BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P40992 (514 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5W66-P nrPDB 1073 0.0 6OAZ-A nrPDB 32 0.61 >5W66-P nrPDB Length = 514 Score = 1073 bits (2774), Expect = 0.0, Method: Compositional matrix adjust. Identities = 514/514 (100%), Positives = 514/514 (100%) Query: 1 MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRL 60 MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRL Sbjct: 1 MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRL 60 Query: 61 NLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLI 120 NLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLI Sbjct: 61 NLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLI 120 Query: 121 TEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCD 180 TEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCD Sbjct: 121 TEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCD 180 Query: 181 YIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFK 240 YIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFK Sbjct: 181 YIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFK 240 Query: 241 EFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSN 300 EFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSN Sbjct: 241 EFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSN 300 Query: 301 HAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDK 360 HAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDK Sbjct: 301 HAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDK 360 Query: 361 PTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLD 420 PTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLD Sbjct: 361 PTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLD 420 Query: 421 REANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLL 480 REANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLL Sbjct: 421 REANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLL 480 Query: 481 THIASQLLVDFAISKEQLKDCISRIKNACLHRMN 514 THIASQLLVDFAISKEQLKDCISRIKNACLHRMN Sbjct: 481 THIASQLLVDFAISKEQLKDCISRIKNACLHRMN 514 >6OAZ-A nrPDB Length = 448 Score = 32.0 bits (71), Expect = 0.61, Method: Compositional matrix adjust. Identities = 31/143 (21%), Positives = 55/143 (38%), Gaps = 20/143 (13%) Query: 310 FMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDKPTSSDYFQW 369 FM+ W ++ + W+ L +L +R S I + + P S+ Y + Sbjct: 49 FMVIHGWTVTGMYE-----SWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKL 103 Query: 370 SEEETLEFLKWMEKQF-LPTQTKSLHNEN-GSMEMTIDQKIARRKLYKIFPLD------- 420 ++ F+ WME++F P L + G+ I + +K+ +I LD Sbjct: 104 VGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFE 163 Query: 421 ------REANHDGEFNDSTHQLT 437 R + D +F D H T Sbjct: 164 YAEAPSRLSPDDADFVDVLHTFT 186 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5W66-P nrPDB 1103 0.0 Sequences not found previously or not previously below threshold: 6OAZ-A nrPDB 31 1.3 4I0N-A nrPDB 29 4.6 CONVERGED! >5W66-P nrPDB Length = 514 Score = 1103 bits (2854), Expect = 0.0, Method: Composition-based stats. Identities = 514/514 (100%), Positives = 514/514 (100%) Query: 1 MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRL 60 MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRL Sbjct: 1 MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDDEDDLNGLGAGVITRRL 60 Query: 61 NLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLI 120 NLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLI Sbjct: 61 NLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKLLFLKSFQFILKRQIRWLI 120 Query: 121 TEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCD 180 TEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCD Sbjct: 121 TEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTSTISILYLASTHLSLPVYTCD 180 Query: 181 YIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFK 240 YIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFK Sbjct: 181 YIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGSISPFNGQLYNKIALTCGMIHFK 240 Query: 241 EFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSN 300 EFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSN Sbjct: 241 EFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEFEETDIRNLTLWERTDERHTGRVSN 300 Query: 301 HAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDK 360 HAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDK Sbjct: 301 HAELRVLSYFMLTINWMLSFDRDRQYPLKWILSLTESLTQRTTTSESIGRNIVKVVYPDK 360 Query: 361 PTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLD 420 PTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLD Sbjct: 361 PTSSDYFQWSEEETLEFLKWMEKQFLPTQTKSLHNENGSMEMTIDQKIARRKLYKIFPLD 420 Query: 421 REANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLL 480 REANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLL Sbjct: 421 REANHDGEFNDSTHQLTFIEDLQERYAKQTPFFESNKIRDSLNYQEANPPARKEAIGRLL 480 Query: 481 THIASQLLVDFAISKEQLKDCISRIKNACLHRMN 514 THIASQLLVDFAISKEQLKDCISRIKNACLHRMN Sbjct: 481 THIASQLLVDFAISKEQLKDCISRIKNACLHRMN 514 >6OAZ-A nrPDB Length = 448 Score = 30.8 bits (68), Expect = 1.3, Method: Composition-based stats. Identities = 28/123 (22%), Positives = 48/123 (39%), Gaps = 15/123 (12%) Query: 330 WILSLTESLTQRTTTSESIGRNIVKVVYPDKPTSSDYFQWSEEETLEFLKWMEKQF-LPT 388 W+ L +L +R S I + + P S+ Y + ++ F+ WME++F P Sbjct: 64 WVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPL 123 Query: 389 QTKSLHNEN-GSMEMTIDQKIARRKLYKIFPLD-------------REANHDGEFNDSTH 434 L + G+ I + +K+ +I LD R + D +F D H Sbjct: 124 DNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLH 183 Query: 435 QLT 437 T Sbjct: 184 TFT 186 >4I0N-A nrPDB Length = 289 Score = 29.2 bits (64), Expect = 4.6, Method: Composition-based stats. Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 3/44 (6%) Query: 273 VIEFEETDIRNLTLWERTDERHTGRVSNHAELRVLSYFMLTINW 316 ++E++ D + WE T R T ++S+ +E + FM INW Sbjct: 240 IVEYQRFDNDYILNWETTQWRGTNKLSSTSE---YNEFMFKINW 280 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.135 0.409 Lambda K H 0.267 0.0428 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 22,177,617 Number of Sequences: 36641 Number of extensions: 951084 Number of successful extensions: 2040 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 2035 Number of HSP's gapped (non-prelim): 9 length of query: 514 length of database: 10,336,785 effective HSP length: 101 effective length of query: 413 effective length of database: 6,636,044 effective search space: 2740686172 effective search space used: 2740686172 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 61 (28.0 bits)