BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= P47076
(161 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
6CNB-D nrPDB 330 2e-91
3AYH-A nrPDB 45 9e-06
3WEN-A nrPDB 30 0.61
1W8X-P nrPDB 27 3.0
6GAW-Ao nrPDB 26 6.5
4O9I-X nrPDB 26 9.7
>6CNB-D nrPDB
Length = 161
Score = 330 bits (845), Expect = 2e-91, Method: Compositional matrix adjust.
Identities = 161/161 (100%), Positives = 161/161 (100%)
Query: 1 MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR
Sbjct: 1 MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
Query: 61 NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK
Sbjct: 61 NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161
LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA
Sbjct: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161
>3AYH-A nrPDB
Length = 136
Score = 45.4 bits (106), Expect = 9e-06, Method: Compositional matrix adjust.
Identities = 44/158 (27%), Positives = 73/158 (46%), Gaps = 33/158 (20%)
Query: 1 MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
MKVLE R+A+L++ EV L ++E + A + R + N L+ I
Sbjct: 8 MKVLEARDAYLTNAEVFFHLKEMENEQ-------NARTQERGAAQALVCEN---LRTIQF 57
Query: 61 NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
++ YLS N EG ++ E F + + N F+L KAE
Sbjct: 58 EILKYLSSQGNC-----EG------------------LTKERFLDCIAIFNEFELTKAEI 94
Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIIS 158
L I+N P+++ LY+ +E + RF E+ I +++E I+
Sbjct: 95 LVILNNKPSSVPELYACIEGIEERFKEEDIFKLVEKIN 132
>3WEN-A nrPDB
Length = 913
Score = 29.6 bits (65), Expect = 0.61, Method: Composition-based stats.
Identities = 12/34 (35%), Positives = 19/34 (55%)
Query: 112 SFKLFKAEKLQIVNQLPANMVHLYSIVEECDARF 145
+F ++K + LQ+ + LPA HLY + E F
Sbjct: 185 TFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKPTF 218
>1W8X-P nrPDB
Length = 117
Score = 27.3 bits (59), Expect = 3.0, Method: Compositional matrix adjust.
Identities = 20/80 (25%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Query: 28 LWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVNYLSINKNFINQEDEGEERESSGA 87
LW + AA S +G YN P+ +T V Y S + N+ + A
Sbjct: 21 LWFRNRPAAQVASNWEGPPYMTYNQPQAGSVTLPVAGYTSPSPTLPNRNRSCGCNPAVSA 80
Query: 88 KDAEKSGI-SKMSDESFAEL 106
A+ + + SK++D ++L
Sbjct: 81 AMAQGADLASKLTDSITSQL 100
>6GAW-Ao nrPDB
Length = 692
Score = 26.2 bits (56), Expect = 6.5, Method: Compositional matrix adjust.
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 12/100 (12%)
Query: 67 SINKNFINQEDEGEERESSGAKDAEKS--------GIS---KMSDESFAELMTKLNSFKL 115
S N NF Q ++ EE E + D EKS G++ K + E LM + N+
Sbjct: 204 SANYNF-QQREQSEELEEATEADNEKSKTKAGHHLGVTWRTKNNAERIFALMPEKNAHSY 262
Query: 116 FKAEKLQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLE 155
+ + +Q P ++LY+++ R D T ++E
Sbjct: 263 CTMIRGMVKHQAPTQALNLYTVLLNNRLRADVYTFNSLIE 302
>4O9I-X nrPDB
Length = 202
Score = 25.8 bits (55), Expect = 9.7, Method: Compositional matrix adjust.
Identities = 20/85 (23%), Positives = 38/85 (44%), Gaps = 4/85 (4%)
Query: 70 KNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEKL---QIVNQ 126
+N+ + D E D EKS K D FAE+ + + + K E + +I+N
Sbjct: 95 RNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGI-KPEWMMIHRILNH 153
Query: 127 LPANMVHLYSIVEECDARFDEKTIE 151
H++ +++ D +D+ + E
Sbjct: 154 SVDKKGHVHYLIKWRDLPYDQASWE 178
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
6CNB-D nrPDB 227 2e-60
3AYH-A nrPDB 162 7e-41
Sequences not found previously or not previously below threshold:
2LD7-B nrPDB 32 0.13
5CHC-A nrPDB 31 0.21
1ZDL-A nrPDB 28 1.4
6J79-A nrPDB 28 2.2
4HKM-A nrPDB 26 7.7
CONVERGED!
>6CNB-D nrPDB
Length = 161
Score = 227 bits (577), Expect = 2e-60, Method: Composition-based stats.
Identities = 161/161 (100%), Positives = 161/161 (100%)
Query: 1 MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR
Sbjct: 1 MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
Query: 61 NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK
Sbjct: 61 NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161
LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA
Sbjct: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA 161
>3AYH-A nrPDB
Length = 136
Score = 162 bits (409), Expect = 7e-41, Method: Composition-based stats.
Identities = 42/158 (26%), Positives = 71/158 (44%), Gaps = 33/158 (20%)
Query: 1 MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITR 60
MKVLE R+A+L++ EV L ++E + A + R + N L+ I
Sbjct: 8 MKVLEARDAYLTNAEVFFHLKEMENEQ-------NARTQERGAAQALVCEN---LRTIQF 57
Query: 61 NVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEK 120
++ YLS N ++ E F + + N F+L KAE
Sbjct: 58 EILKYLSSQGNC-----------------------EGLTKERFLDCIAIFNEFELTKAEI 94
Query: 121 LQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIIS 158
L I+N P+++ LY+ +E + RF E+ I +++E I+
Sbjct: 95 LVILNNKPSSVPELYACIEGIEERFKEEDIFKLVEKIN 132
>2LD7-B nrPDB
Length = 75
Score = 31.8 bits (70), Expect = 0.13, Method: Composition-based stats.
Identities = 14/62 (22%), Positives = 24/62 (38%)
Query: 74 NQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEKLQIVNQLPANMVH 133
N G ES K+ S + E+F + N + +AE +Q+V+
Sbjct: 2 NASKHGVGTESLFFDKVRKALRSAEAYENFLRCLVIFNQEVISRAELVQLVSPFLGKFPE 61
Query: 134 LY 135
L+
Sbjct: 62 LF 63
>5CHC-A nrPDB
Length = 899
Score = 31.0 bits (68), Expect = 0.21, Method: Composition-based stats.
Identities = 13/52 (25%), Positives = 23/52 (44%), Gaps = 2/52 (3%)
Query: 13 DYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVN 64
+ E K++ DL LW+Q ++ Q N P+ +GIT ++
Sbjct: 639 NDEKFKWVRDLSN--LWNQMTMDGKLAEDEAAAQYILDNAPQSKGITIQMLR 688
>1ZDL-A nrPDB
Length = 517
Score = 28.3 bits (61), Expect = 1.4, Method: Composition-based stats.
Identities = 19/90 (21%), Positives = 38/90 (42%), Gaps = 1/90 (1%)
Query: 55 LQGITRNVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFK 114
L+G + + L N+ + ED +E +G D I ++ + L K
Sbjct: 269 LKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNL-EKAGIST 327
Query: 115 LFKAEKLQIVNQLPANMVHLYSIVEECDAR 144
K +K+ + Q ++ H+Y+I + + R
Sbjct: 328 NPKNQKIIVDAQEATSVPHIYAIGDVAEGR 357
>6J79-A nrPDB
Length = 871
Score = 27.9 bits (60), Expect = 2.2, Method: Composition-based stats.
Identities = 21/97 (21%), Positives = 39/97 (40%), Gaps = 8/97 (8%)
Query: 5 EERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVN 64
E + AFL + E+ + L L + DQ A+ + +++ RN++
Sbjct: 222 EAKTAFLLNIELFEELQALLPEEHKDQS--ASQTMQTTAPPVKESSFVEKMKKTGRNIIV 279
Query: 65 YLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDE 101
+ Q EE + +KDA + G+ MS +
Sbjct: 280 FYGS------QTGTAEEFANRLSKDAHRYGMRGMSAD 310
>4HKM-A nrPDB
Length = 346
Score = 26.0 bits (55), Expect = 7.7, Method: Composition-based stats.
Identities = 19/97 (19%), Positives = 40/97 (41%), Gaps = 11/97 (11%)
Query: 52 HPELQGITRNVVNYLSINKNFINQEDEGEERESSGAKD------AEKSGISKMSDESFAE 105
HP+L GI V+ L + + +G + S GA + ++ E F
Sbjct: 201 HPDLVGIQARVLQELGAERALVVWGRDGMDELSLGAGTLVGELRDGQVHEYEVHPEDFGI 260
Query: 106 LMTKLNSFKL-----FKAEKLQIVNQLPANMVHLYSI 137
M+ + K+ +A LQ+++ +P + + ++
Sbjct: 261 AMSASRNLKVADAAESRAMLLQVLDNVPGPALDIVAL 297
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.308 0.118 0.282
Lambda K H
0.267 0.0351 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,663,739
Number of Sequences: 36641
Number of extensions: 150183
Number of successful extensions: 382
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 362
Number of HSP's gapped (non-prelim): 41
length of query: 161
length of database: 10,336,785
effective HSP length: 89
effective length of query: 72
effective length of database: 7,075,736
effective search space: 509452992
effective search space used: 509452992
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 54 (25.6 bits)