BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P40337 (213 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4WQO-A nrPDB 432 e-122 6DXU-D nrPDB 31 0.41 3WEN-A nrPDB 30 0.88 2R8T-A nrPDB 30 1.1 4KCB-A nrPDB 28 2.6 6F8L-G nrPDB 28 2.8 5L0V-A nrPDB 27 7.0 >4WQO-A nrPDB Length = 233 Score = 432 bits (1110), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/213 (100%), Positives = 213/213 (100%) Query: 1 MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR 60 MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR Sbjct: 21 MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR 80 Query: 61 PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR 120 PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR Sbjct: 81 PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR 140 Query: 121 DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI 180 DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI Sbjct: 141 DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI 200 Query: 181 VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD 213 VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD Sbjct: 201 VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD 233 >6DXU-D nrPDB Length = 830 Score = 30.8 bits (68), Expect = 0.41, Method: Compositional matrix adjust. Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Query: 158 LKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLE 199 LKE L+V S PE+ R+ D+ S+Y D++ P K LE Sbjct: 217 LKEGILKVKTSFYNPEDIRKADVTVSVY-DVDRKPVFSKTLE 257 >3WEN-A nrPDB Length = 913 Score = 29.6 bits (65), Expect = 0.88, Method: Composition-based stats. Identities = 27/100 (27%), Positives = 41/100 (41%), Gaps = 12/100 (12%) Query: 75 IFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTE 134 IF R+P +VL + G P P P G H R W +RD + ++ E Sbjct: 294 IFAGRTPEMVLDQYTKLIGRPAPMPYWAFG----FHQCR---WGYRDVNEIETVVDKYAE 346 Query: 135 LFVPS----LNVDGQPIFANITL-PVYTLKERCLQVVRSL 169 +P ++D F + TL PV+ ++ Q V L Sbjct: 347 ARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKL 386 >2R8T-A nrPDB Length = 338 Score = 29.6 bits (65), Expect = 1.1, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Query: 111 SYRGHLWLFR-DAGTHDGLLVNQTELFVPSLNVDGQPIF 148 + G+ WL R D T DG VN + + +N+DG + Sbjct: 19 ALAGYKWLGRGDKNTADGAAVNAMRIMLNQVNIDGTIVI 57 >4KCB-A nrPDB Length = 447 Score = 28.1 bits (61), Expect = 2.6, Method: Compositional matrix adjust. Identities = 22/85 (25%), Positives = 34/85 (40%), Gaps = 6/85 (7%) Query: 99 PTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITL----P 154 P +P + YRGH W D H+G N + N TL P Sbjct: 172 PEIPQWAMEAVPGYRGHTWA-PDISEHNGTWYMYYSCSTFGKNGSAIGLMTNKTLNPESP 230 Query: 155 VYTLKERCLQVVRSLVKPENYRRLD 179 Y +++ + VVRS+ + N+ +D Sbjct: 231 DYKWEDKGM-VVRSVQRQTNWNAID 254 >6F8L-G nrPDB Length = 913 Score = 28.1 bits (61), Expect = 2.8, Method: Compositional matrix adjust. Identities = 29/110 (26%), Positives = 43/110 (39%), Gaps = 7/110 (6%) Query: 1 MPRRAENWDEAEVGAEEAGVEE-----YGPEEDGGEESGAEESGPEESGPEELGAEEEME 55 + R+ N++ A A EA + + YG E + EE P L +E Sbjct: 448 LKRKGLNYEVAPAVATEAAITKLIERFYGKAELSEIAKEFAKKQAEEEVPSPLELDESAA 507 Query: 56 AGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGT 105 + V+R ++ S + R V V L DG +PY TLP G Sbjct: 508 QKFVKQVIREAFLQDASDIHIEPRQNDV--QVRLRIDGALRPYSTLPKGA 555 >5L0V-A nrPDB Length = 357 Score = 26.9 bits (58), Expect = 7.0, Method: Compositional matrix adjust. Identities = 9/25 (36%), Positives = 14/25 (56%) Query: 83 VVLPVWLNFDGEPQPYPTLPPGTGR 107 ++ P W ++G P +P P G GR Sbjct: 131 IMYPAWTFWEGGPAVWPIYPTGLGR 155 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4WQO-A nrPDB 443 e-125 Sequences not found previously or not previously below threshold: 6DXU-D nrPDB 32 0.22 3WEN-A nrPDB 32 0.25 6F8L-G nrPDB 31 0.31 5L3D-A nrPDB 31 0.47 3D8B-A nrPDB 30 0.81 3J7Y-F nrPDB 30 1.1 3TQD-A nrPDB 28 2.0 2R8T-A nrPDB 28 3.1 6NU3-K nrPDB 28 3.7 5INF-A nrPDB 28 3.9 4KCB-A nrPDB 27 6.2 CONVERGED! >4WQO-A nrPDB Length = 233 Score = 443 bits (1140), Expect = e-125, Method: Composition-based stats. Identities = 213/213 (100%), Positives = 213/213 (100%) Query: 1 MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR 60 MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR Sbjct: 21 MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR 80 Query: 61 PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR 120 PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR Sbjct: 81 PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR 140 Query: 121 DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI 180 DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI Sbjct: 141 DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI 200 Query: 181 VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD 213 VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD Sbjct: 201 VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD 233 >6DXU-D nrPDB Length = 830 Score = 31.9 bits (71), Expect = 0.22, Method: Composition-based stats. Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Query: 158 LKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLE 199 LKE L+V S PE+ R+ D+ S+Y D++ P K LE Sbjct: 217 LKEGILKVKTSFYNPEDIRKADVTVSVY-DVDRKPVFSKTLE 257 >3WEN-A nrPDB Length = 913 Score = 31.5 bits (70), Expect = 0.25, Method: Composition-based stats. Identities = 27/100 (27%), Positives = 41/100 (41%), Gaps = 12/100 (12%) Query: 75 IFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTE 134 IF R+P +VL + G P P P G H R W +RD + ++ E Sbjct: 294 IFAGRTPEMVLDQYTKLIGRPAPMPYWAFG----FHQCR---WGYRDVNEIETVVDKYAE 346 Query: 135 LFVPS----LNVDGQPIFANITL-PVYTLKERCLQVVRSL 169 +P ++D F + TL PV+ ++ Q V L Sbjct: 347 ARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKL 386 >6F8L-G nrPDB Length = 913 Score = 31.2 bits (69), Expect = 0.31, Method: Composition-based stats. Identities = 29/109 (26%), Positives = 43/109 (39%), Gaps = 7/109 (6%) Query: 1 MPRRAENWDEAEVGAEEAGVEE-----YGPEEDGGEESGAEESGPEESGPEELGAEEEME 55 + R+ N++ A A EA + + YG E + EE P L +E Sbjct: 448 LKRKGLNYEVAPAVATEAAITKLIERFYGKAELSEIAKEFAKKQAEEEVPSPLELDESAA 507 Query: 56 AGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPG 104 + V+R ++ S + R V V L DG +PY TLP G Sbjct: 508 QKFVKQVIREAFLQDASDIHIEPRQNDV--QVRLRIDGALRPYSTLPKG 554 >5L3D-A nrPDB Length = 852 Score = 30.8 bits (68), Expect = 0.47, Method: Composition-based stats. Identities = 28/95 (29%), Positives = 35/95 (36%), Gaps = 32/95 (33%) Query: 22 EYGPEEDGGEESGAEE-------SGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQV 74 E GP GG E+G+E SGP E GP +G R+ +EP Sbjct: 22 EAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGE-------------RTPRKKEP--- 65 Query: 75 IFCNRSPRVVLPVWLN---FDGEPQPYPTLPPGTG 106 PR P L PQ PT+ PG+ Sbjct: 66 ------PRASPPGGLAEPPGSAGPQAGPTVVPGSA 94 >3D8B-A nrPDB Length = 357 Score = 30.0 bits (66), Expect = 0.81, Method: Composition-based stats. Identities = 29/111 (26%), Positives = 45/111 (40%), Gaps = 32/111 (28%) Query: 25 PEEDGGEESGAEESGPEESGPE------------------ELGAEEEMEAGRPRPVLRSV 66 P++DGGE++G + P +GP EL E M+ G P V Sbjct: 28 PKQDGGEQNGGMQCKPYGAGPTEPAHPVDERLKNLEPKMIELIMNEIMDHGPP------V 81 Query: 67 NSREPSQVIFCNRSPR--VVLP-----VWLNFDGEPQPYPTL-PPGTGRRI 109 N + + V F + + VV P ++ G P+ PPGTG+ + Sbjct: 82 NWEDIAGVEFAKATIKEIVVWPMLRPDIFTGLRGPPKGILLFGPPGTGKTL 132 >3J7Y-F nrPDB Length = 311 Score = 29.6 bits (65), Expect = 1.1, Method: Composition-based stats. Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 5/49 (10%) Query: 25 PEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQ 73 P G S AEE+ PE++ +E G P PVLR V P+ Sbjct: 15 PTGSQGLSSLAEEAARATENPEQVASE-----GLPEPVLRKVELPVPTH 58 >3TQD-A nrPDB Length = 256 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 6/34 (17%) Query: 162 CLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQ 195 CLQ L+ P D +R L EDL++H NV+ Sbjct: 103 CLQGDEPLIPP------DAIRKLAEDLDEHDNVK 130 >2R8T-A nrPDB Length = 338 Score = 28.1 bits (61), Expect = 3.1, Method: Composition-based stats. Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Query: 114 GHLWLFR-DAGTHDGLLVNQTELFVPSLNVDG 144 G+ WL R D T DG VN + + +N+DG Sbjct: 22 GYKWLGRGDKNTADGAAVNAMRIMLNQVNIDG 53 >6NU3-K nrPDB Length = 178 Score = 27.7 bits (60), Expect = 3.7, Method: Composition-based stats. Identities = 13/35 (37%), Positives = 20/35 (57%) Query: 172 PENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERI 206 P+ Y DI+++L E+L + K L+ TQE I Sbjct: 129 PDEYIPEDILKNLVEELPQPRKIPKRLDEYTQEEI 163 >5INF-A nrPDB Length = 538 Score = 27.7 bits (60), Expect = 3.9, Method: Composition-based stats. Identities = 15/66 (22%), Positives = 33/66 (50%), Gaps = 3/66 (4%) Query: 136 FVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYED---LEDHP 192 ++P N+D P++ + P T + L V + + Y D++ ++ +D LE HP Sbjct: 264 YLPPNNLDPAPVYDHDCAPGITEADLALDTVIPDSEQQVYDMADVITAVLDDGDYLEIHP 323 Query: 193 NVQKDL 198 + +++ Sbjct: 324 DFARNI 329 >4KCB-A nrPDB Length = 447 Score = 26.9 bits (58), Expect = 6.2, Method: Composition-based stats. Identities = 22/85 (25%), Positives = 34/85 (40%), Gaps = 6/85 (7%) Query: 99 PTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITL----P 154 P +P + YRGH W D H+G N + N TL P Sbjct: 172 PEIPQWAMEAVPGYRGHTWA-PDISEHNGTWYMYYSCSTFGKNGSAIGLMTNKTLNPESP 230 Query: 155 VYTLKERCLQVVRSLVKPENYRRLD 179 Y +++ + VVRS+ + N+ +D Sbjct: 231 DYKWEDKGM-VVRSVQRQTNWNAID 254 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.136 0.410 Lambda K H 0.267 0.0419 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,877,457 Number of Sequences: 36641 Number of extensions: 459149 Number of successful extensions: 880 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 21 Number of HSP's successfully gapped in prelim test: 24 Number of HSP's that attempted gapping in prelim test: 799 Number of HSP's gapped (non-prelim): 81 length of query: 213 length of database: 10,336,785 effective HSP length: 93 effective length of query: 120 effective length of database: 6,929,172 effective search space: 831500640 effective search space used: 831500640 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 57 (26.5 bits)