BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= P40710
         (236 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2Z4I-A nrPDB                                                          452   e-128
4U3Q-A nrPDB                                                           45   2e-05
5HM3-A nrPDB                                                           28   4.1  
2VUN-A nrPDB                                                           27   6.0  
5LFK-A nrPDB                                                           27   8.6  

>2Z4I-A nrPDB
          Length = 233

 Score =  452 bits (1162), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 215/215 (100%), Positives = 215/215 (100%)

Query: 22  NNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREE 81
           NNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREE
Sbjct: 2   NNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREE 61

Query: 82  PSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSS 141
           PSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSS
Sbjct: 62  PSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSS 121

Query: 142 LPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGH 201
           LPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGH
Sbjct: 122 LPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGH 181

Query: 202 FTLEGNPDTGAPTKVLAPDTAGKFYPNQDCSSLGQ 236
           FTLEGNPDTGAPTKVLAPDTAGKFYPNQDCSSLGQ
Sbjct: 182 FTLEGNPDTGAPTKVLAPDTAGKFYPNQDCSSLGQ 216


>4U3Q-A nrPDB
          Length = 122

 Score = 45.1 bits (105), Expect = 2e-05,   Method: Compositional matrix adjust.
 Identities = 27/78 (34%), Positives = 39/78 (50%), Gaps = 1/78 (1%)

Query: 45  WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLV-LTDS 103
           +RG LP ADC GI+T++    DGT    E  L  +  PS     GTW    D +V L+  
Sbjct: 17  FRGTLPAADCPGIDTTVTFNADGTAQKVELALEKKSAPSPLTYRGTWMVREDGIVELSLV 76

Query: 104 KGEKSYYRAKGDALEMLD 121
             E+S    + +  E++D
Sbjct: 77  SSEQSKAPHEKELYELID 94


>5HM3-A nrPDB
          Length = 657

 Score = 27.7 bits (60), Expect = 4.1,   Method: Compositional matrix adjust.
 Identities = 12/40 (30%), Positives = 21/40 (52%)

Query: 160 ATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVE 199
           A   DCA        ++AE  R ++ AR   E+P +++V+
Sbjct: 149 AVLDDCAPSTILTTTDSAEGVRKFIRARSAKERPRVIAVD 188


>2VUN-A nrPDB
          Length = 386

 Score = 27.3 bits (59), Expect = 6.0,   Method: Compositional matrix adjust.
 Identities = 11/36 (30%), Positives = 18/36 (50%)

Query: 58  ETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWAR 93
           E  + ++K+G W++ E  LG  + P   A    WA 
Sbjct: 149 EDFIEMKKEGVWIVGEVGLGTIKNPEDAAPMVEWAH 184


>5LFK-A nrPDB
          Length = 298

 Score = 26.9 bits (58), Expect = 8.6,   Method: Compositional matrix adjust.
 Identities = 18/67 (26%), Positives = 27/67 (40%)

Query: 113 KGDALEMLDREGNPIESQFNYTLEAAQSSLPMTPMTLRGMYFYMADAATFTDCATGKRFM 172
           +G  +E L  +G P     N   E AQ +L   P    G   ++A  A+F    T    M
Sbjct: 17  RGSHMENLYFQGKPARKLKNAADEVAQGNLRQHPELEAGPQEFLAAGASFNQVVTALERM 76

Query: 173 VANNAEL 179
           + +   L
Sbjct: 77  MTSQQRL 83


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

2Z4I-A nrPDB                                                          439   e-124
4U3Q-A nrPDB                                                          130   6e-31

Sequences not found previously or not previously below threshold:

3E53-A nrPDB                                                           30   1.3  
5HM3-A nrPDB                                                           29   1.7  
5IRR-A nrPDB                                                           28   3.2  
5Z7D-A nrPDB                                                           27   9.7  
5YZW-A nrPDB                                                           27   9.7  


CONVERGED!
>2Z4I-A nrPDB
          Length = 233

 Score =  439 bits (1130), Expect = e-124,   Method: Composition-based stats.
 Identities = 215/215 (100%), Positives = 215/215 (100%)

Query: 22  NNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREE 81
           NNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREE
Sbjct: 2   NNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREE 61

Query: 82  PSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSS 141
           PSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSS
Sbjct: 62  PSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSS 121

Query: 142 LPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGH 201
           LPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGH
Sbjct: 122 LPMTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGH 181

Query: 202 FTLEGNPDTGAPTKVLAPDTAGKFYPNQDCSSLGQ 236
           FTLEGNPDTGAPTKVLAPDTAGKFYPNQDCSSLGQ
Sbjct: 182 FTLEGNPDTGAPTKVLAPDTAGKFYPNQDCSSLGQ 216


>4U3Q-A nrPDB
          Length = 122

 Score =  130 bits (326), Expect = 6e-31,   Method: Composition-based stats.
 Identities = 27/78 (34%), Positives = 39/78 (50%), Gaps = 1/78 (1%)

Query: 45  WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLV-LTDS 103
           +RG LP ADC GI+T++    DGT    E  L  +  PS     GTW    D +V L+  
Sbjct: 17  FRGTLPAADCPGIDTTVTFNADGTAQKVELALEKKSAPSPLTYRGTWMVREDGIVELSLV 76

Query: 104 KGEKSYYRAKGDALEMLD 121
             E+S    + +  E++D
Sbjct: 77  SSEQSKAPHEKELYELID 94


>3E53-A nrPDB
          Length = 480

 Score = 29.7 bits (65), Expect = 1.3,   Method: Composition-based stats.
 Identities = 19/63 (30%), Positives = 26/63 (41%), Gaps = 9/63 (14%)

Query: 51  CADCE-GIETSLFLEKD----GTWVM---NERYLGAR-EEPSSFASYGTWARTADKLVLT 101
           C +C  G    +++  D    G W     +ER  G +   PS     G W RT D   +T
Sbjct: 412 CIECPDGTVGEIWVHGDNVANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFVT 471

Query: 102 DSK 104
           D K
Sbjct: 472 DGK 474


>5HM3-A nrPDB
          Length = 657

 Score = 28.9 bits (63), Expect = 1.7,   Method: Composition-based stats.
 Identities = 12/41 (29%), Positives = 21/41 (51%)

Query: 160 ATFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEG 200
           A   DCA        ++AE  R ++ AR   E+P +++V+ 
Sbjct: 149 AVLDDCAPSTILTTTDSAEGVRKFIRARSAKERPRVIAVDA 189


>5IRR-A nrPDB
          Length = 308

 Score = 28.2 bits (61), Expect = 3.2,   Method: Composition-based stats.
 Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 3/75 (4%)

Query: 66  DGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGN 125
           DG++   E++   R +P S +S  +W    D+++      +   Y  + D    L    +
Sbjct: 58  DGSYTPTEQF---RRDPESLSSTVSWRDEEDRVIWVYKIQDTPGYGDELDVFRNLKMVQD 114

Query: 126 PIESQFNYTLEAAQS 140
            IESQ    LE  Q+
Sbjct: 115 YIESQNRKWLELEQA 129


>5Z7D-A nrPDB
          Length = 412

 Score = 26.6 bits (57), Expect = 9.7,   Method: Composition-based stats.
 Identities = 18/78 (23%), Positives = 31/78 (39%)

Query: 12  VISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVM 71
           +I++    GCN   E+   S      + P       +   A+     + LF +  GT+V 
Sbjct: 273 IIAISDYFGCNGFLEIYRASCVSDVNVNPTMVISNTLRQRANATPKISYLFSQARGTFVS 332

Query: 72  NERYLGAREEPSSFASYG 89
            E  +  + E + F  YG
Sbjct: 333 GEYLVNKKTERNKFIYYG 350


>5YZW-A nrPDB
          Length = 201

 Score = 26.6 bits (57), Expect = 9.7,   Method: Composition-based stats.
 Identities = 18/78 (23%), Positives = 31/78 (39%)

Query: 12  VISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVM 71
           +I++    GCN   E+   S      + P       +   A+     + LF +  GT+V 
Sbjct: 62  IIAISDYFGCNGFLEIYRASCVSDVNVNPTMVISNTLRQRANATPKISYLFSQARGTFVS 121

Query: 72  NERYLGAREEPSSFASYG 89
            E  +  + E + F  YG
Sbjct: 122 GEYLVNKKTERNKFIYYG 139


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.312    0.128    0.364 

Lambda     K      H
   0.267   0.0400    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,906,804
Number of Sequences: 36641
Number of extensions: 336182
Number of successful extensions: 789
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 780
Number of HSP's gapped (non-prelim): 13
length of query: 236
length of database: 10,336,785
effective HSP length: 94
effective length of query: 142
effective length of database: 6,892,531
effective search space: 978739402
effective search space used: 978739402
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 57 (26.6 bits)