BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38907 (458 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6NUW-E nrPDB 941 0.0 4JE3-B nrPDB 201 6e-52 5D1Y-A nrPDB 30 2.1 2WBK-A nrPDB 28 7.2 2E5V-A nrPDB 28 8.4 3CZQ-A nrPDB 28 9.4 >6NUW-E nrPDB Length = 461 Score = 941 bits (2433), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/458 (100%), Positives = 458/458 (100%) Query: 1 MSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTETLDL 60 MSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTETLDL Sbjct: 4 MSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTETLDL 63 Query: 61 LIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGKPYV 120 LIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGKPYV Sbjct: 64 LIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGKPYV 123 Query: 121 VKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKE 180 VKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKE Sbjct: 124 VKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKE 183 Query: 181 SLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERETIIF 240 SLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERETIIF Sbjct: 184 SLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERETIIF 243 Query: 241 KPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKVMVR 300 KPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKVMVR Sbjct: 244 KPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKVMVR 303 Query: 301 EFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHNIRN 360 EFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHNIRN Sbjct: 304 EFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHNIRN 363 Query: 361 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 420 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN Sbjct: 364 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 423 Query: 421 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 458 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL Sbjct: 424 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 461 >4JE3-B nrPDB Length = 101 Score = 201 bits (511), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 98/98 (100%), Positives = 98/98 (100%) Query: 361 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 420 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN Sbjct: 4 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 63 Query: 421 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 458 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL Sbjct: 64 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 101 >5D1Y-A nrPDB Length = 812 Score = 30.0 bits (66), Expect = 2.1, Method: Compositional matrix adjust. Identities = 24/77 (31%), Positives = 37/77 (48%), Gaps = 12/77 (15%) Query: 148 KHPSLPVLITRIQLFDSNNLFLSTPNIGSINKESLYNKLDKFQGK--PLISRRPYYVAFP 205 KHP +L RI + +NL T + S E LYN ++ GK P++++ + Sbjct: 90 KHPDYAILAARIAV---SNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLA 146 Query: 206 ----LNSPIIFHSVDKD 218 LNS II+ D+D Sbjct: 147 NKDRLNSAIIY---DRD 160 >2WBK-A nrPDB Length = 847 Score = 28.5 bits (62), Expect = 7.2, Method: Compositional matrix adjust. Identities = 17/50 (34%), Positives = 27/50 (54%) Query: 145 YMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKESLYNKLDKFQGKPL 194 Y K PVLI IQ DS +++L + + ++ + +L K+ F GK L Sbjct: 645 YQAKRAFAPVLINPIQQNDSLSVYLISDRLDTMEQMTLEMKVVDFDGKTL 694 >2E5V-A nrPDB Length = 472 Score = 28.1 bits (61), Expect = 8.4, Method: Compositional matrix adjust. Identities = 13/29 (44%), Positives = 18/29 (62%) Query: 30 TVLYDLTLSWFAKFGGSFDGDIYLLTETL 58 T+L D+ F S DG+++LLTETL Sbjct: 201 TILADMEFVQFHPTVTSLDGEVFLLTETL 229 >3CZQ-A nrPDB Length = 304 Score = 28.1 bits (61), Expect = 9.4, Method: Compositional matrix adjust. Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 3/45 (6%) Query: 98 ISKPEKFKWLPSKALRGDGKPYVVKLQPAKFIEN---LQTDLAKI 139 I P+ KW+ +A R D PY KL ++ E LQ +L K+ Sbjct: 32 IDDPDLPKWIDEEAFRSDDYPYKKKLDREEYEETLTKLQIELVKV 76 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6NUW-E nrPDB 977 0.0 4JE3-B nrPDB 210 1e-54 Sequences not found previously or not previously below threshold: 5D1Y-A nrPDB 31 0.99 3CZQ-A nrPDB 30 2.1 2WBK-A nrPDB 29 4.2 3T6J-A nrPDB 29 4.8 2E5V-A nrPDB 29 5.7 3KBB-A nrPDB 28 6.3 CONVERGED! >6NUW-E nrPDB Length = 461 Score = 977 bits (2527), Expect = 0.0, Method: Composition-based stats. Identities = 458/458 (100%), Positives = 458/458 (100%) Query: 1 MSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTETLDL 60 MSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTETLDL Sbjct: 4 MSNELRLEDNYVPTSDTLVVFKQLMKLPVTVLYDLTLSWFAKFGGSFDGDIYLLTETLDL 63 Query: 61 LIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGKPYV 120 LIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGKPYV Sbjct: 64 LIEKGVRRNVIVNRILYVYWPDGLNVFQLAEIDCHLMISKPEKFKWLPSKALRGDGKPYV 123 Query: 121 VKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKE 180 VKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKE Sbjct: 124 VKLQPAKFIENLQTDLAKIYHCHVYMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKE 183 Query: 181 SLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERETIIF 240 SLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERETIIF Sbjct: 184 SLYNKLDKFQGKPLISRRPYYVAFPLNSPIIFHSVDKDIYARLVLQSISRTISERETIIF 243 Query: 241 KPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKVMVR 300 KPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKVMVR Sbjct: 244 KPVQKIPVKSIHNIMTLLGPSRFAESMGPWECYASANFERSPLHDYKKHQGLTGKKVMVR 303 Query: 301 EFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHNIRN 360 EFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHNIRN Sbjct: 304 EFDDSFLNDDENFYGKEEPEIRRLRLEKNMIKFKGSANGVMDQKYNDLKEFNEHVHNIRN 363 Query: 361 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 420 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN Sbjct: 364 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 423 Query: 421 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 458 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL Sbjct: 424 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 461 >4JE3-B nrPDB Length = 101 Score = 210 bits (534), Expect = 1e-54, Method: Composition-based stats. Identities = 98/98 (100%), Positives = 98/98 (100%) Query: 361 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 420 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN Sbjct: 4 GKKNEDSGEPVYISRYSSLVPIEKVGFTLKNEINSRIITIKLKFNGNDIFGGLHELCDKN 63 Query: 421 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 458 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL Sbjct: 64 LINIDKVPGWLAGENGSFSGTIMNGDFQREQVAKGGLL 101 >5D1Y-A nrPDB Length = 812 Score = 31.1 bits (69), Expect = 0.99, Method: Composition-based stats. Identities = 26/91 (28%), Positives = 43/91 (47%), Gaps = 13/91 (14%) Query: 148 KHPSLPVLITRIQLFDSNNLFLSTPNIGSINKESLYNKLDKFQGK--PLISRRPYYVAFP 205 KHP +L RI + +NL T + S E LYN ++ GK P++++ + Sbjct: 90 KHPDYAILAARIAV---SNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLA 146 Query: 206 ----LNSPIIFHSVDKDI-YARLVLQSISRT 231 LNS II+ D+D Y +++ R+ Sbjct: 147 NKDRLNSAIIY---DRDFSYNYFGFKTLERS 174 >3CZQ-A nrPDB Length = 304 Score = 30.0 bits (66), Expect = 2.1, Method: Composition-based stats. Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 3/45 (6%) Query: 98 ISKPEKFKWLPSKALRGDGKPYVVKLQPAKFIEN---LQTDLAKI 139 I P+ KW+ +A R D PY KL ++ E LQ +L K+ Sbjct: 32 IDDPDLPKWIDEEAFRSDDYPYKKKLDREEYEETLTKLQIELVKV 76 >2WBK-A nrPDB Length = 847 Score = 29.2 bits (64), Expect = 4.2, Method: Composition-based stats. Identities = 17/50 (34%), Positives = 27/50 (54%) Query: 145 YMFKHPSLPVLITRIQLFDSNNLFLSTPNIGSINKESLYNKLDKFQGKPL 194 Y K PVLI IQ DS +++L + + ++ + +L K+ F GK L Sbjct: 645 YQAKRAFAPVLINPIQQNDSLSVYLISDRLDTMEQMTLEMKVVDFDGKTL 694 >3T6J-A nrPDB Length = 726 Score = 28.8 bits (63), Expect = 4.8, Method: Composition-based stats. Identities = 24/89 (26%), Positives = 42/89 (47%), Gaps = 6/89 (6%) Query: 318 EPEIRRLRLEKNMIKFKGSANGVMDQK---YNDLKEFNEHVHNIRNGKKNEDSGEPVYIS 374 EP +R L L K I S N M+ + L N +N R K+ + G+P Y Sbjct: 155 EPRLRHLGLGKEGITTYFSGNCTMEDAKLAQDFLDSQNLSAYNTRLFKEVDGEGKPYYEV 214 Query: 375 RYSSLVPIEKVGFTLKNEINSRIITIKLK 403 R +S++ E +L +E+ S++ + + + Sbjct: 215 RLASVLGSEP---SLDSEVTSKLKSYEFR 240 >2E5V-A nrPDB Length = 472 Score = 28.8 bits (63), Expect = 5.7, Method: Composition-based stats. Identities = 36/115 (31%), Positives = 52/115 (45%), Gaps = 30/115 (26%) Query: 30 TVLYDLTLSWFAKFGGSFDGDIYLLTETL-----DLLIEKGVR-------------RNVI 71 T+L D+ F S DG+++LLTETL ++ E G R R+ I Sbjct: 201 TILADMEFVQFHPTVTSLDGEVFLLTETLRGEGAQIINENGERFLFNYDKRGELAPRD-I 259 Query: 72 VNRILYVYWPDGLNVFQLAEIDCHLMISKPEKF-KWLP--SKALRGDGKPYVVKL 123 ++R +Y+ G VF ID +SK E F + P +K L G Y VK+ Sbjct: 260 LSRAIYIEMLKGHKVF----ID----LSKIEDFERKFPVVAKYLARHGHNYKVKI 306 >3KBB-A nrPDB Length = 216 Score = 28.4 bits (62), Expect = 6.3, Method: Composition-based stats. Identities = 20/80 (25%), Positives = 41/80 (51%), Gaps = 6/80 (7%) Query: 295 KKVMVREFDDSFLNDDENFYGKEEPEIRRLRLEK------NMIKFKGSANGVMDQKYNDL 348 +++ + ++ D + D+ GK +PEI L LE+ ++ F+ S +GV K + Sbjct: 119 RRLDLEKYFDVMVFGDQVKNGKPDPEIYLLVLERLNVVPEKVVVFEDSKSGVEAAKSAGI 178 Query: 349 KEFNEHVHNIRNGKKNEDSG 368 + VH++ +GK ++G Sbjct: 179 ERIYGVVHSLNDGKALLEAG 198 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.140 0.416 Lambda K H 0.267 0.0431 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,150,538 Number of Sequences: 36641 Number of extensions: 928752 Number of successful extensions: 2062 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 2049 Number of HSP's gapped (non-prelim): 23 length of query: 458 length of database: 10,336,785 effective HSP length: 100 effective length of query: 358 effective length of database: 6,672,685 effective search space: 2388821230 effective search space used: 2388821230 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 61 (28.0 bits)