BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38826 (435 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5V8F-F nrPDB 904 0.0 3SQG-A nrPDB 33 0.17 2VCT-A nrPDB 30 1.9 1PL2-A nrPDB 30 3.0 3I6A-A nrPDB 29 3.3 >5V8F-F nrPDB Length = 435 Score = 904 bits (2335), Expect = 0.0, Method: Compositional matrix adjust. Identities = 435/435 (100%), Positives = 435/435 (100%) Query: 1 MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHI 60 MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHI Sbjct: 1 MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHI 60 Query: 61 CAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKN 120 CAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKN Sbjct: 61 CAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKN 120 Query: 121 KRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKL 180 KRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKL Sbjct: 121 KRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKL 180 Query: 181 AFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRS 240 AFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRS Sbjct: 181 AFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRS 240 Query: 241 GRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAY 300 GRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAY Sbjct: 241 GRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAY 300 Query: 301 KIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVD 360 KIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVD Sbjct: 301 KIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVD 360 Query: 361 DVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNI 420 DVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNI Sbjct: 361 DVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNI 420 Query: 421 WKKRIEMDLALTEPL 435 WKKRIEMDLALTEPL Sbjct: 421 WKKRIEMDLALTEPL 435 >3SQG-A nrPDB Length = 579 Score = 33.5 bits (75), Expect = 0.17, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 21/46 (45%) Query: 283 EEIIRLCNDFELPREVAYKIVDEYNINASRLVCPWQLVCGLVLNCT 328 EEIIR ND+ L + AY V E + S C CG + C Sbjct: 390 EEIIRAENDYALTQYEAYPTVAESHFGGSVRACCAAAGCGSAVACA 435 >2VCT-A nrPDB Length = 222 Score = 30.0 bits (66), Expect = 1.9, Method: Compositional matrix adjust. Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 10/112 (8%) Query: 10 VAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHICAYIASQKM 69 + E++ L P +P+ L + T Y + KV+ + H Y+ K+ Sbjct: 102 LGEMILLLPFTQPEEQDAKLALIQEKTKNRYFPAFEKVL--------KSHGQDYLVGNKL 153 Query: 70 NEK--HMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKK 119 + H+ +L YY++ + + L+ + +SN +K+F SP+K Sbjct: 154 SRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRK 205 >1PL2-A nrPDB Length = 222 Score = 29.6 bits (65), Expect = 3.0, Method: Compositional matrix adjust. Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Query: 53 EEVARCHICAYIASQKMNEK--HMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQ 110 E+V + H Y+ K++ H+ +L YY++ + + L+ + +SN +K+ Sbjct: 137 EKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKK 196 Query: 111 FAWTPSPKK 119 F SP+K Sbjct: 197 FLQPGSPRK 205 >3I6A-A nrPDB Length = 222 Score = 29.3 bits (64), Expect = 3.3, Method: Compositional matrix adjust. Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Query: 53 EEVARCHICAYIASQKMNEK--HMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQ 110 E+V + H Y+ K++ H+ +L YY++ + + L+ + +SN +K+ Sbjct: 137 EKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKK 196 Query: 111 FAWTPSPKK 119 F SP+K Sbjct: 197 FLQPGSPRK 205 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5V8F-F nrPDB 939 0.0 Sequences not found previously or not previously below threshold: 6FF7-0 nrPDB 36 0.029 2VCT-A nrPDB 32 0.48 5XF8-3 nrPDB 32 0.63 3I6A-A nrPDB 31 1.2 1PL2-A nrPDB 31 1.2 3SQG-A nrPDB 30 1.4 CONVERGED! >5V8F-F nrPDB Length = 435 Score = 939 bits (2426), Expect = 0.0, Method: Composition-based stats. Identities = 435/435 (100%), Positives = 435/435 (100%) Query: 1 MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHI 60 MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHI Sbjct: 1 MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHI 60 Query: 61 CAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKN 120 CAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKN Sbjct: 61 CAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKN 120 Query: 121 KRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKL 180 KRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKL Sbjct: 121 KRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKL 180 Query: 181 AFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRS 240 AFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRS Sbjct: 181 AFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRS 240 Query: 241 GRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAY 300 GRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAY Sbjct: 241 GRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAY 300 Query: 301 KIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVD 360 KIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVD Sbjct: 301 KIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVD 360 Query: 361 DVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNI 420 DVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNI Sbjct: 361 DVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNI 420 Query: 421 WKKRIEMDLALTEPL 435 WKKRIEMDLALTEPL Sbjct: 421 WKKRIEMDLALTEPL 435 >6FF7-0 nrPDB Length = 396 Score = 36.2 bits (82), Expect = 0.029, Method: Composition-based stats. Identities = 39/160 (24%), Positives = 61/160 (38%), Gaps = 25/160 (15%) Query: 114 TPSPKKNKRSPVKNG-----GRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQ 168 +PSP ++ P ++G R S P + +N+ G R+S++ P + Sbjct: 52 SPSPPTSE--PARSGHRGNRARGVSRSPPKKKNKASG-----RRSKS---------PRSK 95 Query: 169 TNESPSITRRKLAFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESD-PT 227 N SP + K+ + E ED G + + S + RN D D + H T Sbjct: 96 RNRSPHHSTVKV---KQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRT 152 Query: 228 SEEEPLGVQESRSGRTKQNKAVGKPQSELKTAKALRKRGR 267 S E P Q R QN + + E A+ R R Sbjct: 153 SNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQR 192 >2VCT-A nrPDB Length = 222 Score = 32.0 bits (71), Expect = 0.48, Method: Composition-based stats. Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 10/112 (8%) Query: 10 VAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHICAYIASQKM 69 + E++ L P +P+ L + T Y + KV+ + H Y+ K+ Sbjct: 102 LGEMILLLPFTQPEEQDAKLALIQEKTKNRYFPAFEKVL--------KSHGQDYLVGNKL 153 Query: 70 NEK--HMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKK 119 + H+ +L YY++ + + L+ + +SN +K+F SP+K Sbjct: 154 SRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRK 205 >5XF8-3 nrPDB Length = 997 Score = 31.6 bits (70), Expect = 0.63, Method: Composition-based stats. Identities = 27/99 (27%), Positives = 39/99 (39%), Gaps = 30/99 (30%) Query: 101 SLSNSSPMKQFAWTPSPKKNKRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFV 160 +LS SP K +P ++ R P N G S P+ T + V Sbjct: 782 ALSKRSPQK----SPKKRQRVRQPASNSGSPIKSTPRR------STASSV---------- 821 Query: 161 IPELPPMQTNESPSITRRKLAFEEDEDEDEEEPGNDGLS 199 N +PS RR L F++DE ++ E ND +S Sbjct: 822 ---------NATPSSARRILRFQDDE-QNAGEDDNDIMS 850 >3I6A-A nrPDB Length = 222 Score = 30.8 bits (68), Expect = 1.2, Method: Composition-based stats. Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Query: 53 EEVARCHICAYIASQKMNEK--HMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQ 110 E+V + H Y+ K++ H+ +L YY++ + + L+ + +SN +K+ Sbjct: 137 EKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKK 196 Query: 111 FAWTPSPKK 119 F SP+K Sbjct: 197 FLQPGSPRK 205 >1PL2-A nrPDB Length = 222 Score = 30.8 bits (68), Expect = 1.2, Method: Composition-based stats. Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Query: 53 EEVARCHICAYIASQKMNEK--HMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQ 110 E+V + H Y+ K++ H+ +L YY++ + + L+ + +SN +K+ Sbjct: 137 EKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKK 196 Query: 111 FAWTPSPKK 119 F SP+K Sbjct: 197 FLQPGSPRK 205 >3SQG-A nrPDB Length = 579 Score = 30.4 bits (67), Expect = 1.4, Method: Composition-based stats. Identities = 17/45 (37%), Positives = 21/45 (46%) Query: 283 EEIIRLCNDFELPREVAYKIVDEYNINASRLVCPWQLVCGLVLNC 327 EEIIR ND+ L + AY V E + S C CG + C Sbjct: 390 EEIIRAENDYALTQYEAYPTVAESHFGGSVRACCAAAGCGSAVAC 434 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.133 0.389 Lambda K H 0.267 0.0413 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,980,562 Number of Sequences: 36641 Number of extensions: 689927 Number of successful extensions: 2164 Number of sequences better than 10.0: 26 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 46 Number of HSP's that attempted gapping in prelim test: 2079 Number of HSP's gapped (non-prelim): 104 length of query: 435 length of database: 10,336,785 effective HSP length: 100 effective length of query: 335 effective length of database: 6,672,685 effective search space: 2235349475 effective search space used: 2235349475 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 61 (28.1 bits)