BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P39742 (193 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6N3Q-F nrPDB 393 e-110 2C2L-A nrPDB 29 1.1 5L0M-A nrPDB 28 2.0 4JIS-A nrPDB 27 4.4 6C66-I nrPDB 27 6.3 5I6E-A nrPDB 26 9.3 4CGU-A nrPDB 26 9.6 >6N3Q-F nrPDB Length = 193 Score = 393 bits (1009), Expect = e-110, Method: Compositional matrix adjust. Identities = 193/193 (100%), Positives = 193/193 (100%) Query: 1 MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKG 60 MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKG Sbjct: 1 MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKG 60 Query: 61 LFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILG 120 LFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILG Sbjct: 61 LFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILG 120 Query: 121 KHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALL 180 KHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALL Sbjct: 121 KHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALL 180 Query: 181 IETARNLAEYNGE 193 IETARNLAEYNGE Sbjct: 181 IETARNLAEYNGE 193 >2C2L-A nrPDB Length = 281 Score = 29.3 bits (64), Expect = 1.1, Method: Compositional matrix adjust. Identities = 15/50 (30%), Positives = 25/50 (50%) Query: 142 VFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALLIETARNLAEYN 191 + +A C LK++Q E+A A C R L L + +K L + + Y+ Sbjct: 40 YYTNRALCYLKMQQPEQALADCRRALELDGQSVKAHFFLGQCQLEMESYD 89 >5L0M-A nrPDB Length = 112 Score = 28.5 bits (62), Expect = 2.0, Method: Compositional matrix adjust. Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 18/70 (25%) Query: 58 IKGLFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCL 117 KG F+ + +Q K ++N + I+ QRKR P+ + CL Sbjct: 31 CKGFFK---RTVQNNKRYTCIENQNCQIDKTQRKRCPY---------------CRFQKCL 72 Query: 118 ILGKHLEALQ 127 +G LEA++ Sbjct: 73 SVGMKLEAVR 82 >4JIS-A nrPDB Length = 247 Score = 27.3 bits (59), Expect = 4.4, Method: Compositional matrix adjust. Identities = 27/100 (27%), Positives = 47/100 (47%), Gaps = 10/100 (10%) Query: 3 TLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKGLF 62 L+Y A +I+A D ++A + I I V G+T +F +SK+++ Sbjct: 131 VLQYGAVDTVISAIDTIIASEDQEFISDIPVRDNMYQGQTPQSFR------ISKLVE--L 182 Query: 63 ESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQL 102 + + + Q+ L +A K SLA E + R E F I++ Sbjct: 183 YNKLSDEQKAVLTDACKICSLAGEKVKLVRG--EVFNIKV 220 >6C66-I nrPDB Length = 244 Score = 26.6 bits (57), Expect = 6.3, Method: Compositional matrix adjust. Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 5/41 (12%) Query: 141 DVFVRKADCLLK-----LRQWEEARATCERGLALAPEDMKL 176 D ++ AD L+K + ARA RG+ LAPED ++ Sbjct: 4 DYILQHADALVKRVSKLIVNEPAARAALRRGVGLAPEDPRM 44 >5I6E-A nrPDB Length = 728 Score = 26.2 bits (56), Expect = 9.3, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Query: 64 SGMKNLQQKKLNEALKNVSLA--IEMAQRKRAPWEAFAIQLPELHFMLRSKID 114 S ++N+ + N+ + N SL IE+ + + P+ + + + LH L +K+D Sbjct: 41 STLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKLHISALHSRLPAKLD 93 >4CGU-A nrPDB Length = 112 Score = 26.2 bits (56), Expect = 9.6, Method: Compositional matrix adjust. Identities = 15/48 (31%), Positives = 25/48 (52%) Query: 120 GKHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGL 167 G + EA+ D L+ P + KA L+KL ++ +A C++GL Sbjct: 19 GLYREAVHCYDQLITAQPQNPVGYSNKAMALIKLGEYTQAIQMCQQGL 66 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6N3Q-F nrPDB 390 e-109 Sequences not found previously or not previously below threshold: 2C2L-A nrPDB 32 0.25 4JIS-A nrPDB 29 1.3 6C66-I nrPDB 29 1.9 4WEA-A nrPDB 28 3.3 5OMP-A nrPDB 27 4.0 1KT1-A nrPDB 27 4.0 4LGB-B nrPDB 27 4.1 5I6E-A nrPDB 27 5.1 5CSA-A nrPDB 27 5.1 5CS0-A nrPDB 27 5.1 3ISL-A nrPDB 27 5.4 1UW4-B nrPDB 27 5.6 1IHG-A nrPDB 27 6.0 5L0M-A nrPDB 27 6.1 4BJ6-A nrPDB 26 8.8 CONVERGED! >6N3Q-F nrPDB Length = 193 Score = 390 bits (1001), Expect = e-109, Method: Composition-based stats. Identities = 193/193 (100%), Positives = 193/193 (100%) Query: 1 MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKG 60 MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKG Sbjct: 1 MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKG 60 Query: 61 LFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILG 120 LFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILG Sbjct: 61 LFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLILG 120 Query: 121 KHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALL 180 KHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALL Sbjct: 121 KHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALL 180 Query: 181 IETARNLAEYNGE 193 IETARNLAEYNGE Sbjct: 181 IETARNLAEYNGE 193 >2C2L-A nrPDB Length = 281 Score = 31.6 bits (70), Expect = 0.25, Method: Composition-based stats. Identities = 15/49 (30%), Positives = 25/49 (51%) Query: 143 FVRKADCLLKLRQWEEARATCERGLALAPEDMKLRALLIETARNLAEYN 191 + +A C LK++Q E+A A C R L L + +K L + + Y+ Sbjct: 41 YTNRALCYLKMQQPEQALADCRRALELDGQSVKAHFFLGQCQLEMESYD 89 >4JIS-A nrPDB Length = 247 Score = 28.9 bits (63), Expect = 1.3, Method: Composition-based stats. Identities = 27/99 (27%), Positives = 47/99 (47%), Gaps = 10/99 (10%) Query: 4 LEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNPNFTPQPNEALSKMIKGLFE 63 L+Y A +I+A D ++A + I I V G+T +F +SK+++ Sbjct: 132 LQYGAVDTVISAIDTIIASEDQEFISDIPVRDNMYQGQTPQSFR------ISKLVE--LY 183 Query: 64 SGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQL 102 + + + Q+ L +A K SLA E + R E F I++ Sbjct: 184 NKLSDEQKAVLTDACKICSLAGEKVKLVRG--EVFNIKV 220 >6C66-I nrPDB Length = 244 Score = 28.5 bits (62), Expect = 1.9, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 5/46 (10%) Query: 138 IQPDVFVRKADCLLK-----LRQWEEARATCERGLALAPEDMKLRA 178 + D ++ AD L+K + ARA RG+ LAPED ++ A Sbjct: 1 MNSDYILQHADALVKRVSKLIVNEPAARAALRRGVGLAPEDPRMLA 46 >4WEA-A nrPDB Length = 515 Score = 27.7 bits (60), Expect = 3.3, Method: Composition-based stats. Identities = 13/24 (54%), Positives = 15/24 (62%) Query: 23 STETNIDQINVLTTSLIGETNPNF 46 ST+ IDQIN LIG+TN F Sbjct: 374 STQAAIDQINGKLNRLIGKTNEKF 397 >5OMP-A nrPDB Length = 462 Score = 27.3 bits (59), Expect = 4.0, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 19/33 (57%) Query: 143 FVRKADCLLKLRQWEEARATCERGLALAPEDMK 175 F+ A C LKLR++ +A C++ L L + K Sbjct: 325 FLNLAMCYLKLREYTKAVECCDKALGLDSANEK 357 >1KT1-A nrPDB Length = 457 Score = 27.3 bits (59), Expect = 4.0, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 19/33 (57%) Query: 143 FVRKADCLLKLRQWEEARATCERGLALAPEDMK 175 F+ A C LKLR++ +A C++ L L + K Sbjct: 320 FLNLAMCYLKLREYTKAVECCDKALGLDSANEK 352 Score = 26.2 bits (56), Expect = 8.2, Method: Composition-based stats. Identities = 19/88 (21%), Positives = 41/88 (46%), Gaps = 3/88 (3%) Query: 107 FMLRSKIDLC---LILGKHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLRQWEEARATC 163 F+L + ++L L L ++ +A++ D LG R+ + L + ++E A+ Sbjct: 315 FLLAAFLNLAMCYLKLREYTKAVECCDKALGLDSANEKGLYRRGEAQLLMNEFESAKGDF 374 Query: 164 ERGLALAPEDMKLRALLIETARNLAEYN 191 E+ L + P++ R + + E+N Sbjct: 375 EKVLEVNPQNKAARLQIFMCQKKAKEHN 402 >4LGB-B nrPDB Length = 341 Score = 27.3 bits (59), Expect = 4.1, Method: Composition-based stats. Identities = 12/23 (52%), Positives = 15/23 (65%) Query: 98 FAIQLPELHFMLRSKIDLCLILG 120 + I PE+ FM RS+ D CLIL Sbjct: 238 YVIPEPEVTFMPRSREDECLILA 260 >5I6E-A nrPDB Length = 728 Score = 27.0 bits (58), Expect = 5.1, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Query: 64 SGMKNLQQKKLNEALKNVSLA--IEMAQRKRAPWEAFAIQLPELHFMLRSKID 114 S ++N+ + N+ + N SL IE+ + + P+ + + + LH L +K+D Sbjct: 41 STLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKLHISALHSRLPAKLD 93 >5CSA-A nrPDB Length = 932 Score = 27.0 bits (58), Expect = 5.1, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Query: 64 SGMKNLQQKKLNEALKNVSLA--IEMAQRKRAPWEAFAIQLPELHFMLRSKID 114 S ++N+ + N+ + N SL IE+ + + P+ + + + LH L +K+D Sbjct: 239 STLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKLHISALHSRLPAKLD 291 >5CS0-A nrPDB Length = 243 Score = 27.0 bits (58), Expect = 5.1, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Query: 64 SGMKNLQQKKLNEALKNVSLA--IEMAQRKRAPWEAFAIQLPELHFMLRSKID 114 S ++N+ + N+ + N SL IE+ + + P+ + + + LH L +K+D Sbjct: 11 STLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKLHISALHSRLPAKLD 63 >3ISL-A nrPDB Length = 416 Score = 27.0 bits (58), Expect = 5.4, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 24/34 (70%), Gaps = 1/34 (2%) Query: 31 INVLTTSLIGETNPNFTPQPNEALSKMIKGLFES 64 + V++T ++G+ +P FT NE + +M++ LF++ Sbjct: 27 LRVMSTPVVGQFDPAFTGIMNETM-EMLRELFQT 59 >1UW4-B nrPDB Length = 248 Score = 27.0 bits (58), Expect = 5.6, Method: Composition-based stats. Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 2/33 (6%) Query: 108 MLRSKIDLCLILGKHLEALQDL--DFLLGTGLI 138 +LR KI LC L + + +QDL +FL+ GL+ Sbjct: 215 LLRPKIKLCNSLEESIRQVQDLEREFLIKLGLV 247 >1IHG-A nrPDB Length = 370 Score = 27.0 bits (58), Expect = 6.0, Method: Composition-based stats. Identities = 11/43 (25%), Positives = 26/43 (60%) Query: 145 RKADCLLKLRQWEEARATCERGLALAPEDMKLRALLIETARNL 187 R+A L+++++A A ++ +APED ++A L++ + + Sbjct: 312 RRAQGWQGLKEYDQALADLKKAQEIAPEDKAIQAELLKVKQKI 354 >5L0M-A nrPDB Length = 112 Score = 27.0 bits (58), Expect = 6.1, Method: Composition-based stats. Identities = 18/69 (26%), Positives = 30/69 (43%), Gaps = 18/69 (26%) Query: 59 KGLFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQLPELHFMLRSKIDLCLI 118 KG F+ + +Q K ++N + I+ QRKR P+ + CL Sbjct: 32 KGFFK---RTVQNNKRYTCIENQNCQIDKTQRKRCPY---------------CRFQKCLS 73 Query: 119 LGKHLEALQ 127 +G LEA++ Sbjct: 74 VGMKLEAVR 82 >4BJ6-A nrPDB Length = 365 Score = 26.2 bits (56), Expect = 8.8, Method: Composition-based stats. Identities = 17/44 (38%), Positives = 22/44 (50%), Gaps = 6/44 (13%) Query: 49 QPNEALSKMIKGLFESGMKNLQQK------KLNEALKNVSLAIE 86 Q E L KMIK F+SG K LQ +LN N+++ E Sbjct: 256 QEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFE 299 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.133 0.373 Lambda K H 0.267 0.0428 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,369,222 Number of Sequences: 36641 Number of extensions: 283092 Number of successful extensions: 895 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 870 Number of HSP's gapped (non-prelim): 43 length of query: 193 length of database: 10,336,785 effective HSP length: 92 effective length of query: 101 effective length of database: 6,965,813 effective search space: 703547113 effective search space used: 703547113 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.1 bits)