BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P40969 (608 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6F07-A nrPDB 1260 0.0 1QP9-A nrPDB 39 0.006 2WOE-A nrPDB 34 0.17 1PYI-A nrPDB 30 2.8 5N4B-A nrPDB 30 3.3 >6F07-A nrPDB Length = 620 Score = 1260 bits (3261), Expect = 0.0, Method: Compositional matrix adjust. Identities = 608/608 (100%), Positives = 608/608 (100%) Query: 1 MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPD 60 MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPD Sbjct: 1 MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPD 60 Query: 61 LLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAME 120 LLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAME Sbjct: 61 LLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAME 120 Query: 121 NLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLA 180 NLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLA Sbjct: 121 NLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLA 180 Query: 181 EIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL 240 EIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL Sbjct: 181 EIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL 240 Query: 241 ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCD 300 ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCD Sbjct: 241 ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCD 300 Query: 301 YLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLN 360 YLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLN Sbjct: 301 YLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLN 360 Query: 361 KSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLI 420 KSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLI Sbjct: 361 KSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLI 420 Query: 421 YYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA 480 YYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA Sbjct: 421 YYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA 480 Query: 481 AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKI 540 AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKI Sbjct: 481 AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKI 540 Query: 541 LQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSE 600 LQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSE Sbjct: 541 LQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSE 600 Query: 601 YNISDILS 608 YNISDILS Sbjct: 601 YNISDILS 608 >1QP9-A nrPDB Length = 76 Score = 38.9 bits (89), Expect = 0.006, Method: Composition-based stats. Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Query: 14 CSVCTRRKVKCDRMIP-CGNCRKRGQDSECMKSTKLITASSSKEYLPD 60 C++C +RKVKCD++ P C C K G C + + KE L D Sbjct: 10 CTICRKRKVKCDKLRPHCQQCTKTGVAHLCHYMEQTWAEEAEKELLKD 57 >2WOE-A nrPDB Length = 299 Score = 34.3 bits (77), Expect = 0.17, Method: Compositional matrix adjust. Identities = 21/82 (25%), Positives = 36/82 (43%), Gaps = 9/82 (10%) Query: 232 RLVQAYLILATTTFPYDEPLLANSLLTQCIHTFKN--FHVDDFRP-------LLNDDPVE 282 R ++ Y+ TTT PY E N +C+ H D P + ++ P+ Sbjct: 109 RGIRRYMHEGTTTAPYSEGDAGNGAAMRCLPAALATLGHPADLEPWVLAQARITHNHPLS 168 Query: 283 SIAKVTLGRIFYRLCGCDYLQS 304 A +TLGR+ + L G +++ Sbjct: 169 DAACLTLGRMVHHLIGGRGMKA 190 >1PYI-A nrPDB Length = 96 Score = 30.0 bits (66), Expect = 2.8, Method: Composition-based stats. Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Query: 8 LKSKHPCSVCTRRKVKCDRMIP-CGNCRK 35 +KS+ C C +K+KCD+ P C C K Sbjct: 1 MKSRTACKRCRLKKIKCDQEFPSCKRCAK 29 >5N4B-A nrPDB Length = 731 Score = 30.0 bits (66), Expect = 3.3, Method: Compositional matrix adjust. Identities = 38/170 (22%), Positives = 65/170 (38%), Gaps = 27/170 (15%) Query: 38 QDSECMKSTKLITASSSKEYLPDLLLFWQNYEYWITNIGL------YKTKQRDLTRTPAN 91 Q+++ K + AS + L + YW N GL Y++K+ L + Sbjct: 65 QNADIQKLAEKFRASRNYAKFSAPTLLDDGHWYWFYNRGLQSQSVLYRSKEPALPDF-SK 123 Query: 92 LDTDTEECMFWMNYLQKDQSFQLM-----------NFAMENLGALYFGSIGDISELYLRV 140 D + + F N L D S ++ +A+ +LG GD S +Y+R Sbjct: 124 GDDNVGDVFFDPNVLAADGSAGMVLCKFSPDGKFFAYAVSHLG-------GDYSTIYVRS 176 Query: 141 EQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLAEIFSVYPLHE 190 +A VDG+ D + W F+ I+ + ++ YP E Sbjct: 177 TSSPLSQASVAQGVDGRLSDEVKWFKFSTIIWTKDSKGF--LYQRYPARE 224 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6F07-A nrPDB 1291 0.0 Sequences not found previously or not previously below threshold: 1QP9-A nrPDB 40 0.004 1CLD-A nrPDB 33 0.43 1PYI-A nrPDB 30 2.4 5N4B-A nrPDB 30 4.6 4V8P-BF nrPDB 29 6.2 6E33-A nrPDB 29 7.2 1Z6T-A nrPDB 29 7.2 2WOE-A nrPDB 29 7.6 4PQJ-A nrPDB 28 8.2 CONVERGED! >6F07-A nrPDB Length = 620 Score = 1291 bits (3342), Expect = 0.0, Method: Composition-based stats. Identities = 608/608 (100%), Positives = 608/608 (100%) Query: 1 MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPD 60 MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPD Sbjct: 1 MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMKSTKLITASSSKEYLPD 60 Query: 61 LLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAME 120 LLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAME Sbjct: 61 LLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFWMNYLQKDQSFQLMNFAME 120 Query: 121 NLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLA 180 NLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLA Sbjct: 121 NLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWDALIWSVFTMCIYYMPVEKLA 180 Query: 181 EIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL 240 EIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL Sbjct: 181 EIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLFQLKQCDFMAHPDIRLVQAYLIL 240 Query: 241 ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCD 300 ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCD Sbjct: 241 ATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLLNDDPVESIAKVTLGRIFYRLCGCD 300 Query: 301 YLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLN 360 YLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLN Sbjct: 301 YLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLN 360 Query: 361 KSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLI 420 KSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLI Sbjct: 361 KSSKPPLKTLDAIRRELDIFQYKVDSLEEDFRSNNSRFQKFIALFQISTVSWKLFKMYLI 420 Query: 421 YYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA 480 YYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA Sbjct: 421 YYDTADSLLKVIHYSKVIISLIVNNFHAKSEFFNRHPMVMQTITRVVSFISFYQIFVESA 480 Query: 481 AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKI 540 AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKI Sbjct: 481 AVKQLLVDLTELTANLPTIFGSKLDKLVYLTERLSKLKLLWDKVQLLDSGDSFYHPVFKI 540 Query: 541 LQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSE 600 LQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSE Sbjct: 541 LQNDIKIIELKNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSE 600 Query: 601 YNISDILS 608 YNISDILS Sbjct: 601 YNISDILS 608 >1QP9-A nrPDB Length = 76 Score = 39.6 bits (91), Expect = 0.004, Method: Composition-based stats. Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Query: 14 CSVCTRRKVKCDRMIP-CGNCRKRGQDSECMKSTKLITASSSKEYLPD 60 C++C +RKVKCD++ P C C K G C + + KE L D Sbjct: 10 CTICRKRKVKCDKLRPHCQQCTKTGVAHLCHYMEQTWAEEAEKELLKD 57 >1CLD-A nrPDB Length = 61 Score = 32.7 bits (73), Expect = 0.43, Method: Composition-based stats. Identities = 14/49 (28%), Positives = 27/49 (55%), Gaps = 3/49 (6%) Query: 14 CSVCTRRKVKCDRMIP-CGNCRKRGQDSECMKSTKLITASSSKEYLPDL 61 C C ++K KC + +P C NC K D C+ S +++ ++ +L ++ Sbjct: 12 CDACRKKKWKCSKTVPTCTNCLKYNLD--CVYSPQVVRTPLTRAHLTEM 58 >1PYI-A nrPDB Length = 96 Score = 30.4 bits (67), Expect = 2.4, Method: Composition-based stats. Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Query: 8 LKSKHPCSVCTRRKVKCDRMIP-CGNCRK 35 +KS+ C C +K+KCD+ P C C K Sbjct: 1 MKSRTACKRCRLKKIKCDQEFPSCKRCAK 29 >5N4B-A nrPDB Length = 731 Score = 29.6 bits (65), Expect = 4.6, Method: Composition-based stats. Identities = 35/162 (21%), Positives = 60/162 (37%), Gaps = 11/162 (6%) Query: 38 QDSECMKSTKLITASSSKEYLPDLLLFWQNYEYWITNIGLYKTKQRDLTRTPA-----NL 92 Q+++ K + AS + L + YW N GL ++ PA Sbjct: 65 QNADIQKLAEKFRASRNYAKFSAPTLLDDGHWYWFYNRGLQSQSVLYRSKEPALPDFSKG 124 Query: 93 DTDTEECMFWMNYLQKDQSFQLMNFAMENLGALYFGSI----GDISELYLRVEQYWDRRA 148 D + + F N L D S ++ G + ++ GD S +Y+R +A Sbjct: 125 DDNVGDVFFDPNVLAADGSAGMVLCKFSPDGKFFAYAVSHLGGDYSTIYVRSTSSPLSQA 184 Query: 149 DKNHSVDGKYWDALIWSVFTMCIYYMPVEKLAEIFSVYPLHE 190 VDG+ D + W F+ I+ + ++ YP E Sbjct: 185 SVAQGVDGRLSDEVKWFKFSTIIWTKDSKGF--LYQRYPARE 224 >4V8P-BF nrPDB Length = 255 Score = 28.8 bits (63), Expect = 6.2, Method: Composition-based stats. Identities = 19/77 (24%), Positives = 36/77 (46%), Gaps = 9/77 (11%) Query: 540 ILQNDIKIIEL---------KNDEMFSLIKGLGSLVPLNKLRQESLLEEEDENNTEPSDF 590 ++ +D+ IEL KND F+ +KG +L L + + + + N + + Sbjct: 145 VIAHDVDPIELVIFLPQLCRKNDVPFAFVKGKAALGKLVNKKTATAVALTEVRNEDKAKL 204 Query: 591 RTIVEEFQSEYNISDIL 607 + E F++ YN +D L Sbjct: 205 QQFSELFKTNYNANDEL 221 >6E33-A nrPDB Length = 61 Score = 28.8 bits (63), Expect = 7.2, Method: Composition-based stats. Identities = 11/31 (35%), Positives = 15/31 (48%) Query: 9 KSKHPCSVCTRRKVKCDRMIPCGNCRKRGQD 39 ++K C+ C + KCD PC C K D Sbjct: 9 QAKRACAKCQKDNKKCDDARPCQRCIKAKTD 39 >1Z6T-A nrPDB Length = 591 Score = 28.8 bits (63), Expect = 7.2, Method: Composition-based stats. Identities = 9/18 (50%), Positives = 12/18 (66%) Query: 89 PANLDTDTEECMFWMNYL 106 P L D E+CM+W N+L Sbjct: 470 PHTLSPDQEDCMYWYNFL 487 >2WOE-A nrPDB Length = 299 Score = 28.8 bits (63), Expect = 7.6, Method: Composition-based stats. Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 9/76 (11%) Query: 232 RLVQAYLILATTTFPYDEPLLANSLLTQCIHTFKNF--HVDDFRP-------LLNDDPVE 282 R ++ Y+ TTT PY E N +C+ H D P + ++ P+ Sbjct: 109 RGIRRYMHEGTTTAPYSEGDAGNGAAMRCLPAALATLGHPADLEPWVLAQARITHNHPLS 168 Query: 283 SIAKVTLGRIFYRLCG 298 A +TLGR+ + L G Sbjct: 169 DAACLTLGRMVHHLIG 184 >4PQJ-A nrPDB Length = 299 Score = 28.5 bits (62), Expect = 8.2, Method: Composition-based stats. Identities = 31/107 (28%), Positives = 51/107 (47%), Gaps = 25/107 (23%) Query: 271 DFRPLLNDDP-----VESIAKVTLGRIFYRLCGCDYLQSGPRKPIALHTEVSSLLQHAAY 325 DFRP +N+ P V+S+++ +L I Y G ++ K +AL + + AA+ Sbjct: 176 DFRPNVNEQPGSASVVQSVSQ-SLNGIGYSGIG---YKTASVKTVALAKK-----EGAAF 226 Query: 326 LQDLPNVDVYREENSTEVLYWKIISLDRDLDQYLNKSSKPPLKTLDA 372 ++D E+N+ Y L R L Y+NK+ PL L+A Sbjct: 227 VED-------NEQNALNGTY----PLSRFLYVYVNKAPNKPLDPLEA 262 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.137 0.415 Lambda K H 0.267 0.0442 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 27,624,071 Number of Sequences: 36641 Number of extensions: 1275425 Number of successful extensions: 2919 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 20 Number of HSP's that attempted gapping in prelim test: 2911 Number of HSP's gapped (non-prelim): 28 length of query: 608 length of database: 10,336,785 effective HSP length: 103 effective length of query: 505 effective length of database: 6,562,762 effective search space: 3314194810 effective search space used: 3314194810 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 62 (28.4 bits)