BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P47006 (233 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N61-N nrPDB 461 e-130 3NFF-B nrPDB 104 3e-23 3ZIF-M nrPDB 30 1.0 >5N61-N nrPDB Length = 233 Score = 461 bits (1185), Expect = e-130, Method: Compositional matrix adjust. Identities = 233/233 (100%), Positives = 233/233 (100%) Query: 1 MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPS 60 MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPS Sbjct: 1 MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPS 60 Query: 61 NVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLK 120 NVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLK Sbjct: 61 NVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLK 120 Query: 121 IASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDF 180 IASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDF Sbjct: 121 IASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDF 180 Query: 181 HVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD 233 HVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD Sbjct: 181 HVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD 233 >3NFF-B nrPDB Length = 121 Score = 104 bits (260), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 57/126 (45%), Positives = 91/126 (72%), Gaps = 15/126 (11%) Query: 26 PDGFKKCKHLKNFP---LNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESS-TTMT 81 P +K+CKHLK+FP L GDNK ++WL+K P+N+DIS+LKSLP+D +++ +T+ Sbjct: 6 PSDYKQCKHLKSFPVSELKGDNK------ELWLMKVPANIDISQLKSLPLDTDATVSTVE 59 Query: 82 IDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESK-ESLKIASTAKDNAPLQFDKVFSVS 140 + ++ ++ +T + DN +N++LL+PSE K E+LK+A T+KDN + FD+VF++S Sbjct: 60 LGSKNFNVLQNTSTQEG--SDN-TNLSLLIPSEKKKETLKVA-TSKDNKSVYFDRVFTIS 115 Query: 141 ETAKIP 146 ETA+IP Sbjct: 116 ETARIP 121 >3ZIF-M nrPDB Length = 482 Score = 29.6 bits (65), Expect = 1.0, Method: Compositional matrix adjust. Identities = 26/98 (26%), Positives = 45/98 (45%), Gaps = 14/98 (14%) Query: 147 AIDYSKVRV-----PRKDVPKVEG-------LKLEHFATGYDAEDFHVAEEVKENKKEPK 194 AID++ R+ RK P EG LK + D E+F+ ++ V+ +++PK Sbjct: 236 AIDFTHSRLNNLLGIRKRFPYQEGFVISYEDLKGGNIPALMDVEEFNKSKTVRALREDPK 295 Query: 195 KRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHR 232 RS+H E+ E ++ + R + D +K R Sbjct: 296 GRSYHVGEDP--EARENETAYRSWYLAYNYGDPEKGVR 331 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N61-N nrPDB 321 2e-88 3NFF-B nrPDB 151 3e-37 Sequences not found previously or not previously below threshold: 2VNU-D nrPDB 33 0.13 3IZY-P nrPDB 32 0.23 5ZUT-A nrPDB 32 0.25 6DV6-A nrPDB 32 0.26 3K4X-A nrPDB 31 0.36 3L0X-A nrPDB 31 0.39 4L60-A nrPDB 31 0.53 6G5I-z nrPDB 30 0.83 3SE6-A nrPDB 30 0.92 5DZS-A nrPDB 29 1.6 5M2S-A nrPDB 29 2.2 3R2W-A nrPDB 28 3.3 CONVERGED! >5N61-N nrPDB Length = 233 Score = 321 bits (822), Expect = 2e-88, Method: Composition-based stats. Identities = 233/233 (100%), Positives = 233/233 (100%) Query: 1 MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPS 60 MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPS Sbjct: 1 MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPS 60 Query: 61 NVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLK 120 NVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLK Sbjct: 61 NVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLK 120 Query: 121 IASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDF 180 IASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDF Sbjct: 121 IASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDVPKVEGLKLEHFATGYDAEDF 180 Query: 181 HVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD 233 HVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD Sbjct: 181 HVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKDKKKKHRD 233 >3NFF-B nrPDB Length = 121 Score = 151 bits (380), Expect = 3e-37, Method: Composition-based stats. Identities = 57/129 (44%), Positives = 92/129 (71%), Gaps = 15/129 (11%) Query: 23 FSIPDGFKKCKHLKNFP---LNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESS-T 78 + P +K+CKHLK+FP L GDNK ++WL+K P+N+DIS+LKSLP+D +++ + Sbjct: 3 YQPPSDYKQCKHLKSFPVSELKGDNK------ELWLMKVPANIDISQLKSLPLDTDATVS 56 Query: 79 TMTIDKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESK-ESLKIASTAKDNAPLQFDKVF 137 T+ + ++ ++ +T + DN +N++LL+PSE K E+LK+A T+KDN + FD+VF Sbjct: 57 TVELGSKNFNVLQNTSTQEG--SDN-TNLSLLIPSEKKKETLKVA-TSKDNKSVYFDRVF 112 Query: 138 SVSETAKIP 146 ++SETA+IP Sbjct: 113 TISETARIP 121 >2VNU-D nrPDB Length = 760 Score = 32.8 bits (73), Expect = 0.13, Method: Composition-based stats. Identities = 14/63 (22%), Positives = 33/63 (52%), Gaps = 3/63 (4%) Query: 73 DFESSTTMTIDKHDYKIMDDTDIESSLTQDN---LSNMTLLVPSESKESLKIASTAKDNA 129 ++++ +++ +D + + D+ DIE+ DN SN T++ + + K A A+ + Sbjct: 84 EWKAPSSIVLDSEHFDVNDNPDIEAGDDDDNNESSSNTTVISDKQRRLLAKDAMIAQRSK 143 Query: 130 PLQ 132 +Q Sbjct: 144 KIQ 146 >3IZY-P nrPDB Length = 537 Score = 32.1 bits (71), Expect = 0.23, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 5/64 (7%) Query: 175 YDAEDFHVAEEVK--ENKKEPKKRSHHDDEEESSE---KKKKKKEKREKREKKDKKDKKK 229 Y+ E E++K E K++ + +H D E+ K++ + REKR+++ K K+K Sbjct: 273 YEQEQEKNKEDLKLIEEKRKEHQEAHRKDREKYGTVHWKERSYIKYREKRQQQPLKPKEK 332 Query: 230 KHRD 233 RD Sbjct: 333 LERD 336 Score = 31.7 bits (70), Expect = 0.31, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 31/53 (58%), Gaps = 6/53 (11%) Query: 183 AEEVKENKKEPKKRS------HHDDEEESSEKKKKKKEKREKREKKDKKDKKK 229 +E+ E + EP+ R + ++E++ E K +EKR++ ++ +KD++K Sbjct: 252 GDEILEVESEPRAREVVDWRKYEQEQEKNKEDLKLIEEKRKEHQEAHRKDREK 304 >5ZUT-A nrPDB Length = 289 Score = 31.7 bits (70), Expect = 0.25, Method: Composition-based stats. Identities = 28/139 (20%), Positives = 51/139 (36%), Gaps = 16/139 (11%) Query: 25 IPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTID- 83 I DGFK C L NF D ++ + + + D + M + Sbjct: 15 IIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTS 74 Query: 84 -KHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKE-----SLKIASTAKDN---APLQFD 134 + ++TD + + + ++ LL K+ SLK+ D LQ+D Sbjct: 75 LSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYD 134 Query: 135 KVFSVSETAKIPAIDYSKV 153 S +P+ ++SK+ Sbjct: 135 STLS------LPSSEFSKI 147 >6DV6-A nrPDB Length = 562 Score = 31.7 bits (70), Expect = 0.26, Method: Composition-based stats. Identities = 16/77 (20%), Positives = 34/77 (44%), Gaps = 5/77 (6%) Query: 36 KNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDI 95 KN+PL GDN+K ++ P VD+ + +D + + + + + +M + Sbjct: 134 KNYPLRGDNRKGT----FYVSGPPVYVDMVVNAATMMD-KQNDGIELGRQKIGVMRLNNT 188 Query: 96 ESSLTQDNLSNMTLLVP 112 NL + +++P Sbjct: 189 FVGDRTYNLRDQKMVIP 205 >3K4X-A nrPDB Length = 798 Score = 31.3 bits (69), Expect = 0.36, Method: Composition-based stats. Identities = 28/139 (20%), Positives = 51/139 (36%), Gaps = 16/139 (11%) Query: 25 IPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTID- 83 I DGFK C L NF D ++ + + + D + M + Sbjct: 21 IIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTS 80 Query: 84 -KHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKE-----SLKIASTAKDN---APLQFD 134 + ++TD + + + ++ LL K+ SLK+ D LQ+D Sbjct: 81 LSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYD 140 Query: 135 KVFSVSETAKIPAIDYSKV 153 S +P+ ++SK+ Sbjct: 141 STLS------LPSSEFSKI 153 Score = 31.3 bits (69), Expect = 0.36, Method: Composition-based stats. Identities = 28/139 (20%), Positives = 51/139 (36%), Gaps = 16/139 (11%) Query: 25 IPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTID- 83 I DGFK C L NF D ++ + + + D + M + Sbjct: 288 IIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTS 347 Query: 84 -KHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKE-----SLKIASTAKDN---APLQFD 134 + ++TD + + + ++ LL K+ SLK+ D LQ+D Sbjct: 348 LSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYD 407 Query: 135 KVFSVSETAKIPAIDYSKV 153 S +P+ ++SK+ Sbjct: 408 STLS------LPSSEFSKI 420 Score = 31.3 bits (69), Expect = 0.36, Method: Composition-based stats. Identities = 28/139 (20%), Positives = 51/139 (36%), Gaps = 16/139 (11%) Query: 25 IPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTID- 83 I DGFK C L NF D ++ + + + D + M + Sbjct: 555 IIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTS 614 Query: 84 -KHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKE-----SLKIASTAKDN---APLQFD 134 + ++TD + + + ++ LL K+ SLK+ D LQ+D Sbjct: 615 LSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYD 674 Query: 135 KVFSVSETAKIPAIDYSKV 153 S +P+ ++SK+ Sbjct: 675 STLS------LPSSEFSKI 687 >3L0X-A nrPDB Length = 169 Score = 31.3 bits (69), Expect = 0.39, Method: Composition-based stats. Identities = 28/139 (20%), Positives = 51/139 (36%), Gaps = 16/139 (11%) Query: 25 IPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTID- 83 I DGFK C L NF D ++ + + + D + M + Sbjct: 21 IIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTS 80 Query: 84 -KHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKE-----SLKIASTAKDN---APLQFD 134 + ++TD + + + ++ LL K+ SLK+ D LQ+D Sbjct: 81 LSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYD 140 Query: 135 KVFSVSETAKIPAIDYSKV 153 S +P+ ++SK+ Sbjct: 141 STLS------LPSSEFSKI 153 >4L60-A nrPDB Length = 256 Score = 30.9 bits (68), Expect = 0.53, Method: Composition-based stats. Identities = 30/140 (21%), Positives = 53/140 (37%), Gaps = 18/140 (12%) Query: 25 IPDGFKKCKHLKNFPLNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTIDK 84 I DGFK C L NF D ++ + + + D + M + Sbjct: 15 IIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTS 74 Query: 85 HDYKIM---DDTDIESSLTQDNLSNMTLLVPSESKE-----SLKIASTAKDN---APLQF 133 KI+ ++TD + + + ++ LL K+ SLK+ D LQ+ Sbjct: 75 LS-KILRRGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQY 133 Query: 134 DKVFSVSETAKIPAIDYSKV 153 D S +P+ ++SK+ Sbjct: 134 DSTLS------LPSSEFSKI 147 >6G5I-z nrPDB Length = 568 Score = 30.1 bits (66), Expect = 0.83, Method: Composition-based stats. Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 6/64 (9%) Query: 173 TGYDAEDFHVAE------EVKENKKEPKKRSHHDDEEESSEKKKKKKEKREKREKKDKKD 226 T D+ED +E E + + PKK + D ++ KK K+ +REKR+ K K Sbjct: 494 TCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKH 553 Query: 227 KKKK 230 KK+ Sbjct: 554 VKKR 557 >3SE6-A nrPDB Length = 967 Score = 30.1 bits (66), Expect = 0.92, Method: Composition-based stats. Identities = 31/152 (20%), Positives = 64/152 (42%), Gaps = 30/152 (19%) Query: 19 ISNEFSIPDGFKKCKHLKNFPLNGDNKKKAKQQQVWL--IKFPSNVDISKLKSL-PVDFE 75 I ++FS+P + + FP+ N ++ Q++ L + P + D+ +L +DF Sbjct: 34 ICSQFSVPSSYHFTEDPGAFPV-ATNGERFPWQELRLPSVVIPLHYDLFVHPNLTSLDFV 92 Query: 76 SSTTMTI---DKHDYKIMDDTDIESSLTQDNLSNMTLLVPSESKESLKIASTAKDNAPLQ 132 +S + + + + I+ D+E ++N TL +S+E + K+ L Sbjct: 93 ASEKIEVLVSNATQFIILHSKDLE-------ITNATL----QSEEDSRYMKPGKELKVLS 141 Query: 133 FDKVFSVSETAKIPAIDYSKVRVPRKDVPKVE 164 + PA + + VP K P ++ Sbjct: 142 Y------------PAHEQIALLVPEKLTPHLK 161 >5DZS-A nrPDB Length = 276 Score = 29.4 bits (64), Expect = 1.6, Method: Composition-based stats. Identities = 12/35 (34%), Positives = 20/35 (57%) Query: 2 SKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLK 36 S + K YVS DD+ + N +I +++ KH+K Sbjct: 140 SGIEKLYVSTRKKDDKKLLNSKAILIDYEELKHIK 174 >5M2S-A nrPDB Length = 149 Score = 28.6 bits (62), Expect = 2.2, Method: Composition-based stats. Identities = 17/78 (21%), Positives = 35/78 (44%) Query: 40 LNGDNKKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSL 99 ++ NK + V+ I P +D +SL + T + I ++K +DD Sbjct: 30 VDSKNKAISAMDVVFAIDSPLTIDEIDKESLAFKTTAMTNIAILGANFKSLDDKGEPLVP 89 Query: 100 TQDNLSNMTLLVPSESKE 117 T+D + + + VP+ + + Sbjct: 90 TKDPVFTLYVTVPATTPD 107 >3R2W-A nrPDB Length = 528 Score = 28.2 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 21/89 (23%), Positives = 37/89 (41%), Gaps = 14/89 (15%) Query: 83 DKHDYKIMDDTDIESSLTQDNLSNMTLLVPSE-----SKESLKIASTAKDNAPLQ----- 132 H + + D +++QD S ++ E KE KI +TA + Sbjct: 18 GSHMSRFVQDLS--KAMSQDGASQFQEVIRQELELSVKKELEKILTTASSHEFEHTKKDL 75 Query: 133 --FDKVFSVSETAKIPAIDYSKVRVPRKD 159 F K+F K P++D+ K++ P +D Sbjct: 76 DGFRKLFHRFLQEKGPSVDWGKIQRPPED 104 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.303 0.125 0.335 Lambda K H 0.267 0.0393 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,989,404 Number of Sequences: 36641 Number of extensions: 355590 Number of successful extensions: 2766 Number of sequences better than 10.0: 53 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 67 Number of HSP's that attempted gapping in prelim test: 1328 Number of HSP's gapped (non-prelim): 853 length of query: 233 length of database: 10,336,785 effective HSP length: 94 effective length of query: 139 effective length of database: 6,892,531 effective search space: 958061809 effective search space used: 958061809 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.8 bits) S2: 57 (26.6 bits)