BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38801 (184 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5C0W-L nrPDB 367 e-102 5CJE-A nrPDB 35 0.025 6GK6-A nrPDB 30 0.63 1Z8P-A nrPDB 27 4.9 3FN9-A nrPDB 26 7.7 >5C0W-L nrPDB Length = 184 Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/184 (100%), Positives = 184/184 (100%) Query: 1 MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL 60 MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL Sbjct: 1 MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL 60 Query: 61 MFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD 120 MFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD Sbjct: 61 MFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD 120 Query: 121 GNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTDHIRKASSKKSKRLDKVGKKK 180 GNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTDHIRKASSKKSKRLDKVGKKK Sbjct: 121 GNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTDHIRKASSKKSKRLDKVGKKK 180 Query: 181 GGKK 184 GGKK Sbjct: 181 GGKK 184 >5CJE-A nrPDB Length = 393 Score = 34.7 bits (78), Expect = 0.025, Method: Compositional matrix adjust. Identities = 23/88 (26%), Positives = 43/88 (48%), Gaps = 24/88 (27%) Query: 3 DIEKIKPYV----------------RSFS-KALDELKPEIEKLTSKSLDEQLLLLSDERA 45 D+E + PY+ R+F+ + ++ L+P I+++T LDE L R Sbjct: 75 DVELMGPYLLVVDPPEHTRLRSLVARAFTMRRVEALRPRIQEITDGLLDEML-----PRG 129 Query: 46 KLELINRYAYVLSSLMFANMKVLGVKDM 73 + +L++ +AY L + + LGV D+ Sbjct: 130 RADLVDSFAYPLPITVICEL--LGVPDI 155 >6GK6-A nrPDB Length = 411 Score = 30.0 bits (66), Expect = 0.63, Method: Compositional matrix adjust. Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 6/62 (9%) Query: 12 RSFS-KALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSLMFANMKVLGV 70 R+FS + ++ L+P I+++ +D + +R ++ELI +A+ L + + A + L V Sbjct: 108 RAFSPRMMEGLRPRIQEIADGLIDAVI-----DRRRMELIADFAFPLPTAVIAELLGLPV 162 Query: 71 KD 72 +D Sbjct: 163 ED 164 >1Z8P-A nrPDB Length = 404 Score = 26.9 bits (58), Expect = 4.9, Method: Compositional matrix adjust. Identities = 20/103 (19%), Positives = 51/103 (49%), Gaps = 18/103 (17%) Query: 18 LDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSLMFANMKVLGVKDMSPIL 77 ++ ++P +E++T++ LDE + +++++R+A+ L + + LGV + Sbjct: 114 VEAMRPRVEQITAELLDE-----VGDSGVVDIVDRFAHPLPIKVICEL--LGVDEK--YR 164 Query: 78 GELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD 120 GE R S + + E+++ + A+ +++ +LD Sbjct: 165 GEFGRWSSEI---------LVMDPERAEQRGQAAREVVNFILD 198 >3FN9-A nrPDB Length = 692 Score = 26.2 bits (56), Expect = 7.7, Method: Composition-based stats. Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 1/58 (1%) Query: 3 DIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL 60 DI+ + Y + K + ++KP +E+L K Q L+L++ A L ++ Y+ +L Sbjct: 446 DIQGMNRYFGWYEKKIQDIKPWVEQL-EKDYPYQKLMLTEYGADANLAHQTEYLGDAL 502 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5C0W-L nrPDB 313 3e-86 Sequences not found previously or not previously below threshold: 5CJE-A nrPDB 33 0.064 6GK6-A nrPDB 31 0.44 3US9-A nrPDB 30 0.72 2FWU-A nrPDB 29 1.1 2MSV-A nrPDB 28 2.3 3FN9-A nrPDB 28 2.5 1Z8P-A nrPDB 28 2.6 3PDK-A nrPDB 27 4.4 5WOY-A nrPDB 27 4.4 CONVERGED! >5C0W-L nrPDB Length = 184 Score = 313 bits (801), Expect = 3e-86, Method: Composition-based stats. Identities = 184/184 (100%), Positives = 184/184 (100%) Query: 1 MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL 60 MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL Sbjct: 1 MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL 60 Query: 61 MFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD 120 MFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD Sbjct: 61 MFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD 120 Query: 121 GNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTDHIRKASSKKSKRLDKVGKKK 180 GNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTDHIRKASSKKSKRLDKVGKKK Sbjct: 121 GNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTDHIRKASSKKSKRLDKVGKKK 180 Query: 181 GGKK 184 GGKK Sbjct: 181 GGKK 184 >5CJE-A nrPDB Length = 393 Score = 33.2 bits (74), Expect = 0.064, Method: Composition-based stats. Identities = 23/91 (25%), Positives = 44/91 (48%), Gaps = 24/91 (26%) Query: 3 DIEKIKPYV----------------RSFS-KALDELKPEIEKLTSKSLDEQLLLLSDERA 45 D+E + PY+ R+F+ + ++ L+P I+++T LDE L R Sbjct: 75 DVELMGPYLLVVDPPEHTRLRSLVARAFTMRRVEALRPRIQEITDGLLDEML-----PRG 129 Query: 46 KLELINRYAYVLSSLMFANMKVLGVKDMSPI 76 + +L++ +AY L + + LGV D+ + Sbjct: 130 RADLVDSFAYPLPITVICEL--LGVPDIDRV 158 >6GK6-A nrPDB Length = 411 Score = 30.5 bits (67), Expect = 0.44, Method: Composition-based stats. Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 6/62 (9%) Query: 12 RSFS-KALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSLMFANMKVLGV 70 R+FS + ++ L+P I+++ +D + +R ++ELI +A+ L + + A + L V Sbjct: 108 RAFSPRMMEGLRPRIQEIADGLIDAVI-----DRRRMELIADFAFPLPTAVIAELLGLPV 162 Query: 71 KD 72 +D Sbjct: 163 ED 164 >3US9-A nrPDB Length = 295 Score = 29.7 bits (65), Expect = 0.72, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 27/45 (60%), Gaps = 3/45 (6%) Query: 141 KFENDELAESTTTKIIDSTDHIRKAS---SKKSKRLDKVGKKKGG 182 +F+NDE+ ++ + K+ID ++ + + RL ++ +KKGG Sbjct: 199 EFQNDEIVKTISVKVIDDEEYEKNKTFFLEIGEPRLVEMSEKKGG 243 >2FWU-A nrPDB Length = 157 Score = 29.0 bits (63), Expect = 1.1, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 27/45 (60%), Gaps = 3/45 (6%) Query: 141 KFENDELAESTTTKIIDSTDHIRKAS---SKKSKRLDKVGKKKGG 182 +F+NDE+ ++ + K+ID ++ + + RL ++ +KKGG Sbjct: 61 EFQNDEIVKTISVKVIDDEEYEKNKTFFLEIGEPRLVEMSEKKGG 105 >2MSV-A nrPDB Length = 166 Score = 28.2 bits (61), Expect = 2.3, Method: Composition-based stats. Identities = 15/48 (31%), Positives = 26/48 (54%) Query: 5 EKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINR 52 EK+ + F AL+E EIEK +++S + L S ++ + +NR Sbjct: 68 EKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNR 115 >3FN9-A nrPDB Length = 692 Score = 27.8 bits (60), Expect = 2.5, Method: Composition-based stats. Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 1/58 (1%) Query: 3 DIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSL 60 DI+ + Y + K + ++KP +E+L K Q L+L++ A L ++ Y+ +L Sbjct: 446 DIQGMNRYFGWYEKKIQDIKPWVEQL-EKDYPYQKLMLTEYGADANLAHQTEYLGDAL 502 >1Z8P-A nrPDB Length = 404 Score = 27.8 bits (60), Expect = 2.6, Method: Composition-based stats. Identities = 20/105 (19%), Positives = 52/105 (49%), Gaps = 18/105 (17%) Query: 16 KALDELKPEIEKLTSKSLDEQLLLLSDERAKLELINRYAYVLSSLMFANMKVLGVKDMSP 75 + ++ ++P +E++T++ LDE + +++++R+A+ L + + LGV + Sbjct: 112 RRVEAMRPRVEQITAELLDE-----VGDSGVVDIVDRFAHPLPIKVICEL--LGVDE--K 162 Query: 76 ILGELKRVKSYMDKAKQYDNRITKSNEKSQAEQEKAKNIISNVLD 120 GE R S + + E+++ + A+ +++ +LD Sbjct: 163 YRGEFGRWSSEI---------LVMDPERAEQRGQAAREVVNFILD 198 >3PDK-A nrPDB Length = 469 Score = 27.0 bits (58), Expect = 4.4, Method: Composition-based stats. Identities = 12/48 (25%), Positives = 24/48 (50%) Query: 56 VLSSLMFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNEK 103 +LS+L N+ + K +S + GE+ + + + D ++ NEK Sbjct: 363 MLSALQLVNIMKMTKKPLSELAGEMTKFPQLLVNVRVTDKKLALENEK 410 >5WOY-A nrPDB Length = 248 Score = 27.0 bits (58), Expect = 4.4, Method: Composition-based stats. Identities = 15/37 (40%), Positives = 23/37 (62%), Gaps = 2/37 (5%) Query: 1 MEDIE--KIKPYVRSFSKALDELKPEIEKLTSKSLDE 35 +E IE K++ + F KAL+ + EIEK+ K+L E Sbjct: 28 VEKIEESKVEEEIAKFRKALEVTQEEIEKIKEKALKE 64 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.126 0.325 Lambda K H 0.267 0.0399 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,199,000 Number of Sequences: 36641 Number of extensions: 219388 Number of successful extensions: 775 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 41 Number of HSP's that attempted gapping in prelim test: 753 Number of HSP's gapped (non-prelim): 60 length of query: 184 length of database: 10,336,785 effective HSP length: 91 effective length of query: 93 effective length of database: 7,002,454 effective search space: 651228222 effective search space used: 651228222 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 56 (26.2 bits)