BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P46669 (326 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N5Z-G nrPDB 665 0.0 2RF4-A nrPDB 431 e-121 4PFT-A nrPDB 30 1.6 4K3B-A nrPDB 30 1.9 4J9X-A nrPDB 29 3.3 6F4A-B nrPDB 28 3.9 2BNU-B nrPDB 28 6.7 >5N5Z-G nrPDB Length = 326 Score = 665 bits (1716), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/326 (100%), Positives = 326/326 (100%) Query: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG Sbjct: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 Query: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV Sbjct: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 Query: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI 180 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI Sbjct: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI 180 Query: 181 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 240 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG Sbjct: 181 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 240 Query: 241 RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV 300 RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV Sbjct: 241 RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV 300 Query: 301 KEDNGSEIVYEENTSESNDGESSDSD 326 KEDNGSEIVYEENTSESNDGESSDSD Sbjct: 301 KEDNGSEIVYEENTSESNDGESSDSD 326 >2RF4-A nrPDB Length = 214 Score = 431 bits (1109), Expect = e-121, Method: Compositional matrix adjust. Identities = 214/251 (85%), Positives = 214/251 (85%), Gaps = 37/251 (14%) Query: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG Sbjct: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 Query: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV Sbjct: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 Query: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI 180 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHND Sbjct: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHND-------- 172 Query: 181 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 240 GNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG Sbjct: 173 -----------------------------GNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 203 Query: 241 RVVSVDGTLIS 251 RVVSVDGTLIS Sbjct: 204 RVVSVDGTLIS 214 >4PFT-A nrPDB Length = 544 Score = 30.0 bits (66), Expect = 1.6, Method: Compositional matrix adjust. Identities = 20/64 (31%), Positives = 34/64 (53%), Gaps = 5/64 (7%) Query: 57 PLQGVMKQHLNPLV----MKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTP 112 PL G MK + +V KY+ ++ +L G K ++ D +ED + K K+T + P Sbjct: 307 PLPGYMKYYPKEVVDKYGFKYDPEMAKKILDELGFKDVNKDGF-REDPNGKPFKLTIECP 365 Query: 113 FGFT 116 +G+T Sbjct: 366 YGWT 369 >4K3B-A nrPDB Length = 792 Score = 29.6 bits (65), Expect = 1.9, Method: Compositional matrix adjust. Identities = 15/40 (37%), Positives = 21/40 (52%) Query: 83 GYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHVNL 122 GY +ILD D + ED + + IKIT F W V++ Sbjct: 233 GYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSI 272 >4J9X-A nrPDB Length = 332 Score = 28.9 bits (63), Expect = 3.3, Method: Compositional matrix adjust. Identities = 11/31 (35%), Positives = 17/31 (54%) Query: 120 VNLYVWQPQVGDVLEGYIFIQSASHIGLLIH 150 V + QPQ GD G IF ++ ++G+ H Sbjct: 82 VGALLCQPQAGDACAGVIFFNNSGYLGMCGH 112 >6F4A-B nrPDB Length = 644 Score = 28.5 bits (62), Expect = 3.9, Method: Compositional matrix adjust. Identities = 27/94 (28%), Positives = 39/94 (41%), Gaps = 10/94 (10%) Query: 199 GGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTGRVVSVDGTL------ISD 252 G +NS F RSL ++ G LR R V+ + +V L I D Sbjct: 166 GPTNSGKTF----RSLQKLKAADRGYYAGPLRLLAREVYEKFKHENVRCNLLTGEEVIKD 221 Query: 253 ADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSI 286 DE GN N + E +P+ N +V DE+ + Sbjct: 222 LDEMGNEANLTSGTIEMIPLNQNFDVVVLDEIQM 255 >2BNU-B nrPDB Length = 241 Score = 27.7 bits (60), Expect = 6.7, Method: Compositional matrix adjust. Identities = 13/26 (50%), Positives = 15/26 (57%) Query: 247 GTLISDADEEGNGYNSSRSQAESLPI 272 G I+D E NGYN SRS E P+ Sbjct: 49 GAGITDQGEVPNGYNVSRSTTEDFPL 74 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N5Z-G nrPDB 634 0.0 2RF4-A nrPDB 452 e-127 Sequences not found previously or not previously below threshold: 4PFT-A nrPDB 32 0.36 4K3B-A nrPDB 31 0.84 4J9X-A nrPDB 29 3.5 5DN6-A nrPDB 28 4.8 3T6C-A nrPDB 28 5.0 4EQB-A nrPDB 28 5.5 CONVERGED! >5N5Z-G nrPDB Length = 326 Score = 634 bits (1634), Expect = 0.0, Method: Composition-based stats. Identities = 326/326 (100%), Positives = 326/326 (100%) Query: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG Sbjct: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 Query: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV Sbjct: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 Query: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI 180 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI Sbjct: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI 180 Query: 181 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 240 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG Sbjct: 181 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 240 Query: 241 RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV 300 RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV Sbjct: 241 RVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNKKIVFDDEVSIENKESHKELDLPEV 300 Query: 301 KEDNGSEIVYEENTSESNDGESSDSD 326 KEDNGSEIVYEENTSESNDGESSDSD Sbjct: 301 KEDNGSEIVYEENTSESNDGESSDSD 326 >2RF4-A nrPDB Length = 214 Score = 452 bits (1162), Expect = e-127, Method: Composition-based stats. Identities = 214/251 (85%), Positives = 214/251 (85%), Gaps = 37/251 (14%) Query: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG Sbjct: 1 MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIALYVSLAPMYLENPLQG 60 Query: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV Sbjct: 61 VMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHV 120 Query: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHNDVEEDADVI 180 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHND Sbjct: 121 NLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKNNIPVDWTFVHND-------- 172 Query: 181 NTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 240 GNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG Sbjct: 173 -----------------------------GNRSLGHWVDSNGEPIDGKLRFTVRNVHTTG 203 Query: 241 RVVSVDGTLIS 251 RVVSVDGTLIS Sbjct: 204 RVVSVDGTLIS 214 >4PFT-A nrPDB Length = 544 Score = 32.0 bits (71), Expect = 0.36, Method: Composition-based stats. Identities = 20/64 (31%), Positives = 34/64 (53%), Gaps = 5/64 (7%) Query: 57 PLQGVMKQHLNPLV----MKYNNKVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTP 112 PL G MK + +V KY+ ++ +L G K ++ D +ED + K K+T + P Sbjct: 307 PLPGYMKYYPKEVVDKYGFKYDPEMAKKILDELGFKDVNKDGF-REDPNGKPFKLTIECP 365 Query: 113 FGFT 116 +G+T Sbjct: 366 YGWT 369 >4K3B-A nrPDB Length = 792 Score = 30.9 bits (68), Expect = 0.84, Method: Composition-based stats. Identities = 15/40 (37%), Positives = 21/40 (52%) Query: 83 GYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHVNL 122 GY +ILD D + ED + + IKIT F W V++ Sbjct: 233 GYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSI 272 >4J9X-A nrPDB Length = 332 Score = 28.9 bits (63), Expect = 3.5, Method: Composition-based stats. Identities = 10/25 (40%), Positives = 15/25 (60%) Query: 126 QPQVGDVLEGYIFIQSASHIGLLIH 150 QPQ GD G IF ++ ++G+ H Sbjct: 88 QPQAGDACAGVIFFNNSGYLGMCGH 112 >5DN6-A nrPDB Length = 511 Score = 28.2 bits (61), Expect = 4.8, Method: Composition-based stats. Identities = 13/42 (30%), Positives = 22/42 (52%) Query: 187 GNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWVDSNGEPIDGK 228 G++ + D+ + ++ + G LG VD+ G PIDGK Sbjct: 77 GDDRDIKEGDTVKRTGAIVEVPAGKELLGRVVDALGNPIDGK 118 >3T6C-A nrPDB Length = 440 Score = 28.2 bits (61), Expect = 5.0, Method: Composition-based stats. Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 1/46 (2%) Query: 93 DPLSKEDTSE-KLIKITPDTPFGFTWCHVNLYVWQPQVGDVLEGYI 137 DP++ E+T K+++ TP VN+ W+P + + L YI Sbjct: 273 DPVAPENTEWLKMLRQQSSTPIAMGELFVNVNEWKPLIDNKLIDYI 318 >4EQB-A nrPDB Length = 330 Score = 28.2 bits (61), Expect = 5.5, Method: Composition-based stats. Identities = 23/85 (27%), Positives = 39/85 (45%), Gaps = 15/85 (17%) Query: 76 KVGGVVLGYEGLKILDADPLSKEDTSEKLIKITPDTPFGFTWCHVNLYVWQPQVGDVLEG 135 +V G+ L G + DP E+T +KL K+TP+ + V D ++G Sbjct: 151 EVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNI--------------KAIVADEMKG 196 Query: 136 YIFIQSASHIGLLIHDAFNASIKKN 160 Y+ IQ+ IG+ + ++KN Sbjct: 197 YM-IQNNVAIGVTFSGEASQMLEKN 220 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.129 0.380 Lambda K H 0.267 0.0388 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,015,912 Number of Sequences: 36641 Number of extensions: 633330 Number of successful extensions: 1281 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 21 Number of HSP's successfully gapped in prelim test: 24 Number of HSP's that attempted gapping in prelim test: 1197 Number of HSP's gapped (non-prelim): 73 length of query: 326 length of database: 10,336,785 effective HSP length: 97 effective length of query: 229 effective length of database: 6,782,608 effective search space: 1553217232 effective search space used: 1553217232 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 59 (27.4 bits)