BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P46984 (123 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4WXA-D nrPDB 250 1e-67 5V4P-A nrPDB 29 0.56 6B9O-A nrPDB 27 2.0 5DIP-A nrPDB 27 2.6 4RNX-A nrPDB 27 3.0 4RNU-A nrPDB 27 3.1 4GBU-A nrPDB 26 3.2 5OEV-A nrPDB 26 3.8 2PA6-A nrPDB 26 4.7 >4WXA-D nrPDB Length = 123 Score = 250 bits (638), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 123/123 (100%), Positives = 123/123 (100%) Query: 1 MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKD 60 MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKD Sbjct: 1 MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKD 60 Query: 61 GSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDE 120 GSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDE Sbjct: 61 GSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDE 120 Query: 121 DAV 123 DAV Sbjct: 121 DAV 123 >5V4P-A nrPDB Length = 400 Score = 28.9 bits (63), Expect = 0.56, Method: Compositional matrix adjust. Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 12/63 (19%) Query: 56 ERTKDGSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQ 111 E+ KD + L + LT +DDI +++ + AKN AGAD I +NQ Sbjct: 150 EKAKDANNLEH------SLT--KDDIKQYIKDYIHAAKNSIAAGADGVEIHSANGYLLNQ 201 Query: 112 LLD 114 LD Sbjct: 202 FLD 204 >6B9O-A nrPDB Length = 981 Score = 26.9 bits (58), Expect = 2.0, Method: Compositional matrix adjust. Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 6/49 (12%) Query: 7 QYSAPDG----VEKSFAPIRDDPRYMTT--EGRTTGPSDHVLNAGQIDR 49 Y P+G V +F P++DDPR T E R D L +I R Sbjct: 209 HYGPPNGFNFEVRNNFVPLQDDPRLFDTNVEERVQNFIDAALTQAKITR 257 >5DIP-A nrPDB Length = 121 Score = 26.6 bits (57), Expect = 2.6, Method: Compositional matrix adjust. Identities = 15/44 (34%), Positives = 24/44 (54%) Query: 70 LRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLL 113 + TQL + ++ E +TG LA+ K GA +K+ +E I L Sbjct: 20 ITTQLAKFRKEMPELMTGFSSLAQAATKDGALDKKTKELIAMAL 63 >4RNX-A nrPDB Length = 400 Score = 26.6 bits (57), Expect = 3.0, Method: Compositional matrix adjust. Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 78 QDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQLLD 114 +D+I +++ ++ AKN AGAD I +NQ LD Sbjct: 12 KDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLD 52 >4RNU-A nrPDB Length = 400 Score = 26.6 bits (57), Expect = 3.1, Method: Compositional matrix adjust. Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 78 QDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQLLD 114 +D+I +++ ++ AKN AGAD I +NQ LD Sbjct: 262 KDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLD 302 >4GBU-A nrPDB Length = 400 Score = 26.2 bits (56), Expect = 3.2, Method: Compositional matrix adjust. Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 78 QDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQLLD 114 +D+I +++ ++ AKN AGAD I +NQ LD Sbjct: 164 KDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLD 204 >5OEV-A nrPDB Length = 510 Score = 26.2 bits (56), Expect = 3.8, Method: Compositional matrix adjust. Identities = 13/47 (27%), Positives = 27/47 (57%) Query: 69 QLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDG 115 Q + +L +Q + E G+M++ KAG ++ ++ E+ + LL+G Sbjct: 244 QYKFELRNIQCQLEELSNGQMKVEYVSLKAGYEQLKLGEDYSLLLNG 290 >2PA6-A nrPDB Length = 427 Score = 25.8 bits (55), Expect = 4.7, Method: Compositional matrix adjust. Identities = 15/40 (37%), Positives = 19/40 (47%) Query: 30 TEGRTTGPSDHVLNAGQIDRDKPSEPERTKDGSQLTYLGQ 69 TE T LN+GQI P+ ERT +QL + Q Sbjct: 372 TEDTTIADLSVALNSGQIKTGAPARGERTAKYNQLIRIEQ 411 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4WXA-D nrPDB 240 1e-64 Sequences not found previously or not previously below threshold: 5V4P-A nrPDB 31 0.14 6B9O-A nrPDB 30 0.18 5DIP-A nrPDB 29 0.47 3TFC-A nrPDB 29 0.79 2PA6-A nrPDB 28 1.1 5OEV-A nrPDB 28 1.1 4RNX-A nrPDB 27 1.4 4RNU-A nrPDB 27 1.4 4GBU-A nrPDB 27 1.4 5GM5-A nrPDB 26 5.2 4XMN-B nrPDB 25 5.8 CONVERGED! >4WXA-D nrPDB Length = 123 Score = 240 bits (612), Expect = 1e-64, Method: Composition-based stats. Identities = 123/123 (100%), Positives = 123/123 (100%) Query: 1 MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKD 60 MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKD Sbjct: 1 MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKD 60 Query: 61 GSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDE 120 GSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDE Sbjct: 61 GSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDE 120 Query: 121 DAV 123 DAV Sbjct: 121 DAV 123 >5V4P-A nrPDB Length = 400 Score = 30.8 bits (68), Expect = 0.14, Method: Composition-based stats. Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 12/63 (19%) Query: 56 ERTKDGSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQ 111 E+ KD + L + LT +DDI +++ + AKN AGAD I +NQ Sbjct: 150 EKAKDANNLEH------SLT--KDDIKQYIKDYIHAAKNSIAAGADGVEIHSANGYLLNQ 201 Query: 112 LLD 114 LD Sbjct: 202 FLD 204 >6B9O-A nrPDB Length = 981 Score = 30.4 bits (67), Expect = 0.18, Method: Composition-based stats. Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 6/50 (12%) Query: 6 AQYSAPDG----VEKSFAPIRDDPRYMTT--EGRTTGPSDHVLNAGQIDR 49 Y P+G V +F P++DDPR T E R D L +I R Sbjct: 208 GHYGPPNGFNFEVRNNFVPLQDDPRLFDTNVEERVQNFIDAALTQAKITR 257 >5DIP-A nrPDB Length = 121 Score = 29.3 bits (64), Expect = 0.47, Method: Composition-based stats. Identities = 15/44 (34%), Positives = 24/44 (54%) Query: 70 LRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLL 113 + TQL + ++ E +TG LA+ K GA +K+ +E I L Sbjct: 20 ITTQLAKFRKEMPELMTGFSSLAQAATKDGALDKKTKELIAMAL 63 >3TFC-A nrPDB Length = 385 Score = 28.5 bits (62), Expect = 0.79, Method: Composition-based stats. Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 7/61 (11%) Query: 65 TYLGQLRTQLTGLQDDINEFLTGR----MELAKNKKKAGA---DEKRIQEEINQLLDGGD 117 T L +LRTQ+ L D+ E ++ R E+ K K G+ D R +E +N +L + Sbjct: 28 TNLEELRTQVDQLNIDLLELISKRANLVQEIGKIKGTQGSLRFDPLREREMLNTILAANE 87 Query: 118 G 118 G Sbjct: 88 G 88 >2PA6-A nrPDB Length = 427 Score = 28.1 bits (61), Expect = 1.1, Method: Composition-based stats. Identities = 16/44 (36%), Positives = 20/44 (45%) Query: 26 RYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKDGSQLTYLGQ 69 R TE T LN+GQI P+ ERT +QL + Q Sbjct: 368 RSGETEDTTIADLSVALNSGQIKTGAPARGERTAKYNQLIRIEQ 411 >5OEV-A nrPDB Length = 510 Score = 27.7 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 13/47 (27%), Positives = 27/47 (57%) Query: 69 QLRTQLTGLQDDINEFLTGRMELAKNKKKAGADEKRIQEEINQLLDG 115 Q + +L +Q + E G+M++ KAG ++ ++ E+ + LL+G Sbjct: 244 QYKFELRNIQCQLEELSNGQMKVEYVSLKAGYEQLKLGEDYSLLLNG 290 >4RNX-A nrPDB Length = 400 Score = 27.4 bits (59), Expect = 1.4, Method: Composition-based stats. Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 78 QDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQLLD 114 +D+I +++ ++ AKN AGAD I +NQ LD Sbjct: 12 KDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLD 52 >4RNU-A nrPDB Length = 400 Score = 27.4 bits (59), Expect = 1.4, Method: Composition-based stats. Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 78 QDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQLLD 114 +D+I +++ ++ AKN AGAD I +NQ LD Sbjct: 262 KDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLD 302 >4GBU-A nrPDB Length = 400 Score = 27.4 bits (59), Expect = 1.4, Method: Composition-based stats. Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Query: 78 QDDINEFLTGRMELAKNKKKAGADEKRIQEE----INQLLD 114 +D+I +++ ++ AKN AGAD I +NQ LD Sbjct: 164 KDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLD 204 >5GM5-A nrPDB Length = 220 Score = 25.8 bits (55), Expect = 5.2, Method: Composition-based stats. Identities = 11/26 (42%), Positives = 15/26 (57%) Query: 60 DGSQLTYLGQLRTQLTGLQDDINEFL 85 +GSQ TY T +T Q D+N+F Sbjct: 152 NGSQKTYSFVAPTPITSFQGDVNDFF 177 >4XMN-B nrPDB Length = 652 Score = 25.4 bits (54), Expect = 5.8, Method: Composition-based stats. Identities = 21/82 (25%), Positives = 38/82 (46%), Gaps = 6/82 (7%) Query: 38 SDHVLNAGQIDRDKPSEPERTKDGSQLTYLGQLRTQLTGLQDDINEF-----LTGRMELA 92 +DH+LN +I + KD + YL +++ L G D+ E L R+ L+ Sbjct: 464 NDHILNRLKIPSQLIFNAQALKDRYEGNYLSEVQNLLLGSSYDLAEMAIVTSLGPRLLLS 523 Query: 93 KNKKKAGADEKRIQEEINQLLD 114 N + + K ++E +N+ D Sbjct: 524 NNPVQ-NNELKTLREILNEFPD 544 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.132 0.369 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,270,798 Number of Sequences: 36641 Number of extensions: 203659 Number of successful extensions: 369 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 356 Number of HSP's gapped (non-prelim): 30 length of query: 123 length of database: 10,336,785 effective HSP length: 85 effective length of query: 38 effective length of database: 7,222,300 effective search space: 274447400 effective search space used: 274447400 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 53 (25.0 bits)