BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P45850 (201 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2ET1-A nrPDB 407 e-114 2VQA-A nrPDB 37 0.008 3HT1-A nrPDB 31 0.29 4V8P-BA nrPDB 31 0.37 2PHL-A nrPDB 30 0.87 4QM9-A nrPDB 30 0.95 6HD7-E nrPDB 28 2.2 3LWA-A nrPDB 28 2.3 5IT7-AA nrPDB 28 3.3 3WW2-A nrPDB 28 3.7 2IZ1-A nrPDB 27 4.5 1UN9-A nrPDB 27 6.4 >2ET1-A nrPDB Length = 201 Score = 407 bits (1047), Expect = e-114, Method: Compositional matrix adjust. Identities = 200/201 (99%), Positives = 201/201 (100%) Query: 1 SDPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELD 60 +DPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELD Sbjct: 1 TDPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELD 60 Query: 61 VAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYS 120 VAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYS Sbjct: 61 VAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYS 120 Query: 121 RVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLT 180 RVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLT Sbjct: 121 RVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLT 180 Query: 181 KALRVEAGVVELLKSKFAGGS 201 KALRVEAGVVELLKSKFAGGS Sbjct: 181 KALRVEAGVVELLKSKFAGGS 201 >2VQA-A nrPDB Length = 361 Score = 36.6 bits (83), Expect = 0.008, Method: Compositional matrix adjust. Identities = 36/137 (26%), Positives = 62/137 (45%), Gaps = 13/137 (9%) Query: 63 EWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRV 122 E+PG+ + ++ + PG H HP A E V+ GE+ + + S G SR Sbjct: 227 EFPGSFNMTGAL--IHLEPGAMRQLHWHPNADEWQYVLDGEMDLTVFAS--EGKASVSR- 281 Query: 123 VRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFV-------PLTLFGSDPPIP 175 ++ G+ +P+G H N + +VV FN + + + P ++ G+ I Sbjct: 282 LQQGDVGYVPKGYGHAIRNSSQKPLDIVVVFNDGDYQSIDLSTWLASNPSSVLGNTFQI- 340 Query: 176 TPVLTKALRVEAGVVEL 192 +P LTK L V+ + L Sbjct: 341 SPELTKKLPVQDTIFSL 357 Score = 28.5 bits (62), Expect = 2.0, Method: Compositional matrix adjust. Identities = 21/84 (25%), Positives = 32/84 (38%), Gaps = 3/84 (3%) Query: 71 GVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRVVRAGETFV 130 G++ + PG H H A E VM+G + + K+ V G + Sbjct: 51 GMAGVYMSLEPGAIRELHWHANAAEWAYVMEGRTRITL---TSPEGKVEIADVDKGGLWY 107 Query: 131 IPRGLMHFQFNVGKTEAYMVVSFN 154 PRG H +G A ++ FN Sbjct: 108 FPRGWGHSIEGIGPDTAKFLLVFN 131 >3HT1-A nrPDB Length = 145 Score = 31.2 bits (69), Expect = 0.29, Method: Compositional matrix adjust. Identities = 27/88 (30%), Positives = 40/88 (45%), Gaps = 12/88 (13%) Query: 74 MNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSL--DSGNKLYSRVVRAGETFVI 131 + + +P G+ PPH H EI +L G +G + D G + V GE I Sbjct: 41 LTEFEVSPNGSTPPHFHEWEHEIY------VLEGSMGLVLPDQGR---TEEVGPGEAIFI 91 Query: 132 PRGLMH-FQFNVGKTEAYMVVSFNSQNP 158 PRG H F G+T ++VV+ + P Sbjct: 92 PRGEPHGFVTGPGQTCRFLVVAPCERPP 119 >4V8P-BA nrPDB Length = 264 Score = 30.8 bits (68), Expect = 0.37, Method: Compositional matrix adjust. Identities = 24/74 (32%), Positives = 32/74 (43%), Gaps = 21/74 (28%) Query: 19 VSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELDVAEWPGTNTLGVSMNRVD 78 V+ G T KP+ +AG+ F ++ K+ WP GV+MN VD Sbjct: 170 VAGGGRTDKPILKAGNQFHKYARKRKS------------------WP--RVRGVAMNPVD 209 Query: 79 FAPGGTNPPHI-HP 91 GG N HI HP Sbjct: 210 HPHGGGNHQHIGHP 223 >2PHL-A nrPDB Length = 397 Score = 29.6 bits (65), Expect = 0.87, Method: Compositional matrix adjust. Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 4/69 (5%) Query: 68 NTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRVVRA-- 125 N+L V ++ ++ G PH + +A I +V +GE V ++G + L RA Sbjct: 235 NSLNVLISSIEMEEGALFVPHYYSKAIVILVVNEGEAHVELVGPKGNKETLEYESYRAEL 294 Query: 126 --GETFVIP 132 + FVIP Sbjct: 295 SKDDVFVIP 303 >4QM9-A nrPDB Length = 173 Score = 29.6 bits (65), Expect = 0.95, Method: Compositional matrix adjust. Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Query: 77 VDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLY-SRVVRAGETFVIPRGL 135 ++ P H H ++ MV++G+LL I S +L S V GE + +GL Sbjct: 76 INIPPNKETTVHDHGQSIGCAMVLEGKLLNSIYRSTGEHAELSNSYFVHEGECLISTKGL 135 Query: 136 MH 137 +H Sbjct: 136 IH 137 >6HD7-E nrPDB Length = 254 Score = 28.5 bits (62), Expect = 2.2, Method: Compositional matrix adjust. Identities = 15/32 (46%), Positives = 16/32 (50%), Gaps = 2/32 (6%) Query: 58 ELDVAEWPGTNTLGVSMNRVDFAPGGTNPPHI 89 L WP T GV+MN VD GG N HI Sbjct: 190 RLKRNSWP--KTRGVAMNPVDHPHGGGNHQHI 219 >3LWA-A nrPDB Length = 183 Score = 28.1 bits (61), Expect = 2.3, Method: Compositional matrix adjust. Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 3/49 (6%) Query: 11 VADLDGKAVSVN--GHTCKPMSEAGDDF-LFSSKLTKAGNTSTPNGSAV 56 ++D + + V +N G C P DD + +L AGN TP G+ + Sbjct: 54 LSDFENQVVILNAWGQWCAPCRSESDDLQIIHEELQAAGNGDTPGGTVL 102 >5IT7-AA nrPDB Length = 249 Score = 27.7 bits (60), Expect = 3.3, Method: Compositional matrix adjust. Identities = 14/26 (53%), Positives = 15/26 (57%), Gaps = 2/26 (7%) Query: 64 WPGTNTLGVSMNRVDFAPGGTNPPHI 89 WP T GV+MN VD GG N HI Sbjct: 195 WP--KTRGVAMNPVDHPHGGGNHQHI 218 >3WW2-A nrPDB Length = 256 Score = 27.7 bits (60), Expect = 3.7, Method: Compositional matrix adjust. Identities = 13/26 (50%), Positives = 15/26 (57%) Query: 81 PGGTNPPHIHPRATEIGMVMKGELLV 106 PG PPH H R TE V+ GE+ V Sbjct: 106 PGKFCPPHFHQRKTESYEVVLGEMEV 131 >2IZ1-A nrPDB Length = 474 Score = 27.3 bits (59), Expect = 4.5, Method: Compositional matrix adjust. Identities = 12/56 (21%), Positives = 26/56 (46%) Query: 6 LQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELDV 61 +++ +LD + + K + G+ ++ L KAGN T ++ + LD+ Sbjct: 222 FEEWNEGELDSYLIEITKEVLKRKDDEGEGYIVDKILDKAGNKGTGKWTSESALDL 277 >1UN9-A nrPDB Length = 552 Score = 26.9 bits (58), Expect = 6.4, Method: Compositional matrix adjust. Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 2/49 (4%) Query: 57 TELDVAEWPGTNT--LGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGE 103 T L A++ +NT LGV+++ P HP E+GM + GE Sbjct: 174 TVLREAQYAASNTFSLGVALSSCHLPQETDAAPRHHPGHAELGMGIHGE 222 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2ET1-A nrPDB 406 e-114 Sequences not found previously or not previously below threshold: 2VQA-A nrPDB 39 0.002 3HT1-A nrPDB 33 0.086 4V8P-BA nrPDB 32 0.21 2PHL-A nrPDB 30 0.80 4QM9-A nrPDB 30 0.85 6HD7-E nrPDB 29 1.2 5IT7-AA nrPDB 29 1.2 3LWA-A nrPDB 29 1.6 3WW2-A nrPDB 28 2.1 1UN9-A nrPDB 28 3.9 5XXB-A nrPDB 28 4.0 4JJP-A nrPDB 27 4.8 >2ET1-A nrPDB Length = 201 Score = 406 bits (1044), Expect = e-114, Method: Composition-based stats. Identities = 200/201 (99%), Positives = 201/201 (100%) Query: 1 SDPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELD 60 +DPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELD Sbjct: 1 TDPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELD 60 Query: 61 VAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYS 120 VAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYS Sbjct: 61 VAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYS 120 Query: 121 RVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLT 180 RVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLT Sbjct: 121 RVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLT 180 Query: 181 KALRVEAGVVELLKSKFAGGS 201 KALRVEAGVVELLKSKFAGGS Sbjct: 181 KALRVEAGVVELLKSKFAGGS 201 >2VQA-A nrPDB Length = 361 Score = 38.8 bits (89), Expect = 0.002, Method: Composition-based stats. Identities = 36/137 (26%), Positives = 62/137 (45%), Gaps = 13/137 (9%) Query: 63 EWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRV 122 E+PG+ + ++ + PG H HP A E V+ GE+ + + S G SR Sbjct: 227 EFPGSFNMTGAL--IHLEPGAMRQLHWHPNADEWQYVLDGEMDLTVFAS--EGKASVSR- 281 Query: 123 VRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFV-------PLTLFGSDPPIP 175 ++ G+ +P+G H N + +VV FN + + + P ++ G+ I Sbjct: 282 LQQGDVGYVPKGYGHAIRNSSQKPLDIVVVFNDGDYQSIDLSTWLASNPSSVLGNTFQI- 340 Query: 176 TPVLTKALRVEAGVVEL 192 +P LTK L V+ + L Sbjct: 341 SPELTKKLPVQDTIFSL 357 Score = 26.9 bits (58), Expect = 5.7, Method: Composition-based stats. Identities = 25/104 (24%), Positives = 40/104 (38%), Gaps = 7/104 (6%) Query: 52 NGSAVTELDVAEWPGTNTL-GVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILG 110 +G ++ +P + + GV M+ PG H H A E VM+G + + Sbjct: 34 DGGTTKQVGTYNFPVSKGMAGVYMS---LEPGAIRELHWHANAAEWAYVMEGRTRITL-- 88 Query: 111 SLDSGNKLYSRVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFN 154 K+ V G + PRG H +G A ++ FN Sbjct: 89 -TSPEGKVEIADVDKGGLWYFPRGWGHSIEGIGPDTAKFLLVFN 131 >3HT1-A nrPDB Length = 145 Score = 33.0 bits (74), Expect = 0.086, Method: Composition-based stats. Identities = 26/80 (32%), Positives = 40/80 (50%), Gaps = 8/80 (10%) Query: 74 MNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRVVRAGETFVIPR 133 + + +P G+ PPH H EI V++G + G++ D G + V GE IPR Sbjct: 41 LTEFEVSPNGSTPPHFHEWEHEI-YVLEGSM--GLVLP-DQGR---TEEVGPGEAIFIPR 93 Query: 134 GLMH-FQFNVGKTEAYMVVS 152 G H F G+T ++VV+ Sbjct: 94 GEPHGFVTGPGQTCRFLVVA 113 >4V8P-BA nrPDB Length = 264 Score = 31.9 bits (71), Expect = 0.21, Method: Composition-based stats. Identities = 24/74 (32%), Positives = 32/74 (43%), Gaps = 21/74 (28%) Query: 19 VSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELDVAEWPGTNTLGVSMNRVD 78 V+ G T KP+ +AG+ F ++ K+ WP GV+MN VD Sbjct: 170 VAGGGRTDKPILKAGNQFHKYARKRKS------------------WP--RVRGVAMNPVD 209 Query: 79 FAPGGTNPPHI-HP 91 GG N HI HP Sbjct: 210 HPHGGGNHQHIGHP 223 >2PHL-A nrPDB Length = 397 Score = 30.0 bits (66), Expect = 0.80, Method: Composition-based stats. Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 4/69 (5%) Query: 68 NTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRVVRA-- 125 N+L V ++ ++ G PH + +A I +V +GE V ++G + L RA Sbjct: 235 NSLNVLISSIEMEEGALFVPHYYSKAIVILVVNEGEAHVELVGPKGNKETLEYESYRAEL 294 Query: 126 --GETFVIP 132 + FVIP Sbjct: 295 SKDDVFVIP 303 >4QM9-A nrPDB Length = 173 Score = 29.6 bits (65), Expect = 0.85, Method: Composition-based stats. Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Query: 77 VDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLY-SRVVRAGETFVIPRGL 135 ++ P H H ++ MV++G+LL I S +L S V GE + +GL Sbjct: 76 INIPPNKETTVHDHGQSIGCAMVLEGKLLNSIYRSTGEHAELSNSYFVHEGECLISTKGL 135 Query: 136 MH 137 +H Sbjct: 136 IH 137 >6HD7-E nrPDB Length = 254 Score = 29.2 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 2/31 (6%) Query: 64 WPGTNTLGVSMNRVDFAPGGTNPPHIHPRAT 94 WP T GV+MN VD GG N HI +T Sbjct: 196 WPKTR--GVAMNPVDHPHGGGNHQHIGKAST 224 >5IT7-AA nrPDB Length = 249 Score = 29.2 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 2/31 (6%) Query: 64 WPGTNTLGVSMNRVDFAPGGTNPPHIHPRAT 94 WP T GV+MN VD GG N HI +T Sbjct: 195 WPKTR--GVAMNPVDHPHGGGNHQHIGKAST 223 >3LWA-A nrPDB Length = 183 Score = 28.8 bits (63), Expect = 1.6, Method: Composition-based stats. Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 3/49 (6%) Query: 11 VADLDGKAVSVN--GHTCKPMSEAGDDF-LFSSKLTKAGNTSTPNGSAV 56 ++D + + V +N G C P DD + +L AGN TP G+ + Sbjct: 54 LSDFENQVVILNAWGQWCAPCRSESDDLQIIHEELQAAGNGDTPGGTVL 102 >3WW2-A nrPDB Length = 256 Score = 28.4 bits (62), Expect = 2.1, Method: Composition-based stats. Identities = 13/26 (50%), Positives = 15/26 (57%) Query: 81 PGGTNPPHIHPRATEIGMVMKGELLV 106 PG PPH H R TE V+ GE+ V Sbjct: 106 PGKFCPPHFHQRKTESYEVVLGEMEV 131 >1UN9-A nrPDB Length = 552 Score = 27.6 bits (60), Expect = 3.9, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 29/61 (47%), Gaps = 2/61 (3%) Query: 57 TELDVAEWPGTNT--LGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDS 114 T L A++ +NT LGV+++ P HP E+GM + GE ++ + +S Sbjct: 174 TVLREAQYAASNTFSLGVALSSCHLPQETDAAPRHHPGHAELGMGIHGEPGASVIDTQNS 233 Query: 115 G 115 Sbjct: 234 A 234 >5XXB-A nrPDB Length = 260 Score = 27.6 bits (60), Expect = 4.0, Method: Composition-based stats. Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 3/52 (5%) Query: 64 WPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSG 115 WP GV+MN V+ GG N HI +T M G+ VG++ + +G Sbjct: 196 WPKVR--GVAMNPVEHPHGGGNHQHIGHPSTVSRMAAPGQ-KVGLIAARRTG 244 >4JJP-A nrPDB Length = 267 Score = 27.3 bits (59), Expect = 4.8, Method: Composition-based stats. Identities = 13/31 (41%), Positives = 19/31 (61%) Query: 89 IHPRATEIGMVMKGELLVGILGSLDSGNKLY 119 I PRA +IGMV E+++ I+ +L N Y Sbjct: 76 IPPRAVKIGMVSSPEIILEIVENLKKYNPKY 106 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.141 0.443 Lambda K H 0.267 0.0416 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,836,377 Number of Sequences: 36641 Number of extensions: 365300 Number of successful extensions: 781 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 768 Number of HSP's gapped (non-prelim): 29 length of query: 201 length of database: 10,336,785 effective HSP length: 92 effective length of query: 109 effective length of database: 6,965,813 effective search space: 759273617 effective search space used: 759273617 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 57 (26.5 bits)