Length = 75

 Score = 31.8 bits (70), Expect = 0.13,   Method: Composition-based stats.
 Identities = 14/62 (22%), Positives = 24/62 (38%)

Query: 74  NQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEKLQIVNQLPANMVH 133
           N    G   ES       K+  S  + E+F   +   N   + +AE +Q+V+        
Sbjct: 2   NASKHGVGTESLFFDKVRKALRSAEAYENFLRCLVIFNQEVISRAELVQLVSPFLGKFPE 61

Query: 134 LY 135
           L+
Sbjct: 62  LF 63


>5CHC-A nrPDB
          Length = 899

 Score = 31.0 bits (68), Expect = 0.21,   Method: Composition-based stats.
 Identities = 13/52 (25%), Positives = 23/52 (44%), Gaps = 2/52 (3%)

Query: 13  DYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVN 64
           + E  K++ DL    LW+Q ++           Q    N P+ +GIT  ++ 
Sbjct: 639 NDEKFKWVRDLSN--LWNQMTMDGKLAEDEAAAQYILDNAPQSKGITIQMLR 688


>1ZDL-A nrPDB
          Length = 517

 Score = 28.3 bits (61), Expect = 1.4,   Method: Composition-based stats.
 Identities = 19/90 (21%), Positives = 38/90 (42%), Gaps = 1/90 (1%)

Query: 55  LQGITRNVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFK 114
           L+G   + +  L  N+  +  ED    +E +G  D     I ++ +     L  K     
Sbjct: 269 LKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNL-EKAGIST 327

Query: 115 LFKAEKLQIVNQLPANMVHLYSIVEECDAR 144
             K +K+ +  Q   ++ H+Y+I +  + R
Sbjct: 328 NPKNQKIIVDAQEATSVPHIYAIGDVAEGR 357


>6J79-A nrPDB
          Length = 871

 Score = 27.9 bits (60), Expect = 2.2,   Method: Composition-based stats.
 Identities = 21/97 (21%), Positives = 39/97 (40%), Gaps = 8/97 (8%)

Query: 5   EERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVN 64
           E + AFL + E+ + L  L  +   DQ   A+     +           +++   RN++ 
Sbjct: 222 EAKTAFLLNIELFEELQALLPEEHKDQS--ASQTMQTTAPPVKESSFVEKMKKTGRNIIV 279

Query: 65  YLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDE 101
           +         Q    EE  +  +KDA + G+  MS +
Sbjct: 280 FYGS------QTGTAEEFANRLSKDAHRYGMRGMSAD 310


>4HKM-A nrPDB
          Length = 346

 Score = 26.0 bits (55), Expect = 7.7,   Method: Composition-based stats.
 Identities = 19/97 (19%), Positives = 40/97 (41%), Gaps = 11/97 (11%)

Query: 52  HPELQGITRNVVNYLSINKNFINQEDEGEERESSGAKD------AEKSGISKMSDESFAE 105
           HP+L GI   V+  L   +  +    +G +  S GA          +    ++  E F  
Sbjct: 201 HPDLVGIQARVLQELGAERALVVWGRDGMDELSLGAGTLVGELRDGQVHEYEVHPEDFGI 260

Query: 106 LMTKLNSFKL-----FKAEKLQIVNQLPANMVHLYSI 137
            M+   + K+      +A  LQ+++ +P   + + ++
Sbjct: 261 AMSASRNLKVADAAESRAMLLQVLDNVPGPALDIVAL 297


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.308    0.118    0.282 

Lambda     K      H
   0.267   0.0351    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,663,739
Number of Sequences: 36641
Number of extensions: 150183
Number of successful extensions: 382
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 362
Number of HSP's gapped (non-prelim): 41
length of query: 161
length of database: 10,336,785
effective HSP length: 89
effective length of query: 72
effective length of database: 7,075,736
effective search space: 509452992
effective search space used: 509452992
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 54 (25.6 bits)