BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P38786 (293 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AGB-I nrPDB 597 e-171 6AHU-I nrPDB 100 7e-22 4F0U-A nrPDB 30 0.92 1B33-A nrPDB 30 1.5 4TRT-A nrPDB 29 2.3 6IRS-B nrPDB 28 3.2 5W21-A nrPDB 28 5.0 >6AGB-I nrPDB Length = 293 Score = 597 bits (1539), Expect = e-171, Method: Compositional matrix adjust. Identities = 293/293 (100%), Positives = 293/293 (100%) Query: 1 MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLL 60 MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLL Sbjct: 1 MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLL 60 Query: 61 NPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLS 120 NPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLS Sbjct: 61 NPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLS 120 Query: 121 TTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSV 180 TTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSV Sbjct: 121 TTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSV 180 Query: 181 IRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRN 240 IRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRN Sbjct: 181 IRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRN 240 Query: 241 KSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP 293 KSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP Sbjct: 241 KSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP 293 >6AHU-I nrPDB Length = 268 Score = 100 bits (249), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 66/229 (28%), Positives = 115/229 (50%), Gaps = 13/229 (5%) Query: 21 QAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLLNPIDIKRRFGELMDRTG--- 77 +A+ L++T + LGY+ +AIN V+ EK ++ P+ + F L G Sbjct: 16 KALRGLVETAA---HLGYSVVAINHIVDFKEK---KQEIEKPVAVSELFTTLPIVQGKSR 69 Query: 78 -LKLYSRITLIIDDPSKGQSLSKIS---QAFDIVAALPISEKGLTLSTTNLDIDLLTFQY 133 +K+ +R+T+I+ DPS L S + +D+VA P +EK ++ T+LD+DL+ Sbjct: 70 PIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITV 129 Query: 134 GSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSVIRSSRSRGIVIGS 193 +LP + K I ++RG+ E+VY A++D RR +S+ ++++ + + ++I S Sbjct: 130 TEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISS 189 Query: 194 GAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRNKS 242 A PLE R V +L GL A+ LL+G R + Sbjct: 190 AAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTA 238 >4F0U-A nrPDB Length = 160 Score = 30.4 bits (67), Expect = 0.92, Method: Compositional matrix adjust. Identities = 16/47 (34%), Positives = 28/47 (59%), Gaps = 2/47 (4%) Query: 193 SGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDL--ASLVLLNGR 237 SG ++P+E I+GV + K+LG P + ++ + +L A+ LL G Sbjct: 97 SGDITPIEEIGIVGVREMYKSLGTPIEAVAEGVRELKSAATALLTGE 143 >1B33-A nrPDB Length = 160 Score = 29.6 bits (65), Expect = 1.5, Method: Compositional matrix adjust. Identities = 19/49 (38%), Positives = 30/49 (61%), Gaps = 5/49 (10%) Query: 188 GIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCS---KAMGDLASLVL 233 GIV +G ++P+E I+GV + K+LG P D + AM ++AS +L Sbjct: 94 GIV--AGDVTPIEEIGIVGVREMYKSLGTPIDAVAAGVSAMKNVASSIL 140 >4TRT-A nrPDB Length = 368 Score = 29.3 bits (64), Expect = 2.3, Method: Compositional matrix adjust. Identities = 14/19 (73%), Positives = 15/19 (78%) Query: 113 SEKGLTLSTTNLDIDLLTF 131 SE GLTLS TNL+IDL F Sbjct: 39 SEGGLTLSGTNLEIDLSCF 57 >6IRS-B nrPDB Length = 527 Score = 28.5 bits (62), Expect = 3.2, Method: Compositional matrix adjust. Identities = 18/39 (46%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Query: 104 FDIVAALPISEKGLTLSTTNLDIDLLTFQYGSRLPTFLK 142 F IV AL +E G T+S + D + YGS LP FLK Sbjct: 115 FSIVGALCYAELGTTISKSGGDYAYMLEVYGS-LPAFLK 152 >5W21-A nrPDB Length = 981 Score = 28.1 bits (61), Expect = 5.0, Method: Composition-based stats. Identities = 15/51 (29%), Positives = 22/51 (43%), Gaps = 2/51 (3%) Query: 5 LNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPN 55 L P P V S + NN+ + L LG TH F+++ + PN Sbjct: 111 LGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTH--YRFSISWARVLPN 159 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AGB-I nrPDB 497 e-141 6AHU-I nrPDB 329 9e-91 Sequences not found previously or not previously below threshold: 3WZ0-E nrPDB 36 0.022 1ZTY-A nrPDB 28 3.8 5X3F-B nrPDB 28 4.5 2Z49-A nrPDB 28 4.6 4L82-A nrPDB 28 5.4 4TRT-A nrPDB 28 6.1 2GKO-A nrPDB 27 8.1 2VAP-A nrPDB 27 8.4 CONVERGED! >6AGB-I nrPDB Length = 293 Score = 497 bits (1279), Expect = e-141, Method: Composition-based stats. Identities = 293/293 (100%), Positives = 293/293 (100%) Query: 1 MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLL 60 MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLL Sbjct: 1 MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLL 60 Query: 61 NPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLS 120 NPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLS Sbjct: 61 NPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQAFDIVAALPISEKGLTLS 120 Query: 121 TTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSV 180 TTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSV Sbjct: 121 TTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSV 180 Query: 181 IRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRN 240 IRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRN Sbjct: 181 IRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRN 240 Query: 241 KSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP 293 KSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP Sbjct: 241 KSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQLGHASKRHKP 293 >6AHU-I nrPDB Length = 268 Score = 329 bits (844), Expect = 9e-91, Method: Composition-based stats. Identities = 66/229 (28%), Positives = 115/229 (50%), Gaps = 13/229 (5%) Query: 21 QAVNNLIKTLSTLHMLGYTHIAINFTVNHSEKFPNDVKLLNPIDIKRRFGELMDRTG--- 77 +A+ L++T + LGY+ +AIN V+ EK ++ P+ + F L G Sbjct: 16 KALRGLVETAA---HLGYSVVAINHIVDFKEK---KQEIEKPVAVSELFTTLPIVQGKSR 69 Query: 78 -LKLYSRITLIIDDPSKGQSLSKIS---QAFDIVAALPISEKGLTLSTTNLDIDLLTFQY 133 +K+ +R+T+I+ DPS L S + +D+VA P +EK ++ T+LD+DL+ Sbjct: 70 PIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITV 129 Query: 134 GSRLPTFLKHKSICSCVNRGVKLEIVYGYALRDVQARRQFVSNVRSVIRSSRSRGIVIGS 193 +LP + K I ++RG+ E+VY A++D RR +S+ ++++ + + ++I S Sbjct: 130 TEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISS 189 Query: 194 GAMSPLECRNILGVTSLIKNLGLPSDRCSKAMGDLASLVLLNGRLRNKS 242 A PLE R V +L GL A+ LL+G R + Sbjct: 190 AAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTA 238 >3WZ0-E nrPDB Length = 220 Score = 35.8 bits (81), Expect = 0.022, Method: Composition-based stats. Identities = 24/100 (24%), Positives = 38/100 (38%), Gaps = 6/100 (6%) Query: 128 LLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVYGYALR-DVQARRQFVSNVRSVIRSSRS 186 L++ +G + P F H RGV + L + R Q + + + + Sbjct: 110 LISPWFGRKDPGF-DHTLAGMAARRGVAIGFSLSPLLNANPYGRAQILRFMMKTWQLVKK 168 Query: 187 RGI--VIGSGAMSPLECRNILGVTSLIKNLGL--PSDRCS 222 + I S A S E R + SL N+G+ P R S Sbjct: 169 YRVPRFITSSAESRWEVRGPRDLMSLGINIGMEIPEARAS 208 >1ZTY-A nrPDB Length = 529 Score = 28.5 bits (62), Expect = 3.8, Method: Composition-based stats. Identities = 20/84 (23%), Positives = 35/84 (41%), Gaps = 15/84 (17%) Query: 10 PQNSYADKVTSQAVNNLIK-TLSTLHMLGYTHIAIN--------------FTVNHSEKFP 54 PQ + D++ + VN+ + T S + + T+ A N F VN P Sbjct: 198 PQIANNDQLLGKIVNSELDWTSSFVPDIDRTYAAANPNHHYWYPAAGTQAFMVNFKNPDP 257 Query: 55 NDVKLLNPIDIKRRFGELMDRTGL 78 + L+ +D +R F +DR + Sbjct: 258 AKKEALDNVDFRRAFSMALDRQTI 281 >5X3F-B nrPDB Length = 393 Score = 28.1 bits (61), Expect = 4.5, Method: Composition-based stats. Identities = 11/48 (22%), Positives = 26/48 (54%), Gaps = 1/48 (2%) Query: 71 ELMDRTGLKLYSRITLIIDDPSK-GQSLSKISQAFDIVAALPISEKGL 117 L + G ++ + I+ ++DDPS+ L++ + D+ A L +++ Sbjct: 22 TLPNLNGRQVAAFISSLLDDPSQSANLLAEAKKLNDVQAFLAKAKEDF 69 >2Z49-A nrPDB Length = 432 Score = 28.1 bits (61), Expect = 4.6, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 21/45 (46%) Query: 228 LASLVLLNGRLRNKSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTI 272 L + L G LR+ KQ + G G+G+ D G+ D Q I Sbjct: 3 LCTNPLDIGELRSFKSKQCVDIVGNQGSGNIATYDCDGLSDQQII 47 >4L82-A nrPDB Length = 168 Score = 28.1 bits (61), Expect = 5.4, Method: Composition-based stats. Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 3/52 (5%) Query: 101 SQAFDIVAALPISEKGLTLSTTNLDIDLLTFQYGSRLPTFLKHKSICSCVNR 152 SQ D ++ P +G+T+ TTN + +L F S LK I C+N+ Sbjct: 16 SQFKDTMSRFP---QGVTIITTNCNNELFGFTASSLTSVSLKPPLILFCLNK 64 >4TRT-A nrPDB Length = 368 Score = 27.7 bits (60), Expect = 6.1, Method: Composition-based stats. Identities = 14/19 (73%), Positives = 15/19 (78%) Query: 113 SEKGLTLSTTNLDIDLLTF 131 SE GLTLS TNL+IDL F Sbjct: 39 SEGGLTLSGTNLEIDLSCF 57 >2GKO-A nrPDB Length = 309 Score = 27.3 bits (59), Expect = 8.1, Method: Composition-based stats. Identities = 12/38 (31%), Positives = 22/38 (57%) Query: 224 AMGDLASLVLLNGRLRNKSHKQTIVTGGGSGNGDDVVN 261 A AS V + G L+ ++ I++G +G+GDD+ + Sbjct: 264 AQSPAASNVDVRGELQTRASVNDILSGNSAGSGDDIAS 301 >2VAP-A nrPDB Length = 364 Score = 27.3 bits (59), Expect = 8.4, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 26/47 (55%), Gaps = 3/47 (6%) Query: 238 LRNKSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVKRSMDAEQL 284 L+ K T+V GG+GN ++GI+ +T+ + + DA+QL Sbjct: 33 LQQTKAKITVVGCGGAGNNTITRLKMEGIEGAKTVAI---NTDAQQL 76 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.136 0.362 Lambda K H 0.267 0.0418 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,813,471 Number of Sequences: 36641 Number of extensions: 421185 Number of successful extensions: 1087 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 1067 Number of HSP's gapped (non-prelim): 25 length of query: 293 length of database: 10,336,785 effective HSP length: 96 effective length of query: 197 effective length of database: 6,819,249 effective search space: 1343392053 effective search space used: 1343392053 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.3 bits)